YKL009W / MRT4

Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL009W / MRT4

Click on Significant Values for Screen Details ID:SGTC_25|Compound:0118-0223|FD-Score:3.29|P-value:7.58E-4 ID:SGTC_33|Compound:0986-0033|FD-Score:4.04|P-value:4.77E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:4.69|P-value:2.78E-6 ID:SGTC_118|Compound:0083-0097|FD-Score:5.77|P-value:1.16E-8 ID:SGTC_297|Compound:0986-0032|FD-Score:3.54|P-value:3.18E-4 ID:SGTC_446|Compound:go6976|FD-Score:-3.22|P-value:7.64E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:-5.77|P-value:8.01E-9 ID:SGTC_604|Compound:1000-0114|FD-Score:-3.42|P-value:3.90E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:3.61|P-value:2.43E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.53|P-value:3.32E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:-9.46|P-value:1.19E-20 ID:SGTC_781|Compound:4335-2806|FD-Score:-3.57|P-value:2.28E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:5.51|P-value:4.80E-8 ID:SGTC_786|Compound:4544-0069|FD-Score:3.37|P-value:5.69E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:4.37|P-value:1.20E-5 ID:SGTC_820|Compound:1582-0060|FD-Score:3.23|P-value:9.03E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:3.78|P-value:1.31E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:3.55|P-value:3.00E-4 ID:SGTC_846|Compound:0335-0850|FD-Score:-3.31|P-value:5.73E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:3.41|P-value:5.01E-4 ID:SGTC_856|Compound:0433-0016|FD-Score:3.31|P-value:7.11E-4 ID:SGTC_897|Compound:1000-0139|FD-Score:-4.28|P-value:1.34E-5 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.80|P-value:9.42E-5 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.38|P-value:5.44E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.77|P-value:1.35E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:6.84|P-value:1.66E-11 ID:SGTC_1446|Compound:4239-0190|FD-Score:4.43|P-value:8.98E-6 ID:SGTC_1454|Compound:4239-0054|FD-Score:5.01|P-value:6.11E-7 ID:SGTC_1457|Compound:k015-0032|FD-Score:5.77|P-value:1.13E-8 ID:SGTC_1461|Compound:k072-0203|FD-Score:3.79|P-value:1.26E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:-3.27|P-value:6.45E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:-3.57|P-value:2.24E-4 ID:SGTC_1874|Compound:staurosporine|FD-Score:-3.60|P-value:2.05E-4 ID:SGTC_1953|Compound:st076800|FD-Score:3.30|P-value:7.19E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.75|P-value:1.48E-4 ID:SGTC_2252|Compound:omeprazole|FD-Score:3.46|P-value:4.13E-4 ID:SGTC_2330|Compound:7979373|FD-Score:3.34|P-value:6.25E-4 ID:SGTC_2375|Compound:9072683|FD-Score:3.26|P-value:8.39E-4 ID:SGTC_2399|Compound:5884133|FD-Score:4.31|P-value:1.55E-5 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:-6.63|P-value:4.39E-11 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:3.52|P-value:3.36E-4 ID:SGTC_2628|Compound:lathosterol|FD-Score:4.53|P-value:5.77E-6 ID:SGTC_2943|Compound:9068529|FD-Score:-4.98|P-value:5.17E-7 ID:SGTC_3089|Compound:9117425|FD-Score:3.81|P-value:1.18E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-4.08|P-value:3.11E-5 ID:SGTC_3201|Compound:9113999|FD-Score:-3.33|P-value:5.23E-4 ID:SGTC_3204|Compound:9114183|FD-Score:6.58|P-value:8.81E-11 ID:SGTC_3208|Compound:9114115|FD-Score:-4.37|P-value:9.03E-6 ID:SGTC_3225|Compound:9130819|FD-Score:-4.00|P-value:4.24E-5 ID:SGTC_3235|Compound:9132602|FD-Score:-3.61|P-value:1.94E-4 ID:SGTC_25|Compound:0118-0223|FD-Score:3.29|P-value:7.58E-4 ID:SGTC_33|Compound:0986-0033|FD-Score:4.04|P-value:4.77E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:4.69|P-value:2.78E-6 ID:SGTC_118|Compound:0083-0097|FD-Score:5.77|P-value:1.16E-8 ID:SGTC_297|Compound:0986-0032|FD-Score:3.54|P-value:3.18E-4 ID:SGTC_446|Compound:go6976|FD-Score:-3.22|P-value:7.64E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:-5.77|P-value:8.01E-9 ID:SGTC_604|Compound:1000-0114|FD-Score:-3.42|P-value:3.90E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:3.61|P-value:2.43E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.53|P-value:3.32E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:-9.46|P-value:1.19E-20 ID:SGTC_781|Compound:4335-2806|FD-Score:-3.57|P-value:2.28E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:5.51|P-value:4.80E-8 ID:SGTC_786|Compound:4544-0069|FD-Score:3.37|P-value:5.69E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:4.37|P-value:1.20E-5 ID:SGTC_820|Compound:1582-0060|FD-Score:3.23|P-value:9.03E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:3.78|P-value:1.31E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:3.55|P-value:3.00E-4 ID:SGTC_846|Compound:0335-0850|FD-Score:-3.31|P-value:5.73E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:3.41|P-value:5.01E-4 ID:SGTC_856|Compound:0433-0016|FD-Score:3.31|P-value:7.11E-4 ID:SGTC_897|Compound:1000-0139|FD-Score:-4.28|P-value:1.34E-5 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.80|P-value:9.42E-5 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.38|P-value:5.44E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.77|P-value:1.35E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:6.84|P-value:1.66E-11 ID:SGTC_1446|Compound:4239-0190|FD-Score:4.43|P-value:8.98E-6 ID:SGTC_1454|Compound:4239-0054|FD-Score:5.01|P-value:6.11E-7 ID:SGTC_1457|Compound:k015-0032|FD-Score:5.77|P-value:1.13E-8 ID:SGTC_1461|Compound:k072-0203|FD-Score:3.79|P-value:1.26E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:-3.27|P-value:6.45E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:-3.57|P-value:2.24E-4 ID:SGTC_1874|Compound:staurosporine|FD-Score:-3.60|P-value:2.05E-4 ID:SGTC_1953|Compound:st076800|FD-Score:3.30|P-value:7.19E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.75|P-value:1.48E-4 ID:SGTC_2252|Compound:omeprazole|FD-Score:3.46|P-value:4.13E-4 ID:SGTC_2330|Compound:7979373|FD-Score:3.34|P-value:6.25E-4 ID:SGTC_2375|Compound:9072683|FD-Score:3.26|P-value:8.39E-4 ID:SGTC_2399|Compound:5884133|FD-Score:4.31|P-value:1.55E-5 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:-6.63|P-value:4.39E-11 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:3.52|P-value:3.36E-4 ID:SGTC_2628|Compound:lathosterol|FD-Score:4.53|P-value:5.77E-6 ID:SGTC_2943|Compound:9068529|FD-Score:-4.98|P-value:5.17E-7 ID:SGTC_3089|Compound:9117425|FD-Score:3.81|P-value:1.18E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-4.08|P-value:3.11E-5 ID:SGTC_3201|Compound:9113999|FD-Score:-3.33|P-value:5.23E-4 ID:SGTC_3204|Compound:9114183|FD-Score:6.58|P-value:8.81E-11 ID:SGTC_3208|Compound:9114115|FD-Score:-4.37|P-value:9.03E-6 ID:SGTC_3225|Compound:9130819|FD-Score:-4.00|P-value:4.24E-5 ID:SGTC_3235|Compound:9132602|FD-Score:-3.61|P-value:1.94E-4

Top fitness defect scores for YKL009W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1261 0712-0010 6.84 12.7 µM 1.66E-11 RNA processing & uracil transport 3 3.98E-12
2 SGTC_3204 9114183 6.58 49.5 µM 8.81E-11 6 2.33E-11
3 SGTC_1457 k015-0032 5.77 57.3 µM 1.13E-8 8 3.94E-9
4 SGTC_118 0083-0097 5.77 135.3 µM 1.16E-8 translation 32 4.05E-9
5 SGTC_782 0083-0095 5.51 30.5 µM 4.80E-8 7 1.82E-8
6 SGTC_1454 4239-0054 5.01 42.6 µM 6.11E-7 9 2.69E-7
7 SGTC_103 0844-0104 4.69 51.8 µM 2.78E-6 ERAD & cell cycle 111 1.34E-6
8 SGTC_2628 lathosterol 4.53 100.0 µM 5.77E-6 18 2.90E-6
9 SGTC_1446 4239-0190 4.43 62.0 µM 8.98E-6 25 4.63E-6
10 SGTC_790 1575-0012 4.37 79.1 µM 1.20E-5 TSC3-RPN4 18 6.28E-6
11 SGTC_2399 5884133 4.31 200.0 µM 1.55E-5 cell wall signaling 59 8.26E-6
12 SGTC_33 0986-0033 4.04 35.8 µM 4.77E-5 calcium & mitochondrial duress 18 2.71E-5
13 SGTC_3089 9117425 3.81 49.5 µM 1.18E-4 25 7.05E-5
14 SGTC_1461 k072-0203 3.79 53.8 µM 1.26E-4 16 7.55E-5
15 SGTC_831 k061-1017 3.78 217.0 µM 1.31E-4 translation 74 7.92E-5
16 SGTC_1254 0550-0035 3.77 264.0 µM 1.35E-4 24 8.14E-5
17 SGTC_1998 st075797 3.75 76.8 µM 1.48E-4 mitochondrial response to ROS 20 8.98E-5
18 SGTC_625 k213-0058 3.61 121.0 µM 2.43E-4 translation 135 1.52E-4
19 SGTC_832 4083-0019 3.55 164.0 µM 3.00E-4 translation 129 1.90E-4
20 SGTC_297 0986-0032 3.54 24.0 µM 3.18E-4 60S ribosome export 14 2.02E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.149 4.44E-18 YLR061W RPL22A hom Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
0.117 1.17E-11 YLR264C-A_p YLR264C-A_p hom Putative protein of unknown function
0.113 4.65E-11 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.102 3.23E-9 YKL180W RPL17A het Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.099 8.39E-9 YBR225W_p YBR225W_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components
0.099 1.08E-8 YBL093C ROX3 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme
0.094 4.62E-8 YOR369C RPS12 hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog
0.093 6.72E-8 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.093 7.64E-8 YHR010W RPL27A hom Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication
0.092 1.00E-7 YJL049W_p YJL049W_p hom Putative protein of unknown function; YJL049W is a non-essential gene
0.091 1.22E-7 YGR162W TIF4631 hom Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication
0.091 1.32E-7 YGR271C-A EFG1 hom Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
0.086 5.31E-7 YNL227C JJJ1 hom Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis
0.086 5.78E-7 YJL188C_d BUD19_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.086 6.77E-7 YGL246C RAI1 hom Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z