YKL053C-A / MDM35

Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL053C-A / MDM35

Click on Significant Values for Screen Details ID:SGTC_45|Compound:0kpi-0036|FD-Score:3.39|P-value:5.51E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:4.09|P-value:3.99E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:3.41|P-value:5.08E-4 ID:SGTC_274|Compound:sirtinol|FD-Score:-3.31|P-value:6.22E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:-3.51|P-value:3.09E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:4.40|P-value:1.11E-5 ID:SGTC_686|Compound:1319-0111|FD-Score:-3.85|P-value:8.76E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:3.45|P-value:4.37E-4 ID:SGTC_772|Compound:k072-0172|FD-Score:3.40|P-value:5.27E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:4.07|P-value:4.39E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:3.37|P-value:5.84E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:-3.73|P-value:1.38E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:5.16|P-value:3.06E-7 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.67|P-value:2.03E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:4.45|P-value:8.81E-6 ID:SGTC_1459|Compound:k035-0031|FD-Score:3.43|P-value:4.80E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:3.25|P-value:8.84E-4 ID:SGTC_1744|Compound:st037937|FD-Score:4.95|P-value:8.87E-7 ID:SGTC_1746|Compound:st038117|FD-Score:5.14|P-value:3.38E-7 ID:SGTC_1752|Compound:st044819|FD-Score:4.00|P-value:5.74E-5 ID:SGTC_1897|Compound:st060201|FD-Score:4.15|P-value:3.15E-5 ID:SGTC_2416|Compound:tofa|FD-Score:5.80|P-value:1.07E-8 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:3.49|P-value:3.88E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:3.88|P-value:8.98E-5 ID:SGTC_2516|Compound:gossypol|FD-Score:-3.46|P-value:3.67E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:4.38|P-value:1.19E-5 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:4.16|P-value:2.94E-5 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.81|P-value:1.18E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:-3.23|P-value:8.10E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:-4.13|P-value:2.87E-5 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-3.79|P-value:1.07E-4 ID:SGTC_2782|Compound:7736369|FD-Score:3.92|P-value:7.87E-5 ID:SGTC_2785|Compound:7756463|FD-Score:3.38|P-value:5.67E-4 ID:SGTC_2813|Compound:7971799|FD-Score:3.22|P-value:9.73E-4 ID:SGTC_2887|Compound:9044784|FD-Score:3.79|P-value:1.30E-4 ID:SGTC_2947|Compound:9072639|FD-Score:4.88|P-value:1.20E-6 ID:SGTC_2949|Compound:9072542|FD-Score:6.77|P-value:3.05E-11 ID:SGTC_2950|Compound:9072895|FD-Score:3.94|P-value:7.23E-5 ID:SGTC_2951|Compound:9072950|FD-Score:3.23|P-value:9.23E-4 ID:SGTC_3052|Compound:9092717|FD-Score:3.49|P-value:3.89E-4 ID:SGTC_3056|Compound:9094900|FD-Score:3.64|P-value:2.21E-4 ID:SGTC_3106|Compound:9121286|FD-Score:3.97|P-value:6.56E-5 ID:SGTC_3131|Compound:9127911|FD-Score:-4.97|P-value:6.33E-7 ID:SGTC_3136|Compound:9095672|FD-Score:4.15|P-value:3.10E-5 ID:SGTC_3182|Compound:9108849|FD-Score:-3.20|P-value:8.99E-4 ID:SGTC_3245|Compound:9134821|FD-Score:3.77|P-value:1.38E-4 ID:SGTC_45|Compound:0kpi-0036|FD-Score:3.39|P-value:5.51E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:4.09|P-value:3.99E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:3.41|P-value:5.08E-4 ID:SGTC_274|Compound:sirtinol|FD-Score:-3.31|P-value:6.22E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:-3.51|P-value:3.09E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:4.40|P-value:1.11E-5 ID:SGTC_686|Compound:1319-0111|FD-Score:-3.85|P-value:8.76E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:3.45|P-value:4.37E-4 ID:SGTC_772|Compound:k072-0172|FD-Score:3.40|P-value:5.27E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:4.07|P-value:4.39E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:3.37|P-value:5.84E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:-3.73|P-value:1.38E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:5.16|P-value:3.06E-7 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.67|P-value:2.03E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:4.45|P-value:8.81E-6 ID:SGTC_1459|Compound:k035-0031|FD-Score:3.43|P-value:4.80E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:3.25|P-value:8.84E-4 ID:SGTC_1744|Compound:st037937|FD-Score:4.95|P-value:8.87E-7 ID:SGTC_1746|Compound:st038117|FD-Score:5.14|P-value:3.38E-7 ID:SGTC_1752|Compound:st044819|FD-Score:4.00|P-value:5.74E-5 ID:SGTC_1897|Compound:st060201|FD-Score:4.15|P-value:3.15E-5 ID:SGTC_2416|Compound:tofa|FD-Score:5.80|P-value:1.07E-8 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:3.49|P-value:3.88E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:3.88|P-value:8.98E-5 ID:SGTC_2516|Compound:gossypol|FD-Score:-3.46|P-value:3.67E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:4.38|P-value:1.19E-5 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:4.16|P-value:2.94E-5 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.81|P-value:1.18E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:-3.23|P-value:8.10E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:-4.13|P-value:2.87E-5 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-3.79|P-value:1.07E-4 ID:SGTC_2782|Compound:7736369|FD-Score:3.92|P-value:7.87E-5 ID:SGTC_2785|Compound:7756463|FD-Score:3.38|P-value:5.67E-4 ID:SGTC_2813|Compound:7971799|FD-Score:3.22|P-value:9.73E-4 ID:SGTC_2887|Compound:9044784|FD-Score:3.79|P-value:1.30E-4 ID:SGTC_2947|Compound:9072639|FD-Score:4.88|P-value:1.20E-6 ID:SGTC_2949|Compound:9072542|FD-Score:6.77|P-value:3.05E-11 ID:SGTC_2950|Compound:9072895|FD-Score:3.94|P-value:7.23E-5 ID:SGTC_2951|Compound:9072950|FD-Score:3.23|P-value:9.23E-4 ID:SGTC_3052|Compound:9092717|FD-Score:3.49|P-value:3.89E-4 ID:SGTC_3056|Compound:9094900|FD-Score:3.64|P-value:2.21E-4 ID:SGTC_3106|Compound:9121286|FD-Score:3.97|P-value:6.56E-5 ID:SGTC_3131|Compound:9127911|FD-Score:-4.97|P-value:6.33E-7 ID:SGTC_3136|Compound:9095672|FD-Score:4.15|P-value:3.10E-5 ID:SGTC_3182|Compound:9108849|FD-Score:-3.20|P-value:8.99E-4 ID:SGTC_3245|Compound:9134821|FD-Score:3.77|P-value:1.38E-4

Top fitness defect scores for YKL053C-A deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2949 9072542 6.77 2.2 µM 3.05E-11 TSC3-RPN4 3 6.61E-12
2 SGTC_2416 tofa 5.80 880.0 nM 1.07E-8 sphingolipid biosynthesis & PDR1 19 3.40E-9
3 SGTC_1372 2752-0194 5.16 270.0 µM 3.06E-7 7 1.21E-7
4 SGTC_1746 st038117 5.14 42.0 µM 3.38E-7 14 1.34E-7
5 SGTC_1744 st037937 4.95 38.9 µM 8.87E-7 7 3.75E-7
6 SGTC_2947 9072639 4.88 7.7 µM 1.20E-6 tubulin folding & SWR complex 19 5.17E-7
7 SGTC_1450 4112-4260 4.45 37.6 µM 8.81E-6 calcium & mitochondrial duress 18 4.33E-6
8 SGTC_413 propylparaben 4.40 100.0 µM 1.11E-5 amide catabolism 13 5.53E-6
9 SGTC_2553 4-methylesculetin 4.38 85.1 µM 1.19E-5 13 5.97E-6
10 SGTC_2562 3',7'-dimethoxyflavone 4.16 64.9 µM 2.94E-5 25 1.56E-5
11 SGTC_3136 9095672 4.15 49.5 µM 3.10E-5 21 1.65E-5
12 SGTC_1897 st060201 4.15 15.0 µM 3.15E-5 mitochondrial processes 15 1.68E-5
13 SGTC_131 0141-0277 4.09 33.9 µM 3.99E-5 32 2.16E-5
14 SGTC_923 1900-2434 4.07 73.3 µM 4.39E-5 RNA processing & uracil transport 34 2.39E-5
15 SGTC_1752 st044819 4.00 83.0 µM 5.74E-5 24 3.18E-5
16 SGTC_3106 9121286 3.97 49.5 µM 6.56E-5 11 3.67E-5
17 SGTC_2950 9072895 3.94 21.5 µM 7.23E-5 38 4.07E-5
18 SGTC_2782 7736369 3.92 71.4 µM 7.87E-5 42 4.46E-5
19 SGTC_2515 chrysin 3.88 20.8 µM 8.98E-5 RPP1 & pyrimidine depletion 8 5.13E-5
20 SGTC_2603 homopterocarpin 3.81 100.0 µM 1.18E-4 47 6.87E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.172 9.85E-24 YKR028W SAP190 hom Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
0.101 5.34E-9 YER023W PRO3 het Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis
0.098 1.15E-8 YIR032C DAL3 hom Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression
0.088 3.05E-7 YDR285W ZIP1 hom Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate
0.087 4.76E-7 YJL142C_d IRC9_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci
0.083 1.81E-6 YCR007C_p YCR007C_p hom Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene
0.081 2.78E-6 YHR210C_p YHR210C_p hom Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions
0.080 3.43E-6 YJR023C_d YJR023C_d het Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing
0.080 3.63E-6 YPL099C AIM43 hom Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
0.075 1.37E-5 YER116C SLX8 hom Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress
0.075 1.50E-5 YBL058W SHP1 hom UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate
0.073 2.08E-5 YHR156C LIN1 hom Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication
0.071 3.76E-5 YMR011W HXT2 hom High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose
0.071 3.86E-5 YNL095C_p YNL095C_p hom Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication
0.071 4.10E-5 YMR023C MSS1 hom Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3