YKL075C_p

Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL075C / YKL075C

Click on Significant Values for Screen Details ID:SGTC_40|Compound:0327-0103|FD-Score:6.42|P-value:2.81E-10 ID:SGTC_180|Compound:1486-1293|FD-Score:4.72|P-value:2.65E-6 ID:SGTC_398|Compound:bronopol|FD-Score:6.49|P-value:1.85E-10 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.91|P-value:8.21E-5 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-3.94|P-value:6.80E-5 ID:SGTC_478|Compound:N-arachidonylglycine|FD-Score:3.32|P-value:6.78E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:3.28|P-value:7.82E-4 ID:SGTC_662|Compound:k832-1615|FD-Score:-3.91|P-value:7.50E-5 ID:SGTC_871|Compound:0574-0004|FD-Score:3.36|P-value:5.95E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:-4.18|P-value:2.58E-5 ID:SGTC_962|Compound:1254-0300|FD-Score:3.27|P-value:8.11E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:4.22|P-value:2.32E-5 ID:SGTC_1213|Compound:1315-0166|FD-Score:3.23|P-value:9.33E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:5.05|P-value:5.47E-7 ID:SGTC_1313|Compound:1220-0047|FD-Score:-3.41|P-value:4.68E-4 ID:SGTC_1354|Compound:1498-1681|FD-Score:3.46|P-value:4.31E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:-3.22|P-value:9.12E-4 ID:SGTC_1630|Compound:st005209|FD-Score:-3.42|P-value:4.53E-4 ID:SGTC_1632|Compound:st005650|FD-Score:4.30|P-value:1.67E-5 ID:SGTC_1638|Compound:st008370|FD-Score:-4.20|P-value:2.38E-5 ID:SGTC_1745|Compound:st081495|FD-Score:4.84|P-value:1.49E-6 ID:SGTC_2049|Compound:5262831|FD-Score:3.44|P-value:4.56E-4 ID:SGTC_2111|Compound:5486399|FD-Score:3.56|P-value:3.01E-4 ID:SGTC_2204|Compound:7233102|FD-Score:-3.33|P-value:6.15E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:5.28|P-value:1.75E-7 ID:SGTC_2439|Compound:5492030|FD-Score:-4.29|P-value:1.60E-5 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:7.20|P-value:1.77E-12 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.98|P-value:5.86E-5 ID:SGTC_2607|Compound:solasodine|FD-Score:3.62|P-value:2.41E-4 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-3.41|P-value:4.68E-4 ID:SGTC_2708|Compound:st078559|FD-Score:-3.73|P-value:1.48E-4 ID:SGTC_2961|Compound:9083290|FD-Score:3.22|P-value:9.56E-4 ID:SGTC_3192|Compound:9110362|FD-Score:3.53|P-value:3.37E-4 ID:SGTC_3199|Compound:9111318|FD-Score:-3.67|P-value:1.86E-4 ID:SGTC_3203|Compound:9113543|FD-Score:3.33|P-value:6.70E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-5.58|P-value:3.22E-8 ID:SGTC_3206|Compound:9114350|FD-Score:3.39|P-value:5.33E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:6.42|P-value:2.81E-10 ID:SGTC_180|Compound:1486-1293|FD-Score:4.72|P-value:2.65E-6 ID:SGTC_398|Compound:bronopol|FD-Score:6.49|P-value:1.85E-10 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.91|P-value:8.21E-5 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-3.94|P-value:6.80E-5 ID:SGTC_478|Compound:N-arachidonylglycine|FD-Score:3.32|P-value:6.78E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:3.28|P-value:7.82E-4 ID:SGTC_662|Compound:k832-1615|FD-Score:-3.91|P-value:7.50E-5 ID:SGTC_871|Compound:0574-0004|FD-Score:3.36|P-value:5.95E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:-4.18|P-value:2.58E-5 ID:SGTC_962|Compound:1254-0300|FD-Score:3.27|P-value:8.11E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:4.22|P-value:2.32E-5 ID:SGTC_1213|Compound:1315-0166|FD-Score:3.23|P-value:9.33E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:5.05|P-value:5.47E-7 ID:SGTC_1313|Compound:1220-0047|FD-Score:-3.41|P-value:4.68E-4 ID:SGTC_1354|Compound:1498-1681|FD-Score:3.46|P-value:4.31E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:-3.22|P-value:9.12E-4 ID:SGTC_1630|Compound:st005209|FD-Score:-3.42|P-value:4.53E-4 ID:SGTC_1632|Compound:st005650|FD-Score:4.30|P-value:1.67E-5 ID:SGTC_1638|Compound:st008370|FD-Score:-4.20|P-value:2.38E-5 ID:SGTC_1745|Compound:st081495|FD-Score:4.84|P-value:1.49E-6 ID:SGTC_2049|Compound:5262831|FD-Score:3.44|P-value:4.56E-4 ID:SGTC_2111|Compound:5486399|FD-Score:3.56|P-value:3.01E-4 ID:SGTC_2204|Compound:7233102|FD-Score:-3.33|P-value:6.15E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:5.28|P-value:1.75E-7 ID:SGTC_2439|Compound:5492030|FD-Score:-4.29|P-value:1.60E-5 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:7.20|P-value:1.77E-12 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.98|P-value:5.86E-5 ID:SGTC_2607|Compound:solasodine|FD-Score:3.62|P-value:2.41E-4 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-3.41|P-value:4.68E-4 ID:SGTC_2708|Compound:st078559|FD-Score:-3.73|P-value:1.48E-4 ID:SGTC_2961|Compound:9083290|FD-Score:3.22|P-value:9.56E-4 ID:SGTC_3192|Compound:9110362|FD-Score:3.53|P-value:3.37E-4 ID:SGTC_3199|Compound:9111318|FD-Score:-3.67|P-value:1.86E-4 ID:SGTC_3203|Compound:9113543|FD-Score:3.33|P-value:6.70E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-5.58|P-value:3.22E-8 ID:SGTC_3206|Compound:9114350|FD-Score:3.39|P-value:5.33E-4

Top fitness defect scores for YKL075C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2565 isoliquiritigenin 7.20 100.0 µM 1.77E-12 7 2.96E-13
2 SGTC_398 bronopol 6.49 33.3 µM 1.85E-10 RNA processing & uracil transport 9 4.28E-11
3 SGTC_40 0327-0103 6.42 19.8 µM 2.81E-10 4 6.70E-11
4 SGTC_2405 ticlatone 5.28 3.5 µM 1.75E-7 36 6.52E-8
5 SGTC_1308 1187-1617 5.05 41.4 µM 5.47E-7 fatty acid desaturase (OLE1) 20 2.21E-7
6 SGTC_1745 st081495 4.84 74.0 µM 1.49E-6 RNA processing & uracil transport 43 6.43E-7
7 SGTC_180 1486-1293 4.72 102.8 µM 2.65E-6 27 1.19E-6
8 SGTC_1632 st005650 4.30 90.0 µM 1.67E-5 18 8.53E-6
9 SGTC_1074 carmofur 4.22 3.8 µM 2.32E-5 exosome 15 1.21E-5
10 SGTC_467 ro 31-8220 3.91 72.8 µM 8.21E-5 calcium & mitochondrial duress 80 4.68E-5
11 SGTC_2607 solasodine 3.62 47.4 µM 2.41E-4 69 1.48E-4
12 SGTC_2111 5486399 3.56 200.0 µM 3.01E-4 64 1.87E-4
13 SGTC_3192 9110362 3.53 49.5 µM 3.37E-4 mitochondrial processes 18 2.12E-4
14 SGTC_1354 1498-1681 3.46 81.0 µM 4.31E-4 35 2.74E-4
15 SGTC_2049 5262831 3.44 67.2 µM 4.56E-4 49 2.92E-4
16 SGTC_3206 9114350 3.39 49.5 µM 5.33E-4 36 3.45E-4
17 SGTC_871 0574-0004 3.36 149.0 µM 5.95E-4 70 3.88E-4
18 SGTC_3203 9113543 3.33 49.5 µM 6.70E-4 53 4.40E-4
19 SGTC_478 N-arachidonylglycine 3.32 10.0 µM 6.78E-4 8 4.46E-4
20 SGTC_496 calpeptin 3.28 138.0 µM 7.82E-4 37 5.19E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.157 8.08E-20 YOR282W_d YOR282W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.152 9.97E-19 YNL010W_p YNL010W_p hom Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation
0.127 1.29E-13 YOR353C SOG2 het Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis
0.127 1.55E-13 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.118 8.09E-12 YHR193C EGD2 hom Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes
0.108 3.23E-10 YDR353W TRR1 het Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication
0.102 2.68E-9 YDR289C RTT103 hom Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition
0.102 3.22E-9 YKL135C APL2 hom Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress
0.100 6.14E-9 YOR151C RPB2 het RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit
0.100 6.42E-9 YNL066W SUN4 hom Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family
0.100 6.44E-9 YOL016C CMK2 hom Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication
0.096 2.50E-8 YKL161C KDX1 hom Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p
0.096 2.98E-8 YPR082C DIB1 het 17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
0.093 7.45E-8 YER074W RPS24A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication
0.093 7.93E-8 YJR124C_p YJR124C_p hom Putative protein of unknown function; expression induced under calcium shortage