YKL052C / ASK1

Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL052C / ASK1

Click on Significant Values for Screen Details ID:SGTC_75|Compound:0159-0059|FD-Score:2.94|P-value:1.55E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:2.91|P-value:1.79E-4 ID:SGTC_333|Compound:3486-0131|FD-Score:3.74|P-value:2.29E-6 ID:SGTC_339|Compound:3091-4833|FD-Score:6.19|P-value:1.52E-14 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.05|P-value:8.97E-5 ID:SGTC_380|Compound:3970-0968|FD-Score:-2.64|P-value:6.08E-4 ID:SGTC_384|Compound:k007-0144|FD-Score:3.12|P-value:6.55E-5 ID:SGTC_401|Compound:neomycin|FD-Score:-2.69|P-value:4.89E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:-2.91|P-value:1.79E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:2.76|P-value:3.55E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:2.69|P-value:4.89E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:2.59|P-value:7.36E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.15|P-value:1.80E-7 ID:SGTC_1198|Compound:1612-1005|FD-Score:2.88|P-value:2.11E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:2.79|P-value:3.14E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:2.73|P-value:4.01E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:-3.07|P-value:8.35E-5 ID:SGTC_1399|Compound:3562-5772|FD-Score:16.70|P-value:3.41E-93 ID:SGTC_1457|Compound:k015-0032|FD-Score:-4.52|P-value:1.45E-8 ID:SGTC_1647|Compound:st011722|FD-Score:-2.61|P-value:6.71E-4 ID:SGTC_1682|Compound:st012968|FD-Score:3.56|P-value:6.26E-6 ID:SGTC_1683|Compound:st010134|FD-Score:-3.38|P-value:1.69E-5 ID:SGTC_1691|Compound:st019933|FD-Score:3.00|P-value:1.18E-4 ID:SGTC_1726|Compound:st035556|FD-Score:2.80|P-value:2.97E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-2.55|P-value:8.70E-4 ID:SGTC_1766|Compound:st045414|FD-Score:10.70|P-value:5.54E-40 ID:SGTC_1915|Compound:st067105|FD-Score:-3.59|P-value:5.35E-6 ID:SGTC_1930|Compound:st074634|FD-Score:2.68|P-value:5.10E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.00|P-value:1.19E-4 ID:SGTC_1959|Compound:st077091|FD-Score:18.90|P-value:1.50E-119 ID:SGTC_2057|Compound:5236571|FD-Score:10.90|P-value:6.19E-41 ID:SGTC_2103|Compound:5378138|FD-Score:3.11|P-value:6.81E-5 ID:SGTC_2143|Compound:5623677|FD-Score:2.94|P-value:1.59E-4 ID:SGTC_2156|Compound:5649887|FD-Score:29.70|P-value:8.21E-291 ID:SGTC_2247|Compound:cerulenin|FD-Score:-3.98|P-value:5.27E-7 ID:SGTC_2507|Compound:purpurin|FD-Score:5.29|P-value:4.50E-11 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:8.03|P-value:3.60E-23 ID:SGTC_2686|Compound:alizarin|FD-Score:3.56|P-value:6.19E-6 ID:SGTC_2791|Compound:5663188|FD-Score:12.50|P-value:2.71E-53 ID:SGTC_2794|Compound:7785382|FD-Score:4.01|P-value:4.38E-7 ID:SGTC_2801|Compound:7907800|FD-Score:-3.22|P-value:3.89E-5 ID:SGTC_3125|Compound:9127534|FD-Score:2.99|P-value:1.23E-4 ID:SGTC_3133|Compound:9127736|FD-Score:2.92|P-value:1.68E-4 ID:SGTC_3214|Compound:9129694|FD-Score:2.90|P-value:1.90E-4 ID:SGTC_3220|Compound:9131269|FD-Score:-3.70|P-value:2.89E-6 ID:SGTC_75|Compound:0159-0059|FD-Score:2.94|P-value:1.55E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:2.91|P-value:1.79E-4 ID:SGTC_333|Compound:3486-0131|FD-Score:3.74|P-value:2.29E-6 ID:SGTC_339|Compound:3091-4833|FD-Score:6.19|P-value:1.52E-14 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.05|P-value:8.97E-5 ID:SGTC_380|Compound:3970-0968|FD-Score:-2.64|P-value:6.08E-4 ID:SGTC_384|Compound:k007-0144|FD-Score:3.12|P-value:6.55E-5 ID:SGTC_401|Compound:neomycin|FD-Score:-2.69|P-value:4.89E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:-2.91|P-value:1.79E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:2.76|P-value:3.55E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:2.69|P-value:4.89E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:2.59|P-value:7.36E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.15|P-value:1.80E-7 ID:SGTC_1198|Compound:1612-1005|FD-Score:2.88|P-value:2.11E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:2.79|P-value:3.14E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:2.73|P-value:4.01E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:-3.07|P-value:8.35E-5 ID:SGTC_1399|Compound:3562-5772|FD-Score:16.70|P-value:3.41E-93 ID:SGTC_1457|Compound:k015-0032|FD-Score:-4.52|P-value:1.45E-8 ID:SGTC_1647|Compound:st011722|FD-Score:-2.61|P-value:6.71E-4 ID:SGTC_1682|Compound:st012968|FD-Score:3.56|P-value:6.26E-6 ID:SGTC_1683|Compound:st010134|FD-Score:-3.38|P-value:1.69E-5 ID:SGTC_1691|Compound:st019933|FD-Score:3.00|P-value:1.18E-4 ID:SGTC_1726|Compound:st035556|FD-Score:2.80|P-value:2.97E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-2.55|P-value:8.70E-4 ID:SGTC_1766|Compound:st045414|FD-Score:10.70|P-value:5.54E-40 ID:SGTC_1915|Compound:st067105|FD-Score:-3.59|P-value:5.35E-6 ID:SGTC_1930|Compound:st074634|FD-Score:2.68|P-value:5.10E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.00|P-value:1.19E-4 ID:SGTC_1959|Compound:st077091|FD-Score:18.90|P-value:1.50E-119 ID:SGTC_2057|Compound:5236571|FD-Score:10.90|P-value:6.19E-41 ID:SGTC_2103|Compound:5378138|FD-Score:3.11|P-value:6.81E-5 ID:SGTC_2143|Compound:5623677|FD-Score:2.94|P-value:1.59E-4 ID:SGTC_2156|Compound:5649887|FD-Score:29.70|P-value:8.21E-291 ID:SGTC_2247|Compound:cerulenin|FD-Score:-3.98|P-value:5.27E-7 ID:SGTC_2507|Compound:purpurin|FD-Score:5.29|P-value:4.50E-11 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:8.03|P-value:3.60E-23 ID:SGTC_2686|Compound:alizarin|FD-Score:3.56|P-value:6.19E-6 ID:SGTC_2791|Compound:5663188|FD-Score:12.50|P-value:2.71E-53 ID:SGTC_2794|Compound:7785382|FD-Score:4.01|P-value:4.38E-7 ID:SGTC_2801|Compound:7907800|FD-Score:-3.22|P-value:3.89E-5 ID:SGTC_3125|Compound:9127534|FD-Score:2.99|P-value:1.23E-4 ID:SGTC_3133|Compound:9127736|FD-Score:2.92|P-value:1.68E-4 ID:SGTC_3214|Compound:9129694|FD-Score:2.90|P-value:1.90E-4 ID:SGTC_3220|Compound:9131269|FD-Score:-3.70|P-value:2.89E-6

Top fitness defect scores for YKL052C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2156 5649887 29.70 115.0 µM 8.21E-291 RPP1 & pyrimidine depletion 1 4.45E-194 11.50
2 SGTC_1959 st077091 18.90 55.8 µM 1.50E-119 RPP1 & pyrimidine depletion 1 3.36E-80 4.63
3 SGTC_1399 3562-5772 16.70 25.0 µM 3.41E-93 RPP1 & pyrimidine depletion 1 1.15E-62 4.09
4 SGTC_2791 5663188 12.50 71.4 µM 2.71E-53 RPP1 & pyrimidine depletion 1 4.15E-36 3.53
5 SGTC_2057 5236571 10.90 38.3 µM 6.19E-41 RPP1 & pyrimidine depletion 5 7.10E-28 1.98
6 SGTC_1766 st045414 10.70 44.8 µM 5.54E-40 RPP1 & pyrimidine depletion 1 3.06E-27 1.51
7 SGTC_2509 methyl 7-deshydroxypyrogallin-4-carboxylate 8.03 54.6 µM 3.60E-23 RPP1 & pyrimidine depletion 4 5.02E-16 1.84
8 SGTC_339 3091-4833 6.19 71.4 µM 1.52E-14 iron homeostasis 1 2.95E-10 0.56
9 SGTC_2507 purpurin 5.29 79.7 µM 4.50E-11 copper-dependent oxidative stress 6 6.27E-8 0.16
10 SGTC_2794 7785382 4.01 27.3 µM 4.38E-7 13 3.04E-5 0.25
11 SGTC_333 3486-0131 3.74 40.1 µM 2.29E-6 amide catabolism 3 9.31E-5 0.15
12 SGTC_2686 alizarin 3.56 95.4 µM 6.19E-6 RPP1 & pyrimidine depletion 11 1.83E-4 0.24
13 SGTC_1682 st012968 3.56 530.0 nM 6.26E-6 8 1.84E-4 0.17
14 SGTC_384 k007-0144 3.12 5.2 µM 6.55E-5 ERAD & cell cycle 27 9.08E-4 0.03
15 SGTC_2103 5378138 3.11 10.0 µM 6.81E-5 9 9.33E-4 0.03
16 SGTC_1691 st019933 3.00 84.6 µM 1.18E-4 7 0.00136 0.05
17 SGTC_1935 st074532 3.00 41.6 µM 1.19E-4 20 0.00137 0.04
18 SGTC_3125 9127534 2.99 49.5 µM 1.23E-4 10 0.00140 0.01
19 SGTC_75 0159-0059 2.94 199.0 µM 1.55E-4 fatty acid desaturase (OLE1) 24 0.00163 0.02
20 SGTC_2143 5623677 2.94 140.5 µM 1.59E-4 6 0.00166 0.07

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.462 2.18E-177 YBR257W POP4 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.338 3.86E-90 YNL236W SIN4 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
0.261 1.91E-53 YKL053W_d YKL053W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1
0.186 2.59E-27 YHR062C RPP1 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.181 3.54E-26 YER133W GLC7 het Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
0.180 7.90E-26 YJR007W SUI2 het Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
0.169 7.47E-23 YKR038C KAE1 het Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription
0.158 3.34E-20 YGL151W NUT1 hom Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription
0.152 8.06E-19 YKR083C DAD2 het Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
0.141 2.88E-16 YER025W GCD11 het Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met
0.139 5.97E-16 YNL235C_d YNL235C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex
0.127 1.85E-13 YFL013C IES1 hom Subunit of the INO80 chromatin remodeling complex
0.123 8.06E-13 YDR328C SKP1 het Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
0.120 2.62E-12 YOR341W RPA190 het RNA polymerase I largest subunit A190
0.119 3.95E-12 YPL238C_d YPL238C_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W