YKL049C / CSE4

Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL049C / CSE4

Click on Significant Values for Screen Details ID:SGTC_81|Compound:0411-0046|FD-Score:-3.64|P-value:6.33E-6 ID:SGTC_82|Compound:0054-0107|FD-Score:-3.10|P-value:1.03E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:-2.72|P-value:5.61E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:4.71|P-value:8.44E-9 ID:SGTC_119|Compound:0121-0043|FD-Score:3.96|P-value:1.03E-6 ID:SGTC_319|Compound:1348-1596|FD-Score:6.13|P-value:1.05E-13 ID:SGTC_366|Compound:0181-0206|FD-Score:4.22|P-value:2.13E-7 ID:SGTC_367|Compound:0335-0881|FD-Score:2.98|P-value:1.82E-4 ID:SGTC_395|Compound:anisomycin|FD-Score:2.84|P-value:3.34E-4 ID:SGTC_396|Compound:dithiazanine|FD-Score:3.11|P-value:9.63E-5 ID:SGTC_535|Compound:2293-4129|FD-Score:2.94|P-value:2.12E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:-3.39|P-value:2.44E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:-2.98|P-value:1.77E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:2.89|P-value:2.71E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:2.71|P-value:5.78E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:3.01|P-value:1.56E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:5.61|P-value:9.33E-12 ID:SGTC_745|Compound:0469-0705|FD-Score:4.86|P-value:2.86E-9 ID:SGTC_752|Compound:1896-0090|FD-Score:-2.85|P-value:3.11E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:2.62|P-value:8.40E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:2.81|P-value:3.85E-4 ID:SGTC_1010|Compound:4112-3296|FD-Score:2.89|P-value:2.72E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:3.64|P-value:6.62E-6 ID:SGTC_1085|Compound:doxorubicin|FD-Score:3.65|P-value:6.04E-6 ID:SGTC_1086|Compound:cerivastatin|FD-Score:3.49|P-value:1.47E-5 ID:SGTC_1169|Compound:aniline yellow|FD-Score:2.78|P-value:4.32E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:2.77|P-value:4.62E-4 ID:SGTC_1364|Compound:1653-0028|FD-Score:-2.88|P-value:2.84E-4 ID:SGTC_1413|Compound:3910-0535|FD-Score:3.40|P-value:2.30E-5 ID:SGTC_1418|Compound:3966-3099|FD-Score:3.00|P-value:1.66E-4 ID:SGTC_1428|Compound:4049-0204|FD-Score:3.57|P-value:9.60E-6 ID:SGTC_1490|Compound:4089-0339|FD-Score:2.68|P-value:6.62E-4 ID:SGTC_1558|Compound:st069300|FD-Score:-2.61|P-value:8.65E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:4.13|P-value:3.91E-7 ID:SGTC_1729|Compound:st035615|FD-Score:2.92|P-value:2.41E-4 ID:SGTC_1733|Compound:st037357|FD-Score:3.71|P-value:4.47E-6 ID:SGTC_1771|Compound:st048882|FD-Score:-3.05|P-value:1.26E-4 ID:SGTC_1773|Compound:st048542|FD-Score:2.80|P-value:3.95E-4 ID:SGTC_1830|Compound:st055982|FD-Score:2.80|P-value:3.99E-4 ID:SGTC_1850|Compound:st056238|FD-Score:-2.60|P-value:9.27E-4 ID:SGTC_1851|Compound:st056236|FD-Score:3.46|P-value:1.68E-5 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:2.83|P-value:3.44E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:2.70|P-value:6.13E-4 ID:SGTC_2007|Compound:4011539|FD-Score:-2.75|P-value:4.93E-4 ID:SGTC_2272|Compound:7805186|FD-Score:-3.34|P-value:3.14E-5 ID:SGTC_2275|Compound:7947463|FD-Score:2.81|P-value:3.80E-4 ID:SGTC_2311|Compound:7694451|FD-Score:4.02|P-value:7.27E-7 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-3.36|P-value:2.77E-5 ID:SGTC_2805|Compound:7950783|FD-Score:-2.98|P-value:1.74E-4 ID:SGTC_2970|Compound:9090938|FD-Score:2.92|P-value:2.40E-4 ID:SGTC_3000|Compound:9075062|FD-Score:2.78|P-value:4.39E-4 ID:SGTC_3289|Compound:9119912|FD-Score:-2.87|P-value:2.93E-4 ID:SGTC_3300|Compound:9124615|FD-Score:-2.86|P-value:3.00E-4 ID:SGTC_3324|Compound:9138759|FD-Score:-2.64|P-value:7.80E-4 ID:SGTC_3326|Compound:9140064|FD-Score:-4.12|P-value:3.96E-7 ID:SGTC_3329|Compound:9141233|FD-Score:2.60|P-value:9.45E-4 ID:SGTC_3341|Compound:9147222|FD-Score:-3.32|P-value:3.46E-5 ID:SGTC_81|Compound:0411-0046|FD-Score:-3.64|P-value:6.33E-6 ID:SGTC_82|Compound:0054-0107|FD-Score:-3.10|P-value:1.03E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:-2.72|P-value:5.61E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:4.71|P-value:8.44E-9 ID:SGTC_119|Compound:0121-0043|FD-Score:3.96|P-value:1.03E-6 ID:SGTC_319|Compound:1348-1596|FD-Score:6.13|P-value:1.05E-13 ID:SGTC_366|Compound:0181-0206|FD-Score:4.22|P-value:2.13E-7 ID:SGTC_367|Compound:0335-0881|FD-Score:2.98|P-value:1.82E-4 ID:SGTC_395|Compound:anisomycin|FD-Score:2.84|P-value:3.34E-4 ID:SGTC_396|Compound:dithiazanine|FD-Score:3.11|P-value:9.63E-5 ID:SGTC_535|Compound:2293-4129|FD-Score:2.94|P-value:2.12E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:-3.39|P-value:2.44E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:-2.98|P-value:1.77E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:2.89|P-value:2.71E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:2.71|P-value:5.78E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:3.01|P-value:1.56E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:5.61|P-value:9.33E-12 ID:SGTC_745|Compound:0469-0705|FD-Score:4.86|P-value:2.86E-9 ID:SGTC_752|Compound:1896-0090|FD-Score:-2.85|P-value:3.11E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:2.62|P-value:8.40E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:2.81|P-value:3.85E-4 ID:SGTC_1010|Compound:4112-3296|FD-Score:2.89|P-value:2.72E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:3.64|P-value:6.62E-6 ID:SGTC_1085|Compound:doxorubicin|FD-Score:3.65|P-value:6.04E-6 ID:SGTC_1086|Compound:cerivastatin|FD-Score:3.49|P-value:1.47E-5 ID:SGTC_1169|Compound:aniline yellow|FD-Score:2.78|P-value:4.32E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:2.77|P-value:4.62E-4 ID:SGTC_1364|Compound:1653-0028|FD-Score:-2.88|P-value:2.84E-4 ID:SGTC_1413|Compound:3910-0535|FD-Score:3.40|P-value:2.30E-5 ID:SGTC_1418|Compound:3966-3099|FD-Score:3.00|P-value:1.66E-4 ID:SGTC_1428|Compound:4049-0204|FD-Score:3.57|P-value:9.60E-6 ID:SGTC_1490|Compound:4089-0339|FD-Score:2.68|P-value:6.62E-4 ID:SGTC_1558|Compound:st069300|FD-Score:-2.61|P-value:8.65E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:4.13|P-value:3.91E-7 ID:SGTC_1729|Compound:st035615|FD-Score:2.92|P-value:2.41E-4 ID:SGTC_1733|Compound:st037357|FD-Score:3.71|P-value:4.47E-6 ID:SGTC_1771|Compound:st048882|FD-Score:-3.05|P-value:1.26E-4 ID:SGTC_1773|Compound:st048542|FD-Score:2.80|P-value:3.95E-4 ID:SGTC_1830|Compound:st055982|FD-Score:2.80|P-value:3.99E-4 ID:SGTC_1850|Compound:st056238|FD-Score:-2.60|P-value:9.27E-4 ID:SGTC_1851|Compound:st056236|FD-Score:3.46|P-value:1.68E-5 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:2.83|P-value:3.44E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:2.70|P-value:6.13E-4 ID:SGTC_2007|Compound:4011539|FD-Score:-2.75|P-value:4.93E-4 ID:SGTC_2272|Compound:7805186|FD-Score:-3.34|P-value:3.14E-5 ID:SGTC_2275|Compound:7947463|FD-Score:2.81|P-value:3.80E-4 ID:SGTC_2311|Compound:7694451|FD-Score:4.02|P-value:7.27E-7 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-3.36|P-value:2.77E-5 ID:SGTC_2805|Compound:7950783|FD-Score:-2.98|P-value:1.74E-4 ID:SGTC_2970|Compound:9090938|FD-Score:2.92|P-value:2.40E-4 ID:SGTC_3000|Compound:9075062|FD-Score:2.78|P-value:4.39E-4 ID:SGTC_3289|Compound:9119912|FD-Score:-2.87|P-value:2.93E-4 ID:SGTC_3300|Compound:9124615|FD-Score:-2.86|P-value:3.00E-4 ID:SGTC_3324|Compound:9138759|FD-Score:-2.64|P-value:7.80E-4 ID:SGTC_3326|Compound:9140064|FD-Score:-4.12|P-value:3.96E-7 ID:SGTC_3329|Compound:9141233|FD-Score:2.60|P-value:9.45E-4 ID:SGTC_3341|Compound:9147222|FD-Score:-3.32|P-value:3.46E-5

Top fitness defect scores for YKL049C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_319 1348-1596 6.13 45.5 µM 1.05E-13 RSC & ERG11 3 4.39E-10 1.04
2 SGTC_740 k014-0057 5.61 261.0 µM 9.33E-12 1 1.02E-8 2.07
3 SGTC_745 0469-0705 4.86 296.0 µM 2.86E-9 mitochondrial processes 9 5.77E-7 0.24
4 SGTC_112 phenothiazine 4.71 287.2 µM 8.44E-9 unfolded protein response 2 1.24E-6 0.16
5 SGTC_366 0181-0206 4.22 4.3 µM 2.13E-7 Golgi 8 1.21E-5 0.15
6 SGTC_1576 erythromycin ethylsuccinate 4.13 23.2 µM 3.91E-7 5 1.85E-5 0.42
7 SGTC_2311 7694451 4.02 200.0 µM 7.27E-7 8 2.87E-5 0.09
8 SGTC_119 0121-0043 3.96 24.8 µM 1.03E-6 mitochondrial stress 22 3.69E-5 0.07
9 SGTC_1733 st037357 3.71 54.9 µM 4.47E-6 60S ribosome export 10 1.04E-4 0.09
10 SGTC_1085 doxorubicin 3.65 7.7 µM 6.04E-6 anthracycline transcription coupled DNA repair 10 1.29E-4 0.15
11 SGTC_1060 3448-7350 3.64 82.0 µM 6.62E-6 Golgi 15 1.37E-4 0.11
12 SGTC_1428 4049-0204 3.57 61.7 µM 9.60E-6 2 1.79E-4 0.05
13 SGTC_1086 cerivastatin 3.49 2.2 µM 1.47E-5 azole & statin 6 2.43E-4 0.28
14 SGTC_1851 st056236 3.46 18.9 µM 1.68E-5 8 2.66E-4 0.01
15 SGTC_1413 3910-0535 3.40 34.4 µM 2.30E-5 6 3.33E-4 0.06
16 SGTC_396 dithiazanine 3.11 1.2 µM 9.63E-5 8 9.23E-4 0.09
17 SGTC_704 1310-0270 3.01 142.0 µM 1.56E-4 17 0.00130 0.05
18 SGTC_1418 3966-3099 3.00 2.4 µM 1.66E-4 16 0.00136 0.02
19 SGTC_367 0335-0881 2.98 7.1 µM 1.82E-4 ubiquinone biosynthesis & proteasome 41 0.00145 3.09E-4
20 SGTC_535 2293-4129 2.94 48.2 µM 2.12E-4 NEO1 23 0.00162 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.108 2.95E-10 YMR117C SPC24 het Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
0.091 1.32E-7 YOR326W MYO2 het Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication
0.086 5.76E-7 YOR127W RGA1 hom GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.081 2.73E-6 YOR263C_d YOR263C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W
0.081 2.86E-6 YPL178W CBC2 hom Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif
0.080 3.83E-6 YJL002C OST1 het Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
0.077 8.66E-6 YNL277W-A_p YNL277W-A_p hom Putative protein of unknown function
0.074 1.68E-5 YGL021W ALK1 hom Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins
0.074 1.88E-5 YDR167W TAF10 het Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
0.071 3.80E-5 YKL078W DHR2 het Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
0.071 4.26E-5 YLR390W-A CCW14 hom Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall
0.070 5.24E-5 YIL150C MCM10 het Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
0.070 5.39E-5 YOL052C-A DDR2 hom Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication
0.069 6.35E-5 YLR361C-A_p YLR361C-A_p hom Putative protein of unknown function
0.069 6.52E-5 YGR242W_d YGR242W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C