YKL003C / MRP17

Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL003C / MRP17

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:4.69|P-value:3.13E-6 ID:SGTC_19|Compound:0279-0214|FD-Score:6.20|P-value:1.19E-9 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:4.77|P-value:2.21E-6 ID:SGTC_29|Compound:0299-0004|FD-Score:-4.63|P-value:4.16E-6 ID:SGTC_66|Compound:0109-0187|FD-Score:4.07|P-value:4.38E-5 ID:SGTC_89|Compound:0302-0250|FD-Score:3.30|P-value:7.44E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:3.52|P-value:3.47E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:3.89|P-value:8.96E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:3.51|P-value:3.68E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:3.53|P-value:3.38E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.59|P-value:2.77E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.12|P-value:3.55E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:-3.31|P-value:7.04E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:4.82|P-value:1.74E-6 ID:SGTC_1199|Compound:1638-0010|FD-Score:-3.50|P-value:3.67E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.56|P-value:3.00E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:3.22|P-value:9.66E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:3.32|P-value:6.84E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.99|P-value:6.18E-5 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:3.38|P-value:5.75E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.83|P-value:1.11E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-5.52|P-value:5.23E-8 ID:SGTC_1832|Compound:st054113|FD-Score:5.00|P-value:7.52E-7 ID:SGTC_1842|Compound:st055362|FD-Score:-3.29|P-value:7.71E-4 ID:SGTC_2125|Compound:5246583|FD-Score:5.66|P-value:2.49E-8 ID:SGTC_2376|Compound:9072922|FD-Score:3.28|P-value:7.85E-4 ID:SGTC_2411|Compound:st077133|FD-Score:4.03|P-value:5.23E-5 ID:SGTC_2441|Compound:5730946|FD-Score:3.57|P-value:2.90E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.52|P-value:3.47E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:-3.44|P-value:4.56E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:3.48|P-value:3.98E-4 ID:SGTC_2748|Compound:diazoxide|FD-Score:3.49|P-value:3.96E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.87|P-value:9.68E-5 ID:SGTC_2781|Compound:5567276|FD-Score:-6.41|P-value:3.26E-10 ID:SGTC_2782|Compound:7736369|FD-Score:-3.95|P-value:6.93E-5 ID:SGTC_3049|Compound:9092223|FD-Score:3.77|P-value:1.43E-4 ID:SGTC_3069|Compound:9117009|FD-Score:3.64|P-value:2.32E-4 ID:SGTC_3141|Compound:9094609|FD-Score:-3.26|P-value:8.42E-4 ID:SGTC_3145|Compound:9085516|FD-Score:-3.50|P-value:3.66E-4 ID:SGTC_3314|Compound:9135860|FD-Score:3.58|P-value:2.87E-4 ID:SGTC_10|Compound:0279-0212|FD-Score:4.69|P-value:3.13E-6 ID:SGTC_19|Compound:0279-0214|FD-Score:6.20|P-value:1.19E-9 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:4.77|P-value:2.21E-6 ID:SGTC_29|Compound:0299-0004|FD-Score:-4.63|P-value:4.16E-6 ID:SGTC_66|Compound:0109-0187|FD-Score:4.07|P-value:4.38E-5 ID:SGTC_89|Compound:0302-0250|FD-Score:3.30|P-value:7.44E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:3.52|P-value:3.47E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:3.89|P-value:8.96E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:3.51|P-value:3.68E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:3.53|P-value:3.38E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.59|P-value:2.77E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.12|P-value:3.55E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:-3.31|P-value:7.04E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:4.82|P-value:1.74E-6 ID:SGTC_1199|Compound:1638-0010|FD-Score:-3.50|P-value:3.67E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.56|P-value:3.00E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:3.22|P-value:9.66E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:3.32|P-value:6.84E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.99|P-value:6.18E-5 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:3.38|P-value:5.75E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.83|P-value:1.11E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-5.52|P-value:5.23E-8 ID:SGTC_1832|Compound:st054113|FD-Score:5.00|P-value:7.52E-7 ID:SGTC_1842|Compound:st055362|FD-Score:-3.29|P-value:7.71E-4 ID:SGTC_2125|Compound:5246583|FD-Score:5.66|P-value:2.49E-8 ID:SGTC_2376|Compound:9072922|FD-Score:3.28|P-value:7.85E-4 ID:SGTC_2411|Compound:st077133|FD-Score:4.03|P-value:5.23E-5 ID:SGTC_2441|Compound:5730946|FD-Score:3.57|P-value:2.90E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.52|P-value:3.47E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:-3.44|P-value:4.56E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:3.48|P-value:3.98E-4 ID:SGTC_2748|Compound:diazoxide|FD-Score:3.49|P-value:3.96E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.87|P-value:9.68E-5 ID:SGTC_2781|Compound:5567276|FD-Score:-6.41|P-value:3.26E-10 ID:SGTC_2782|Compound:7736369|FD-Score:-3.95|P-value:6.93E-5 ID:SGTC_3049|Compound:9092223|FD-Score:3.77|P-value:1.43E-4 ID:SGTC_3069|Compound:9117009|FD-Score:3.64|P-value:2.32E-4 ID:SGTC_3141|Compound:9094609|FD-Score:-3.26|P-value:8.42E-4 ID:SGTC_3145|Compound:9085516|FD-Score:-3.50|P-value:3.66E-4 ID:SGTC_3314|Compound:9135860|FD-Score:3.58|P-value:2.87E-4

Top fitness defect scores for YKL003C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_19 0279-0214 6.20 38.4 µM 1.19E-9 TRP & mitochondrial translation 8 2.85E-10
2 SGTC_2125 5246583 5.66 200.0 µM 2.49E-8 tubulin folding & SWR complex 18 7.49E-9
3 SGTC_1832 st054113 5.00 55.0 µM 7.52E-7 3 2.93E-7
4 SGTC_1108 betulinic acid 4.82 729.1 nM 1.74E-6 6 7.21E-7
5 SGTC_28 pentachloropyridine N-oxide 4.77 1.6 µM 2.21E-6 TSC3-RPN4 35 9.33E-7
6 SGTC_10 0279-0212 4.69 42.6 µM 3.13E-6 TRP & mitochondrial translation 75 1.36E-6
7 SGTC_66 0109-0187 4.07 42.4 µM 4.38E-5 38 2.31E-5
8 SGTC_2411 st077133 4.03 39.9 µM 5.23E-5 RNA pol III & RNase P/MRP 31 2.80E-5
9 SGTC_1597 7-benzyloxygramine 3.99 47.5 µM 6.18E-5 calcium & mitochondrial duress 118 3.34E-5
10 SGTC_173 1273-0061 3.89 2.7 µM 8.96E-5 mitochondrial stress 190 4.98E-5
11 SGTC_3049 9092223 3.77 49.5 µM 1.43E-4 28 8.21E-5
12 SGTC_3069 9117009 3.64 49.5 µM 2.32E-4 60S ribosome export 11 1.39E-4
13 SGTC_310 1273-0058 3.59 1.1 µM 2.77E-4 mitochondrial stress 278 1.67E-4
14 SGTC_3314 9135860 3.58 59.4 µM 2.87E-4 26 1.74E-4
15 SGTC_2441 5730946 3.57 39.1 µM 2.90E-4 30 1.76E-4
16 SGTC_275 trichlorophene 3.53 5.7 µM 3.38E-4 mitochondrial stress 248 2.07E-4
17 SGTC_118 0083-0097 3.52 135.3 µM 3.47E-4 translation 182 2.14E-4
18 SGTC_236 1509-0011 3.51 5.3 µM 3.68E-4 417 2.27E-4
19 SGTC_2748 diazoxide 3.49 86.7 µM 3.96E-4 17 2.46E-4
20 SGTC_2607 solasodine 3.48 47.4 µM 3.98E-4 79 2.47E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.331 1.42E-86 YER087W AIM10 hom Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss
0.308 6.48E-75 YPL040C ISM1 hom Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
0.306 1.41E-73 YMR228W MTF1 hom Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.261 1.74E-53 YDR194C MSS116 hom DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing
0.259 1.26E-52 YER050C RSM18 hom Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein
0.256 3.33E-51 YMR193W MRPL24 hom Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
0.255 7.40E-51 YER077C_p YER077C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport
0.254 1.97E-50 YDR079W PET100 hom Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme
0.252 6.24E-50 YOL095C HMI1 hom Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD
0.252 2.16E-49 YMR287C DSS1 hom 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.251 3.37E-49 YHR168W MTG2 hom Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly
0.250 8.13E-49 YCR046C IMG1 hom Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome
0.249 1.43E-48 YGR220C MRPL9 hom Mitochondrial ribosomal protein of the large subunit
0.247 1.06E-47 YIL060W_p YIL060W_p hom Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene
0.235 2.09E-43 YPR100W MRPL51 hom Mitochondrial ribosomal protein of the large subunit