YJR112W / NNF1

Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJR112W / NNF1

Click on Significant Values for Screen Details ID:SGTC_29|Compound:0299-0004|FD-Score:3.43|P-value:8.23E-5 ID:SGTC_144|Compound:4161-2749|FD-Score:-5.13|P-value:7.02E-9 ID:SGTC_155|Compound:k072-0202|FD-Score:3.27|P-value:1.70E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:2.87|P-value:8.21E-4 ID:SGTC_630|Compound:4356-0227|FD-Score:-2.98|P-value:4.68E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:3.89|P-value:9.53E-6 ID:SGTC_881|Compound:0833-0569|FD-Score:-2.86|P-value:7.58E-4 ID:SGTC_890|Compound:0929-0006|FD-Score:-3.48|P-value:5.69E-5 ID:SGTC_908|Compound:3448-7491|FD-Score:-3.95|P-value:6.20E-6 ID:SGTC_954|Compound:1189-0853|FD-Score:-2.97|P-value:5.00E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:2.93|P-value:6.68E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:4.39|P-value:7.07E-7 ID:SGTC_1045|Compound:1348-1485|FD-Score:-3.06|P-value:3.39E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:-2.94|P-value:5.49E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.28|P-value:1.62E-4 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:2.93|P-value:6.54E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:2.98|P-value:5.40E-4 ID:SGTC_1216|Compound:1165-0504|FD-Score:3.25|P-value:1.85E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.25|P-value:1.83E-4 ID:SGTC_1277|Compound:0873-0036|FD-Score:3.07|P-value:3.73E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:-2.94|P-value:5.51E-4 ID:SGTC_1328|Compound:1441-0025|FD-Score:3.05|P-value:4.08E-4 ID:SGTC_1526|Compound:st051518|FD-Score:-3.24|P-value:1.66E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:2.90|P-value:7.48E-4 ID:SGTC_1674|Compound:st016616|FD-Score:3.22|P-value:2.07E-4 ID:SGTC_1682|Compound:st012968|FD-Score:-3.59|P-value:3.46E-5 ID:SGTC_1744|Compound:st037937|FD-Score:3.18|P-value:2.47E-4 ID:SGTC_2016|Compound:5121758|FD-Score:2.83|P-value:9.72E-4 ID:SGTC_2165|Compound:5727197|FD-Score:-3.03|P-value:3.94E-4 ID:SGTC_2168|Compound:5727979|FD-Score:-3.28|P-value:1.37E-4 ID:SGTC_2173|Compound:5763948|FD-Score:-2.90|P-value:6.36E-4 ID:SGTC_2181|Compound:6316240|FD-Score:2.91|P-value:7.06E-4 ID:SGTC_2182|Compound:6325192|FD-Score:4.86|P-value:4.52E-8 ID:SGTC_2265|Compound:7929201|FD-Score:-3.50|P-value:5.35E-5 ID:SGTC_2268|Compound:7933382|FD-Score:-4.03|P-value:4.00E-6 ID:SGTC_2312|Compound:7706453|FD-Score:3.99|P-value:5.98E-6 ID:SGTC_2327|Compound:6189656|FD-Score:4.33|P-value:9.83E-7 ID:SGTC_2328|Compound:6201408|FD-Score:4.07|P-value:3.99E-6 ID:SGTC_2360|Compound:9040895|FD-Score:7.20|P-value:1.15E-15 ID:SGTC_2365|Compound:9042475|FD-Score:-4.60|P-value:1.72E-7 ID:SGTC_2394|Compound:5221648|FD-Score:-3.44|P-value:6.76E-5 ID:SGTC_2398|Compound:5228213|FD-Score:5.02|P-value:1.76E-8 ID:SGTC_2421|Compound:5690543|FD-Score:2.91|P-value:7.14E-4 ID:SGTC_2479|Compound:5617353|FD-Score:3.20|P-value:2.22E-4 ID:SGTC_2484|Compound:5809818|FD-Score:-3.21|P-value:1.89E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:4.06|P-value:4.17E-6 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.34|P-value:1.25E-4 ID:SGTC_2608|Compound:thymoquinone|FD-Score:3.68|P-value:2.73E-5 ID:SGTC_2664|Compound:lyso-paf C-16|FD-Score:2.91|P-value:7.08E-4 ID:SGTC_2724|Compound:maprotiline|FD-Score:2.83|P-value:9.69E-4 ID:SGTC_2764|Compound:methyldopa|FD-Score:2.90|P-value:7.49E-4 ID:SGTC_2817|Compound:7995342|FD-Score:-3.27|P-value:1.42E-4 ID:SGTC_2826|Compound:7992541|FD-Score:-3.56|P-value:3.99E-5 ID:SGTC_2946|Compound:9065502|FD-Score:2.87|P-value:8.40E-4 ID:SGTC_3125|Compound:9127534|FD-Score:3.59|P-value:4.15E-5 ID:SGTC_3133|Compound:9127736|FD-Score:-3.25|P-value:1.54E-4 ID:SGTC_29|Compound:0299-0004|FD-Score:3.43|P-value:8.23E-5 ID:SGTC_144|Compound:4161-2749|FD-Score:-5.13|P-value:7.02E-9 ID:SGTC_155|Compound:k072-0202|FD-Score:3.27|P-value:1.70E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:2.87|P-value:8.21E-4 ID:SGTC_630|Compound:4356-0227|FD-Score:-2.98|P-value:4.68E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:3.89|P-value:9.53E-6 ID:SGTC_881|Compound:0833-0569|FD-Score:-2.86|P-value:7.58E-4 ID:SGTC_890|Compound:0929-0006|FD-Score:-3.48|P-value:5.69E-5 ID:SGTC_908|Compound:3448-7491|FD-Score:-3.95|P-value:6.20E-6 ID:SGTC_954|Compound:1189-0853|FD-Score:-2.97|P-value:5.00E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:2.93|P-value:6.68E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:4.39|P-value:7.07E-7 ID:SGTC_1045|Compound:1348-1485|FD-Score:-3.06|P-value:3.39E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:-2.94|P-value:5.49E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.28|P-value:1.62E-4 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:2.93|P-value:6.54E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:2.98|P-value:5.40E-4 ID:SGTC_1216|Compound:1165-0504|FD-Score:3.25|P-value:1.85E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.25|P-value:1.83E-4 ID:SGTC_1277|Compound:0873-0036|FD-Score:3.07|P-value:3.73E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:-2.94|P-value:5.51E-4 ID:SGTC_1328|Compound:1441-0025|FD-Score:3.05|P-value:4.08E-4 ID:SGTC_1526|Compound:st051518|FD-Score:-3.24|P-value:1.66E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:2.90|P-value:7.48E-4 ID:SGTC_1674|Compound:st016616|FD-Score:3.22|P-value:2.07E-4 ID:SGTC_1682|Compound:st012968|FD-Score:-3.59|P-value:3.46E-5 ID:SGTC_1744|Compound:st037937|FD-Score:3.18|P-value:2.47E-4 ID:SGTC_2016|Compound:5121758|FD-Score:2.83|P-value:9.72E-4 ID:SGTC_2165|Compound:5727197|FD-Score:-3.03|P-value:3.94E-4 ID:SGTC_2168|Compound:5727979|FD-Score:-3.28|P-value:1.37E-4 ID:SGTC_2173|Compound:5763948|FD-Score:-2.90|P-value:6.36E-4 ID:SGTC_2181|Compound:6316240|FD-Score:2.91|P-value:7.06E-4 ID:SGTC_2182|Compound:6325192|FD-Score:4.86|P-value:4.52E-8 ID:SGTC_2265|Compound:7929201|FD-Score:-3.50|P-value:5.35E-5 ID:SGTC_2268|Compound:7933382|FD-Score:-4.03|P-value:4.00E-6 ID:SGTC_2312|Compound:7706453|FD-Score:3.99|P-value:5.98E-6 ID:SGTC_2327|Compound:6189656|FD-Score:4.33|P-value:9.83E-7 ID:SGTC_2328|Compound:6201408|FD-Score:4.07|P-value:3.99E-6 ID:SGTC_2360|Compound:9040895|FD-Score:7.20|P-value:1.15E-15 ID:SGTC_2365|Compound:9042475|FD-Score:-4.60|P-value:1.72E-7 ID:SGTC_2394|Compound:5221648|FD-Score:-3.44|P-value:6.76E-5 ID:SGTC_2398|Compound:5228213|FD-Score:5.02|P-value:1.76E-8 ID:SGTC_2421|Compound:5690543|FD-Score:2.91|P-value:7.14E-4 ID:SGTC_2479|Compound:5617353|FD-Score:3.20|P-value:2.22E-4 ID:SGTC_2484|Compound:5809818|FD-Score:-3.21|P-value:1.89E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:4.06|P-value:4.17E-6 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.34|P-value:1.25E-4 ID:SGTC_2608|Compound:thymoquinone|FD-Score:3.68|P-value:2.73E-5 ID:SGTC_2664|Compound:lyso-paf C-16|FD-Score:2.91|P-value:7.08E-4 ID:SGTC_2724|Compound:maprotiline|FD-Score:2.83|P-value:9.69E-4 ID:SGTC_2764|Compound:methyldopa|FD-Score:2.90|P-value:7.49E-4 ID:SGTC_2817|Compound:7995342|FD-Score:-3.27|P-value:1.42E-4 ID:SGTC_2826|Compound:7992541|FD-Score:-3.56|P-value:3.99E-5 ID:SGTC_2946|Compound:9065502|FD-Score:2.87|P-value:8.40E-4 ID:SGTC_3125|Compound:9127534|FD-Score:3.59|P-value:4.15E-5 ID:SGTC_3133|Compound:9127736|FD-Score:-3.25|P-value:1.54E-4

Top fitness defect scores for YJR112W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2360 9040895 7.20 200.0 µM 1.15E-15 RPP1 & pyrimidine depletion 2 2.97E-13 0.48
2 SGTC_2398 5228213 5.02 48.8 µM 1.76E-8 iron homeostasis 8 2.64E-7 0.03
3 SGTC_2182 6325192 4.86 200.0 µM 4.52E-8 Golgi 1 5.78E-7 1.13
4 SGTC_969 1313-0238 4.39 134.0 µM 7.07E-7 mitochondrial processes 16 5.67E-6 0.02
5 SGTC_2327 6189656 4.33 200.0 µM 9.83E-7 6 7.46E-6 0.08
6 SGTC_2328 6201408 4.07 200.0 µM 3.99E-6 10 2.39E-5 0.17
7 SGTC_2566 crystal violet carbinol 4.06 226.0 nM 4.17E-6 17 2.48E-5 0.12
8 SGTC_2312 7706453 3.99 155.7 µM 5.98E-6 10 3.35E-5 0.01
9 SGTC_873 0591-4978 3.89 488.0 µM 9.53E-6 4 4.94E-5 0.24
10 SGTC_2608 thymoquinone 3.68 42.7 µM 2.73E-5 3 1.19E-4 0.06
11 SGTC_3125 9127534 3.59 49.5 µM 4.15E-5 6 1.68E-4 0.30
12 SGTC_29 0299-0004 3.43 13.0 µM 8.23E-5 3 2.98E-4 0.23
13 SGTC_2567 aureobasidin a 3.34 60.0 nM 1.25E-4 55 4.22E-4 0.06
14 SGTC_1053 3366-3204 3.28 134.0 µM 1.62E-4 cell wall 15 5.25E-4 0.07
15 SGTC_155 k072-0202 3.27 43.6 µM 1.70E-4 13 5.46E-4 0.01
16 SGTC_1246 0422-0085 3.25 105.0 µM 1.83E-4 17 5.80E-4 0.02
17 SGTC_1216 1165-0504 3.25 9.7 µM 1.85E-4 8 5.87E-4 0.01
18 SGTC_1674 st016616 3.22 23.6 µM 2.07E-4 14 6.45E-4 0.01
19 SGTC_2479 5617353 3.20 43.9 µM 2.22E-4 19 6.82E-4 0.04
20 SGTC_1744 st037937 3.18 38.9 µM 2.47E-4 11 7.46E-4 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.132 1.34E-14 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.112 6.57E-11 YHR086W NAM8 hom RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function
0.108 3.06E-10 YLL036C PRP19 het Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
0.106 8.67E-10 YNL311C SKP2 hom F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments
0.101 3.98E-9 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.100 6.80E-9 YFR027W ECO1 het Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
0.098 1.23E-8 YDR504C SPG3 hom Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
0.093 6.48E-8 YDR398W UTP5 het Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
0.093 7.07E-8 YLR127C APC2 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
0.091 1.12E-7 YDL164C CDC9 het DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination
0.089 2.53E-7 YDR344C_d YDR344C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.088 3.38E-7 YPL224C MMT2 hom Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p
0.086 6.60E-7 YBR236C ABD1 het Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA
0.084 1.24E-6 YDL227C HO hom Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p
0.081 2.45E-6 YAL034W-A MTW1 het Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly