YJR047C / ANB1

Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR047C / ANB1

Click on Significant Values for Screen Details ID:SGTC_55|Compound:0767-0285|FD-Score:3.54|P-value:1.98E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.12|P-value:1.87E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:3.40|P-value:3.43E-4 ID:SGTC_357|Compound:1325-0123|FD-Score:-4.41|P-value:4.56E-6 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:3.73|P-value:9.64E-5 ID:SGTC_783|Compound:0848-0141|FD-Score:3.24|P-value:6.15E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:3.19|P-value:7.30E-4 ID:SGTC_1223|Compound:0764-0573|FD-Score:3.79|P-value:7.67E-5 ID:SGTC_1298|Compound:1079-1278|FD-Score:5.17|P-value:1.17E-7 ID:SGTC_1355|Compound:1499-0035|FD-Score:4.21|P-value:1.25E-5 ID:SGTC_1527|Compound:coumarin|FD-Score:-3.45|P-value:2.57E-4 ID:SGTC_1634|Compound:st006576|FD-Score:3.53|P-value:2.13E-4 ID:SGTC_1824|Compound:st053723|FD-Score:3.28|P-value:5.21E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:-3.56|P-value:1.68E-4 ID:SGTC_1962|Compound:st076595|FD-Score:8.68|P-value:1.82E-18 ID:SGTC_1975|Compound:st070967|FD-Score:-4.90|P-value:4.11E-7 ID:SGTC_2013|Compound:4024628|FD-Score:3.28|P-value:5.29E-4 ID:SGTC_2025|Compound:5147754|FD-Score:3.95|P-value:4.00E-5 ID:SGTC_2054|Compound:5233173|FD-Score:3.37|P-value:3.80E-4 ID:SGTC_2282|Compound:7944268|FD-Score:-3.25|P-value:5.29E-4 ID:SGTC_2284|Compound:7945075|FD-Score:4.64|P-value:1.70E-6 ID:SGTC_2322|Compound:7413319|FD-Score:4.53|P-value:2.95E-6 ID:SGTC_2328|Compound:6201408|FD-Score:3.13|P-value:8.90E-4 ID:SGTC_2336|Compound:9001819|FD-Score:-5.31|P-value:4.55E-8 ID:SGTC_2552|Compound:juarezic acid|FD-Score:6.01|P-value:8.78E-10 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.95|P-value:3.55E-5 ID:SGTC_2895|Compound:9054447|FD-Score:3.13|P-value:8.77E-4 ID:SGTC_2969|Compound:9091408|FD-Score:-4.40|P-value:4.86E-6 ID:SGTC_2985|Compound:9022375|FD-Score:-3.30|P-value:4.52E-4 ID:SGTC_3094|Compound:9117742|FD-Score:3.21|P-value:6.65E-4 ID:SGTC_3162|Compound:9099653|FD-Score:-4.27|P-value:8.66E-6 ID:SGTC_3180|Compound:9109287|FD-Score:5.29|P-value:6.09E-8 ID:SGTC_3181|Compound:9108277|FD-Score:6.09|P-value:5.50E-10 ID:SGTC_3202|Compound:9113761|FD-Score:3.22|P-value:6.49E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:3.54|P-value:1.98E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.12|P-value:1.87E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:3.40|P-value:3.43E-4 ID:SGTC_357|Compound:1325-0123|FD-Score:-4.41|P-value:4.56E-6 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:3.73|P-value:9.64E-5 ID:SGTC_783|Compound:0848-0141|FD-Score:3.24|P-value:6.15E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:3.19|P-value:7.30E-4 ID:SGTC_1223|Compound:0764-0573|FD-Score:3.79|P-value:7.67E-5 ID:SGTC_1298|Compound:1079-1278|FD-Score:5.17|P-value:1.17E-7 ID:SGTC_1355|Compound:1499-0035|FD-Score:4.21|P-value:1.25E-5 ID:SGTC_1527|Compound:coumarin|FD-Score:-3.45|P-value:2.57E-4 ID:SGTC_1634|Compound:st006576|FD-Score:3.53|P-value:2.13E-4 ID:SGTC_1824|Compound:st053723|FD-Score:3.28|P-value:5.21E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:-3.56|P-value:1.68E-4 ID:SGTC_1962|Compound:st076595|FD-Score:8.68|P-value:1.82E-18 ID:SGTC_1975|Compound:st070967|FD-Score:-4.90|P-value:4.11E-7 ID:SGTC_2013|Compound:4024628|FD-Score:3.28|P-value:5.29E-4 ID:SGTC_2025|Compound:5147754|FD-Score:3.95|P-value:4.00E-5 ID:SGTC_2054|Compound:5233173|FD-Score:3.37|P-value:3.80E-4 ID:SGTC_2282|Compound:7944268|FD-Score:-3.25|P-value:5.29E-4 ID:SGTC_2284|Compound:7945075|FD-Score:4.64|P-value:1.70E-6 ID:SGTC_2322|Compound:7413319|FD-Score:4.53|P-value:2.95E-6 ID:SGTC_2328|Compound:6201408|FD-Score:3.13|P-value:8.90E-4 ID:SGTC_2336|Compound:9001819|FD-Score:-5.31|P-value:4.55E-8 ID:SGTC_2552|Compound:juarezic acid|FD-Score:6.01|P-value:8.78E-10 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.95|P-value:3.55E-5 ID:SGTC_2895|Compound:9054447|FD-Score:3.13|P-value:8.77E-4 ID:SGTC_2969|Compound:9091408|FD-Score:-4.40|P-value:4.86E-6 ID:SGTC_2985|Compound:9022375|FD-Score:-3.30|P-value:4.52E-4 ID:SGTC_3094|Compound:9117742|FD-Score:3.21|P-value:6.65E-4 ID:SGTC_3162|Compound:9099653|FD-Score:-4.27|P-value:8.66E-6 ID:SGTC_3180|Compound:9109287|FD-Score:5.29|P-value:6.09E-8 ID:SGTC_3181|Compound:9108277|FD-Score:6.09|P-value:5.50E-10 ID:SGTC_3202|Compound:9113761|FD-Score:3.22|P-value:6.49E-4

