YKL019W / RAM2

Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL019W / RAM2

Click on Significant Values for Screen Details ID:SGTC_224|Compound:benfluorex|FD-Score:2.95|P-value:4.00E-4 ID:SGTC_347|Compound:4534-1311|FD-Score:-4.40|P-value:3.78E-7 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:-4.32|P-value:6.19E-7 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:-3.41|P-value:6.50E-5 ID:SGTC_484|Compound:loperamide|FD-Score:-2.76|P-value:9.89E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:2.74|P-value:9.23E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:2.73|P-value:9.50E-4 ID:SGTC_1012|Compound:4112-3522|FD-Score:-2.96|P-value:4.60E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:-4.68|P-value:7.09E-8 ID:SGTC_1093|Compound:fluphenazine|FD-Score:4.06|P-value:2.05E-6 ID:SGTC_1097|Compound:itavastatin|FD-Score:-3.37|P-value:7.65E-5 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.94|P-value:3.83E-6 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.80|P-value:9.71E-6 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:-2.95|P-value:4.76E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:2.80|P-value:7.31E-4 ID:SGTC_1144|Compound:3486-0183|FD-Score:-2.76|P-value:9.87E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.23|P-value:1.24E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:3.30|P-value:9.06E-5 ID:SGTC_1280|Compound:0880-0253|FD-Score:2.86|P-value:5.68E-4 ID:SGTC_1391|Compound:3442-0225|FD-Score:3.45|P-value:4.53E-5 ID:SGTC_1721|Compound:st082152|FD-Score:-3.09|P-value:2.67E-4 ID:SGTC_1808|Compound:st050063|FD-Score:3.49|P-value:3.74E-5 ID:SGTC_1810|Compound:st050138|FD-Score:3.49|P-value:3.78E-5 ID:SGTC_1815|Compound:st051655|FD-Score:3.40|P-value:5.66E-5 ID:SGTC_1822|Compound:st052468|FD-Score:-3.04|P-value:3.28E-4 ID:SGTC_2108|Compound:5365811|FD-Score:-2.87|P-value:6.53E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:2.88|P-value:5.25E-4 ID:SGTC_2177|Compound:5811945|FD-Score:3.46|P-value:4.36E-5 ID:SGTC_2297|Compound:7971671|FD-Score:2.77|P-value:8.24E-4 ID:SGTC_2416|Compound:tofa|FD-Score:-2.95|P-value:4.64E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:2.75|P-value:8.93E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:5.66|P-value:7.09E-11 ID:SGTC_2600|Compound:tangeritin|FD-Score:-3.60|P-value:2.66E-5 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.00|P-value:3.33E-4 ID:SGTC_2798|Compound:7813637|FD-Score:4.55|P-value:1.22E-7 ID:SGTC_2834|Compound:9001755|FD-Score:-3.22|P-value:1.50E-4 ID:SGTC_2885|Compound:9057732|FD-Score:2.78|P-value:7.77E-4 ID:SGTC_2963|Compound:9082507|FD-Score:2.98|P-value:3.50E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.99|P-value:1.01E-8 ID:SGTC_3076|Compound:9116187|FD-Score:-2.79|P-value:8.85E-4 ID:SGTC_3082|Compound:9117008|FD-Score:-3.04|P-value:3.25E-4 ID:SGTC_3083|Compound:9117465|FD-Score:-2.83|P-value:7.55E-4 ID:SGTC_3120|Compound:9124077|FD-Score:31.30|P-value:8.92E-274 ID:SGTC_3127|Compound:9126946|FD-Score:-2.92|P-value:5.36E-4 ID:SGTC_3288|Compound:9119690|FD-Score:27.10|P-value:2.87E-206 ID:SGTC_3304|Compound:9125678|FD-Score:42.40|P-value:0 ID:SGTC_224|Compound:benfluorex|FD-Score:2.95|P-value:4.00E-4 ID:SGTC_347|Compound:4534-1311|FD-Score:-4.40|P-value:3.78E-7 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:-4.32|P-value:6.19E-7 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:-3.41|P-value:6.50E-5 ID:SGTC_484|Compound:loperamide|FD-Score:-2.76|P-value:9.89E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:2.74|P-value:9.23E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:2.73|P-value:9.50E-4 ID:SGTC_1012|Compound:4112-3522|FD-Score:-2.96|P-value:4.60E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:-4.68|P-value:7.09E-8 ID:SGTC_1093|Compound:fluphenazine|FD-Score:4.06|P-value:2.05E-6 ID:SGTC_1097|Compound:itavastatin|FD-Score:-3.37|P-value:7.65E-5 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.94|P-value:3.83E-6 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.80|P-value:9.71E-6 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:-2.95|P-value:4.76E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:2.80|P-value:7.31E-4 ID:SGTC_1144|Compound:3486-0183|FD-Score:-2.76|P-value:9.87E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.23|P-value:1.24E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:3.30|P-value:9.06E-5 ID:SGTC_1280|Compound:0880-0253|FD-Score:2.86|P-value:5.68E-4 ID:SGTC_1391|Compound:3442-0225|FD-Score:3.45|P-value:4.53E-5 ID:SGTC_1721|Compound:st082152|FD-Score:-3.09|P-value:2.67E-4 ID:SGTC_1808|Compound:st050063|FD-Score:3.49|P-value:3.74E-5 ID:SGTC_1810|Compound:st050138|FD-Score:3.49|P-value:3.78E-5 ID:SGTC_1815|Compound:st051655|FD-Score:3.40|P-value:5.66E-5 ID:SGTC_1822|Compound:st052468|FD-Score:-3.04|P-value:3.28E-4 ID:SGTC_2108|Compound:5365811|FD-Score:-2.87|P-value:6.53E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:2.88|P-value:5.25E-4 ID:SGTC_2177|Compound:5811945|FD-Score:3.46|P-value:4.36E-5 ID:SGTC_2297|Compound:7971671|FD-Score:2.77|P-value:8.24E-4 ID:SGTC_2416|Compound:tofa|FD-Score:-2.95|P-value:4.64E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:2.75|P-value:8.93E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:5.66|P-value:7.09E-11 ID:SGTC_2600|Compound:tangeritin|FD-Score:-3.60|P-value:2.66E-5 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.00|P-value:3.33E-4 ID:SGTC_2798|Compound:7813637|FD-Score:4.55|P-value:1.22E-7 ID:SGTC_2834|Compound:9001755|FD-Score:-3.22|P-value:1.50E-4 ID:SGTC_2885|Compound:9057732|FD-Score:2.78|P-value:7.77E-4 ID:SGTC_2963|Compound:9082507|FD-Score:2.98|P-value:3.50E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.99|P-value:1.01E-8 ID:SGTC_3076|Compound:9116187|FD-Score:-2.79|P-value:8.85E-4 ID:SGTC_3082|Compound:9117008|FD-Score:-3.04|P-value:3.25E-4 ID:SGTC_3083|Compound:9117465|FD-Score:-2.83|P-value:7.55E-4 ID:SGTC_3120|Compound:9124077|FD-Score:31.30|P-value:8.92E-274 ID:SGTC_3127|Compound:9126946|FD-Score:-2.92|P-value:5.36E-4 ID:SGTC_3288|Compound:9119690|FD-Score:27.10|P-value:2.87E-206 ID:SGTC_3304|Compound:9125678|FD-Score:42.40|P-value:0

Top fitness defect scores for YKL019W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3304 9125678 42.40 46.1 µM 0 2 0 10.90
2 SGTC_3120 9124077 31.30 49.5 µM 8.92E-274 2 3.64E-215 12.50
3 SGTC_3288 9119690 27.10 36.2 µM 2.87E-206 1 3.39E-162 7.82
4 SGTC_2563 tomatine 5.66 8.5 µM 7.09E-11 6 7.43E-9 0.43
5 SGTC_2798 7813637 4.55 45.5 µM 1.22E-7 11 2.63E-6 0.49
6 SGTC_1093 fluphenazine 4.06 8.0 µM 2.05E-6 3 2.43E-5 0.03
7 SGTC_1104 tacrolimus 3.94 29.8 µM 3.83E-6 9 3.99E-5 0.31
8 SGTC_1808 st050063 3.49 61.5 µM 3.74E-5 2 2.42E-4 0.19
9 SGTC_1810 st050138 3.49 53.0 µM 3.78E-5 PDR1 5 2.44E-4 0.04
10 SGTC_2177 5811945 3.46 192.0 µM 4.36E-5 5 2.73E-4 0.07
11 SGTC_1391 3442-0225 3.45 151.0 µM 4.53E-5 6 2.82E-4 0.02
12 SGTC_1815 st051655 3.40 58.2 µM 5.66E-5 TSC3-RPN4 22 3.36E-4 0.08
13 SGTC_1172 0370-0043 3.30 85.0 µM 9.06E-5 10 4.88E-4 0.10
14 SGTC_1150 3909-7983 3.23 10.9 µM 1.24E-4 17 6.24E-4 0.08
15 SGTC_2757 clomiphene 3.00 4.2 µM 3.33E-4 fatty acid desaturase (OLE1) 32 0.00137 0.06
16 SGTC_2963 9082507 2.98 9.0 µM 3.50E-4 21 0.00142 0.04
17 SGTC_224 benfluorex 2.95 43.9 µM 4.00E-4 14 0.00158 0.18
18 SGTC_2131 toluylene red 2.88 5.3 µM 5.25E-4 60S ribosome export 30 0.00197 0.02
19 SGTC_1280 0880-0253 2.86 10.1 µM 5.68E-4 10 0.00209 0.11
20 SGTC_1137 ixabepilone 2.80 100.0 µM 7.31E-4 17 0.00256 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.902 0 YGL155W CDC43 het Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
0.652 0 YLR230W_d YLR230W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
0.579 1.95E-299 YLR229C CDC42 het Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins
0.537 5.92E-250 YKL058W TOA2 het TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
0.483 1.63E-195 YLR153C ACS2 het Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
0.341 4.56E-92 YDR309C GIC2 hom Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region
0.285 6.86E-64 YML017W PSP2 hom Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing
0.230 1.94E-41 YMR033W ARP9 het Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
0.224 1.46E-39 YPR034W ARP7 het Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
0.222 2.01E-38 YDL090C RAM1 hom Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
0.219 9.45E-38 YJL167W ERG20 het Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis
0.197 9.61E-31 YGL049C TIF4632 hom Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication
0.189 3.60E-28 YAL066W_d YAL066W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.182 2.34E-26 YGL154C LYS5 hom Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine
0.177 5.13E-25 YMR068W AVO2 hom Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth