YKL020C / SPT23

ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL020C / SPT23

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:-3.31|P-value:1.93E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:6.86|P-value:9.84E-14 ID:SGTC_105|Compound:0111-0055|FD-Score:3.38|P-value:1.49E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:3.89|P-value:1.51E-5 ID:SGTC_121|Compound:0557-0495|FD-Score:-3.75|P-value:2.84E-5 ID:SGTC_266|Compound:formosanin C|FD-Score:4.77|P-value:1.63E-7 ID:SGTC_373|Compound:0844-0104|FD-Score:3.20|P-value:3.00E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:-3.33|P-value:1.78E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.84|P-value:1.92E-5 ID:SGTC_531|Compound:3788-1495|FD-Score:3.01|P-value:6.22E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:3.60|P-value:5.76E-5 ID:SGTC_700|Compound:3453-2283|FD-Score:-3.53|P-value:7.57E-5 ID:SGTC_704|Compound:1310-0270|FD-Score:-4.04|P-value:7.30E-6 ID:SGTC_723|Compound:4182-0001|FD-Score:4.39|P-value:1.26E-6 ID:SGTC_834|Compound:0312-0008|FD-Score:4.28|P-value:2.29E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:3.90|P-value:1.48E-5 ID:SGTC_935|Compound:2996-0153|FD-Score:4.67|P-value:2.83E-7 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:2.98|P-value:6.97E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:4.37|P-value:1.42E-6 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:-3.42|P-value:1.23E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:-3.34|P-value:1.69E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.41|P-value:1.15E-6 ID:SGTC_1358|Compound:1509-0022|FD-Score:3.14|P-value:3.77E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:2.95|P-value:7.78E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:3.85|P-value:1.85E-5 ID:SGTC_1500|Compound:4488-1866|FD-Score:5.18|P-value:1.45E-8 ID:SGTC_1694|Compound:st024011|FD-Score:3.27|P-value:2.29E-4 ID:SGTC_1706|Compound:st031321|FD-Score:2.99|P-value:6.65E-4 ID:SGTC_1715|Compound:st032160|FD-Score:3.70|P-value:3.70E-5 ID:SGTC_1835|Compound:st008666|FD-Score:3.04|P-value:5.60E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.44|P-value:1.15E-4 ID:SGTC_1941|Compound:st076221|FD-Score:7.08|P-value:1.61E-14 ID:SGTC_1994|Compound:st072947|FD-Score:3.88|P-value:1.60E-5 ID:SGTC_2035|Compound:5154463|FD-Score:4.27|P-value:2.43E-6 ID:SGTC_2069|Compound:5228469|FD-Score:3.01|P-value:6.23E-4 ID:SGTC_2112|Compound:5488546|FD-Score:3.74|P-value:3.04E-5 ID:SGTC_2239|Compound:6685329|FD-Score:-3.90|P-value:1.41E-5 ID:SGTC_2303|Compound:7774051|FD-Score:-3.08|P-value:4.73E-4 ID:SGTC_2446|Compound:5162541|FD-Score:2.92|P-value:8.66E-4 ID:SGTC_2517|Compound:totarol|FD-Score:3.16|P-value:3.49E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.62|P-value:5.12E-5 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:4.46|P-value:8.83E-7 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:3.50|P-value:8.90E-5 ID:SGTC_2607|Compound:solasodine|FD-Score:4.27|P-value:2.36E-6 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.16|P-value:3.49E-4 ID:SGTC_2618|Compound:avocadyne|FD-Score:-2.90|P-value:9.39E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:3.37|P-value:1.50E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:4.10|P-value:5.43E-6 ID:SGTC_2780|Compound:5215298|FD-Score:2.92|P-value:8.61E-4 ID:SGTC_2788|Compound:5282734|FD-Score:4.07|P-value:6.32E-6 ID:SGTC_2797|Compound:7859098|FD-Score:3.10|P-value:4.50E-4 ID:SGTC_2851|Compound:9015922|FD-Score:-3.94|P-value:1.17E-5 ID:SGTC_2858|Compound:9025781|FD-Score:3.91|P-value:1.41E-5 ID:SGTC_2868|Compound:9035608|FD-Score:-3.26|P-value:2.38E-4 ID:SGTC_2874|Compound:9043904|FD-Score:5.01|P-value:3.84E-8 ID:SGTC_2878|Compound:9043973|FD-Score:3.03|P-value:5.92E-4 ID:SGTC_2991|Compound:9074669|FD-Score:6.21|P-value:1.36E-11 ID:SGTC_2997|Compound:9067075|FD-Score:4.19|P-value:3.64E-6 ID:SGTC_3048|Compound:9093155|FD-Score:4.58|P-value:4.49E-7 ID:SGTC_3060|Compound:1327-0027|FD-Score:3.46|P-value:1.06E-4 ID:SGTC_3203|Compound:9113543|FD-Score:6.17|P-value:1.84E-11 ID:SGTC_3204|Compound:9114183|FD-Score:4.68|P-value:2.71E-7 ID:SGTC_3208|Compound:9114115|FD-Score:-3.47|P-value:9.88E-5 ID:SGTC_3210|Compound:9128157|FD-Score:5.49|P-value:2.00E-9 ID:SGTC_3212|Compound:9128307|FD-Score:-4.36|P-value:1.43E-6 ID:SGTC_3304|Compound:9125678|FD-Score:4.77|P-value:1.63E-7 ID:SGTC_43|Compound:0570-0105|FD-Score:-3.31|P-value:1.93E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:6.86|P-value:9.84E-14 ID:SGTC_105|Compound:0111-0055|FD-Score:3.38|P-value:1.49E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:3.89|P-value:1.51E-5 ID:SGTC_121|Compound:0557-0495|FD-Score:-3.75|P-value:2.84E-5 ID:SGTC_266|Compound:formosanin C|FD-Score:4.77|P-value:1.63E-7 ID:SGTC_373|Compound:0844-0104|FD-Score:3.20|P-value:3.00E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:-3.33|P-value:1.78E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.84|P-value:1.92E-5 ID:SGTC_531|Compound:3788-1495|FD-Score:3.01|P-value:6.22E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:3.60|P-value:5.76E-5 ID:SGTC_700|Compound:3453-2283|FD-Score:-3.53|P-value:7.57E-5 ID:SGTC_704|Compound:1310-0270|FD-Score:-4.04|P-value:7.30E-6 ID:SGTC_723|Compound:4182-0001|FD-Score:4.39|P-value:1.26E-6 ID:SGTC_834|Compound:0312-0008|FD-Score:4.28|P-value:2.29E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:3.90|P-value:1.48E-5 ID:SGTC_935|Compound:2996-0153|FD-Score:4.67|P-value:2.83E-7 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:2.98|P-value:6.97E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:4.37|P-value:1.42E-6 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:-3.42|P-value:1.23E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:-3.34|P-value:1.69E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.41|P-value:1.15E-6 ID:SGTC_1358|Compound:1509-0022|FD-Score:3.14|P-value:3.77E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:2.95|P-value:7.78E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:3.85|P-value:1.85E-5 ID:SGTC_1500|Compound:4488-1866|FD-Score:5.18|P-value:1.45E-8 ID:SGTC_1694|Compound:st024011|FD-Score:3.27|P-value:2.29E-4 ID:SGTC_1706|Compound:st031321|FD-Score:2.99|P-value:6.65E-4 ID:SGTC_1715|Compound:st032160|FD-Score:3.70|P-value:3.70E-5 ID:SGTC_1835|Compound:st008666|FD-Score:3.04|P-value:5.60E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.44|P-value:1.15E-4 ID:SGTC_1941|Compound:st076221|FD-Score:7.08|P-value:1.61E-14 ID:SGTC_1994|Compound:st072947|FD-Score:3.88|P-value:1.60E-5 ID:SGTC_2035|Compound:5154463|FD-Score:4.27|P-value:2.43E-6 ID:SGTC_2069|Compound:5228469|FD-Score:3.01|P-value:6.23E-4 ID:SGTC_2112|Compound:5488546|FD-Score:3.74|P-value:3.04E-5 ID:SGTC_2239|Compound:6685329|FD-Score:-3.90|P-value:1.41E-5 ID:SGTC_2303|Compound:7774051|FD-Score:-3.08|P-value:4.73E-4 ID:SGTC_2446|Compound:5162541|FD-Score:2.92|P-value:8.66E-4 ID:SGTC_2517|Compound:totarol|FD-Score:3.16|P-value:3.49E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.62|P-value:5.12E-5 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:4.46|P-value:8.83E-7 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:3.50|P-value:8.90E-5 ID:SGTC_2607|Compound:solasodine|FD-Score:4.27|P-value:2.36E-6 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.16|P-value:3.49E-4 ID:SGTC_2618|Compound:avocadyne|FD-Score:-2.90|P-value:9.39E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:3.37|P-value:1.50E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:4.10|P-value:5.43E-6 ID:SGTC_2780|Compound:5215298|FD-Score:2.92|P-value:8.61E-4 ID:SGTC_2788|Compound:5282734|FD-Score:4.07|P-value:6.32E-6 ID:SGTC_2797|Compound:7859098|FD-Score:3.10|P-value:4.50E-4 ID:SGTC_2851|Compound:9015922|FD-Score:-3.94|P-value:1.17E-5 ID:SGTC_2858|Compound:9025781|FD-Score:3.91|P-value:1.41E-5 ID:SGTC_2868|Compound:9035608|FD-Score:-3.26|P-value:2.38E-4 ID:SGTC_2874|Compound:9043904|FD-Score:5.01|P-value:3.84E-8 ID:SGTC_2878|Compound:9043973|FD-Score:3.03|P-value:5.92E-4 ID:SGTC_2991|Compound:9074669|FD-Score:6.21|P-value:1.36E-11 ID:SGTC_2997|Compound:9067075|FD-Score:4.19|P-value:3.64E-6 ID:SGTC_3048|Compound:9093155|FD-Score:4.58|P-value:4.49E-7 ID:SGTC_3060|Compound:1327-0027|FD-Score:3.46|P-value:1.06E-4 ID:SGTC_3203|Compound:9113543|FD-Score:6.17|P-value:1.84E-11 ID:SGTC_3204|Compound:9114183|FD-Score:4.68|P-value:2.71E-7 ID:SGTC_3208|Compound:9114115|FD-Score:-3.47|P-value:9.88E-5 ID:SGTC_3210|Compound:9128157|FD-Score:5.49|P-value:2.00E-9 ID:SGTC_3212|Compound:9128307|FD-Score:-4.36|P-value:1.43E-6 ID:SGTC_3304|Compound:9125678|FD-Score:4.77|P-value:1.63E-7

Top fitness defect scores for YKL020C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1941 st076221 7.08 61.8 µM 1.61E-14 sphingolipid biosynthesis & PDR1 26 7.18E-13
2 SGTC_103 0844-0104 6.86 51.8 µM 9.84E-14 ERAD & cell cycle 62 3.49E-12
3 SGTC_2991 9074669 6.21 71.4 µM 1.36E-11 azole & statin 19 2.57E-10
4 SGTC_3203 9113543 6.17 49.5 µM 1.84E-11 4 3.35E-10
5 SGTC_3210 9128157 5.49 49.5 µM 2.00E-9 19 2.01E-8
6 SGTC_1500 4488-1866 5.18 171.0 µM 1.45E-8 RPP1 & pyrimidine depletion 4 1.13E-7
7 SGTC_2874 9043904 5.01 71.4 µM 3.84E-8 2 2.66E-7
8 SGTC_3304 9125678 4.77 46.1 µM 1.63E-7 26 9.39E-7
9 SGTC_266 formosanin C 4.77 406.0 nM 1.63E-7 sphingolipid biosynthesis & PDR1 51 9.42E-7
10 SGTC_3204 9114183 4.68 49.5 µM 2.71E-7 16 1.47E-6
11 SGTC_935 2996-0153 4.67 430.0 µM 2.83E-7 PDR1 10 1.52E-6
12 SGTC_3048 9093155 4.58 49.5 µM 4.49E-7 2 2.28E-6
13 SGTC_2598 perillyl alcohol 4.46 100.0 µM 8.83E-7 19 4.13E-6
14 SGTC_1161 k007-0610 4.41 110.0 µM 1.15E-6 ERAD & cell cycle 100 5.19E-6
15 SGTC_723 4182-0001 4.39 115.0 µM 1.26E-6 22 5.63E-6
16 SGTC_1087 pterostilbene 4.37 9.1 µM 1.42E-6 18 6.25E-6
17 SGTC_834 0312-0008 4.28 75.7 µM 2.29E-6 NEO1 25 9.51E-6
18 SGTC_2607 solasodine 4.27 47.4 µM 2.36E-6 49 9.74E-6
19 SGTC_2035 5154463 4.27 94.6 µM 2.43E-6 10 9.99E-6
20 SGTC_2997 9067075 4.19 71.4 µM 3.64E-6 unfolded protein response 49 1.42E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.182 2.52E-26 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.180 7.52E-26 YPL117C IDI1 het Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability
0.143 6.76E-17 YGR143W SKN1 hom Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p
0.142 1.36E-16 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.142 1.39E-16 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.142 1.67E-16 YMR029C FAR8 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.141 2.17E-16 YKL058W TOA2 het TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
0.140 4.28E-16 YOL065C INP54 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.139 6.06E-16 YNL257C SIP3 hom Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication
0.132 1.91E-14 YNL229C URE2 hom Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
0.131 2.46E-14 YDR123C INO2 hom Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion
0.131 2.70E-14 YDR326C YSP2 hom Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication
0.131 2.82E-14 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.128 8.79E-14 YBL079W NUP170 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
0.128 9.68E-14 YKR014C YPT52 hom Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress