YKL008C / LAC1

Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL008C / LAC1

Click on Significant Values for Screen Details ID:SGTC_113|Compound:0099-0221|FD-Score:-4.35|P-value:3.40E-6 ID:SGTC_175|Compound:3793-3120|FD-Score:3.21|P-value:4.44E-4 ID:SGTC_183|Compound:3914-0051|FD-Score:3.46|P-value:1.75E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:-3.09|P-value:6.85E-4 ID:SGTC_252|Compound:miconazole|FD-Score:-4.60|P-value:9.66E-7 ID:SGTC_277|Compound:nystatin|FD-Score:4.03|P-value:1.52E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.05|P-value:7.88E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-7.33|P-value:1.64E-14 ID:SGTC_517|Compound:1365-0151|FD-Score:3.05|P-value:8.05E-4 ID:SGTC_569|Compound:4130-2812|FD-Score:-3.69|P-value:6.67E-5 ID:SGTC_612|Compound:0302-0167|FD-Score:-3.39|P-value:2.27E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:3.03|P-value:8.53E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:3.09|P-value:6.91E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:3.75|P-value:5.32E-5 ID:SGTC_801|Compound:0866-0028|FD-Score:3.09|P-value:7.08E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:3.85|P-value:3.50E-5 ID:SGTC_876|Compound:0720-0963|FD-Score:-3.94|P-value:2.29E-5 ID:SGTC_935|Compound:2996-0153|FD-Score:3.21|P-value:4.47E-4 ID:SGTC_984|Compound:1492-0255|FD-Score:3.11|P-value:6.53E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:4.10|P-value:1.12E-5 ID:SGTC_1661|Compound:st013063|FD-Score:4.46|P-value:1.94E-6 ID:SGTC_1812|Compound:st052057|FD-Score:3.84|P-value:3.58E-5 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:3.99|P-value:1.82E-5 ID:SGTC_1876|Compound:myriocin|FD-Score:4.68|P-value:6.41E-7 ID:SGTC_1958|Compound:st076513|FD-Score:3.55|P-value:1.22E-4 ID:SGTC_2053|Compound:5265679|FD-Score:-3.54|P-value:1.22E-4 ID:SGTC_2120|Compound:5538279|FD-Score:3.19|P-value:4.94E-4 ID:SGTC_2180|Compound:6301957|FD-Score:-3.01|P-value:9.04E-4 ID:SGTC_2205|Compound:7238770|FD-Score:-3.32|P-value:2.95E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:-3.15|P-value:5.43E-4 ID:SGTC_2265|Compound:7929201|FD-Score:3.02|P-value:9.07E-4 ID:SGTC_2266|Compound:7929956|FD-Score:3.13|P-value:5.96E-4 ID:SGTC_2339|Compound:9000433|FD-Score:3.26|P-value:3.73E-4 ID:SGTC_2426|Compound:5582046|FD-Score:3.26|P-value:3.74E-4 ID:SGTC_2738|Compound:aclarubicin|FD-Score:3.86|P-value:3.32E-5 ID:SGTC_2753|Compound:fluvastatin|FD-Score:4.48|P-value:1.78E-6 ID:SGTC_2755|Compound:lomerizine|FD-Score:-5.70|P-value:1.75E-9 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.65|P-value:8.12E-5 ID:SGTC_2781|Compound:5567276|FD-Score:3.55|P-value:1.21E-4 ID:SGTC_2782|Compound:7736369|FD-Score:-4.13|P-value:9.29E-6 ID:SGTC_2787|Compound:5284540|FD-Score:-5.41|P-value:1.04E-8 ID:SGTC_2790|Compound:5423970|FD-Score:-3.95|P-value:2.14E-5 ID:SGTC_2810|Compound:7959871|FD-Score:3.15|P-value:5.54E-4 ID:SGTC_3090|Compound:9117696|FD-Score:4.90|P-value:2.05E-7 ID:SGTC_3091|Compound:9116372|FD-Score:3.34|P-value:2.74E-4 ID:SGTC_3104|Compound:9120930|FD-Score:3.92|P-value:2.50E-5 ID:SGTC_3136|Compound:9095672|FD-Score:3.16|P-value:5.51E-4 ID:SGTC_3151|Compound:9099821|FD-Score:4.86|P-value:2.44E-7 ID:SGTC_3248|Compound:9135562|FD-Score:-3.93|P-value:2.39E-5 ID:SGTC_113|Compound:0099-0221|FD-Score:-4.35|P-value:3.40E-6 ID:SGTC_175|Compound:3793-3120|FD-Score:3.21|P-value:4.44E-4 ID:SGTC_183|Compound:3914-0051|FD-Score:3.46|P-value:1.75E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:-3.09|P-value:6.85E-4 ID:SGTC_252|Compound:miconazole|FD-Score:-4.60|P-value:9.66E-7 ID:SGTC_277|Compound:nystatin|FD-Score:4.03|P-value:1.52E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.05|P-value:7.88E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-7.33|P-value:1.64E-14 ID:SGTC_517|Compound:1365-0151|FD-Score:3.05|P-value:8.05E-4 ID:SGTC_569|Compound:4130-2812|FD-Score:-3.69|P-value:6.67E-5 ID:SGTC_612|Compound:0302-0167|FD-Score:-3.39|P-value:2.27E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:3.03|P-value:8.53E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:3.09|P-value:6.91E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:3.75|P-value:5.32E-5 ID:SGTC_801|Compound:0866-0028|FD-Score:3.09|P-value:7.08E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:3.85|P-value:3.50E-5 ID:SGTC_876|Compound:0720-0963|FD-Score:-3.94|P-value:2.29E-5 ID:SGTC_935|Compound:2996-0153|FD-Score:3.21|P-value:4.47E-4 ID:SGTC_984|Compound:1492-0255|FD-Score:3.11|P-value:6.53E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:4.10|P-value:1.12E-5 ID:SGTC_1661|Compound:st013063|FD-Score:4.46|P-value:1.94E-6 ID:SGTC_1812|Compound:st052057|FD-Score:3.84|P-value:3.58E-5 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:3.99|P-value:1.82E-5 ID:SGTC_1876|Compound:myriocin|FD-Score:4.68|P-value:6.41E-7 ID:SGTC_1958|Compound:st076513|FD-Score:3.55|P-value:1.22E-4 ID:SGTC_2053|Compound:5265679|FD-Score:-3.54|P-value:1.22E-4 ID:SGTC_2120|Compound:5538279|FD-Score:3.19|P-value:4.94E-4 ID:SGTC_2180|Compound:6301957|FD-Score:-3.01|P-value:9.04E-4 ID:SGTC_2205|Compound:7238770|FD-Score:-3.32|P-value:2.95E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:-3.15|P-value:5.43E-4 ID:SGTC_2265|Compound:7929201|FD-Score:3.02|P-value:9.07E-4 ID:SGTC_2266|Compound:7929956|FD-Score:3.13|P-value:5.96E-4 ID:SGTC_2339|Compound:9000433|FD-Score:3.26|P-value:3.73E-4 ID:SGTC_2426|Compound:5582046|FD-Score:3.26|P-value:3.74E-4 ID:SGTC_2738|Compound:aclarubicin|FD-Score:3.86|P-value:3.32E-5 ID:SGTC_2753|Compound:fluvastatin|FD-Score:4.48|P-value:1.78E-6 ID:SGTC_2755|Compound:lomerizine|FD-Score:-5.70|P-value:1.75E-9 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.65|P-value:8.12E-5 ID:SGTC_2781|Compound:5567276|FD-Score:3.55|P-value:1.21E-4 ID:SGTC_2782|Compound:7736369|FD-Score:-4.13|P-value:9.29E-6 ID:SGTC_2787|Compound:5284540|FD-Score:-5.41|P-value:1.04E-8 ID:SGTC_2790|Compound:5423970|FD-Score:-3.95|P-value:2.14E-5 ID:SGTC_2810|Compound:7959871|FD-Score:3.15|P-value:5.54E-4 ID:SGTC_3090|Compound:9117696|FD-Score:4.90|P-value:2.05E-7 ID:SGTC_3091|Compound:9116372|FD-Score:3.34|P-value:2.74E-4 ID:SGTC_3104|Compound:9120930|FD-Score:3.92|P-value:2.50E-5 ID:SGTC_3136|Compound:9095672|FD-Score:3.16|P-value:5.51E-4 ID:SGTC_3151|Compound:9099821|FD-Score:4.86|P-value:2.44E-7 ID:SGTC_3248|Compound:9135562|FD-Score:-3.93|P-value:2.39E-5

Top fitness defect scores for YKL008C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3090 9117696 4.90 49.5 µM 2.05E-7 8 4.88E-7
2 SGTC_3151 9099821 4.86 49.5 µM 2.44E-7 8 5.74E-7
3 SGTC_1876 myriocin 4.68 605.0 nM 6.41E-7 22 1.42E-6
4 SGTC_2753 fluvastatin 4.48 17.1 µM 1.78E-6 azole & statin 20 3.70E-6
5 SGTC_1661 st013063 4.46 9.7 µM 1.94E-6 19 4.01E-6
6 SGTC_1257 0669-0121 4.10 16.0 µM 1.12E-5 22 2.07E-5
7 SGTC_277 nystatin 4.03 15.5 µM 1.52E-5 12 2.75E-5
8 SGTC_1869 3',7'-dimethoxyflavone 3.99 40.3 µM 1.82E-5 22 3.27E-5
9 SGTC_3104 9120930 3.92 49.5 µM 2.50E-5 11 4.39E-5
10 SGTC_2738 aclarubicin 3.86 5.5 µM 3.32E-5 RNA pol III & RNase P/MRP 9 5.74E-5
11 SGTC_872 0588-0139 3.85 234.0 µM 3.50E-5 36 6.03E-5
12 SGTC_1812 st052057 3.84 4.3 µM 3.58E-5 calcium & mitochondrial duress 28 6.15E-5
13 SGTC_779 1360-0071 3.75 233.0 µM 5.32E-5 fatty acid desaturase (OLE1) 36 8.92E-5
14 SGTC_2766 oltipraz 3.65 83.9 µM 8.12E-5 heme requiring 37 1.33E-4
15 SGTC_2781 5567276 3.55 71.4 µM 1.21E-4 69 1.93E-4
16 SGTC_1958 st076513 3.55 41.9 µM 1.22E-4 60S ribosome export 43 1.94E-4
17 SGTC_183 3914-0051 3.46 30.6 µM 1.75E-4 60S ribosome export 36 2.72E-4
18 SGTC_3091 9116372 3.34 49.5 µM 2.74E-4 34 4.16E-4
19 SGTC_2339 9000433 3.26 200.0 µM 3.73E-4 62 5.55E-4
20 SGTC_2426 5582046 3.26 32.2 µM 3.74E-4 54 5.56E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.145 3.78E-17 YGR212W SLI1 hom N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
0.110 1.87E-10 YDR071C PAA1 hom Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication
0.110 2.01E-10 YML006C GIS4 hom CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway
0.105 1.15E-9 YMR298W LIP1 het Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis
0.093 5.77E-8 YBR125C PTC4 hom Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity
0.093 6.52E-8 YKL117W SBA1 hom Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress
0.084 1.09E-6 YMR296C LCB1 het Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
0.081 2.81E-6 YMR304C-A_d YMR304C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10
0.076 9.13E-6 YOR312C RPL20B hom Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication
0.075 1.57E-5 YMR292W GOT1 hom Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition
0.073 2.15E-5 YER016W BIM1 hom Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally
0.073 2.56E-5 YGR213C RTA1 hom Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein
0.072 3.36E-5 YGL230C_p YGL230C_p hom Putative protein of unknown function; non-essential gene
0.069 5.96E-5 YER048C CAJ1 hom Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly
0.068 8.13E-5 YDR094W_d YDR094W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2