YKL002W / DID4

Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL002W / DID4

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:5.89|P-value:2.18E-8 ID:SGTC_40|Compound:0327-0103|FD-Score:-5.25|P-value:6.74E-7 ID:SGTC_108|Compound:0509-0257|FD-Score:4.64|P-value:8.07E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:4.77|P-value:4.66E-6 ID:SGTC_124|Compound:0136-0217|FD-Score:4.16|P-value:5.32E-5 ID:SGTC_149|Compound:3970-0822|FD-Score:4.26|P-value:3.70E-5 ID:SGTC_150|Compound:3970-0969|FD-Score:3.39|P-value:8.01E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:4.27|P-value:3.59E-5 ID:SGTC_214|Compound:4373-3496|FD-Score:5.04|P-value:1.42E-6 ID:SGTC_255|Compound:tunicamycin|FD-Score:4.81|P-value:3.87E-6 ID:SGTC_263|Compound:alizarin|FD-Score:4.12|P-value:6.17E-5 ID:SGTC_268|Compound:camptothecin|FD-Score:4.11|P-value:6.52E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.13|P-value:1.18E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:3.99|P-value:1.00E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:5.93|P-value:1.79E-8 ID:SGTC_769|Compound:0302-0231|FD-Score:-3.79|P-value:2.51E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.39|P-value:7.88E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:-4.81|P-value:4.68E-6 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.51|P-value:5.41E-4 ID:SGTC_1144|Compound:3486-0183|FD-Score:4.04|P-value:8.56E-5 ID:SGTC_1194|Compound:1488-0194|FD-Score:4.43|P-value:1.85E-5 ID:SGTC_1245|Compound:0418-0076|FD-Score:-3.68|P-value:3.66E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:-3.46|P-value:7.49E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.42|P-value:7.22E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:4.83|P-value:3.52E-6 ID:SGTC_1333|Compound:1482-0047|FD-Score:5.39|P-value:2.73E-7 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:8.60|P-value:7.52E-16 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:3.34|P-value:9.25E-4 ID:SGTC_1652|Compound:st011932|FD-Score:-3.52|P-value:6.20E-4 ID:SGTC_1658|Compound:st012944|FD-Score:7.51|P-value:1.61E-12 ID:SGTC_1687|Compound:st020778|FD-Score:3.66|P-value:3.22E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.58|P-value:4.30E-4 ID:SGTC_1744|Compound:st037937|FD-Score:-3.66|P-value:3.86E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-4.29|P-value:4.04E-5 ID:SGTC_1750|Compound:st081859|FD-Score:7.16|P-value:1.45E-11 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.14|P-value:5.90E-5 ID:SGTC_1917|Compound:st066507|FD-Score:-3.37|P-value:9.98E-4 ID:SGTC_1980|Compound:st071811|FD-Score:3.82|P-value:1.88E-4 ID:SGTC_2023|Compound:5144124|FD-Score:3.79|P-value:2.08E-4 ID:SGTC_2024|Compound:5144227|FD-Score:6.35|P-value:1.81E-9 ID:SGTC_2060|Compound:5238662|FD-Score:3.94|P-value:1.21E-4 ID:SGTC_2119|Compound:5528445|FD-Score:3.79|P-value:2.09E-4 ID:SGTC_2128|Compound:5255449|FD-Score:4.51|P-value:1.35E-5 ID:SGTC_2146|Compound:5545642|FD-Score:3.41|P-value:7.35E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-5.95|P-value:2.08E-8 ID:SGTC_2267|Compound:7931562|FD-Score:-5.59|P-value:1.29E-7 ID:SGTC_2273|Compound:7815444|FD-Score:-4.85|P-value:4.10E-6 ID:SGTC_2274|Compound:7947094|FD-Score:-5.07|P-value:1.53E-6 ID:SGTC_2292|Compound:7671323|FD-Score:3.65|P-value:3.34E-4 ID:SGTC_2296|Compound:7971656|FD-Score:5.25|P-value:5.25E-7 ID:SGTC_2302|Compound:7771753|FD-Score:4.67|P-value:7.12E-6 ID:SGTC_2347|Compound:9049783|FD-Score:4.06|P-value:7.71E-5 ID:SGTC_2360|Compound:9040895|FD-Score:4.72|P-value:5.60E-6 ID:SGTC_2415|Compound:st077333|FD-Score:4.73|P-value:5.43E-6 ID:SGTC_2441|Compound:5730946|FD-Score:3.43|P-value:6.98E-4 ID:SGTC_2534|Compound:helenine|FD-Score:3.67|P-value:3.19E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:3.47|P-value:6.09E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.94|P-value:1.20E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:4.45|P-value:1.70E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-3.72|P-value:3.19E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:4.09|P-value:7.01E-5 ID:SGTC_2666|Compound:tannic acid|FD-Score:5.20|P-value:6.76E-7 ID:SGTC_2674|Compound:ligustilide|FD-Score:4.14|P-value:5.82E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.05|P-value:1.35E-6 ID:SGTC_2737|Compound:bleomycin|FD-Score:4.99|P-value:1.72E-6 ID:SGTC_2773|Compound:thioridazine|FD-Score:3.42|P-value:7.07E-4 ID:SGTC_2832|Compound:7992762|FD-Score:-4.48|P-value:1.87E-5 ID:SGTC_2887|Compound:9044784|FD-Score:4.43|P-value:1.87E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-5.51|P-value:1.98E-7 ID:SGTC_2934|Compound:9036370|FD-Score:4.22|P-value:4.31E-5 ID:SGTC_2937|Compound:9044753|FD-Score:3.78|P-value:2.12E-4 ID:SGTC_3157|Compound:9099274|FD-Score:4.63|P-value:8.26E-6 ID:SGTC_3232|Compound:9132874|FD-Score:3.91|P-value:1.36E-4 ID:SGTC_3235|Compound:9132602|FD-Score:-4.62|P-value:1.07E-5 ID:SGTC_37|Compound:0566-0070|FD-Score:5.89|P-value:2.18E-8 ID:SGTC_40|Compound:0327-0103|FD-Score:-5.25|P-value:6.74E-7 ID:SGTC_108|Compound:0509-0257|FD-Score:4.64|P-value:8.07E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:4.77|P-value:4.66E-6 ID:SGTC_124|Compound:0136-0217|FD-Score:4.16|P-value:5.32E-5 ID:SGTC_149|Compound:3970-0822|FD-Score:4.26|P-value:3.70E-5 ID:SGTC_150|Compound:3970-0969|FD-Score:3.39|P-value:8.01E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:4.27|P-value:3.59E-5 ID:SGTC_214|Compound:4373-3496|FD-Score:5.04|P-value:1.42E-6 ID:SGTC_255|Compound:tunicamycin|FD-Score:4.81|P-value:3.87E-6 ID:SGTC_263|Compound:alizarin|FD-Score:4.12|P-value:6.17E-5 ID:SGTC_268|Compound:camptothecin|FD-Score:4.11|P-value:6.52E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.13|P-value:1.18E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:3.99|P-value:1.00E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:5.93|P-value:1.79E-8 ID:SGTC_769|Compound:0302-0231|FD-Score:-3.79|P-value:2.51E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.39|P-value:7.88E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:-4.81|P-value:4.68E-6 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.51|P-value:5.41E-4 ID:SGTC_1144|Compound:3486-0183|FD-Score:4.04|P-value:8.56E-5 ID:SGTC_1194|Compound:1488-0194|FD-Score:4.43|P-value:1.85E-5 ID:SGTC_1245|Compound:0418-0076|FD-Score:-3.68|P-value:3.66E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:-3.46|P-value:7.49E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.42|P-value:7.22E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:4.83|P-value:3.52E-6 ID:SGTC_1333|Compound:1482-0047|FD-Score:5.39|P-value:2.73E-7 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:8.60|P-value:7.52E-16 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:3.34|P-value:9.25E-4 ID:SGTC_1652|Compound:st011932|FD-Score:-3.52|P-value:6.20E-4 ID:SGTC_1658|Compound:st012944|FD-Score:7.51|P-value:1.61E-12 ID:SGTC_1687|Compound:st020778|FD-Score:3.66|P-value:3.22E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.58|P-value:4.30E-4 ID:SGTC_1744|Compound:st037937|FD-Score:-3.66|P-value:3.86E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-4.29|P-value:4.04E-5 ID:SGTC_1750|Compound:st081859|FD-Score:7.16|P-value:1.45E-11 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.14|P-value:5.90E-5 ID:SGTC_1917|Compound:st066507|FD-Score:-3.37|P-value:9.98E-4 ID:SGTC_1980|Compound:st071811|FD-Score:3.82|P-value:1.88E-4 ID:SGTC_2023|Compound:5144124|FD-Score:3.79|P-value:2.08E-4 ID:SGTC_2024|Compound:5144227|FD-Score:6.35|P-value:1.81E-9 ID:SGTC_2060|Compound:5238662|FD-Score:3.94|P-value:1.21E-4 ID:SGTC_2119|Compound:5528445|FD-Score:3.79|P-value:2.09E-4 ID:SGTC_2128|Compound:5255449|FD-Score:4.51|P-value:1.35E-5 ID:SGTC_2146|Compound:5545642|FD-Score:3.41|P-value:7.35E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-5.95|P-value:2.08E-8 ID:SGTC_2267|Compound:7931562|FD-Score:-5.59|P-value:1.29E-7 ID:SGTC_2273|Compound:7815444|FD-Score:-4.85|P-value:4.10E-6 ID:SGTC_2274|Compound:7947094|FD-Score:-5.07|P-value:1.53E-6 ID:SGTC_2292|Compound:7671323|FD-Score:3.65|P-value:3.34E-4 ID:SGTC_2296|Compound:7971656|FD-Score:5.25|P-value:5.25E-7 ID:SGTC_2302|Compound:7771753|FD-Score:4.67|P-value:7.12E-6 ID:SGTC_2347|Compound:9049783|FD-Score:4.06|P-value:7.71E-5 ID:SGTC_2360|Compound:9040895|FD-Score:4.72|P-value:5.60E-6 ID:SGTC_2415|Compound:st077333|FD-Score:4.73|P-value:5.43E-6 ID:SGTC_2441|Compound:5730946|FD-Score:3.43|P-value:6.98E-4 ID:SGTC_2534|Compound:helenine|FD-Score:3.67|P-value:3.19E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:3.47|P-value:6.09E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.94|P-value:1.20E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:4.45|P-value:1.70E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-3.72|P-value:3.19E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:4.09|P-value:7.01E-5 ID:SGTC_2666|Compound:tannic acid|FD-Score:5.20|P-value:6.76E-7 ID:SGTC_2674|Compound:ligustilide|FD-Score:4.14|P-value:5.82E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.05|P-value:1.35E-6 ID:SGTC_2737|Compound:bleomycin|FD-Score:4.99|P-value:1.72E-6 ID:SGTC_2773|Compound:thioridazine|FD-Score:3.42|P-value:7.07E-4 ID:SGTC_2832|Compound:7992762|FD-Score:-4.48|P-value:1.87E-5 ID:SGTC_2887|Compound:9044784|FD-Score:4.43|P-value:1.87E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-5.51|P-value:1.98E-7 ID:SGTC_2934|Compound:9036370|FD-Score:4.22|P-value:4.31E-5 ID:SGTC_2937|Compound:9044753|FD-Score:3.78|P-value:2.12E-4 ID:SGTC_3157|Compound:9099274|FD-Score:4.63|P-value:8.26E-6 ID:SGTC_3232|Compound:9132874|FD-Score:3.91|P-value:1.36E-4 ID:SGTC_3235|Compound:9132602|FD-Score:-4.62|P-value:1.07E-5

Top fitness defect scores for YKL002W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1593 justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 8.60 54.9 µM 7.52E-16 redox potentiating 17 4.12E-18
2 SGTC_1658 st012944 7.51 20.0 µM 1.61E-12 iron homeostasis 12 3.05E-14
3 SGTC_1750 st081859 7.16 2.4 µM 1.45E-11 endomembrane recycling 85 3.92E-13
4 SGTC_2024 5144227 6.35 134.0 µM 1.81E-9 9 1.06E-10
5 SGTC_748 0136-0228 5.93 77.9 µM 1.79E-8 redox potentiating 38 1.51E-9
6 SGTC_37 0566-0070 5.89 1.6 µM 2.18E-8 6 1.90E-9
7 SGTC_1333 1482-0047 5.39 286.0 µM 2.73E-7 6 3.55E-8
8 SGTC_2296 7971656 5.25 126.5 µM 5.25E-7 20 7.55E-8
9 SGTC_2666 tannic acid 5.20 100.0 µM 6.76E-7 iron homeostasis 17 1.01E-7
10 SGTC_2679 rapamycin 5.05 1.0 nM 1.35E-6 95 2.26E-7
11 SGTC_214 4373-3496 5.04 70.0 µM 1.42E-6 28 2.39E-7
12 SGTC_2737 bleomycin 4.99 30.5 nM 1.72E-6 Golgi 54 2.97E-7
13 SGTC_1260 0700-1617 4.83 7.8 µM 3.52E-6 calcium & mitochondrial duress 17 6.82E-7
14 SGTC_255 tunicamycin 4.81 200.5 nM 3.87E-6 unfolded protein response 47 7.60E-7
15 SGTC_117 lucigenin 4.77 22.1 µM 4.66E-6 redox potentiating 64 9.42E-7
16 SGTC_2415 st077333 4.73 57.7 µM 5.43E-6 24 1.12E-6
17 SGTC_2360 9040895 4.72 200.0 µM 5.60E-6 RPP1 & pyrimidine depletion 11 1.16E-6
18 SGTC_2302 7771753 4.67 200.0 µM 7.12E-6 24 1.54E-6
19 SGTC_108 0509-0257 4.64 621.1 µM 8.07E-6 31 1.78E-6
20 SGTC_3157 9099274 4.63 49.5 µM 8.26E-6 12 1.82E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.319 8.66E-80 YNR006W VPS27 hom Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
0.318 2.13E-79 YKL041W VPS24 hom One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
0.309 5.44E-75 YPR173C VPS4 hom AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
0.253 6.27E-50 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.236 8.48E-44 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.211 4.16E-35 YKR035C_d OPI8_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A
0.209 1.86E-34 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.207 8.06E-34 YKR035W-A DID2 hom Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
0.190 9.88E-29 YPL084W BRO1 hom Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
0.190 1.69E-28 YDR486C VPS60 hom Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p
0.184 7.51E-27 YPL065W VPS28 hom Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
0.182 2.55E-26 YMR077C VPS20 hom Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes
0.175 1.38E-24 YAL002W VPS8 hom Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
0.170 4.90E-23 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.159 2.08E-20 YOR089C VPS21 hom Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication