YKL017C / HCS1

Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL017C / HCS1

Click on Significant Values for Screen Details ID:SGTC_70|Compound:0141-0289|FD-Score:-3.58|P-value:2.02E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:3.00|P-value:6.65E-4 ID:SGTC_343|Compound:4130-2827|FD-Score:4.02|P-value:1.02E-5 ID:SGTC_358|Compound:0075-0058|FD-Score:3.52|P-value:9.08E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:2.96|P-value:7.67E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:3.46|P-value:1.18E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:-3.95|P-value:4.43E-5 ID:SGTC_469|Compound:w7|FD-Score:-3.16|P-value:9.41E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:3.09|P-value:4.85E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:3.94|P-value:1.51E-5 ID:SGTC_756|Compound:0508-1999|FD-Score:3.09|P-value:4.87E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:3.05|P-value:5.51E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:3.81|P-value:2.63E-5 ID:SGTC_909|Compound:3448-9333|FD-Score:-3.56|P-value:2.12E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:5.05|P-value:5.54E-8 ID:SGTC_914|Compound:3820-6779|FD-Score:-4.05|P-value:2.80E-5 ID:SGTC_1158|Compound:4092-0494|FD-Score:2.98|P-value:7.19E-4 ID:SGTC_1228|Compound:0124-0032|FD-Score:2.96|P-value:7.75E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.45|P-value:3.32E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:-3.67|P-value:1.37E-4 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.60|P-value:6.44E-5 ID:SGTC_1285|Compound:0929-0069|FD-Score:3.74|P-value:3.58E-5 ID:SGTC_1312|Compound:1218-2183|FD-Score:3.07|P-value:5.12E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.88|P-value:1.96E-5 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.61|P-value:6.28E-5 ID:SGTC_1704|Compound:st028805|FD-Score:3.85|P-value:2.19E-5 ID:SGTC_1779|Compound:st039427|FD-Score:4.97|P-value:8.45E-8 ID:SGTC_1837|Compound:st055472|FD-Score:3.14|P-value:3.96E-4 ID:SGTC_1845|Compound:st056753|FD-Score:6.09|P-value:8.76E-11 ID:SGTC_1847|Compound:st056231|FD-Score:-3.91|P-value:5.22E-5 ID:SGTC_1854|Compound:st056233|FD-Score:3.18|P-value:3.47E-4 ID:SGTC_1855|Compound:st056195|FD-Score:3.11|P-value:4.42E-4 ID:SGTC_1923|Compound:st058458|FD-Score:4.08|P-value:7.94E-6 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.15|P-value:1.81E-5 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:3.06|P-value:5.36E-4 ID:SGTC_1934|Compound:st074720|FD-Score:3.25|P-value:2.60E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.36|P-value:1.76E-4 ID:SGTC_2113|Compound:5491891|FD-Score:-3.14|P-value:9.83E-4 ID:SGTC_2124|Compound:5246320|FD-Score:4.42|P-value:1.53E-6 ID:SGTC_2290|Compound:7904846|FD-Score:3.07|P-value:5.25E-4 ID:SGTC_2318|Compound:6029776|FD-Score:3.16|P-value:3.76E-4 ID:SGTC_2452|Compound:5863136|FD-Score:3.13|P-value:4.15E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:2.93|P-value:8.57E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:5.13|P-value:3.51E-8 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:3.44|P-value:1.26E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:4.61|P-value:5.71E-7 ID:SGTC_2586|Compound:curcumin|FD-Score:4.12|P-value:6.50E-6 ID:SGTC_2846|Compound:9008642|FD-Score:3.21|P-value:3.08E-4 ID:SGTC_2859|Compound:9028643|FD-Score:3.62|P-value:6.13E-5 ID:SGTC_2950|Compound:9072895|FD-Score:3.75|P-value:3.47E-5 ID:SGTC_2975|Compound:9084847|FD-Score:-3.74|P-value:1.03E-4 ID:SGTC_2981|Compound:9013952|FD-Score:4.60|P-value:6.11E-7 ID:SGTC_2986|Compound:9022445|FD-Score:10.30|P-value:7.69E-27 ID:SGTC_3071|Compound:9117417|FD-Score:5.54|P-value:2.93E-9 ID:SGTC_3073|Compound:9114972|FD-Score:4.52|P-value:9.04E-7 ID:SGTC_3074|Compound:9116018|FD-Score:-4.35|P-value:7.43E-6 ID:SGTC_3104|Compound:9120930|FD-Score:-3.32|P-value:5.35E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.25|P-value:2.68E-4 ID:SGTC_3232|Compound:9132874|FD-Score:5.15|P-value:3.03E-8 ID:SGTC_3277|Compound:9138724|FD-Score:3.01|P-value:6.53E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:-3.58|P-value:2.02E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:3.00|P-value:6.65E-4 ID:SGTC_343|Compound:4130-2827|FD-Score:4.02|P-value:1.02E-5 ID:SGTC_358|Compound:0075-0058|FD-Score:3.52|P-value:9.08E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:2.96|P-value:7.67E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:3.46|P-value:1.18E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:-3.95|P-value:4.43E-5 ID:SGTC_469|Compound:w7|FD-Score:-3.16|P-value:9.41E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:3.09|P-value:4.85E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:3.94|P-value:1.51E-5 ID:SGTC_756|Compound:0508-1999|FD-Score:3.09|P-value:4.87E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:3.05|P-value:5.51E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:3.81|P-value:2.63E-5 ID:SGTC_909|Compound:3448-9333|FD-Score:-3.56|P-value:2.12E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:5.05|P-value:5.54E-8 ID:SGTC_914|Compound:3820-6779|FD-Score:-4.05|P-value:2.80E-5 ID:SGTC_1158|Compound:4092-0494|FD-Score:2.98|P-value:7.19E-4 ID:SGTC_1228|Compound:0124-0032|FD-Score:2.96|P-value:7.75E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.45|P-value:3.32E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:-3.67|P-value:1.37E-4 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.60|P-value:6.44E-5 ID:SGTC_1285|Compound:0929-0069|FD-Score:3.74|P-value:3.58E-5 ID:SGTC_1312|Compound:1218-2183|FD-Score:3.07|P-value:5.12E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.88|P-value:1.96E-5 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.61|P-value:6.28E-5 ID:SGTC_1704|Compound:st028805|FD-Score:3.85|P-value:2.19E-5 ID:SGTC_1779|Compound:st039427|FD-Score:4.97|P-value:8.45E-8 ID:SGTC_1837|Compound:st055472|FD-Score:3.14|P-value:3.96E-4 ID:SGTC_1845|Compound:st056753|FD-Score:6.09|P-value:8.76E-11 ID:SGTC_1847|Compound:st056231|FD-Score:-3.91|P-value:5.22E-5 ID:SGTC_1854|Compound:st056233|FD-Score:3.18|P-value:3.47E-4 ID:SGTC_1855|Compound:st056195|FD-Score:3.11|P-value:4.42E-4 ID:SGTC_1923|Compound:st058458|FD-Score:4.08|P-value:7.94E-6 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.15|P-value:1.81E-5 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:3.06|P-value:5.36E-4 ID:SGTC_1934|Compound:st074720|FD-Score:3.25|P-value:2.60E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.36|P-value:1.76E-4 ID:SGTC_2113|Compound:5491891|FD-Score:-3.14|P-value:9.83E-4 ID:SGTC_2124|Compound:5246320|FD-Score:4.42|P-value:1.53E-6 ID:SGTC_2290|Compound:7904846|FD-Score:3.07|P-value:5.25E-4 ID:SGTC_2318|Compound:6029776|FD-Score:3.16|P-value:3.76E-4 ID:SGTC_2452|Compound:5863136|FD-Score:3.13|P-value:4.15E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:2.93|P-value:8.57E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:5.13|P-value:3.51E-8 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:3.44|P-value:1.26E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:4.61|P-value:5.71E-7 ID:SGTC_2586|Compound:curcumin|FD-Score:4.12|P-value:6.50E-6 ID:SGTC_2846|Compound:9008642|FD-Score:3.21|P-value:3.08E-4 ID:SGTC_2859|Compound:9028643|FD-Score:3.62|P-value:6.13E-5 ID:SGTC_2950|Compound:9072895|FD-Score:3.75|P-value:3.47E-5 ID:SGTC_2975|Compound:9084847|FD-Score:-3.74|P-value:1.03E-4 ID:SGTC_2981|Compound:9013952|FD-Score:4.60|P-value:6.11E-7 ID:SGTC_2986|Compound:9022445|FD-Score:10.30|P-value:7.69E-27 ID:SGTC_3071|Compound:9117417|FD-Score:5.54|P-value:2.93E-9 ID:SGTC_3073|Compound:9114972|FD-Score:4.52|P-value:9.04E-7 ID:SGTC_3074|Compound:9116018|FD-Score:-4.35|P-value:7.43E-6 ID:SGTC_3104|Compound:9120930|FD-Score:-3.32|P-value:5.35E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.25|P-value:2.68E-4 ID:SGTC_3232|Compound:9132874|FD-Score:5.15|P-value:3.03E-8 ID:SGTC_3277|Compound:9138724|FD-Score:3.01|P-value:6.53E-4

Top fitness defect scores for YKL017C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2986 9022445 10.30 71.4 µM 7.69E-27 1 5.21E-25
2 SGTC_1845 st056753 6.09 3.0 µM 8.76E-11 TSC3-RPN4 10 5.60E-10
3 SGTC_3071 9117417 5.54 49.5 µM 2.93E-9 fatty acid desaturase (OLE1) 6 1.47E-8
4 SGTC_3232 9132874 5.15 49.5 µM 3.03E-8 RPP1 & pyrimidine depletion 5 1.30E-7
5 SGTC_2532 xanthone 5.13 78.9 µM 3.51E-8 15 1.48E-7
6 SGTC_910 3454-3003 5.05 490.0 µM 5.54E-8 4 2.27E-7
7 SGTC_1779 st039427 4.97 55.0 µM 8.45E-8 7 3.36E-7
8 SGTC_2541 muurolladie-3-one 4.61 83.1 µM 5.71E-7 excess fatty acid 14 1.98E-6
9 SGTC_2981 9013952 4.60 39.0 µM 6.11E-7 28 2.11E-6
10 SGTC_3073 9114972 4.52 49.5 µM 9.04E-7 60S ribosome export 5 3.03E-6
11 SGTC_2124 5246320 4.42 200.0 µM 1.53E-6 heme biosynthesis & mitochondrial translocase 12 4.93E-6
12 SGTC_2586 curcumin 4.12 80.0 µM 6.50E-6 iron homeostasis 44 1.89E-5
13 SGTC_1923 st058458 4.08 19.1 µM 7.94E-6 30 2.27E-5
14 SGTC_343 4130-2827 4.02 29.8 µM 1.02E-5 30 2.85E-5
15 SGTC_621 3584-0052 3.94 32.1 µM 1.51E-5 28 4.11E-5
16 SGTC_1371 2421-0008 3.88 32.7 µM 1.96E-5 mitochondrial processes 43 5.24E-5
17 SGTC_1704 st028805 3.85 70.8 µM 2.19E-5 12 5.79E-5
18 SGTC_865 0488-0499 3.81 301.0 µM 2.63E-5 20 6.88E-5
19 SGTC_2950 9072895 3.75 21.5 µM 3.47E-5 47 8.86E-5
20 SGTC_1285 0929-0069 3.74 65.5 µM 3.58E-5 23 9.12E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.147 1.42E-17 YFL044C OTU1 hom Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress
0.115 2.07E-11 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.108 3.10E-10 YER148W SPT15 het TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
0.108 4.06E-10 YHL007C STE20 hom Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p
0.103 2.58E-9 YBL050W SEC17 het Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP
0.098 1.13E-8 YDR421W ARO80 hom Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids
0.090 1.71E-7 YMR167W MLH1 hom Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer
0.089 2.74E-7 YHR123W EPT1 hom sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication
0.088 3.34E-7 YMR020W FMS1 hom Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis
0.087 3.99E-7 YDR210W_p YDR210W_p hom Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.085 7.26E-7 YGR154C GTO1 hom Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization
0.084 1.01E-6 YDR286C_p YDR286C_p hom Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site
0.084 1.27E-6 YOR302W YOR302W hom CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA
0.083 1.50E-6 YKL147C_d YKL147C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3
0.083 1.66E-6 YFL052W_p YFL052W_p hom Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity