HIPHOP chemogenomics database

YKL073W / LHS1

Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway

Zygosity: Homozygous strain

fixedexpanded
Profile for YKL073W / LHS1

Click on Significant Values for Screen Details

Top fitness defect scores for YKL073W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1163	k060-0048	6.74	49.9 µM	5.17E-10		2	7.96E-12
2	SGTC_1675	st018485	6.61	24.5 µM	1.09E-9	60S ribosome export	4	1.96E-11
3	SGTC_2348	9022543	6.18	33.4 µM	1.12E-8	mitochondrial processes	6	3.29E-10
4	SGTC_2822	7994718	6.15	71.4 µM	1.25E-8		5	3.78E-10
5	SGTC_2036	5160441	5.40	105.0 µM	5.01E-7	NEO1	48	3.29E-8
6	SGTC_997	3996-0078	5.10	137.0 µM	1.99E-6		6	1.74E-7
7	SGTC_2976	9009005	4.97	19.5 µM	3.47E-6		19	3.42E-7
8	SGTC_1873	rapamycin	4.83	2.0 nM	6.05E-6		52	6.69E-7
9	SGTC_2737	bleomycin	4.72	30.5 nM	9.62E-6	Golgi	66	1.17E-6
10	SGTC_632	0781-2353	4.68	4.7 µM	1.15E-5	Golgi	21	1.46E-6
11	SGTC_2526	patulin	4.66	37.6 µM	1.24E-5		56	1.59E-6
12	SGTC_637	1181-0519	4.66	7.3 µM	1.25E-5	fatty acid desaturase (OLE1)	78	1.61E-6
13	SGTC_951	1171-0579	4.65	82.7 µM	1.30E-5	cell wall	51	1.69E-6
14	SGTC_2006	4011487	4.59	200.0 µM	1.66E-5	endomembrane recycling	65	2.27E-6
15	SGTC_2520	obtusaquinone	4.57	4.7 µM	1.80E-5		8	2.49E-6
16	SGTC_2320	7373003	4.45	200.0 µM	2.86E-5		16	4.38E-6
17	SGTC_568	1193-0097	4.43	7.5 µM	3.08E-5	ERAD & cell cycle	116	4.78E-6
18	SGTC_2037	5169046	4.27	200.0 µM	5.56E-5	60S ribosome export	10	9.75E-6
19	SGTC_923	1900-2434	4.23	73.3 µM	6.50E-5	RNA processing & uracil transport	29	1.18E-5
20	SGTC_1014	4130-3005	4.18	69.3 µM	7.74E-5		12	1.45E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.137	1.43E-15	YDL140C	RPO21	het	RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
0.129	5.19E-14	YDL133W	SRF1	hom	Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients
0.115	2.24E-11	YBL022C	PIM1	hom	ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
0.110	1.42E-10	YHR007C	ERG11	het	Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
0.106	6.13E-10	YOR319W	HSH49	het	U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
0.104	1.52E-9	YGR060W	ERG25	het	C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
0.104	1.82E-9	YDL077C	VAM6	hom	Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
0.100	6.53E-9	YML037C_p	YML037C_p	hom	Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.099	9.03E-9	YCR079W	PTC6	hom	Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase
0.099	1.02E-8	YLR380W	CSR1	hom	Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
0.099	1.07E-8	YDL192W	ARF1	hom	ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.096	2.20E-8	YOL144W	NOP8	het	Nucleolar protein required for 60S ribosomal subunit biogenesis
0.096	2.81E-8	YNL190W_p	YNL190W_p	hom	Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site
0.096	2.85E-8	YHR149C	SKG6	hom	Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.094	4.67E-8	YKL039W	PTM1	hom	Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication