YJR069C / HAM1

Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR069C / HAM1

Click on Significant Values for Screen Details ID:SGTC_4|Compound:0960-0234|FD-Score:3.07|P-value:4.90E-4 ID:SGTC_8|Compound:0354-0001|FD-Score:3.26|P-value:2.39E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:3.91|P-value:1.39E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:3.92|P-value:1.34E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:3.02|P-value:6.06E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:2.92|P-value:8.78E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:5.49|P-value:2.00E-9 ID:SGTC_504|Compound:lovastatin|FD-Score:-3.69|P-value:4.25E-5 ID:SGTC_610|Compound:4215-0184|FD-Score:3.04|P-value:5.62E-4 ID:SGTC_718|Compound:1130-0073|FD-Score:3.18|P-value:3.26E-4 ID:SGTC_721|Compound:1187-1332|FD-Score:-3.26|P-value:2.58E-4 ID:SGTC_743|Compound:1275-0778|FD-Score:3.51|P-value:8.43E-5 ID:SGTC_837|Compound:0091-0265|FD-Score:3.97|P-value:1.05E-5 ID:SGTC_1054|Compound:3429-0080|FD-Score:3.80|P-value:2.33E-5 ID:SGTC_1246|Compound:0422-0085|FD-Score:4.11|P-value:5.37E-6 ID:SGTC_1249|Compound:0481-0015|FD-Score:-3.38|P-value:1.54E-4 ID:SGTC_1295|Compound:1013-0063|FD-Score:-3.07|P-value:5.29E-4 ID:SGTC_1296|Compound:1068-0102|FD-Score:-3.42|P-value:1.32E-4 ID:SGTC_1382|Compound:3002-2117|FD-Score:5.24|P-value:1.01E-8 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.07|P-value:4.97E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.23|P-value:2.69E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:4.66|P-value:2.89E-7 ID:SGTC_1526|Compound:st051518|FD-Score:-3.41|P-value:1.40E-4 ID:SGTC_1567|Compound:st069303|FD-Score:3.86|P-value:1.75E-5 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:3.48|P-value:9.59E-5 ID:SGTC_1636|Compound:st007707|FD-Score:2.92|P-value:8.71E-4 ID:SGTC_1792|Compound:st045773|FD-Score:4.26|P-value:2.50E-6 ID:SGTC_2121|Compound:5257820|FD-Score:3.46|P-value:1.03E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.43|P-value:1.20E-4 ID:SGTC_2366|Compound:9070441|FD-Score:3.34|P-value:1.70E-4 ID:SGTC_2460|Compound:5552655|FD-Score:-3.26|P-value:2.55E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:3.32|P-value:1.84E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.39|P-value:1.38E-4 ID:SGTC_2628|Compound:lathosterol|FD-Score:3.96|P-value:1.09E-5 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.56|P-value:6.85E-5 ID:SGTC_2780|Compound:5215298|FD-Score:3.79|P-value:2.40E-5 ID:SGTC_2842|Compound:9000675|FD-Score:-3.87|P-value:1.85E-5 ID:SGTC_3013|Compound:9080133|FD-Score:3.04|P-value:5.64E-4 ID:SGTC_3134|Compound:9127454|FD-Score:-3.56|P-value:7.50E-5 ID:SGTC_3191|Compound:9110352|FD-Score:-3.44|P-value:1.23E-4 ID:SGTC_3282|Compound:9108498|FD-Score:3.15|P-value:3.65E-4 ID:SGTC_3318|Compound:9139014|FD-Score:3.35|P-value:1.66E-4 ID:SGTC_4|Compound:0960-0234|FD-Score:3.07|P-value:4.90E-4 ID:SGTC_8|Compound:0354-0001|FD-Score:3.26|P-value:2.39E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:3.91|P-value:1.39E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:3.92|P-value:1.34E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:3.02|P-value:6.06E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:2.92|P-value:8.78E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:5.49|P-value:2.00E-9 ID:SGTC_504|Compound:lovastatin|FD-Score:-3.69|P-value:4.25E-5 ID:SGTC_610|Compound:4215-0184|FD-Score:3.04|P-value:5.62E-4 ID:SGTC_718|Compound:1130-0073|FD-Score:3.18|P-value:3.26E-4 ID:SGTC_721|Compound:1187-1332|FD-Score:-3.26|P-value:2.58E-4 ID:SGTC_743|Compound:1275-0778|FD-Score:3.51|P-value:8.43E-5 ID:SGTC_837|Compound:0091-0265|FD-Score:3.97|P-value:1.05E-5 ID:SGTC_1054|Compound:3429-0080|FD-Score:3.80|P-value:2.33E-5 ID:SGTC_1246|Compound:0422-0085|FD-Score:4.11|P-value:5.37E-6 ID:SGTC_1249|Compound:0481-0015|FD-Score:-3.38|P-value:1.54E-4 ID:SGTC_1295|Compound:1013-0063|FD-Score:-3.07|P-value:5.29E-4 ID:SGTC_1296|Compound:1068-0102|FD-Score:-3.42|P-value:1.32E-4 ID:SGTC_1382|Compound:3002-2117|FD-Score:5.24|P-value:1.01E-8 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.07|P-value:4.97E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.23|P-value:2.69E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:4.66|P-value:2.89E-7 ID:SGTC_1526|Compound:st051518|FD-Score:-3.41|P-value:1.40E-4 ID:SGTC_1567|Compound:st069303|FD-Score:3.86|P-value:1.75E-5 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:3.48|P-value:9.59E-5 ID:SGTC_1636|Compound:st007707|FD-Score:2.92|P-value:8.71E-4 ID:SGTC_1792|Compound:st045773|FD-Score:4.26|P-value:2.50E-6 ID:SGTC_2121|Compound:5257820|FD-Score:3.46|P-value:1.03E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.43|P-value:1.20E-4 ID:SGTC_2366|Compound:9070441|FD-Score:3.34|P-value:1.70E-4 ID:SGTC_2460|Compound:5552655|FD-Score:-3.26|P-value:2.55E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:3.32|P-value:1.84E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.39|P-value:1.38E-4 ID:SGTC_2628|Compound:lathosterol|FD-Score:3.96|P-value:1.09E-5 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.56|P-value:6.85E-5 ID:SGTC_2780|Compound:5215298|FD-Score:3.79|P-value:2.40E-5 ID:SGTC_2842|Compound:9000675|FD-Score:-3.87|P-value:1.85E-5 ID:SGTC_3013|Compound:9080133|FD-Score:3.04|P-value:5.64E-4 ID:SGTC_3134|Compound:9127454|FD-Score:-3.56|P-value:7.50E-5 ID:SGTC_3191|Compound:9110352|FD-Score:-3.44|P-value:1.23E-4 ID:SGTC_3282|Compound:9108498|FD-Score:3.15|P-value:3.65E-4 ID:SGTC_3318|Compound:9139014|FD-Score:3.35|P-value:1.66E-4

Top fitness defect scores for YJR069C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_266 formosanin C 5.49 406.0 nM 2.00E-9 sphingolipid biosynthesis & PDR1 36 1.98E-8
2 SGTC_1382 3002-2117 5.24 298.0 µM 1.01E-8 2 8.16E-8
3 SGTC_1493 4348-0038 4.66 111.0 µM 2.89E-7 RSC complex & mRNA processing 12 1.55E-6
4 SGTC_1792 st045773 4.26 43.6 µM 2.50E-6 18 1.03E-5
5 SGTC_1246 0422-0085 4.11 105.0 µM 5.37E-6 27 2.01E-5
6 SGTC_837 0091-0265 3.97 12.5 µM 1.05E-5 ubiquinone biosynthesis & proteasome 24 3.61E-5
7 SGTC_2628 lathosterol 3.96 100.0 µM 1.09E-5 32 3.76E-5
8 SGTC_58 0957-0393 3.92 9.0 µM 1.34E-5 cell wall 374 4.49E-5
9 SGTC_35 0133-0054 3.91 64.1 µM 1.39E-5 35 4.63E-5
10 SGTC_1567 st069303 3.86 68.4 µM 1.75E-5 mitochondrial processes 22 5.67E-5
11 SGTC_1054 3429-0080 3.80 167.0 µM 2.33E-5 31 7.29E-5
12 SGTC_2780 5215298 3.79 71.4 µM 2.40E-5 43 7.48E-5
13 SGTC_2661 dactinomycin 3.56 13.0 µM 6.85E-5 redox potentiating 35 1.88E-4
14 SGTC_743 1275-0778 3.51 158.0 µM 8.43E-5 excess fatty acid 53 2.26E-4
15 SGTC_1631 benzylvanillin 3.48 81.9 µM 9.59E-5 Golgi 41 2.53E-4
16 SGTC_2121 5257820 3.46 149.6 µM 1.03E-4 20 2.70E-4
17 SGTC_2169 5-(ethoxymethyl)quinolin-8-ol 3.43 49.5 µM 1.20E-4 39 3.07E-4
18 SGTC_2621 lithochol-11-enic acid 3.39 100.0 µM 1.38E-4 50 3.48E-4
19 SGTC_3318 9139014 3.35 38.6 µM 1.66E-4 28 4.09E-4
20 SGTC_2366 9070441 3.34 60.6 µM 1.70E-4 58 4.18E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.146 1.81E-17 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.144 5.03E-17 YDR208W MSS4 het Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation
0.139 6.56E-16 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.137 1.74E-15 YCL004W PGS1 het Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis
0.134 6.45E-15 YNL257C SIP3 hom Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication
0.129 5.30E-14 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.126 2.73E-13 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.126 3.07E-13 YEL035C_p UTR5_p het Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
0.125 3.40E-13 YDR088C SLU7 het RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.122 1.54E-12 YBR286W APE3 hom Vacuolar aminopeptidase Y, processed to mature form by Prb1p
0.121 1.82E-12 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.119 4.44E-12 YBR244W GPX2 hom Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress
0.118 6.00E-12 YMR102C_p YMR102C_p hom Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene
0.113 4.66E-11 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.111 9.68E-11 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1