Top fitness defect scores for YJR047C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1962 st076595 8.68 64.5 µM 1.82E-18 redox potentiating 1 2.04E-18
2 SGTC_3181 9108277 6.09 49.5 µM 5.50E-10 iron homeostasis 1 5.68E-10
3 SGTC_2552 juarezic acid 6.01 87.0 µM 8.78E-10 TSC3-RPN4 7 9.06E-10
4 SGTC_3180 9109287 5.29 49.5 µM 6.09E-8 2 6.18E-8
5 SGTC_1298 1079-1278 5.17 263.0 µM 1.17E-7 DNA damage response 4 1.19E-7
6 SGTC_2284 7945075 4.64 39.2 µM 1.70E-6 mitochondrial processes 16 1.71E-6
7 SGTC_2322 7413319 4.53 38.2 µM 2.95E-6 ERAD & cell cycle 126 2.95E-6
8 SGTC_1355 1499-0035 4.21 1.7 µM 1.25E-5 13 1.25E-5
9 SGTC_58 0957-0393 4.12 9.0 µM 1.87E-5 cell wall 362 1.86E-5
10 SGTC_2025 5147754 3.95 141.0 µM 4.00E-5 8 3.98E-5
11 SGTC_1223 0764-0573 3.79 147.0 µM 7.67E-5 24 7.62E-5
12 SGTC_434 1-hexadecyl-2-methylglycero-3 PC 3.73 8.0 µM 9.64E-5 31 9.57E-5
13 SGTC_55 0767-0285 3.54 6.6 µM 1.98E-4 54 1.96E-4
14 SGTC_1634 st006576 3.53 41.0 µM 2.13E-4 TSC3-RPN4 30 2.11E-4
15 SGTC_115 0527-0192 3.40 112.0 µM 3.43E-4 103 3.39E-4
16 SGTC_2054 5233173 3.37 129.0 µM 3.80E-4 mitochondrial response to ROS 83 3.76E-4
17 SGTC_1824 st053723 3.28 68.6 µM 5.21E-4 56 5.15E-4
18 SGTC_2013 4024628 3.28 200.0 µM 5.29E-4 71 5.23E-4
19 SGTC_783 0848-0141 3.24 92.7 µM 6.15E-4 RSC & ERG11 31 6.08E-4
20 SGTC_3202 9113761 3.22 49.5 µM 6.49E-4 82 6.41E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.127 1.32E-13 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.118 8.01E-12 YOR179C SYC1 hom Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication
0.109 2.46E-10 YDL210W UGA4 hom Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane
0.102 2.93E-9 YML078W CPR3 hom Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria
0.101 5.21E-9 YMR094W CTF13 het Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis
0.096 2.23E-8 YDR306C_p YDR306C_p hom F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain
0.096 2.61E-8 YCR043C_p YCR043C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene
0.096 2.93E-8 YLR377C FBP1 hom Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p
0.095 3.49E-8 YNL179C_d YNL179C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance
0.094 4.54E-8 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.094 4.80E-8 YER183C FAU1 hom 5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis
0.093 6.58E-8 YBR208C DUR1,2 hom Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress
0.092 1.00E-7 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.090 1.82E-7 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.090 1.98E-7 YNR054C ESF2 het Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome