YKL001C / MET14

Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL001C / MET14

Click on Significant Values for Screen Details ID:SGTC_23|Compound:0213-0011|FD-Score:3.42|P-value:1.42E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:2.95|P-value:8.54E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:3.62|P-value:6.24E-5 ID:SGTC_133|Compound:0724-0033|FD-Score:3.06|P-value:5.77E-4 ID:SGTC_323|Compound:4013-0906|FD-Score:3.24|P-value:2.88E-4 ID:SGTC_603|Compound:3794-0023|FD-Score:-2.95|P-value:9.73E-4 ID:SGTC_630|Compound:4356-0227|FD-Score:-3.24|P-value:3.34E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:-3.15|P-value:4.63E-4 ID:SGTC_730|Compound:1436-0052|FD-Score:3.21|P-value:3.30E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.73|P-value:4.41E-5 ID:SGTC_780|Compound:0109-0199|FD-Score:3.73|P-value:3.75E-5 ID:SGTC_886|Compound:0866-0150|FD-Score:2.99|P-value:7.38E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.21|P-value:3.67E-4 ID:SGTC_1186|Compound:1319-0216|FD-Score:3.19|P-value:3.61E-4 ID:SGTC_1216|Compound:1165-0504|FD-Score:3.16|P-value:3.94E-4 ID:SGTC_1691|Compound:st019933|FD-Score:3.28|P-value:2.50E-4 ID:SGTC_1717|Compound:st033143|FD-Score:2.93|P-value:9.38E-4 ID:SGTC_1727|Compound:st034309|FD-Score:6.43|P-value:5.10E-12 ID:SGTC_1732|Compound:st034308|FD-Score:-6.15|P-value:4.48E-11 ID:SGTC_1734|Compound:st037168|FD-Score:-4.69|P-value:3.93E-7 ID:SGTC_1736|Compound:st036774|FD-Score:5.97|P-value:1.31E-10 ID:SGTC_1923|Compound:st058458|FD-Score:2.91|P-value:0.00100 ID:SGTC_1943|Compound:st076331|FD-Score:-3.44|P-value:1.50E-4 ID:SGTC_1947|Compound:st076332|FD-Score:-3.27|P-value:2.91E-4 ID:SGTC_1991|Compound:st073000|FD-Score:3.13|P-value:4.49E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:5.40|P-value:5.17E-9 ID:SGTC_2176|Compound:5809774|FD-Score:3.26|P-value:2.73E-4 ID:SGTC_2318|Compound:6029776|FD-Score:3.37|P-value:1.72E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:3.79|P-value:2.97E-5 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.83|P-value:2.42E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.40|P-value:1.59E-6 ID:SGTC_2933|Compound:9026794|FD-Score:3.02|P-value:6.84E-4 ID:SGTC_3336|Compound:9143619|FD-Score:3.18|P-value:3.73E-4 ID:SGTC_23|Compound:0213-0011|FD-Score:3.42|P-value:1.42E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:2.95|P-value:8.54E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:3.62|P-value:6.24E-5 ID:SGTC_133|Compound:0724-0033|FD-Score:3.06|P-value:5.77E-4 ID:SGTC_323|Compound:4013-0906|FD-Score:3.24|P-value:2.88E-4 ID:SGTC_603|Compound:3794-0023|FD-Score:-2.95|P-value:9.73E-4 ID:SGTC_630|Compound:4356-0227|FD-Score:-3.24|P-value:3.34E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:-3.15|P-value:4.63E-4 ID:SGTC_730|Compound:1436-0052|FD-Score:3.21|P-value:3.30E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.73|P-value:4.41E-5 ID:SGTC_780|Compound:0109-0199|FD-Score:3.73|P-value:3.75E-5 ID:SGTC_886|Compound:0866-0150|FD-Score:2.99|P-value:7.38E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.21|P-value:3.67E-4 ID:SGTC_1186|Compound:1319-0216|FD-Score:3.19|P-value:3.61E-4 ID:SGTC_1216|Compound:1165-0504|FD-Score:3.16|P-value:3.94E-4 ID:SGTC_1691|Compound:st019933|FD-Score:3.28|P-value:2.50E-4 ID:SGTC_1717|Compound:st033143|FD-Score:2.93|P-value:9.38E-4 ID:SGTC_1727|Compound:st034309|FD-Score:6.43|P-value:5.10E-12 ID:SGTC_1732|Compound:st034308|FD-Score:-6.15|P-value:4.48E-11 ID:SGTC_1734|Compound:st037168|FD-Score:-4.69|P-value:3.93E-7 ID:SGTC_1736|Compound:st036774|FD-Score:5.97|P-value:1.31E-10 ID:SGTC_1923|Compound:st058458|FD-Score:2.91|P-value:0.00100 ID:SGTC_1943|Compound:st076331|FD-Score:-3.44|P-value:1.50E-4 ID:SGTC_1947|Compound:st076332|FD-Score:-3.27|P-value:2.91E-4 ID:SGTC_1991|Compound:st073000|FD-Score:3.13|P-value:4.49E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:5.40|P-value:5.17E-9 ID:SGTC_2176|Compound:5809774|FD-Score:3.26|P-value:2.73E-4 ID:SGTC_2318|Compound:6029776|FD-Score:3.37|P-value:1.72E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:3.79|P-value:2.97E-5 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.83|P-value:2.42E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.40|P-value:1.59E-6 ID:SGTC_2933|Compound:9026794|FD-Score:3.02|P-value:6.84E-4 ID:SGTC_3336|Compound:9143619|FD-Score:3.18|P-value:3.73E-4

Top fitness defect scores for YKL001C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1727 st034309 6.43 9.9 µM 5.10E-12 5 6.54E-11
2 SGTC_1736 st036774 5.97 51.0 µM 1.31E-10 7 1.20E-9
3 SGTC_2130 NSC 7 5.40 2.5 µM 5.17E-9 copper-dependent oxidative stress 8 3.24E-8
4 SGTC_2640 pentamidine 4.40 10.4 µM 1.59E-6 mitochondrial processes 21 5.53E-6
5 SGTC_2639 lactacystin 3.83 53.0 µM 2.42E-5 26 6.38E-5
6 SGTC_2605 digitonin 3.79 300.0 nM 2.97E-5 35 7.65E-5
7 SGTC_780 0109-0199 3.73 192.0 µM 3.75E-5 21 9.44E-5
8 SGTC_73 0327-0325 3.62 16.2 µM 6.24E-5 DNA damage response 63 1.49E-4
9 SGTC_23 0213-0011 3.42 40.0 µM 1.42E-4 copper-dependent oxidative stress 39 3.13E-4
10 SGTC_2318 6029776 3.37 164.0 µM 1.72E-4 55 3.71E-4
11 SGTC_1691 st019933 3.28 84.6 µM 2.50E-4 33 5.19E-4
12 SGTC_2176 5809774 3.26 34.0 µM 2.73E-4 mitochondrial processes 34 5.61E-4
13 SGTC_323 4013-0906 3.24 34.3 µM 2.88E-4 ERG2 24 5.88E-4
14 SGTC_730 1436-0052 3.21 41.2 µM 3.30E-4 mitochondrial processes 31 6.67E-4
15 SGTC_1186 1319-0216 3.19 26.0 µM 3.61E-4 47 7.22E-4
16 SGTC_3336 9143619 3.18 71.4 µM 3.73E-4 48 7.43E-4
17 SGTC_1216 1165-0504 3.16 9.7 µM 3.94E-4 41 7.81E-4
18 SGTC_1991 st073000 3.13 8.9 µM 4.49E-4 NEO1 120 8.78E-4
19 SGTC_133 0724-0033 3.06 378.9 µM 5.77E-4 60 0.00110
20 SGTC_2933 9026794 3.02 32.5 µM 6.84E-4 76 0.00128

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.224 2.10E-39 YLR223C IFH1 het Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
0.215 2.52E-36 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.209 2.26E-34 YBR207W FTH1 hom Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.195 3.08E-30 YOR010C TIR2 hom Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis
0.194 7.27E-30 YFL052W_p YFL052W_p hom Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity
0.188 4.24E-28 YPR010C RPA135 het RNA polymerase I second largest subunit A135
0.183 1.12E-26 YOL141W PPM2 hom AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
0.164 1.59E-21 YEL004W YEA4 hom Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER
0.160 1.07E-20 YLL046C RNP1 hom Ribonucleoprotein that contains two RNA recognition motifs (RRM)
0.154 3.41E-19 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.153 4.51E-19 YKL140W TGL1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.149 4.42E-18 YDR208W MSS4 het Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation
0.148 5.36E-18 YPL152W-A_p YPL152W-A_p hom Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.147 1.23E-17 YOR306C MCH5 hom Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport
0.145 3.56E-17 YKL072W STB6 hom Protein that binds Sin3p in a two-hybrid assay