HIPHOP chemogenomics database

YJR072C / NPA3

Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain

fixedexpanded
Profile for YJR072C / NPA3

Click on Significant Values for Screen Details

Top fitness defect scores for YJR072C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_1204	4049-0315	5.70	18.3 µM	1.12E-11		4	5.96E-9	1.13
2	SGTC_2949	9072542	5.65	2.2 µM	1.67E-11	TSC3-RPN4	3	7.97E-9	0.75
3	SGTC_3125	9127534	4.25	49.5 µM	2.97E-7		1	1.06E-5	0.30
4	SGTC_3132	9126706	4.08	49.5 µM	8.17E-7		9	2.22E-5	0.60
5	SGTC_533	1222-0222	3.97	6.7 µM	1.53E-6	PDR1	13	3.54E-5	0.12
6	SGTC_1970	st070122	3.63	35.8 µM	9.99E-6	TSC3-RPN4	11	1.41E-4	0.15
7	SGTC_820	1582-0060	3.57	59.9 µM	1.36E-5		13	1.78E-4	0.12
8	SGTC_2657	nystatin	3.57	74.4 µM	1.38E-5		9	1.79E-4	0.20
9	SGTC_2233	sulbentine	3.54	40.4 µM	1.60E-5		42	2.00E-4	0.04
10	SGTC_972	1319-0219	3.40	195.0 µM	3.30E-5		10	3.42E-4	0.06
11	SGTC_1969	st069479	3.36	40.1 µM	3.90E-5		5	3.87E-4	0.12
12	SGTC_165	k038-0023	3.29	64.4 µM	5.55E-5	ubiquinone biosynthesis & proteasome	29	5.02E-4	0.03
13	SGTC_1971	st069429	3.05	63.5 µM	1.68E-4	60S ribosome export	8	0.00114	0.03
14	SGTC_1728	st035614	3.04	33.8 µM	1.78E-4		16	0.00119	0.16
15	SGTC_175	3793-3120	3.01	23.7 µM	2.01E-4		15	0.00130	0.01
16	SGTC_2790	5423970	3.00	71.4 µM	2.11E-4	endomembrane recycling	31	0.00135	0.07
17	SGTC_1678	st019265	2.95	80.2 µM	2.64E-4	60S ribosome export	17	0.00160	0.11
18	SGTC_1987	st072290	2.94	61.9 µM	2.75E-4		14	0.00165	0.11
19	SGTC_2834	9001755	2.91	58.4 µM	3.07E-4		14	0.00179	0.03
20	SGTC_2598	perillyl alcohol	2.91	100.0 µM	3.15E-4		24	0.00182	0.06

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.217	3.54E-37	YDR201W	SPC19	het	Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
0.209	1.58E-34	YGR077C	PEX8	hom	Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.187	8.10E-28	YGR140W	CBF2	het	Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress
0.183	1.00E-26	YJL171C	YJL171C	hom	GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.182	2.95E-26	YDL199C_p	YDL199C_p	hom	Putative transporter, member of the sugar porter family
0.181	4.00E-26	YGR022C_d	YGR022C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.173	5.62E-24	YNL166C	BNI5	hom	Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.168	1.36E-22	YPR180W	AOS1	het	Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.165	6.61E-22	YDR521W_d	YDR521W_d	hom	Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.153	5.14E-19	YDL157C_p	YDL157C_p	hom	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.149	5.08E-18	YLR117C	CLF1	het	Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.148	5.24E-18	YER013W	PRP22	het	DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
0.146	1.76E-17	YOR159C	SME1	het	Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.145	3.09E-17	YDL176W	YDL176W	hom	Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.141	2.09E-16	YDR312W	SSF2	hom	Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication