YKL021C / MAK11

Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL021C / MAK11

Click on Significant Values for Screen Details ID:SGTC_73|Compound:0327-0325|FD-Score:-3.08|P-value:4.46E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:-2.96|P-value:6.85E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:5.67|P-value:7.57E-10 ID:SGTC_610|Compound:4215-0184|FD-Score:3.37|P-value:1.71E-4 ID:SGTC_703|Compound:3875-0075|FD-Score:2.99|P-value:7.43E-4 ID:SGTC_739|Compound:1486-1183|FD-Score:-3.01|P-value:5.68E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:3.65|P-value:5.23E-5 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:4.14|P-value:5.25E-6 ID:SGTC_1122|Compound:capsaicin|FD-Score:4.04|P-value:8.68E-6 ID:SGTC_1199|Compound:1638-0010|FD-Score:3.41|P-value:1.45E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:-3.01|P-value:5.75E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:3.06|P-value:5.81E-4 ID:SGTC_1632|Compound:st005650|FD-Score:4.05|P-value:8.26E-6 ID:SGTC_1940|Compound:st075990|FD-Score:4.09|P-value:6.66E-6 ID:SGTC_2077|Compound:5212015|FD-Score:3.09|P-value:5.28E-4 ID:SGTC_2079|Compound:5212404|FD-Score:5.77|P-value:3.89E-10 ID:SGTC_2162|Compound:5663267|FD-Score:4.59|P-value:5.27E-7 ID:SGTC_2222|Compound:6885208|FD-Score:4.34|P-value:1.91E-6 ID:SGTC_2228|Compound:7354201|FD-Score:3.52|P-value:9.05E-5 ID:SGTC_2263|Compound:7948595|FD-Score:-3.37|P-value:1.36E-4 ID:SGTC_2269|Compound:7935389|FD-Score:-3.95|P-value:1.05E-5 ID:SGTC_2270|Compound:7924575|FD-Score:3.23|P-value:3.05E-4 ID:SGTC_2275|Compound:7947463|FD-Score:3.21|P-value:3.33E-4 ID:SGTC_2282|Compound:7944268|FD-Score:3.22|P-value:3.09E-4 ID:SGTC_2448|Compound:5185112|FD-Score:3.99|P-value:1.10E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:2.94|P-value:9.12E-4 ID:SGTC_2763|Compound:lisinopril|FD-Score:-3.25|P-value:2.30E-4 ID:SGTC_2788|Compound:5282734|FD-Score:3.62|P-value:5.92E-5 ID:SGTC_2894|Compound:9048625|FD-Score:3.80|P-value:2.71E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-5.19|P-value:1.16E-8 ID:SGTC_3138|Compound:9097216|FD-Score:-3.54|P-value:6.83E-5 ID:SGTC_3143|Compound:9086658|FD-Score:7.31|P-value:3.18E-15 ID:SGTC_3193|Compound:9111931|FD-Score:3.88|P-value:1.85E-5 ID:SGTC_3274|Compound:9138298|FD-Score:3.54|P-value:8.39E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.08|P-value:4.46E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:-2.96|P-value:6.85E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:5.67|P-value:7.57E-10 ID:SGTC_610|Compound:4215-0184|FD-Score:3.37|P-value:1.71E-4 ID:SGTC_703|Compound:3875-0075|FD-Score:2.99|P-value:7.43E-4 ID:SGTC_739|Compound:1486-1183|FD-Score:-3.01|P-value:5.68E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:3.65|P-value:5.23E-5 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:4.14|P-value:5.25E-6 ID:SGTC_1122|Compound:capsaicin|FD-Score:4.04|P-value:8.68E-6 ID:SGTC_1199|Compound:1638-0010|FD-Score:3.41|P-value:1.45E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:-3.01|P-value:5.75E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:3.06|P-value:5.81E-4 ID:SGTC_1632|Compound:st005650|FD-Score:4.05|P-value:8.26E-6 ID:SGTC_1940|Compound:st075990|FD-Score:4.09|P-value:6.66E-6 ID:SGTC_2077|Compound:5212015|FD-Score:3.09|P-value:5.28E-4 ID:SGTC_2079|Compound:5212404|FD-Score:5.77|P-value:3.89E-10 ID:SGTC_2162|Compound:5663267|FD-Score:4.59|P-value:5.27E-7 ID:SGTC_2222|Compound:6885208|FD-Score:4.34|P-value:1.91E-6 ID:SGTC_2228|Compound:7354201|FD-Score:3.52|P-value:9.05E-5 ID:SGTC_2263|Compound:7948595|FD-Score:-3.37|P-value:1.36E-4 ID:SGTC_2269|Compound:7935389|FD-Score:-3.95|P-value:1.05E-5 ID:SGTC_2270|Compound:7924575|FD-Score:3.23|P-value:3.05E-4 ID:SGTC_2275|Compound:7947463|FD-Score:3.21|P-value:3.33E-4 ID:SGTC_2282|Compound:7944268|FD-Score:3.22|P-value:3.09E-4 ID:SGTC_2448|Compound:5185112|FD-Score:3.99|P-value:1.10E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:2.94|P-value:9.12E-4 ID:SGTC_2763|Compound:lisinopril|FD-Score:-3.25|P-value:2.30E-4 ID:SGTC_2788|Compound:5282734|FD-Score:3.62|P-value:5.92E-5 ID:SGTC_2894|Compound:9048625|FD-Score:3.80|P-value:2.71E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-5.19|P-value:1.16E-8 ID:SGTC_3138|Compound:9097216|FD-Score:-3.54|P-value:6.83E-5 ID:SGTC_3143|Compound:9086658|FD-Score:7.31|P-value:3.18E-15 ID:SGTC_3193|Compound:9111931|FD-Score:3.88|P-value:1.85E-5 ID:SGTC_3274|Compound:9138298|FD-Score:3.54|P-value:8.39E-5

Top fitness defect scores for YKL021C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3143 9086658 7.31 49.5 µM 3.18E-15 1 1.34E-13 0.78
2 SGTC_2079 5212404 5.77 121.0 µM 3.89E-10 1 3.95E-9 2.14
3 SGTC_423 5-fluorouracil 5.67 457.0 µM 7.57E-10 exosome 15 7.09E-9 0.35
4 SGTC_2162 5663267 4.59 200.0 µM 5.27E-7 5 2.26E-6 0.15
5 SGTC_2222 6885208 4.34 172.1 µM 1.91E-6 1 7.02E-6 1.65
6 SGTC_1077 5-fluorocytosine 4.14 377.0 nM 5.25E-6 exosome 17 1.72E-5 0.17
7 SGTC_1940 st075990 4.09 51.1 µM 6.66E-6 60S ribosome export 6 2.11E-5 0.55
8 SGTC_1632 st005650 4.05 90.0 µM 8.26E-6 6 2.56E-5 0.04
9 SGTC_1122 capsaicin 4.04 818.6 nM 8.68E-6 4 2.67E-5 0.23
10 SGTC_2448 5185112 3.99 27.2 µM 1.10E-5 7 3.29E-5 0.47
11 SGTC_3193 9111931 3.88 49.5 µM 1.85E-5 8 5.22E-5 0.01
12 SGTC_2894 9048625 3.80 32.5 µM 2.71E-5 12 7.31E-5 0.12
13 SGTC_1074 carmofur 3.65 3.8 µM 5.23E-5 exosome 30 1.31E-4 0.04
14 SGTC_2788 5282734 3.62 71.4 µM 5.92E-5 14 1.46E-4 0.05
15 SGTC_3274 9138298 3.54 49.5 µM 8.39E-5 7 1.99E-4 0.06
16 SGTC_2228 7354201 3.52 200.0 µM 9.05E-5 endomembrane recycling 3 2.12E-4 0.23
17 SGTC_1199 1638-0010 3.41 4.5 µM 1.45E-4 endomembrane recycling 7 3.22E-4 0.05
18 SGTC_610 4215-0184 3.37 155.0 µM 1.71E-4 exosome 29 3.74E-4 0.04
19 SGTC_2270 7924575 3.23 90.9 µM 3.05E-4 RPP1 & pyrimidine depletion 12 6.24E-4 0.01
20 SGTC_2282 7944268 3.22 200.0 µM 3.09E-4 RPP1 & pyrimidine depletion 13 6.30E-4 0.09

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.248 4.13E-48 YKL172W EBP2 het Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.237 4.07E-44 YGR195W SKI6 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.233 1.55E-42 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.215 2.34E-36 YPR142C_d YPR142C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
0.210 6.37E-35 YPL043W NOP4 het Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
0.207 8.97E-34 YDL111C RRP42 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
0.179 1.39E-25 YPR143W RRP15 het Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
0.171 2.17E-23 YGR158C MTR3 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
0.158 2.66E-20 YGR186W TFG1 het TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
0.155 1.51E-19 YOL021C DIS3 het Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
0.147 9.05E-18 YDR060W MAK21 het Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
0.145 2.52E-17 YMR049C ERB1 het Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1
0.145 3.51E-17 YMR288W HSH155 het U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155
0.132 2.20E-14 YDR280W RRP45 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
0.131 2.61E-14 YGL057C GEP7 hom Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies