YKL045W / PRI2

Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL045W / PRI2

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:3.56|P-value:1.98E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:3.55|P-value:2.05E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:3.57|P-value:1.93E-4 ID:SGTC_194|Compound:1486-1323|FD-Score:3.23|P-value:6.46E-4 ID:SGTC_290|Compound:2043-6722|FD-Score:3.30|P-value:5.06E-4 ID:SGTC_559|Compound:1550-0661|FD-Score:3.55|P-value:2.05E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:-3.85|P-value:6.08E-5 ID:SGTC_632|Compound:0781-2353|FD-Score:-3.75|P-value:8.93E-5 ID:SGTC_679|Compound:0422-0091|FD-Score:4.98|P-value:3.51E-7 ID:SGTC_704|Compound:1310-0270|FD-Score:4.38|P-value:6.39E-6 ID:SGTC_758|Compound:0868-0029|FD-Score:-3.55|P-value:1.94E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:3.74|P-value:9.69E-5 ID:SGTC_847|Compound:0342-0274|FD-Score:-3.46|P-value:2.70E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:-3.26|P-value:5.66E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:3.28|P-value:5.42E-4 ID:SGTC_1141|Compound:3253-0389|FD-Score:-3.36|P-value:3.98E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:3.14|P-value:8.94E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:-3.17|P-value:7.70E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.43|P-value:3.16E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:-3.18|P-value:7.29E-4 ID:SGTC_1886|Compound:st057356|FD-Score:3.31|P-value:4.86E-4 ID:SGTC_1969|Compound:st069479|FD-Score:-3.21|P-value:6.56E-4 ID:SGTC_2092|Compound:5303694|FD-Score:3.74|P-value:9.74E-5 ID:SGTC_2099|Compound:5377090|FD-Score:-3.24|P-value:5.99E-4 ID:SGTC_2104|Compound:5389443|FD-Score:3.52|P-value:2.25E-4 ID:SGTC_2143|Compound:5623677|FD-Score:-3.81|P-value:7.05E-5 ID:SGTC_2181|Compound:6316240|FD-Score:4.29|P-value:9.58E-6 ID:SGTC_2360|Compound:9040895|FD-Score:3.15|P-value:8.72E-4 ID:SGTC_2428|Compound:5615643|FD-Score:3.35|P-value:4.23E-4 ID:SGTC_2434|Compound:5670830|FD-Score:4.17|P-value:1.69E-5 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:-3.48|P-value:2.50E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:4.90|P-value:5.22E-7 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.69|P-value:1.18E-4 ID:SGTC_2713|Compound:st079149|FD-Score:3.52|P-value:2.31E-4 ID:SGTC_2716|Compound:st080858|FD-Score:3.15|P-value:8.49E-4 ID:SGTC_2770|Compound:toltrazuril|FD-Score:-3.22|P-value:6.37E-4 ID:SGTC_2876|Compound:9059903|FD-Score:-4.30|P-value:8.80E-6 ID:SGTC_2904|Compound:7225135|FD-Score:3.53|P-value:2.25E-4 ID:SGTC_2977|Compound:9011197|FD-Score:-3.39|P-value:3.52E-4 ID:SGTC_3011|Compound:9081274|FD-Score:3.45|P-value:3.00E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:3.56|P-value:1.98E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:3.55|P-value:2.05E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:3.57|P-value:1.93E-4 ID:SGTC_194|Compound:1486-1323|FD-Score:3.23|P-value:6.46E-4 ID:SGTC_290|Compound:2043-6722|FD-Score:3.30|P-value:5.06E-4 ID:SGTC_559|Compound:1550-0661|FD-Score:3.55|P-value:2.05E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:-3.85|P-value:6.08E-5 ID:SGTC_632|Compound:0781-2353|FD-Score:-3.75|P-value:8.93E-5 ID:SGTC_679|Compound:0422-0091|FD-Score:4.98|P-value:3.51E-7 ID:SGTC_704|Compound:1310-0270|FD-Score:4.38|P-value:6.39E-6 ID:SGTC_758|Compound:0868-0029|FD-Score:-3.55|P-value:1.94E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:3.74|P-value:9.69E-5 ID:SGTC_847|Compound:0342-0274|FD-Score:-3.46|P-value:2.70E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:-3.26|P-value:5.66E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:3.28|P-value:5.42E-4 ID:SGTC_1141|Compound:3253-0389|FD-Score:-3.36|P-value:3.98E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:3.14|P-value:8.94E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:-3.17|P-value:7.70E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.43|P-value:3.16E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:-3.18|P-value:7.29E-4 ID:SGTC_1886|Compound:st057356|FD-Score:3.31|P-value:4.86E-4 ID:SGTC_1969|Compound:st069479|FD-Score:-3.21|P-value:6.56E-4 ID:SGTC_2092|Compound:5303694|FD-Score:3.74|P-value:9.74E-5 ID:SGTC_2099|Compound:5377090|FD-Score:-3.24|P-value:5.99E-4 ID:SGTC_2104|Compound:5389443|FD-Score:3.52|P-value:2.25E-4 ID:SGTC_2143|Compound:5623677|FD-Score:-3.81|P-value:7.05E-5 ID:SGTC_2181|Compound:6316240|FD-Score:4.29|P-value:9.58E-6 ID:SGTC_2360|Compound:9040895|FD-Score:3.15|P-value:8.72E-4 ID:SGTC_2428|Compound:5615643|FD-Score:3.35|P-value:4.23E-4 ID:SGTC_2434|Compound:5670830|FD-Score:4.17|P-value:1.69E-5 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:-3.48|P-value:2.50E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:4.90|P-value:5.22E-7 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.69|P-value:1.18E-4 ID:SGTC_2713|Compound:st079149|FD-Score:3.52|P-value:2.31E-4 ID:SGTC_2716|Compound:st080858|FD-Score:3.15|P-value:8.49E-4 ID:SGTC_2770|Compound:toltrazuril|FD-Score:-3.22|P-value:6.37E-4 ID:SGTC_2876|Compound:9059903|FD-Score:-4.30|P-value:8.80E-6 ID:SGTC_2904|Compound:7225135|FD-Score:3.53|P-value:2.25E-4 ID:SGTC_2977|Compound:9011197|FD-Score:-3.39|P-value:3.52E-4 ID:SGTC_3011|Compound:9081274|FD-Score:3.45|P-value:3.00E-4

Top fitness defect scores for YKL045W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_679 0422-0091 4.98 41.4 µM 3.51E-7 Golgi 5 3.16E-7 0.28
2 SGTC_2632 3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone 4.90 100.0 µM 5.22E-7 2 4.71E-7 1.08
3 SGTC_704 1310-0270 4.38 142.0 µM 6.39E-6 2 5.86E-6 0.20
4 SGTC_2181 6316240 4.29 54.3 µM 9.58E-6 2 8.81E-6 0.83
5 SGTC_2434 5670830 4.17 169.9 µM 1.69E-5 4 1.56E-5 0.04
6 SGTC_839 0122-0028 3.74 230.0 µM 9.69E-5 RSC complex & mRNA processing 41 9.05E-5 0.17
7 SGTC_2092 5303694 3.74 80.7 µM 9.74E-5 TSC3-RPN4 11 9.09E-5 0.18
8 SGTC_2661 dactinomycin 3.69 13.0 µM 1.18E-4 redox potentiating 2 1.10E-4 0.02
9 SGTC_138 0850-0259 3.57 40.9 µM 1.93E-4 cell wall 57 1.81E-4 0.01
10 SGTC_43 0570-0105 3.56 72.5 µM 1.98E-4 ERG2 13 1.86E-4 0.13
11 SGTC_44 0929-0055 3.55 61.2 µM 2.05E-4 11 1.92E-4 0.02
12 SGTC_559 1550-0661 3.55 158.0 µM 2.05E-4 2 1.92E-4 0.34
13 SGTC_2904 7225135 3.53 42.0 µM 2.25E-4 2 2.11E-4 0.09
14 SGTC_2104 5389443 3.52 200.0 µM 2.25E-4 10 2.12E-4 0.16
15 SGTC_2713 st079149 3.52 53.4 µM 2.31E-4 8 2.17E-4 0.09
16 SGTC_3011 9081274 3.45 71.4 µM 3.00E-4 6 2.82E-4 0.03
17 SGTC_1446 4239-0190 3.43 62.0 µM 3.16E-4 10 2.97E-4 0.07
18 SGTC_2428 5615643 3.35 7.8 µM 4.23E-4 12 3.99E-4 0.06
19 SGTC_1886 st057356 3.31 49.9 µM 4.86E-4 60S ribosome export 13 4.59E-4 0.06
20 SGTC_290 2043-6722 3.30 48.1 µM 5.06E-4 5 4.78E-4 0.26

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.095 3.33E-8 YJL080C SCP160 hom Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
0.086 7.06E-7 YPL269W KAR9 hom Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
0.085 7.82E-7 YGR085C RPL11B hom Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication
0.084 1.02E-6 YOR051C ETT1 hom Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
0.082 1.71E-6 YPL198W RPL7B hom Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication
0.081 2.66E-6 YMR294W JNM1 hom Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B
0.079 4.50E-6 YML025C YML6 het Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
0.078 5.77E-6 YPL009C TAE2 hom Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.077 7.48E-6 YHR129C ARP1 hom Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin
0.075 1.25E-5 YIR035C_p YIR035C_p hom Putative cytoplasmic short-chain dehydrogenase/reductase
0.075 1.37E-5 YER156C_p YER156C_p hom Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1
0.074 1.84E-5 YPL213W LEA1 hom Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein
0.073 2.04E-5 YKR100C SKG1 hom Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell
0.073 2.10E-5 YDR416W SYF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans
0.073 2.38E-5 YNL176C_p TDA7_p hom Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele