YKL073W / LHS1

Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL073W / LHS1

Click on Significant Values for Screen Details ID:SGTC_18|Compound:0111-0177|FD-Score:4.02|P-value:1.36E-4 ID:SGTC_51|Compound:1013-0126|FD-Score:-3.52|P-value:6.73E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-3.62|P-value:4.95E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:4.11|P-value:1.01E-4 ID:SGTC_470|Compound:aa-861|FD-Score:-4.00|P-value:1.39E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:3.81|P-value:2.87E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.43|P-value:3.08E-5 ID:SGTC_632|Compound:0781-2353|FD-Score:4.68|P-value:1.15E-5 ID:SGTC_637|Compound:1181-0519|FD-Score:4.66|P-value:1.25E-5 ID:SGTC_841|Compound:0251-0215|FD-Score:3.75|P-value:3.43E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:3.45|P-value:9.06E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:4.23|P-value:6.50E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:3.51|P-value:7.39E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:4.65|P-value:1.30E-5 ID:SGTC_994|Compound:3910-0338|FD-Score:3.72|P-value:3.87E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:5.10|P-value:1.99E-6 ID:SGTC_1014|Compound:4130-3005|FD-Score:4.18|P-value:7.74E-5 ID:SGTC_1157|Compound:4092-0394|FD-Score:-3.48|P-value:7.82E-4 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.80|P-value:2.88E-4 ID:SGTC_1163|Compound:k060-0048|FD-Score:6.74|P-value:5.17E-10 ID:SGTC_1167|Compound:k781-0610|FD-Score:3.78|P-value:3.17E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.65|P-value:4.88E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:3.57|P-value:6.26E-4 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.91|P-value:2.01E-4 ID:SGTC_1324|Compound:1418-0026|FD-Score:3.58|P-value:5.99E-4 ID:SGTC_1466|Compound:k213-0079|FD-Score:4.17|P-value:8.12E-5 ID:SGTC_1675|Compound:st018485|FD-Score:6.61|P-value:1.09E-9 ID:SGTC_1873|Compound:rapamycin|FD-Score:4.83|P-value:6.05E-6 ID:SGTC_2006|Compound:4011487|FD-Score:4.59|P-value:1.66E-5 ID:SGTC_2030|Compound:5175110|FD-Score:-4.65|P-value:1.17E-5 ID:SGTC_2034|Compound:5197015|FD-Score:-4.05|P-value:1.14E-4 ID:SGTC_2036|Compound:5160441|FD-Score:5.40|P-value:5.01E-7 ID:SGTC_2037|Compound:5169046|FD-Score:4.27|P-value:5.56E-5 ID:SGTC_2089|Compound:5281248|FD-Score:4.12|P-value:9.56E-5 ID:SGTC_2136|Compound:5328288|FD-Score:3.42|P-value:9.96E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.66|P-value:4.70E-4 ID:SGTC_2320|Compound:7373003|FD-Score:4.45|P-value:2.86E-5 ID:SGTC_2348|Compound:9022543|FD-Score:6.18|P-value:1.12E-8 ID:SGTC_2397|Compound:5161689|FD-Score:-4.45|P-value:2.63E-5 ID:SGTC_2400|Compound:5203494|FD-Score:-3.76|P-value:3.18E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:3.96|P-value:1.72E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:4.57|P-value:1.80E-5 ID:SGTC_2526|Compound:patulin|FD-Score:4.66|P-value:1.24E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.82|P-value:2.78E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:4.15|P-value:8.52E-5 ID:SGTC_2663|Compound:amphotericin b|FD-Score:4.03|P-value:1.34E-4 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.83|P-value:2.61E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:4.72|P-value:9.62E-6 ID:SGTC_2822|Compound:7994718|FD-Score:6.15|P-value:1.25E-8 ID:SGTC_2932|Compound:9016711|FD-Score:3.56|P-value:6.35E-4 ID:SGTC_2941|Compound:9056145|FD-Score:-4.45|P-value:2.58E-5 ID:SGTC_2976|Compound:9009005|FD-Score:4.97|P-value:3.47E-6 ID:SGTC_3049|Compound:9092223|FD-Score:4.13|P-value:9.26E-5 ID:SGTC_3149|Compound:9097855|FD-Score:-3.50|P-value:7.37E-4 ID:SGTC_3249|Compound:9135183|FD-Score:-3.42|P-value:9.34E-4 ID:SGTC_3286|Compound:9117135|FD-Score:-3.97|P-value:1.51E-4 ID:SGTC_18|Compound:0111-0177|FD-Score:4.02|P-value:1.36E-4 ID:SGTC_51|Compound:1013-0126|FD-Score:-3.52|P-value:6.73E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-3.62|P-value:4.95E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:4.11|P-value:1.01E-4 ID:SGTC_470|Compound:aa-861|FD-Score:-4.00|P-value:1.39E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:3.81|P-value:2.87E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.43|P-value:3.08E-5 ID:SGTC_632|Compound:0781-2353|FD-Score:4.68|P-value:1.15E-5 ID:SGTC_637|Compound:1181-0519|FD-Score:4.66|P-value:1.25E-5 ID:SGTC_841|Compound:0251-0215|FD-Score:3.75|P-value:3.43E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:3.45|P-value:9.06E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:4.23|P-value:6.50E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:3.51|P-value:7.39E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:4.65|P-value:1.30E-5 ID:SGTC_994|Compound:3910-0338|FD-Score:3.72|P-value:3.87E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:5.10|P-value:1.99E-6 ID:SGTC_1014|Compound:4130-3005|FD-Score:4.18|P-value:7.74E-5 ID:SGTC_1157|Compound:4092-0394|FD-Score:-3.48|P-value:7.82E-4 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.80|P-value:2.88E-4 ID:SGTC_1163|Compound:k060-0048|FD-Score:6.74|P-value:5.17E-10 ID:SGTC_1167|Compound:k781-0610|FD-Score:3.78|P-value:3.17E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.65|P-value:4.88E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:3.57|P-value:6.26E-4 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.91|P-value:2.01E-4 ID:SGTC_1324|Compound:1418-0026|FD-Score:3.58|P-value:5.99E-4 ID:SGTC_1466|Compound:k213-0079|FD-Score:4.17|P-value:8.12E-5 ID:SGTC_1675|Compound:st018485|FD-Score:6.61|P-value:1.09E-9 ID:SGTC_1873|Compound:rapamycin|FD-Score:4.83|P-value:6.05E-6 ID:SGTC_2006|Compound:4011487|FD-Score:4.59|P-value:1.66E-5 ID:SGTC_2030|Compound:5175110|FD-Score:-4.65|P-value:1.17E-5 ID:SGTC_2034|Compound:5197015|FD-Score:-4.05|P-value:1.14E-4 ID:SGTC_2036|Compound:5160441|FD-Score:5.40|P-value:5.01E-7 ID:SGTC_2037|Compound:5169046|FD-Score:4.27|P-value:5.56E-5 ID:SGTC_2089|Compound:5281248|FD-Score:4.12|P-value:9.56E-5 ID:SGTC_2136|Compound:5328288|FD-Score:3.42|P-value:9.96E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.66|P-value:4.70E-4 ID:SGTC_2320|Compound:7373003|FD-Score:4.45|P-value:2.86E-5 ID:SGTC_2348|Compound:9022543|FD-Score:6.18|P-value:1.12E-8 ID:SGTC_2397|Compound:5161689|FD-Score:-4.45|P-value:2.63E-5 ID:SGTC_2400|Compound:5203494|FD-Score:-3.76|P-value:3.18E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:3.96|P-value:1.72E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:4.57|P-value:1.80E-5 ID:SGTC_2526|Compound:patulin|FD-Score:4.66|P-value:1.24E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.82|P-value:2.78E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:4.15|P-value:8.52E-5 ID:SGTC_2663|Compound:amphotericin b|FD-Score:4.03|P-value:1.34E-4 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.83|P-value:2.61E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:4.72|P-value:9.62E-6 ID:SGTC_2822|Compound:7994718|FD-Score:6.15|P-value:1.25E-8 ID:SGTC_2932|Compound:9016711|FD-Score:3.56|P-value:6.35E-4 ID:SGTC_2941|Compound:9056145|FD-Score:-4.45|P-value:2.58E-5 ID:SGTC_2976|Compound:9009005|FD-Score:4.97|P-value:3.47E-6 ID:SGTC_3049|Compound:9092223|FD-Score:4.13|P-value:9.26E-5 ID:SGTC_3149|Compound:9097855|FD-Score:-3.50|P-value:7.37E-4 ID:SGTC_3249|Compound:9135183|FD-Score:-3.42|P-value:9.34E-4 ID:SGTC_3286|Compound:9117135|FD-Score:-3.97|P-value:1.51E-4

Top fitness defect scores for YKL073W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1163 k060-0048 6.74 49.9 µM 5.17E-10 2 7.96E-12
2 SGTC_1675 st018485 6.61 24.5 µM 1.09E-9 60S ribosome export 4 1.96E-11
3 SGTC_2348 9022543 6.18 33.4 µM 1.12E-8 mitochondrial processes 6 3.29E-10
4 SGTC_2822 7994718 6.15 71.4 µM 1.25E-8 5 3.78E-10
5 SGTC_2036 5160441 5.40 105.0 µM 5.01E-7 NEO1 48 3.29E-8
6 SGTC_997 3996-0078 5.10 137.0 µM 1.99E-6 6 1.74E-7
7 SGTC_2976 9009005 4.97 19.5 µM 3.47E-6 19 3.42E-7
8 SGTC_1873 rapamycin 4.83 2.0 nM 6.05E-6 52 6.69E-7
9 SGTC_2737 bleomycin 4.72 30.5 nM 9.62E-6 Golgi 66 1.17E-6
10 SGTC_632 0781-2353 4.68 4.7 µM 1.15E-5 Golgi 21 1.46E-6
11 SGTC_2526 patulin 4.66 37.6 µM 1.24E-5 56 1.59E-6
12 SGTC_637 1181-0519 4.66 7.3 µM 1.25E-5 fatty acid desaturase (OLE1) 78 1.61E-6
13 SGTC_951 1171-0579 4.65 82.7 µM 1.30E-5 cell wall 51 1.69E-6
14 SGTC_2006 4011487 4.59 200.0 µM 1.66E-5 endomembrane recycling 65 2.27E-6
15 SGTC_2520 obtusaquinone 4.57 4.7 µM 1.80E-5 8 2.49E-6
16 SGTC_2320 7373003 4.45 200.0 µM 2.86E-5 16 4.38E-6
17 SGTC_568 1193-0097 4.43 7.5 µM 3.08E-5 ERAD & cell cycle 116 4.78E-6
18 SGTC_2037 5169046 4.27 200.0 µM 5.56E-5 60S ribosome export 10 9.75E-6
19 SGTC_923 1900-2434 4.23 73.3 µM 6.50E-5 RNA processing & uracil transport 29 1.18E-5
20 SGTC_1014 4130-3005 4.18 69.3 µM 7.74E-5 12 1.45E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.137 1.43E-15 YDL140C RPO21 het RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
0.129 5.19E-14 YDL133W SRF1 hom Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients
0.115 2.24E-11 YBL022C PIM1 hom ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
0.110 1.42E-10 YHR007C ERG11 het Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
0.106 6.13E-10 YOR319W HSH49 het U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
0.104 1.52E-9 YGR060W ERG25 het C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
0.104 1.82E-9 YDL077C VAM6 hom Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
0.100 6.53E-9 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.099 9.03E-9 YCR079W PTC6 hom Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase
0.099 1.02E-8 YLR380W CSR1 hom Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
0.099 1.07E-8 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.096 2.20E-8 YOL144W NOP8 het Nucleolar protein required for 60S ribosomal subunit biogenesis
0.096 2.81E-8 YNL190W_p YNL190W_p hom Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site
0.096 2.85E-8 YHR149C SKG6 hom Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.094 4.67E-8 YKL039W PTM1 hom Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication