YKL032C / IXR1

Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL032C / IXR1

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:-3.31|P-value:5.45E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:5.58|P-value:7.86E-8 ID:SGTC_72|Compound:0744-0168|FD-Score:5.87|P-value:1.71E-8 ID:SGTC_76|Compound:0433-0009|FD-Score:-5.60|P-value:2.34E-8 ID:SGTC_109|Compound:0132-0036|FD-Score:3.42|P-value:7.46E-4 ID:SGTC_133|Compound:0724-0033|FD-Score:3.58|P-value:4.30E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:9.63|P-value:3.78E-20 ID:SGTC_142|Compound:0335-0849|FD-Score:6.18|P-value:2.88E-9 ID:SGTC_230|Compound:MPP+|FD-Score:4.77|P-value:3.92E-6 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.95|P-value:1.16E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.53|P-value:2.48E-4 ID:SGTC_606|Compound:k015-0008|FD-Score:3.90|P-value:1.39E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:3.39|P-value:8.19E-4 ID:SGTC_638|Compound:3970-0837|FD-Score:4.35|P-value:2.36E-5 ID:SGTC_738|Compound:1315-0003|FD-Score:3.63|P-value:3.60E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:7.11|P-value:9.93E-12 ID:SGTC_836|Compound:0330-0104|FD-Score:3.84|P-value:1.75E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:-3.54|P-value:2.42E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:3.62|P-value:3.81E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:3.85|P-value:1.70E-4 ID:SGTC_1007|Compound:4101-0031|FD-Score:-3.16|P-value:8.88E-4 ID:SGTC_1017|Compound:4239-0030|FD-Score:3.39|P-value:8.18E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:3.44|P-value:7.10E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:3.84|P-value:1.71E-4 ID:SGTC_1321|Compound:1326-2144|FD-Score:3.50|P-value:5.73E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:3.73|P-value:2.62E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:-3.45|P-value:3.31E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:4.09|P-value:6.85E-5 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.43|P-value:7.20E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.35|P-value:4.76E-4 ID:SGTC_1675|Compound:st018485|FD-Score:6.92|P-value:3.43E-11 ID:SGTC_1708|Compound:st033234|FD-Score:3.64|P-value:3.59E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.91|P-value:6.18E-5 ID:SGTC_1728|Compound:st035614|FD-Score:-3.61|P-value:1.86E-4 ID:SGTC_1776|Compound:st049249|FD-Score:4.65|P-value:6.70E-6 ID:SGTC_1820|Compound:st053765|FD-Score:3.48|P-value:6.05E-4 ID:SGTC_1986|Compound:st071812|FD-Score:3.37|P-value:8.82E-4 ID:SGTC_1999|Compound:st074831|FD-Score:3.65|P-value:3.45E-4 ID:SGTC_2075|Compound:5212009|FD-Score:4.28|P-value:3.22E-5 ID:SGTC_2342|Compound:7990637|FD-Score:4.02|P-value:8.76E-5 ID:SGTC_2407|Compound:paclitaxel|FD-Score:7.99|P-value:2.13E-14 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.54|P-value:4.91E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:3.83|P-value:1.80E-4 ID:SGTC_2560|Compound:caryophyllene|FD-Score:-3.86|P-value:7.46E-5 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:4.14|P-value:5.59E-5 ID:SGTC_2593|Compound:nobiletin|FD-Score:4.31|P-value:2.79E-5 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:6.25|P-value:1.98E-9 ID:SGTC_2610|Compound:ononetin|FD-Score:-5.46|P-value:4.98E-8 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:4.33|P-value:2.54E-5 ID:SGTC_2716|Compound:st080858|FD-Score:3.52|P-value:5.27E-4 ID:SGTC_2717|Compound:st081029|FD-Score:4.78|P-value:3.77E-6 ID:SGTC_2806|Compound:7952793|FD-Score:-3.18|P-value:8.22E-4 ID:SGTC_2807|Compound:7945238|FD-Score:3.48|P-value:6.09E-4 ID:SGTC_2814|Compound:7977482|FD-Score:5.07|P-value:9.67E-7 ID:SGTC_2862|Compound:9027634|FD-Score:5.85|P-value:1.89E-8 ID:SGTC_2908|Compound:7930301|FD-Score:3.66|P-value:3.27E-4 ID:SGTC_2950|Compound:9072895|FD-Score:3.88|P-value:1.51E-4 ID:SGTC_2960|Compound:9079389|FD-Score:4.98|P-value:1.53E-6 ID:SGTC_3164|Compound:9100705|FD-Score:3.45|P-value:6.72E-4 ID:SGTC_3226|Compound:9131256|FD-Score:3.35|P-value:9.54E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.54|P-value:5.07E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:-3.31|P-value:5.45E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:5.58|P-value:7.86E-8 ID:SGTC_72|Compound:0744-0168|FD-Score:5.87|P-value:1.71E-8 ID:SGTC_76|Compound:0433-0009|FD-Score:-5.60|P-value:2.34E-8 ID:SGTC_109|Compound:0132-0036|FD-Score:3.42|P-value:7.46E-4 ID:SGTC_133|Compound:0724-0033|FD-Score:3.58|P-value:4.30E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:9.63|P-value:3.78E-20 ID:SGTC_142|Compound:0335-0849|FD-Score:6.18|P-value:2.88E-9 ID:SGTC_230|Compound:MPP+|FD-Score:4.77|P-value:3.92E-6 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.95|P-value:1.16E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.53|P-value:2.48E-4 ID:SGTC_606|Compound:k015-0008|FD-Score:3.90|P-value:1.39E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:3.39|P-value:8.19E-4 ID:SGTC_638|Compound:3970-0837|FD-Score:4.35|P-value:2.36E-5 ID:SGTC_738|Compound:1315-0003|FD-Score:3.63|P-value:3.60E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:7.11|P-value:9.93E-12 ID:SGTC_836|Compound:0330-0104|FD-Score:3.84|P-value:1.75E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:-3.54|P-value:2.42E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:3.62|P-value:3.81E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:3.85|P-value:1.70E-4 ID:SGTC_1007|Compound:4101-0031|FD-Score:-3.16|P-value:8.88E-4 ID:SGTC_1017|Compound:4239-0030|FD-Score:3.39|P-value:8.18E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:3.44|P-value:7.10E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:3.84|P-value:1.71E-4 ID:SGTC_1321|Compound:1326-2144|FD-Score:3.50|P-value:5.73E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:3.73|P-value:2.62E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:-3.45|P-value:3.31E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:4.09|P-value:6.85E-5 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.43|P-value:7.20E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.35|P-value:4.76E-4 ID:SGTC_1675|Compound:st018485|FD-Score:6.92|P-value:3.43E-11 ID:SGTC_1708|Compound:st033234|FD-Score:3.64|P-value:3.59E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.91|P-value:6.18E-5 ID:SGTC_1728|Compound:st035614|FD-Score:-3.61|P-value:1.86E-4 ID:SGTC_1776|Compound:st049249|FD-Score:4.65|P-value:6.70E-6 ID:SGTC_1820|Compound:st053765|FD-Score:3.48|P-value:6.05E-4 ID:SGTC_1986|Compound:st071812|FD-Score:3.37|P-value:8.82E-4 ID:SGTC_1999|Compound:st074831|FD-Score:3.65|P-value:3.45E-4 ID:SGTC_2075|Compound:5212009|FD-Score:4.28|P-value:3.22E-5 ID:SGTC_2342|Compound:7990637|FD-Score:4.02|P-value:8.76E-5 ID:SGTC_2407|Compound:paclitaxel|FD-Score:7.99|P-value:2.13E-14 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.54|P-value:4.91E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:3.83|P-value:1.80E-4 ID:SGTC_2560|Compound:caryophyllene|FD-Score:-3.86|P-value:7.46E-5 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:4.14|P-value:5.59E-5 ID:SGTC_2593|Compound:nobiletin|FD-Score:4.31|P-value:2.79E-5 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:6.25|P-value:1.98E-9 ID:SGTC_2610|Compound:ononetin|FD-Score:-5.46|P-value:4.98E-8 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:4.33|P-value:2.54E-5 ID:SGTC_2716|Compound:st080858|FD-Score:3.52|P-value:5.27E-4 ID:SGTC_2717|Compound:st081029|FD-Score:4.78|P-value:3.77E-6 ID:SGTC_2806|Compound:7952793|FD-Score:-3.18|P-value:8.22E-4 ID:SGTC_2807|Compound:7945238|FD-Score:3.48|P-value:6.09E-4 ID:SGTC_2814|Compound:7977482|FD-Score:5.07|P-value:9.67E-7 ID:SGTC_2862|Compound:9027634|FD-Score:5.85|P-value:1.89E-8 ID:SGTC_2908|Compound:7930301|FD-Score:3.66|P-value:3.27E-4 ID:SGTC_2950|Compound:9072895|FD-Score:3.88|P-value:1.51E-4 ID:SGTC_2960|Compound:9079389|FD-Score:4.98|P-value:1.53E-6 ID:SGTC_3164|Compound:9100705|FD-Score:3.45|P-value:6.72E-4 ID:SGTC_3226|Compound:9131256|FD-Score:3.35|P-value:9.54E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.54|P-value:5.07E-4

Top fitness defect scores for YKL032C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_138 0850-0259 9.63 40.9 µM 3.78E-20 cell wall 46 3.09E-22
2 SGTC_2407 paclitaxel 7.99 400.0 µM 2.13E-14 5 6.73E-16
3 SGTC_789 1309-1135 7.11 70.6 µM 9.93E-12 ERG2 5 5.91E-13
4 SGTC_1675 st018485 6.92 24.5 µM 3.43E-11 60S ribosome export 3 2.33E-12
5 SGTC_2609 2,3,4-trihydroxy-4'-ethoxybenzophenone 6.25 70.0 µM 1.98E-9 redox potentiating 22 2.06E-10
6 SGTC_142 0335-0849 6.18 62.7 µM 2.88E-9 ubiquinone biosynthesis & proteasome 59 3.11E-10
7 SGTC_72 0744-0168 5.87 198.4 µM 1.71E-8 RPP1 & pyrimidine depletion 7 2.23E-9
8 SGTC_2862 9027634 5.85 71.4 µM 1.89E-8 ERG2 11 2.49E-9
9 SGTC_64 000l-0018 5.58 272.1 µM 7.86E-8 ubiquinone biosynthesis & proteasome 91 1.21E-8
10 SGTC_2814 7977482 5.07 43.0 µM 9.67E-7 19 1.94E-7
11 SGTC_2960 9079389 4.98 6.0 µM 1.53E-6 10 3.23E-7
12 SGTC_2717 st081029 4.78 62.3 µM 3.77E-6 7 8.79E-7
13 SGTC_230 MPP+ 4.77 141.7 µM 3.92E-6 7 9.18E-7
14 SGTC_1776 st049249 4.65 45.9 µM 6.70E-6 8 1.66E-6
15 SGTC_638 3970-0837 4.35 4.1 µM 2.36E-5 26 6.73E-6
16 SGTC_2614 arthonioic acid 4.33 13.4 µM 2.54E-5 16 7.30E-6
17 SGTC_2593 nobiletin 4.31 86.2 µM 2.79E-5 20 8.09E-6
18 SGTC_2075 5212009 4.28 200.0 µM 3.22E-5 5 9.51E-6
19 SGTC_2562 3',7'-dimethoxyflavone 4.14 64.9 µM 5.59E-5 26 1.75E-5
20 SGTC_1409 3909-8004 4.09 61.9 µM 6.85E-5 16 2.20E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.152 9.79E-19 YJL080C SCP160 hom Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
0.151 1.31E-18 YJL193W_p YJL193W_p hom Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
0.146 1.82E-17 YLR097C HRT3 hom Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies
0.141 1.96E-16 YJL155C FBP26 hom Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress
0.126 2.95E-13 YPR064W_d YPR064W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.123 9.55E-13 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.121 2.42E-12 YPR033C HTS1 het Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome
0.118 8.51E-12 YER087C-A_d YER087C-A_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase
0.113 4.55E-11 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.113 4.67E-11 YMR264W CUE1 hom Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication
0.113 5.26E-11 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.113 5.96E-11 YDL183C YDL183C hom Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene
0.110 1.48E-10 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.110 2.11E-10 YGL042C_d YGL042C_d hom Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1
0.106 6.76E-10 YNL105W_d RRT16_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription