YJR097W / JJJ3

Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR097W / JJJ3

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:6.31|P-value:6.36E-10 ID:SGTC_138|Compound:0850-0259|FD-Score:3.42|P-value:4.98E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.60|P-value:2.65E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:4.73|P-value:2.69E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:8.38|P-value:3.85E-16 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.15|P-value:3.65E-7 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.71|P-value:1.80E-4 ID:SGTC_485|Compound:niflumic acid|FD-Score:3.34|P-value:6.56E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:-3.96|P-value:6.87E-5 ID:SGTC_579|Compound:4491-1009|FD-Score:4.08|P-value:4.42E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:-3.22|P-value:9.59E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:-5.87|P-value:7.63E-9 ID:SGTC_770|Compound:0389-1121|FD-Score:-4.89|P-value:1.24E-6 ID:SGTC_771|Compound:0250-0039|FD-Score:-4.63|P-value:4.16E-6 ID:SGTC_772|Compound:k072-0172|FD-Score:-4.70|P-value:2.97E-6 ID:SGTC_796|Compound:1234-0020|FD-Score:6.82|P-value:2.73E-11 ID:SGTC_871|Compound:0574-0004|FD-Score:3.84|P-value:1.10E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:5.67|P-value:2.44E-8 ID:SGTC_901|Compound:1112-0062|FD-Score:4.63|P-value:4.28E-6 ID:SGTC_917|Compound:harmaline|FD-Score:3.22|P-value:9.69E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:3.77|P-value:1.47E-4 ID:SGTC_971|Compound:1315-0384|FD-Score:5.10|P-value:4.59E-7 ID:SGTC_977|Compound:1348-1378|FD-Score:5.04|P-value:6.17E-7 ID:SGTC_979|Compound:1426-2828|FD-Score:-3.50|P-value:3.76E-4 ID:SGTC_989|Compound:1501-0895|FD-Score:5.28|P-value:1.90E-7 ID:SGTC_1093|Compound:fluphenazine|FD-Score:3.25|P-value:8.86E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:3.53|P-value:3.41E-4 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.26|P-value:8.54E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:3.43|P-value:4.93E-4 ID:SGTC_1504|Compound:k297-0033|FD-Score:3.53|P-value:3.44E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:4.63|P-value:4.29E-6 ID:SGTC_1570|Compound:verbenone|FD-Score:-5.73|P-value:1.72E-8 ID:SGTC_1574|Compound:spiramycin|FD-Score:3.50|P-value:3.88E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:-3.48|P-value:3.98E-4 ID:SGTC_1638|Compound:st008370|FD-Score:3.51|P-value:3.77E-4 ID:SGTC_1641|Compound:st009299|FD-Score:4.74|P-value:2.64E-6 ID:SGTC_1646|Compound:st011709|FD-Score:-4.02|P-value:5.43E-5 ID:SGTC_1754|Compound:st042065|FD-Score:7.78|P-value:3.58E-14 ID:SGTC_1760|Compound:st045453|FD-Score:4.54|P-value:6.41E-6 ID:SGTC_1800|Compound:st050079|FD-Score:4.41|P-value:1.13E-5 ID:SGTC_1801|Compound:st050089|FD-Score:4.88|P-value:1.36E-6 ID:SGTC_1888|Compound:azatryptophan|FD-Score:5.45|P-value:7.91E-8 ID:SGTC_1941|Compound:st076221|FD-Score:5.23|P-value:2.50E-7 ID:SGTC_2016|Compound:5121758|FD-Score:5.01|P-value:7.34E-7 ID:SGTC_2110|Compound:5483698|FD-Score:3.27|P-value:8.32E-4 ID:SGTC_2246|Compound:7293507|FD-Score:7.07|P-value:5.30E-12 ID:SGTC_2297|Compound:7971671|FD-Score:4.33|P-value:1.54E-5 ID:SGTC_2341|Compound:7987486|FD-Score:-3.42|P-value:4.82E-4 ID:SGTC_2381|Compound:9035592|FD-Score:-3.46|P-value:4.28E-4 ID:SGTC_2384|Compound:9036245|FD-Score:-3.63|P-value:2.33E-4 ID:SGTC_2397|Compound:5161689|FD-Score:-3.28|P-value:7.90E-4 ID:SGTC_2479|Compound:5617353|FD-Score:3.43|P-value:4.82E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:3.57|P-value:3.03E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.63|P-value:2.34E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.42|P-value:5.11E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:-4.18|P-value:2.84E-5 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:4.40|P-value:1.16E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:8.75|P-value:1.93E-17 ID:SGTC_2679|Compound:rapamycin|FD-Score:7.67|P-value:8.22E-14 ID:SGTC_2683|Compound:piperine|FD-Score:3.25|P-value:8.90E-4 ID:SGTC_2823|Compound:7987624|FD-Score:4.24|P-value:2.25E-5 ID:SGTC_2858|Compound:9025781|FD-Score:5.01|P-value:7.36E-7 ID:SGTC_2953|Compound:9074162|FD-Score:4.58|P-value:5.36E-6 ID:SGTC_2962|Compound:9082311|FD-Score:3.28|P-value:8.02E-4 ID:SGTC_3011|Compound:9081274|FD-Score:4.11|P-value:3.93E-5 ID:SGTC_3037|Compound:9091026|FD-Score:4.26|P-value:2.08E-5 ID:SGTC_3114|Compound:9122766|FD-Score:3.72|P-value:1.76E-4 ID:SGTC_3141|Compound:9094609|FD-Score:-3.46|P-value:4.28E-4 ID:SGTC_3166|Compound:9102297|FD-Score:3.45|P-value:4.52E-4 ID:SGTC_3218|Compound:9131285|FD-Score:3.99|P-value:6.29E-5 ID:SGTC_3252|Compound:9135871|FD-Score:3.53|P-value:3.45E-4 ID:SGTC_3346|Compound:9158254|FD-Score:-3.74|P-value:1.59E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:6.31|P-value:6.36E-10 ID:SGTC_138|Compound:0850-0259|FD-Score:3.42|P-value:4.98E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.60|P-value:2.65E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:4.73|P-value:2.69E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:8.38|P-value:3.85E-16 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.15|P-value:3.65E-7 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.71|P-value:1.80E-4 ID:SGTC_485|Compound:niflumic acid|FD-Score:3.34|P-value:6.56E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:-3.96|P-value:6.87E-5 ID:SGTC_579|Compound:4491-1009|FD-Score:4.08|P-value:4.42E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:-3.22|P-value:9.59E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:-5.87|P-value:7.63E-9 ID:SGTC_770|Compound:0389-1121|FD-Score:-4.89|P-value:1.24E-6 ID:SGTC_771|Compound:0250-0039|FD-Score:-4.63|P-value:4.16E-6 ID:SGTC_772|Compound:k072-0172|FD-Score:-4.70|P-value:2.97E-6 ID:SGTC_796|Compound:1234-0020|FD-Score:6.82|P-value:2.73E-11 ID:SGTC_871|Compound:0574-0004|FD-Score:3.84|P-value:1.10E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:5.67|P-value:2.44E-8 ID:SGTC_901|Compound:1112-0062|FD-Score:4.63|P-value:4.28E-6 ID:SGTC_917|Compound:harmaline|FD-Score:3.22|P-value:9.69E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:3.77|P-value:1.47E-4 ID:SGTC_971|Compound:1315-0384|FD-Score:5.10|P-value:4.59E-7 ID:SGTC_977|Compound:1348-1378|FD-Score:5.04|P-value:6.17E-7 ID:SGTC_979|Compound:1426-2828|FD-Score:-3.50|P-value:3.76E-4 ID:SGTC_989|Compound:1501-0895|FD-Score:5.28|P-value:1.90E-7 ID:SGTC_1093|Compound:fluphenazine|FD-Score:3.25|P-value:8.86E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:3.53|P-value:3.41E-4 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.26|P-value:8.54E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:3.43|P-value:4.93E-4 ID:SGTC_1504|Compound:k297-0033|FD-Score:3.53|P-value:3.44E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:4.63|P-value:4.29E-6 ID:SGTC_1570|Compound:verbenone|FD-Score:-5.73|P-value:1.72E-8 ID:SGTC_1574|Compound:spiramycin|FD-Score:3.50|P-value:3.88E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:-3.48|P-value:3.98E-4 ID:SGTC_1638|Compound:st008370|FD-Score:3.51|P-value:3.77E-4 ID:SGTC_1641|Compound:st009299|FD-Score:4.74|P-value:2.64E-6 ID:SGTC_1646|Compound:st011709|FD-Score:-4.02|P-value:5.43E-5 ID:SGTC_1754|Compound:st042065|FD-Score:7.78|P-value:3.58E-14 ID:SGTC_1760|Compound:st045453|FD-Score:4.54|P-value:6.41E-6 ID:SGTC_1800|Compound:st050079|FD-Score:4.41|P-value:1.13E-5 ID:SGTC_1801|Compound:st050089|FD-Score:4.88|P-value:1.36E-6 ID:SGTC_1888|Compound:azatryptophan|FD-Score:5.45|P-value:7.91E-8 ID:SGTC_1941|Compound:st076221|FD-Score:5.23|P-value:2.50E-7 ID:SGTC_2016|Compound:5121758|FD-Score:5.01|P-value:7.34E-7 ID:SGTC_2110|Compound:5483698|FD-Score:3.27|P-value:8.32E-4 ID:SGTC_2246|Compound:7293507|FD-Score:7.07|P-value:5.30E-12 ID:SGTC_2297|Compound:7971671|FD-Score:4.33|P-value:1.54E-5 ID:SGTC_2341|Compound:7987486|FD-Score:-3.42|P-value:4.82E-4 ID:SGTC_2381|Compound:9035592|FD-Score:-3.46|P-value:4.28E-4 ID:SGTC_2384|Compound:9036245|FD-Score:-3.63|P-value:2.33E-4 ID:SGTC_2397|Compound:5161689|FD-Score:-3.28|P-value:7.90E-4 ID:SGTC_2479|Compound:5617353|FD-Score:3.43|P-value:4.82E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:3.57|P-value:3.03E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.63|P-value:2.34E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.42|P-value:5.11E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:-4.18|P-value:2.84E-5 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:4.40|P-value:1.16E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:8.75|P-value:1.93E-17 ID:SGTC_2679|Compound:rapamycin|FD-Score:7.67|P-value:8.22E-14 ID:SGTC_2683|Compound:piperine|FD-Score:3.25|P-value:8.90E-4 ID:SGTC_2823|Compound:7987624|FD-Score:4.24|P-value:2.25E-5 ID:SGTC_2858|Compound:9025781|FD-Score:5.01|P-value:7.36E-7 ID:SGTC_2953|Compound:9074162|FD-Score:4.58|P-value:5.36E-6 ID:SGTC_2962|Compound:9082311|FD-Score:3.28|P-value:8.02E-4 ID:SGTC_3011|Compound:9081274|FD-Score:4.11|P-value:3.93E-5 ID:SGTC_3037|Compound:9091026|FD-Score:4.26|P-value:2.08E-5 ID:SGTC_3114|Compound:9122766|FD-Score:3.72|P-value:1.76E-4 ID:SGTC_3141|Compound:9094609|FD-Score:-3.46|P-value:4.28E-4 ID:SGTC_3166|Compound:9102297|FD-Score:3.45|P-value:4.52E-4 ID:SGTC_3218|Compound:9131285|FD-Score:3.99|P-value:6.29E-5 ID:SGTC_3252|Compound:9135871|FD-Score:3.53|P-value:3.45E-4 ID:SGTC_3346|Compound:9158254|FD-Score:-3.74|P-value:1.59E-4

Top fitness defect scores for YJR097W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2651 1327-0036 8.75 5.0 µM 1.93E-17 32 1.08E-18
2 SGTC_221 trifluoperazine 8.38 10.3 µM 3.85E-16 NEO1-PIK1 79 2.71E-17
3 SGTC_1754 st042065 7.78 16.1 µM 3.58E-14 NEO1-PIK1 60 3.60E-15
4 SGTC_2679 rapamycin 7.67 1.0 nM 8.22E-14 45 8.83E-15
5 SGTC_2246 7293507 7.07 91.6 µM 5.30E-12 9 7.90E-13
6 SGTC_796 1234-0020 6.82 70.7 µM 2.73E-11 SWF1 & branched chain AA biosynthesis 5 4.63E-12
7 SGTC_58 0957-0393 6.31 9.0 µM 6.36E-10 cell wall 193 1.38E-10
8 SGTC_888 0916-0002 5.67 7.0 µM 2.44E-8 NEO1 25 7.04E-9
9 SGTC_1888 azatryptophan 5.45 97.5 µM 7.91E-8 5 2.50E-8
10 SGTC_989 1501-0895 5.28 10.3 µM 1.90E-7 2 6.43E-8
11 SGTC_1941 st076221 5.23 61.8 µM 2.50E-7 sphingolipid biosynthesis & PDR1 40 8.63E-8
12 SGTC_445 e6 berbamine 5.15 66.0 µM 3.65E-7 NEO1-PIK1 55 1.30E-7
13 SGTC_971 1315-0384 5.10 26.7 µM 4.59E-7 cell wall signaling 7 1.66E-7
14 SGTC_977 1348-1378 5.04 135.0 µM 6.17E-7 10 2.28E-7
15 SGTC_2016 5121758 5.01 140.0 µM 7.34E-7 redox potentiating 19 2.76E-7
16 SGTC_2858 9025781 5.01 9.0 µM 7.36E-7 sphingolipid biosynthesis & PDR1 67 2.76E-7
17 SGTC_1801 st050089 4.88 28.4 µM 1.36E-6 NEO1-PIK1 78 5.35E-7
18 SGTC_1641 st009299 4.74 47.1 µM 2.64E-6 mitochondrial processes 2 1.09E-6
19 SGTC_178 4092-0806 4.73 87.5 µM 2.69E-6 ergosterol depletion effects on membrane 61 1.12E-6
20 SGTC_901 1112-0062 4.63 60.1 µM 4.28E-6 5 1.84E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.284 2.64E-63 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.274 4.78E-59 YKL191W DPH2 hom Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
0.274 4.94E-59 YLR020C YEH2 hom Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism
0.269 1.13E-56 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.247 5.50E-48 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.240 2.70E-45 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.228 7.82E-41 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.212 1.56E-35 YML106W URA5 hom Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication
0.211 3.25E-35 YLR172C DPH5 hom Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm
0.211 5.18E-35 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.208 4.86E-34 YPL202C AFT2 hom Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication
0.205 2.46E-33 YBR245C ISW1 hom ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions
0.199 2.53E-31 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.198 4.16E-31 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.194 1.04E-29 YOR115C TRS33 hom One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic