YKL034W / TUL1

Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL034W / TUL1

Click on Significant Values for Screen Details ID:SGTC_44|Compound:0929-0055|FD-Score:-6.90|P-value:1.15E-12 ID:SGTC_64|Compound:000l-0018|FD-Score:3.30|P-value:4.09E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:4.18|P-value:1.11E-5 ID:SGTC_174|Compound:3553-0389|FD-Score:-3.15|P-value:6.63E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:-4.69|P-value:9.11E-7 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.10|P-value:8.33E-4 ID:SGTC_235|Compound:0330-0087|FD-Score:3.88|P-value:4.13E-5 ID:SGTC_271|Compound:rotenone|FD-Score:3.13|P-value:7.41E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:3.53|P-value:1.73E-4 ID:SGTC_480|Compound:am 92016|FD-Score:4.12|P-value:1.46E-5 ID:SGTC_501|Compound:nsc-95397|FD-Score:-3.05|P-value:9.64E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:3.34|P-value:3.44E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:4.84|P-value:4.55E-7 ID:SGTC_538|Compound:2922-0838|FD-Score:-4.07|P-value:1.75E-5 ID:SGTC_542|Compound:3381-0244|FD-Score:3.95|P-value:2.98E-5 ID:SGTC_574|Compound:1315-0397|FD-Score:-3.21|P-value:5.46E-4 ID:SGTC_658|Compound:3253-0951|FD-Score:3.44|P-value:2.42E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:4.37|P-value:4.68E-6 ID:SGTC_685|Compound:1278-0033|FD-Score:5.88|P-value:1.17E-9 ID:SGTC_730|Compound:1436-0052|FD-Score:4.73|P-value:7.68E-7 ID:SGTC_758|Compound:0868-0029|FD-Score:-8.99|P-value:3.10E-20 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:3.24|P-value:5.09E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:3.33|P-value:3.64E-4 ID:SGTC_1195|Compound:1502-0147|FD-Score:5.45|P-value:1.54E-8 ID:SGTC_1199|Compound:1638-0010|FD-Score:-3.14|P-value:6.96E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.47|P-value:2.15E-4 ID:SGTC_1337|Compound:1486-1011|FD-Score:4.52|P-value:2.24E-6 ID:SGTC_1388|Compound:3346-4487|FD-Score:3.35|P-value:3.38E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:-3.25|P-value:4.62E-4 ID:SGTC_1419|Compound:3993-2473|FD-Score:-3.57|P-value:1.42E-4 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.56|P-value:1.50E-4 ID:SGTC_1497|Compound:4476-2978|FD-Score:3.19|P-value:6.07E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:3.83|P-value:4.99E-5 ID:SGTC_1655|Compound:st012546|FD-Score:4.18|P-value:1.10E-5 ID:SGTC_1674|Compound:st016616|FD-Score:3.35|P-value:3.39E-4 ID:SGTC_1688|Compound:st021091|FD-Score:3.46|P-value:2.26E-4 ID:SGTC_1716|Compound:st033144|FD-Score:6.46|P-value:2.61E-11 ID:SGTC_1818|Compound:st052136|FD-Score:3.87|P-value:4.33E-5 ID:SGTC_1888|Compound:azatryptophan|FD-Score:4.23|P-value:8.94E-6 ID:SGTC_2025|Compound:5147754|FD-Score:3.54|P-value:1.63E-4 ID:SGTC_2032|Compound:5185112|FD-Score:3.37|P-value:3.19E-4 ID:SGTC_2038|Compound:4100038|FD-Score:3.17|P-value:6.46E-4 ID:SGTC_2051|Compound:5263410|FD-Score:-3.77|P-value:6.11E-5 ID:SGTC_2075|Compound:5212009|FD-Score:3.48|P-value:2.09E-4 ID:SGTC_2154|Compound:5570129|FD-Score:-3.10|P-value:8.07E-4 ID:SGTC_2162|Compound:5663267|FD-Score:3.40|P-value:2.81E-4 ID:SGTC_2265|Compound:7929201|FD-Score:3.14|P-value:7.22E-4 ID:SGTC_2309|Compound:7664088|FD-Score:5.48|P-value:1.29E-8 ID:SGTC_2312|Compound:7706453|FD-Score:3.24|P-value:5.12E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.86|P-value:4.39E-5 ID:SGTC_2336|Compound:9001819|FD-Score:3.61|P-value:1.22E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:6.19|P-value:1.66E-10 ID:SGTC_2411|Compound:st077133|FD-Score:3.30|P-value:4.01E-4 ID:SGTC_2413|Compound:inermin|FD-Score:-4.14|P-value:1.24E-5 ID:SGTC_2415|Compound:st077333|FD-Score:6.12|P-value:2.61E-10 ID:SGTC_2418|Compound:pentamidine|FD-Score:-4.12|P-value:1.36E-5 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.49|P-value:2.02E-4 ID:SGTC_2459|Compound:5811157|FD-Score:-3.89|P-value:3.84E-5 ID:SGTC_2469|Compound:5655019|FD-Score:5.05|P-value:1.50E-7 ID:SGTC_2474|Compound:5321570|FD-Score:3.97|P-value:2.77E-5 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:3.12|P-value:7.73E-4 ID:SGTC_2523|Compound:spermine|FD-Score:3.10|P-value:8.20E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:-3.53|P-value:1.61E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:-3.49|P-value:1.87E-4 ID:SGTC_2764|Compound:methyldopa|FD-Score:7.77|P-value:1.52E-15 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.36|P-value:3.08E-4 ID:SGTC_2797|Compound:7859098|FD-Score:6.40|P-value:4.14E-11 ID:SGTC_2798|Compound:7813637|FD-Score:-3.28|P-value:4.15E-4 ID:SGTC_3215|Compound:9129942|FD-Score:3.63|P-value:1.16E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-6.90|P-value:1.15E-12 ID:SGTC_64|Compound:000l-0018|FD-Score:3.30|P-value:4.09E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:4.18|P-value:1.11E-5 ID:SGTC_174|Compound:3553-0389|FD-Score:-3.15|P-value:6.63E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:-4.69|P-value:9.11E-7 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.10|P-value:8.33E-4 ID:SGTC_235|Compound:0330-0087|FD-Score:3.88|P-value:4.13E-5 ID:SGTC_271|Compound:rotenone|FD-Score:3.13|P-value:7.41E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:3.53|P-value:1.73E-4 ID:SGTC_480|Compound:am 92016|FD-Score:4.12|P-value:1.46E-5 ID:SGTC_501|Compound:nsc-95397|FD-Score:-3.05|P-value:9.64E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:3.34|P-value:3.44E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:4.84|P-value:4.55E-7 ID:SGTC_538|Compound:2922-0838|FD-Score:-4.07|P-value:1.75E-5 ID:SGTC_542|Compound:3381-0244|FD-Score:3.95|P-value:2.98E-5 ID:SGTC_574|Compound:1315-0397|FD-Score:-3.21|P-value:5.46E-4 ID:SGTC_658|Compound:3253-0951|FD-Score:3.44|P-value:2.42E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:4.37|P-value:4.68E-6 ID:SGTC_685|Compound:1278-0033|FD-Score:5.88|P-value:1.17E-9 ID:SGTC_730|Compound:1436-0052|FD-Score:4.73|P-value:7.68E-7 ID:SGTC_758|Compound:0868-0029|FD-Score:-8.99|P-value:3.10E-20 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:3.24|P-value:5.09E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:3.33|P-value:3.64E-4 ID:SGTC_1195|Compound:1502-0147|FD-Score:5.45|P-value:1.54E-8 ID:SGTC_1199|Compound:1638-0010|FD-Score:-3.14|P-value:6.96E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.47|P-value:2.15E-4 ID:SGTC_1337|Compound:1486-1011|FD-Score:4.52|P-value:2.24E-6 ID:SGTC_1388|Compound:3346-4487|FD-Score:3.35|P-value:3.38E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:-3.25|P-value:4.62E-4 ID:SGTC_1419|Compound:3993-2473|FD-Score:-3.57|P-value:1.42E-4 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.56|P-value:1.50E-4 ID:SGTC_1497|Compound:4476-2978|FD-Score:3.19|P-value:6.07E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:3.83|P-value:4.99E-5 ID:SGTC_1655|Compound:st012546|FD-Score:4.18|P-value:1.10E-5 ID:SGTC_1674|Compound:st016616|FD-Score:3.35|P-value:3.39E-4 ID:SGTC_1688|Compound:st021091|FD-Score:3.46|P-value:2.26E-4 ID:SGTC_1716|Compound:st033144|FD-Score:6.46|P-value:2.61E-11 ID:SGTC_1818|Compound:st052136|FD-Score:3.87|P-value:4.33E-5 ID:SGTC_1888|Compound:azatryptophan|FD-Score:4.23|P-value:8.94E-6 ID:SGTC_2025|Compound:5147754|FD-Score:3.54|P-value:1.63E-4 ID:SGTC_2032|Compound:5185112|FD-Score:3.37|P-value:3.19E-4 ID:SGTC_2038|Compound:4100038|FD-Score:3.17|P-value:6.46E-4 ID:SGTC_2051|Compound:5263410|FD-Score:-3.77|P-value:6.11E-5 ID:SGTC_2075|Compound:5212009|FD-Score:3.48|P-value:2.09E-4 ID:SGTC_2154|Compound:5570129|FD-Score:-3.10|P-value:8.07E-4 ID:SGTC_2162|Compound:5663267|FD-Score:3.40|P-value:2.81E-4 ID:SGTC_2265|Compound:7929201|FD-Score:3.14|P-value:7.22E-4 ID:SGTC_2309|Compound:7664088|FD-Score:5.48|P-value:1.29E-8 ID:SGTC_2312|Compound:7706453|FD-Score:3.24|P-value:5.12E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.86|P-value:4.39E-5 ID:SGTC_2336|Compound:9001819|FD-Score:3.61|P-value:1.22E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:6.19|P-value:1.66E-10 ID:SGTC_2411|Compound:st077133|FD-Score:3.30|P-value:4.01E-4 ID:SGTC_2413|Compound:inermin|FD-Score:-4.14|P-value:1.24E-5 ID:SGTC_2415|Compound:st077333|FD-Score:6.12|P-value:2.61E-10 ID:SGTC_2418|Compound:pentamidine|FD-Score:-4.12|P-value:1.36E-5 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.49|P-value:2.02E-4 ID:SGTC_2459|Compound:5811157|FD-Score:-3.89|P-value:3.84E-5 ID:SGTC_2469|Compound:5655019|FD-Score:5.05|P-value:1.50E-7 ID:SGTC_2474|Compound:5321570|FD-Score:3.97|P-value:2.77E-5 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:3.12|P-value:7.73E-4 ID:SGTC_2523|Compound:spermine|FD-Score:3.10|P-value:8.20E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:-3.53|P-value:1.61E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:-3.49|P-value:1.87E-4 ID:SGTC_2764|Compound:methyldopa|FD-Score:7.77|P-value:1.52E-15 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.36|P-value:3.08E-4 ID:SGTC_2797|Compound:7859098|FD-Score:6.40|P-value:4.14E-11 ID:SGTC_2798|Compound:7813637|FD-Score:-3.28|P-value:4.15E-4 ID:SGTC_3215|Compound:9129942|FD-Score:3.63|P-value:1.16E-4

Top fitness defect scores for YKL034W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2764 methyldopa 7.77 94.7 µM 1.52E-15 3 4.03E-15
2 SGTC_1716 st033144 6.46 30.6 µM 2.61E-11 3 5.12E-11
3 SGTC_2797 7859098 6.40 81.8 µM 4.14E-11 5 7.99E-11
4 SGTC_2405 ticlatone 6.19 3.5 µM 1.66E-10 29 3.06E-10
5 SGTC_2415 st077333 6.12 57.7 µM 2.61E-10 12 4.75E-10
6 SGTC_685 1278-0033 5.88 54.9 µM 1.17E-9 60S ribosome export 3 2.03E-9
7 SGTC_2309 7664088 5.48 163.9 µM 1.29E-8 7 2.09E-8
8 SGTC_1195 1502-0147 5.45 41.1 µM 1.54E-8 6 2.48E-8
9 SGTC_2469 5655019 5.05 90.8 µM 1.50E-7 9 2.26E-7
10 SGTC_536 3852-0578 4.84 10.6 µM 4.55E-7 12 6.62E-7
11 SGTC_730 1436-0052 4.73 41.2 µM 7.68E-7 mitochondrial processes 5 1.10E-6
12 SGTC_1337 1486-1011 4.52 40.3 µM 2.24E-6 9 3.10E-6
13 SGTC_670 4204-0025 4.37 119.0 µM 4.68E-6 copper-dependent oxidative stress 24 6.35E-6
14 SGTC_1888 azatryptophan 4.23 97.5 µM 8.94E-6 11 1.19E-5
15 SGTC_1655 st012546 4.18 55.1 µM 1.10E-5 25 1.46E-5
16 SGTC_67 0335-0847 4.18 266.0 µM 1.11E-5 ubiquinone biosynthesis & proteasome 105 1.46E-5
17 SGTC_480 am 92016 4.12 50.0 µM 1.46E-5 11 1.91E-5
18 SGTC_2474 5321570 3.97 43.3 µM 2.77E-5 12 3.57E-5
19 SGTC_542 3381-0244 3.95 89.3 µM 2.98E-5 39 3.83E-5
20 SGTC_235 0330-0087 3.88 5.0 mM 4.13E-5 TSC3-RPN4 25 5.26E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.201 8.39E-32 YHL045W_d YHL045W_d hom Putative protein of unknown function; not an essential gene
0.194 1.04E-29 YGL090W LIF1 hom Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein
0.193 2.13E-29 YDL155W CLB3 hom B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress
0.183 1.45E-26 YML029W USA1 hom Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p
0.178 2.17E-25 YLR317W_d YLR317W_d het Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
0.173 6.17E-24 YPL246C RBD2 hom Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p
0.169 8.45E-23 YNL123W NMA111 hom Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
0.164 1.12E-21 YOR271C_p FSF1_p hom Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis
0.153 5.13E-19 YLR040C_p YLR040C_p hom Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential
0.150 2.37E-18 YOR282W_d YOR282W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.143 8.83E-17 YLR034C SMF3 hom Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress
0.143 9.30E-17 YLR440C SEC39 het Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope
0.140 4.25E-16 YGL114W_p YGL114W_p hom Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters
0.140 4.39E-16 YIL009W FAA3 hom Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.138 1.03E-15 YDL022W GPD1 hom NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity