YKL053W_d

Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL053W / YKL053W

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:10.10|P-value:3.09E-25 ID:SGTC_146|Compound:3448-8650|FD-Score:3.11|P-value:6.58E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:3.71|P-value:6.54E-5 ID:SGTC_341|Compound:1486-1430|FD-Score:3.24|P-value:4.13E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:-3.82|P-value:5.87E-5 ID:SGTC_360|Compound:0957-0399|FD-Score:3.50|P-value:1.55E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:3.54|P-value:1.29E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:3.94|P-value:2.43E-5 ID:SGTC_376|Compound:3970-0862|FD-Score:3.96|P-value:2.23E-5 ID:SGTC_379|Compound:3970-0972|FD-Score:7.18|P-value:9.51E-14 ID:SGTC_381|Compound:3461-2219|FD-Score:3.22|P-value:4.48E-4 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.36|P-value:2.62E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:3.24|P-value:4.05E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:6.02|P-value:3.17E-10 ID:SGTC_634|Compound:1611-4317|FD-Score:3.60|P-value:1.03E-4 ID:SGTC_737|Compound:1950-0117|FD-Score:3.83|P-value:4.00E-5 ID:SGTC_751|Compound:0833-0568|FD-Score:3.20|P-value:4.68E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:8.05|P-value:7.95E-17 ID:SGTC_995|Compound:3931-2021|FD-Score:3.30|P-value:3.30E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-4.31|P-value:7.04E-6 ID:SGTC_1122|Compound:capsaicin|FD-Score:3.18|P-value:5.10E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.00|P-value:9.60E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.61|P-value:9.81E-5 ID:SGTC_1399|Compound:3562-5772|FD-Score:4.82|P-value:3.55E-7 ID:SGTC_1417|Compound:3966-0327|FD-Score:3.89|P-value:3.12E-5 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:-4.39|P-value:4.67E-6 ID:SGTC_1540|Compound:guanosine|FD-Score:3.16|P-value:5.41E-4 ID:SGTC_1547|Compound:harmine|FD-Score:-3.77|P-value:7.17E-5 ID:SGTC_1754|Compound:st042065|FD-Score:3.16|P-value:5.44E-4 ID:SGTC_1766|Compound:st045414|FD-Score:3.66|P-value:8.20E-5 ID:SGTC_1944|Compound:st076371|FD-Score:3.17|P-value:5.30E-4 ID:SGTC_1959|Compound:st077091|FD-Score:6.14|P-value:1.49E-10 ID:SGTC_2084|Compound:5306078|FD-Score:3.48|P-value:1.69E-4 ID:SGTC_2097|Compound:5376186|FD-Score:3.71|P-value:6.53E-5 ID:SGTC_2121|Compound:5257820|FD-Score:3.37|P-value:2.53E-4 ID:SGTC_2156|Compound:5649887|FD-Score:4.23|P-value:6.67E-6 ID:SGTC_2185|Compound:6113692|FD-Score:3.26|P-value:3.86E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.27|P-value:3.63E-4 ID:SGTC_2347|Compound:9049783|FD-Score:3.03|P-value:8.76E-4 ID:SGTC_2426|Compound:5582046|FD-Score:3.61|P-value:9.91E-5 ID:SGTC_2462|Compound:5162487|FD-Score:3.52|P-value:1.43E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:3.93|P-value:2.61E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:3.18|P-value:5.14E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:4.41|P-value:2.81E-6 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.52|P-value:1.42E-4 ID:SGTC_2686|Compound:alizarin|FD-Score:4.68|P-value:7.46E-7 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.70|P-value:1.48E-15 ID:SGTC_2691|Compound:st077356|FD-Score:4.06|P-value:1.45E-5 ID:SGTC_2798|Compound:7813637|FD-Score:3.31|P-value:3.15E-4 ID:SGTC_2880|Compound:9042983|FD-Score:3.42|P-value:2.06E-4 ID:SGTC_2954|Compound:9074818|FD-Score:4.81|P-value:3.78E-7 ID:SGTC_3061|Compound:glucosamine|FD-Score:3.02|P-value:8.96E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.10|P-value:9.09E-4 ID:SGTC_3081|Compound:9117243|FD-Score:3.38|P-value:2.40E-4 ID:SGTC_3091|Compound:9116372|FD-Score:-4.25|P-value:9.14E-6 ID:SGTC_3209|Compound:9113616|FD-Score:4.90|P-value:2.34E-7 ID:SGTC_3282|Compound:9108498|FD-Score:-3.59|P-value:1.49E-4 ID:SGTC_3311|Compound:9131146|FD-Score:3.13|P-value:6.13E-4 ID:SGTC_3321|Compound:9137793|FD-Score:3.20|P-value:4.85E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:10.10|P-value:3.09E-25 ID:SGTC_146|Compound:3448-8650|FD-Score:3.11|P-value:6.58E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:3.71|P-value:6.54E-5 ID:SGTC_341|Compound:1486-1430|FD-Score:3.24|P-value:4.13E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:-3.82|P-value:5.87E-5 ID:SGTC_360|Compound:0957-0399|FD-Score:3.50|P-value:1.55E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:3.54|P-value:1.29E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:3.94|P-value:2.43E-5 ID:SGTC_376|Compound:3970-0862|FD-Score:3.96|P-value:2.23E-5 ID:SGTC_379|Compound:3970-0972|FD-Score:7.18|P-value:9.51E-14 ID:SGTC_381|Compound:3461-2219|FD-Score:3.22|P-value:4.48E-4 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.36|P-value:2.62E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:3.24|P-value:4.05E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:6.02|P-value:3.17E-10 ID:SGTC_634|Compound:1611-4317|FD-Score:3.60|P-value:1.03E-4 ID:SGTC_737|Compound:1950-0117|FD-Score:3.83|P-value:4.00E-5 ID:SGTC_751|Compound:0833-0568|FD-Score:3.20|P-value:4.68E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:8.05|P-value:7.95E-17 ID:SGTC_995|Compound:3931-2021|FD-Score:3.30|P-value:3.30E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-4.31|P-value:7.04E-6 ID:SGTC_1122|Compound:capsaicin|FD-Score:3.18|P-value:5.10E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.00|P-value:9.60E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.61|P-value:9.81E-5 ID:SGTC_1399|Compound:3562-5772|FD-Score:4.82|P-value:3.55E-7 ID:SGTC_1417|Compound:3966-0327|FD-Score:3.89|P-value:3.12E-5 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:-4.39|P-value:4.67E-6 ID:SGTC_1540|Compound:guanosine|FD-Score:3.16|P-value:5.41E-4 ID:SGTC_1547|Compound:harmine|FD-Score:-3.77|P-value:7.17E-5 ID:SGTC_1754|Compound:st042065|FD-Score:3.16|P-value:5.44E-4 ID:SGTC_1766|Compound:st045414|FD-Score:3.66|P-value:8.20E-5 ID:SGTC_1944|Compound:st076371|FD-Score:3.17|P-value:5.30E-4 ID:SGTC_1959|Compound:st077091|FD-Score:6.14|P-value:1.49E-10 ID:SGTC_2084|Compound:5306078|FD-Score:3.48|P-value:1.69E-4 ID:SGTC_2097|Compound:5376186|FD-Score:3.71|P-value:6.53E-5 ID:SGTC_2121|Compound:5257820|FD-Score:3.37|P-value:2.53E-4 ID:SGTC_2156|Compound:5649887|FD-Score:4.23|P-value:6.67E-6 ID:SGTC_2185|Compound:6113692|FD-Score:3.26|P-value:3.86E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.27|P-value:3.63E-4 ID:SGTC_2347|Compound:9049783|FD-Score:3.03|P-value:8.76E-4 ID:SGTC_2426|Compound:5582046|FD-Score:3.61|P-value:9.91E-5 ID:SGTC_2462|Compound:5162487|FD-Score:3.52|P-value:1.43E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:3.93|P-value:2.61E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:3.18|P-value:5.14E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:4.41|P-value:2.81E-6 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.52|P-value:1.42E-4 ID:SGTC_2686|Compound:alizarin|FD-Score:4.68|P-value:7.46E-7 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.70|P-value:1.48E-15 ID:SGTC_2691|Compound:st077356|FD-Score:4.06|P-value:1.45E-5 ID:SGTC_2798|Compound:7813637|FD-Score:3.31|P-value:3.15E-4 ID:SGTC_2880|Compound:9042983|FD-Score:3.42|P-value:2.06E-4 ID:SGTC_2954|Compound:9074818|FD-Score:4.81|P-value:3.78E-7 ID:SGTC_3061|Compound:glucosamine|FD-Score:3.02|P-value:8.96E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.10|P-value:9.09E-4 ID:SGTC_3081|Compound:9117243|FD-Score:3.38|P-value:2.40E-4 ID:SGTC_3091|Compound:9116372|FD-Score:-4.25|P-value:9.14E-6 ID:SGTC_3209|Compound:9113616|FD-Score:4.90|P-value:2.34E-7 ID:SGTC_3282|Compound:9108498|FD-Score:-3.59|P-value:1.49E-4 ID:SGTC_3311|Compound:9131146|FD-Score:3.13|P-value:6.13E-4 ID:SGTC_3321|Compound:9137793|FD-Score:3.20|P-value:4.85E-4

Top fitness defect scores for YKL053W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 10.10 9.0 µM 3.09E-25 cell wall 75 3.44E-24
2 SGTC_916 4466-0038 8.05 42.0 nM 7.95E-17 cell wall 12 4.02E-16
3 SGTC_2688 kanamycin b 7.70 96.7 µM 1.48E-15 calcium & mitochondrial duress 79 6.62E-15
4 SGTC_379 3970-0972 7.18 5.9 µM 9.51E-14 tubulin folding & SWR complex 3 3.59E-13
5 SGTC_1959 st077091 6.14 55.8 µM 1.49E-10 RPP1 & pyrimidine depletion 7 4.14E-10
6 SGTC_514 4466-0038 6.02 2.6 nM 3.17E-10 cell wall 72 8.50E-10
7 SGTC_3209 9113616 4.90 49.5 µM 2.34E-7 8 4.72E-7
8 SGTC_1399 3562-5772 4.82 25.0 µM 3.55E-7 RPP1 & pyrimidine depletion 7 7.05E-7
9 SGTC_2954 9074818 4.81 52.7 µM 3.78E-7 22 7.49E-7
10 SGTC_2686 alizarin 4.68 95.4 µM 7.46E-7 RPP1 & pyrimidine depletion 22 1.43E-6
11 SGTC_2626 ethyl orsellinate 4.41 100.0 µM 2.81E-6 25 5.10E-6
12 SGTC_2156 5649887 4.23 115.0 µM 6.67E-6 RPP1 & pyrimidine depletion 7 1.17E-5
13 SGTC_2691 st077356 4.06 60.2 µM 1.45E-5 25 2.45E-5
14 SGTC_376 3970-0862 3.96 59.1 µM 2.23E-5 18 3.69E-5
15 SGTC_373 0844-0104 3.94 25.9 µM 2.43E-5 ERAD & cell cycle 46 4.00E-5
16 SGTC_2507 purpurin 3.93 79.7 µM 2.61E-5 copper-dependent oxidative stress 36 4.28E-5
17 SGTC_1417 3966-0327 3.89 90.4 µM 3.12E-5 17 5.10E-5
18 SGTC_737 1950-0117 3.83 57.9 µM 4.00E-5 29 6.45E-5
19 SGTC_2097 5376186 3.71 200.0 µM 6.53E-5 30 1.03E-4
20 SGTC_304 1310-0298 3.71 97.0 µM 6.54E-5 cell wall 70 1.03E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.353 1.09E-98 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.316 7.39E-79 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.297 5.19E-69 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.293 1.17E-67 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.261 1.91E-53 YKL052C ASK1 het Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
0.259 1.90E-52 YML119W_p YML119W_p hom Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate
0.252 1.24E-49 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.251 2.36E-49 YML117W NAB6 hom Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.243 3.17E-46 YNL288W CAF40 hom Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p
0.238 1.30E-44 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.238 1.32E-44 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.236 9.82E-44 YDL213C NOP6 hom rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes
0.233 1.91E-42 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.226 3.03E-40 YMR036C MIH1 hom Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.217 6.14E-37 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi