YJR103W / URA8

Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR103W / URA8

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-3.58|P-value:3.39E-5 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.79|P-value:1.23E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:-3.08|P-value:3.05E-4 ID:SGTC_364|Compound:0083-0097|FD-Score:-3.21|P-value:1.79E-4 ID:SGTC_393|Compound:acivicin|FD-Score:12.00|P-value:2.94E-41 ID:SGTC_515|Compound:4092-1064|FD-Score:4.03|P-value:3.04E-6 ID:SGTC_620|Compound:0109-0052|FD-Score:3.89|P-value:6.55E-6 ID:SGTC_621|Compound:3584-0052|FD-Score:3.25|P-value:1.35E-4 ID:SGTC_693|Compound:4161-2696|FD-Score:3.56|P-value:3.31E-5 ID:SGTC_695|Compound:3505-6186|FD-Score:3.58|P-value:3.00E-5 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.91|P-value:6.46E-6 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.00|P-value:4.19E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:4.38|P-value:4.45E-7 ID:SGTC_873|Compound:0591-4978|FD-Score:2.96|P-value:4.39E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:4.28|P-value:8.11E-7 ID:SGTC_885|Compound:0865-0106|FD-Score:-2.90|P-value:6.23E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:3.14|P-value:2.12E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:3.18|P-value:1.80E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:3.45|P-value:5.38E-5 ID:SGTC_1303|Compound:1160-0052|FD-Score:-3.66|P-value:2.31E-5 ID:SGTC_1325|Compound:1431-0015|FD-Score:-2.83|P-value:8.20E-4 ID:SGTC_1832|Compound:st054113|FD-Score:3.15|P-value:2.05E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.99|P-value:4.40E-6 ID:SGTC_2087|Compound:5276107|FD-Score:-3.79|P-value:1.20E-5 ID:SGTC_2103|Compound:5378138|FD-Score:7.86|P-value:6.51E-19 ID:SGTC_2187|Compound:6125751|FD-Score:3.02|P-value:3.48E-4 ID:SGTC_2217|Compound:6934799|FD-Score:3.12|P-value:2.29E-4 ID:SGTC_2272|Compound:7805186|FD-Score:2.81|P-value:7.99E-4 ID:SGTC_2350|Compound:9023895|FD-Score:3.12|P-value:2.35E-4 ID:SGTC_2368|Compound:9071271|FD-Score:-3.91|P-value:6.64E-6 ID:SGTC_2400|Compound:5203494|FD-Score:-3.59|P-value:3.14E-5 ID:SGTC_2414|Compound:st077232|FD-Score:-3.07|P-value:3.17E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-2.99|P-value:4.37E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:4.51|P-value:2.20E-7 ID:SGTC_2960|Compound:9079389|FD-Score:3.65|P-value:2.08E-5 ID:SGTC_3133|Compound:9127736|FD-Score:3.02|P-value:3.55E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-3.33|P-value:1.05E-4 ID:SGTC_3342|Compound:9147791|FD-Score:2.93|P-value:5.01E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.58|P-value:3.39E-5 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.79|P-value:1.23E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:-3.08|P-value:3.05E-4 ID:SGTC_364|Compound:0083-0097|FD-Score:-3.21|P-value:1.79E-4 ID:SGTC_393|Compound:acivicin|FD-Score:12.00|P-value:2.94E-41 ID:SGTC_515|Compound:4092-1064|FD-Score:4.03|P-value:3.04E-6 ID:SGTC_620|Compound:0109-0052|FD-Score:3.89|P-value:6.55E-6 ID:SGTC_621|Compound:3584-0052|FD-Score:3.25|P-value:1.35E-4 ID:SGTC_693|Compound:4161-2696|FD-Score:3.56|P-value:3.31E-5 ID:SGTC_695|Compound:3505-6186|FD-Score:3.58|P-value:3.00E-5 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.91|P-value:6.46E-6 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.00|P-value:4.19E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:4.38|P-value:4.45E-7 ID:SGTC_873|Compound:0591-4978|FD-Score:2.96|P-value:4.39E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:4.28|P-value:8.11E-7 ID:SGTC_885|Compound:0865-0106|FD-Score:-2.90|P-value:6.23E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:3.14|P-value:2.12E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:3.18|P-value:1.80E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:3.45|P-value:5.38E-5 ID:SGTC_1303|Compound:1160-0052|FD-Score:-3.66|P-value:2.31E-5 ID:SGTC_1325|Compound:1431-0015|FD-Score:-2.83|P-value:8.20E-4 ID:SGTC_1832|Compound:st054113|FD-Score:3.15|P-value:2.05E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.99|P-value:4.40E-6 ID:SGTC_2087|Compound:5276107|FD-Score:-3.79|P-value:1.20E-5 ID:SGTC_2103|Compound:5378138|FD-Score:7.86|P-value:6.51E-19 ID:SGTC_2187|Compound:6125751|FD-Score:3.02|P-value:3.48E-4 ID:SGTC_2217|Compound:6934799|FD-Score:3.12|P-value:2.29E-4 ID:SGTC_2272|Compound:7805186|FD-Score:2.81|P-value:7.99E-4 ID:SGTC_2350|Compound:9023895|FD-Score:3.12|P-value:2.35E-4 ID:SGTC_2368|Compound:9071271|FD-Score:-3.91|P-value:6.64E-6 ID:SGTC_2400|Compound:5203494|FD-Score:-3.59|P-value:3.14E-5 ID:SGTC_2414|Compound:st077232|FD-Score:-3.07|P-value:3.17E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-2.99|P-value:4.37E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:4.51|P-value:2.20E-7 ID:SGTC_2960|Compound:9079389|FD-Score:3.65|P-value:2.08E-5 ID:SGTC_3133|Compound:9127736|FD-Score:3.02|P-value:3.55E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-3.33|P-value:1.05E-4 ID:SGTC_3342|Compound:9147791|FD-Score:2.93|P-value:5.01E-4

Top fitness defect scores for YJR103W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_393 acivicin 12.00 6.2 µM 2.94E-41 1 2.37E-33
2 SGTC_2103 5378138 7.86 10.0 µM 6.51E-19 1 1.86E-15
3 SGTC_2621 lithochol-11-enic acid 4.51 100.0 µM 2.20E-7 12 3.32E-6
4 SGTC_865 0488-0499 4.38 301.0 µM 4.45E-7 12 5.84E-6
5 SGTC_882 0843-0131 4.28 351.0 µM 8.11E-7 14 9.47E-6
6 SGTC_515 4092-1064 4.03 102.0 µM 3.04E-6 plasma membrane duress 58 2.74E-5
7 SGTC_620 0109-0052 3.89 432.0 µM 6.55E-6 22 5.10E-5
8 SGTC_2960 9079389 3.65 6.0 µM 2.08E-5 37 1.29E-4
9 SGTC_695 3505-6186 3.58 243.0 µM 3.00E-5 33 1.74E-4
10 SGTC_693 4161-2696 3.56 114.0 µM 3.31E-5 16 1.88E-4
11 SGTC_1021 4476-0760 3.45 85.3 µM 5.38E-5 46 2.78E-4
12 SGTC_621 3584-0052 3.25 32.1 µM 1.35E-4 45 5.86E-4
13 SGTC_941 3237-0776 3.18 460.0 µM 1.80E-4 51 7.38E-4
14 SGTC_1832 st054113 3.15 55.0 µM 2.05E-4 18 8.22E-4
15 SGTC_929 2805-0021 3.14 398.0 µM 2.12E-4 40 8.44E-4
16 SGTC_2217 6934799 3.12 96.1 µM 2.29E-4 TSC3-RPN4 21 8.97E-4
17 SGTC_2350 9023895 3.12 38.9 µM 2.35E-4 ergosterol biosynthesis 64 9.16E-4
18 SGTC_2187 6125751 3.02 200.0 µM 3.48E-4 RSC complex & mRNA processing 57 0.00126
19 SGTC_3133 9127736 3.02 49.5 µM 3.55E-4 57 0.00128
20 SGTC_873 0591-4978 2.96 488.0 µM 4.39E-4 52 0.00152

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.293 2.81E-67 YPR035W GLN1 het Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.157 7.08E-20 YHL023C NPR3 hom Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair
0.142 1.48E-16 YMR308C PSE1 het Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p
0.138 8.11E-16 YKL131W_d YKL131W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.117 9.92E-12 YML074C FPR3 hom Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p
0.115 2.17E-11 YPR174C YPR174C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication
0.114 3.38E-11 YEL062W NPR2 hom Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline
0.113 5.18E-11 YDR073W SNF11 hom Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions
0.103 2.05E-9 YIR019C FLO11 hom GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain
0.100 6.86E-9 YFR053C HXK1 hom Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication
0.100 7.44E-9 YLR016C PML1 hom Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
0.099 9.11E-9 YDR406W PDR15 hom Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element
0.098 1.22E-8 YMR150C IMP1 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.096 2.90E-8 YFL021W GAT1 hom Transcriptional activator of genes involved in nitrogen catabolite repression; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p
0.095 3.73E-8 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin