YJR050W / ISY1

Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR050W / ISY1

Click on Significant Values for Screen Details ID:SGTC_167|Compound:3672-0051|FD-Score:3.55|P-value:6.86E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:-4.25|P-value:6.61E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:-3.72|P-value:4.09E-4 ID:SGTC_444|Compound:curcumin|FD-Score:4.22|P-value:7.31E-5 ID:SGTC_450|Compound:aminopyrene|FD-Score:4.49|P-value:2.57E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.64|P-value:5.42E-4 ID:SGTC_523|Compound:1934-0222|FD-Score:5.75|P-value:1.13E-7 ID:SGTC_764|Compound:1951-2361|FD-Score:4.12|P-value:1.01E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:5.21|P-value:1.34E-6 ID:SGTC_792|Compound:4534-1957|FD-Score:3.48|P-value:8.61E-4 ID:SGTC_982|Compound:1491-0518|FD-Score:3.70|P-value:4.26E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:3.49|P-value:8.35E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:3.94|P-value:1.93E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:-4.39|P-value:3.94E-5 ID:SGTC_1199|Compound:1638-0010|FD-Score:5.25|P-value:1.13E-6 ID:SGTC_1305|Compound:1181-0477|FD-Score:-4.05|P-value:1.34E-4 ID:SGTC_1610|Compound:st001799|FD-Score:4.38|P-value:3.95E-5 ID:SGTC_1629|Compound:st005085|FD-Score:3.89|P-value:2.30E-4 ID:SGTC_1738|Compound:st035555|FD-Score:3.56|P-value:6.65E-4 ID:SGTC_1752|Compound:st044819|FD-Score:3.89|P-value:2.27E-4 ID:SGTC_1836|Compound:st055642|FD-Score:6.19|P-value:1.23E-8 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.87|P-value:2.43E-4 ID:SGTC_1911|Compound:st060208|FD-Score:5.00|P-value:3.30E-6 ID:SGTC_1914|Compound:st062367|FD-Score:3.68|P-value:4.58E-4 ID:SGTC_1937|Compound:st074714|FD-Score:4.30|P-value:5.38E-5 ID:SGTC_1990|Compound:st072050|FD-Score:4.07|P-value:1.22E-4 ID:SGTC_2049|Compound:5262831|FD-Score:-10.50|P-value:1.86E-21 ID:SGTC_2053|Compound:5265679|FD-Score:-4.43|P-value:3.39E-5 ID:SGTC_2058|Compound:5236698|FD-Score:9.66|P-value:1.69E-18 ID:SGTC_2291|Compound:7917025|FD-Score:4.53|P-value:2.22E-5 ID:SGTC_2347|Compound:9049783|FD-Score:5.93|P-value:4.54E-8 ID:SGTC_2351|Compound:9026858|FD-Score:-5.20|P-value:1.50E-6 ID:SGTC_2401|Compound:5128683|FD-Score:-3.44|P-value:9.95E-4 ID:SGTC_2475|Compound:5812728|FD-Score:3.60|P-value:5.92E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.54|P-value:7.13E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-5.54|P-value:3.14E-7 ID:SGTC_2600|Compound:tangeritin|FD-Score:-3.88|P-value:2.43E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.91|P-value:2.16E-4 ID:SGTC_2870|Compound:9038528|FD-Score:5.52|P-value:3.36E-7 ID:SGTC_2876|Compound:9059903|FD-Score:-6.01|P-value:3.33E-8 ID:SGTC_2904|Compound:7225135|FD-Score:4.38|P-value:3.91E-5 ID:SGTC_2971|Compound:9092186|FD-Score:4.46|P-value:2.96E-5 ID:SGTC_2993|Compound:9054257|FD-Score:-3.50|P-value:8.34E-4 ID:SGTC_3000|Compound:9075062|FD-Score:7.01|P-value:1.42E-10 ID:SGTC_3005|Compound:9076496|FD-Score:4.19|P-value:8.00E-5 ID:SGTC_3009|Compound:9080819|FD-Score:7.43|P-value:1.14E-11 ID:SGTC_3140|Compound:9094243|FD-Score:4.51|P-value:2.44E-5 ID:SGTC_3163|Compound:9099914|FD-Score:3.74|P-value:3.82E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:3.55|P-value:6.86E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:-4.25|P-value:6.61E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:-3.72|P-value:4.09E-4 ID:SGTC_444|Compound:curcumin|FD-Score:4.22|P-value:7.31E-5 ID:SGTC_450|Compound:aminopyrene|FD-Score:4.49|P-value:2.57E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.64|P-value:5.42E-4 ID:SGTC_523|Compound:1934-0222|FD-Score:5.75|P-value:1.13E-7 ID:SGTC_764|Compound:1951-2361|FD-Score:4.12|P-value:1.01E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:5.21|P-value:1.34E-6 ID:SGTC_792|Compound:4534-1957|FD-Score:3.48|P-value:8.61E-4 ID:SGTC_982|Compound:1491-0518|FD-Score:3.70|P-value:4.26E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:3.49|P-value:8.35E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:3.94|P-value:1.93E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:-4.39|P-value:3.94E-5 ID:SGTC_1199|Compound:1638-0010|FD-Score:5.25|P-value:1.13E-6 ID:SGTC_1305|Compound:1181-0477|FD-Score:-4.05|P-value:1.34E-4 ID:SGTC_1610|Compound:st001799|FD-Score:4.38|P-value:3.95E-5 ID:SGTC_1629|Compound:st005085|FD-Score:3.89|P-value:2.30E-4 ID:SGTC_1738|Compound:st035555|FD-Score:3.56|P-value:6.65E-4 ID:SGTC_1752|Compound:st044819|FD-Score:3.89|P-value:2.27E-4 ID:SGTC_1836|Compound:st055642|FD-Score:6.19|P-value:1.23E-8 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.87|P-value:2.43E-4 ID:SGTC_1911|Compound:st060208|FD-Score:5.00|P-value:3.30E-6 ID:SGTC_1914|Compound:st062367|FD-Score:3.68|P-value:4.58E-4 ID:SGTC_1937|Compound:st074714|FD-Score:4.30|P-value:5.38E-5 ID:SGTC_1990|Compound:st072050|FD-Score:4.07|P-value:1.22E-4 ID:SGTC_2049|Compound:5262831|FD-Score:-10.50|P-value:1.86E-21 ID:SGTC_2053|Compound:5265679|FD-Score:-4.43|P-value:3.39E-5 ID:SGTC_2058|Compound:5236698|FD-Score:9.66|P-value:1.69E-18 ID:SGTC_2291|Compound:7917025|FD-Score:4.53|P-value:2.22E-5 ID:SGTC_2347|Compound:9049783|FD-Score:5.93|P-value:4.54E-8 ID:SGTC_2351|Compound:9026858|FD-Score:-5.20|P-value:1.50E-6 ID:SGTC_2401|Compound:5128683|FD-Score:-3.44|P-value:9.95E-4 ID:SGTC_2475|Compound:5812728|FD-Score:3.60|P-value:5.92E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.54|P-value:7.13E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-5.54|P-value:3.14E-7 ID:SGTC_2600|Compound:tangeritin|FD-Score:-3.88|P-value:2.43E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.91|P-value:2.16E-4 ID:SGTC_2870|Compound:9038528|FD-Score:5.52|P-value:3.36E-7 ID:SGTC_2876|Compound:9059903|FD-Score:-6.01|P-value:3.33E-8 ID:SGTC_2904|Compound:7225135|FD-Score:4.38|P-value:3.91E-5 ID:SGTC_2971|Compound:9092186|FD-Score:4.46|P-value:2.96E-5 ID:SGTC_2993|Compound:9054257|FD-Score:-3.50|P-value:8.34E-4 ID:SGTC_3000|Compound:9075062|FD-Score:7.01|P-value:1.42E-10 ID:SGTC_3005|Compound:9076496|FD-Score:4.19|P-value:8.00E-5 ID:SGTC_3009|Compound:9080819|FD-Score:7.43|P-value:1.14E-11 ID:SGTC_3140|Compound:9094243|FD-Score:4.51|P-value:2.44E-5 ID:SGTC_3163|Compound:9099914|FD-Score:3.74|P-value:3.82E-4

Top fitness defect scores for YJR050W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2058 5236698 9.66 133.0 µM 1.69E-18 4 2.19E-22
2 SGTC_3009 9080819 7.43 71.4 µM 1.14E-11 2 5.55E-14
3 SGTC_3000 9075062 7.01 71.4 µM 1.42E-10 1 1.23E-12
4 SGTC_1836 st055642 6.19 81.0 µM 1.23E-8 3 2.96E-10
5 SGTC_2347 9049783 5.93 174.8 µM 4.54E-8 7 1.47E-9
6 SGTC_523 1934-0222 5.75 13.7 µM 1.13E-7 9 4.49E-9
7 SGTC_2870 9038528 5.52 13.0 µM 3.36E-7 4 1.71E-8
8 SGTC_1199 1638-0010 5.25 4.5 µM 1.13E-6 endomembrane recycling 4 7.58E-8
9 SGTC_782 0083-0095 5.21 30.5 µM 1.34E-6 9 9.37E-8
10 SGTC_1911 st060208 5.00 74.3 µM 3.30E-6 14 2.82E-7
11 SGTC_2291 7917025 4.53 104.3 µM 2.22E-5 iron homeostasis 36 2.91E-6
12 SGTC_3140 9094243 4.51 49.5 µM 2.44E-5 20 3.25E-6
13 SGTC_450 aminopyrene 4.49 156.0 nM 2.57E-5 20 3.48E-6
14 SGTC_2971 9092186 4.46 9.9 µM 2.96E-5 ergosterol biosynthesis 19 4.13E-6
15 SGTC_2904 7225135 4.38 42.0 µM 3.91E-5 4 5.81E-6
16 SGTC_1610 st001799 4.38 48.1 µM 3.95E-5 7 5.89E-6
17 SGTC_1937 st074714 4.30 47.2 µM 5.38E-5 32 8.56E-6
18 SGTC_444 curcumin 4.22 90.4 µM 7.31E-5 iron homeostasis 21 1.25E-5
19 SGTC_3005 9076496 4.19 71.4 µM 8.00E-5 4 1.39E-5
20 SGTC_764 1951-2361 4.12 146.0 µM 1.01E-4 31 1.86E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.182 2.62E-26 YNL176C_p TDA7_p hom Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele
0.139 6.64E-16 YNL285W_d YNL285W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.119 4.23E-12 YLR344W RPL26A hom Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication
0.108 3.34E-10 YDR371W_p CTS2_p hom Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect
0.093 7.99E-8 YOR178C GAC1 hom Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock
0.092 1.04E-7 YMR058W FET3 hom Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress
0.091 1.41E-7 YLR173W_p YLR173W_p hom Putative protein of unknown function
0.091 1.51E-7 YJR133W XPT1 hom Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine
0.088 3.01E-7 YDL015C TSC13 het Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress
0.086 7.16E-7 YGR092W DBF2 hom Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis
0.080 3.08E-6 YKR004C ECM9 het Non-essential protein of unknown function
0.080 3.43E-6 YOR233W KIN4 hom Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication
0.080 3.62E-6 YLR258W GSY2 hom Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress
0.076 1.12E-5 YNL117W MLS1 hom Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic
0.075 1.48E-5 YER068C-A_d YER068C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data