YKL080W / VMA5

Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL080W / VMA5

Click on Significant Values for Screen Details ID:SGTC_39|Compound:0986-0283|FD-Score:-3.86|P-value:6.89E-5 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:3.61|P-value:5.91E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-3.68|P-value:1.35E-4 ID:SGTC_110|Compound:0850-0284|FD-Score:-3.36|P-value:4.09E-4 ID:SGTC_111|Compound:0083-0039|FD-Score:-3.79|P-value:8.99E-5 ID:SGTC_112|Compound:phenothiazine|FD-Score:3.69|P-value:4.61E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:-3.55|P-value:2.15E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:3.57|P-value:6.79E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:-4.30|P-value:1.16E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:3.48|P-value:9.13E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:5.37|P-value:4.78E-7 ID:SGTC_440|Compound:fluspirilene|FD-Score:5.48|P-value:2.78E-7 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.83|P-value:7.71E-5 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.48|P-value:2.76E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:4.09|P-value:1.09E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:3.82|P-value:2.88E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:-3.36|P-value:4.16E-4 ID:SGTC_861|Compound:0443-0273|FD-Score:-3.23|P-value:6.33E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-8.60|P-value:5.25E-17 ID:SGTC_894|Compound:0960-0238|FD-Score:-4.67|P-value:2.31E-6 ID:SGTC_896|Compound:0964-0007|FD-Score:-4.90|P-value:7.85E-7 ID:SGTC_1087|Compound:pterostilbene|FD-Score:-3.76|P-value:9.84E-5 ID:SGTC_1091|Compound:telmisartan|FD-Score:-3.21|P-value:6.88E-4 ID:SGTC_1322|Compound:1348-1070|FD-Score:-3.18|P-value:7.45E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:4.55|P-value:1.84E-5 ID:SGTC_1450|Compound:4112-4260|FD-Score:-3.37|P-value:3.99E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.16|P-value:8.11E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.20|P-value:6.94E-4 ID:SGTC_1813|Compound:st051246|FD-Score:-3.27|P-value:5.58E-4 ID:SGTC_1938|Compound:st074717|FD-Score:-3.16|P-value:8.09E-4 ID:SGTC_2175|Compound:5807670|FD-Score:-3.22|P-value:6.60E-4 ID:SGTC_2350|Compound:9023895|FD-Score:-3.78|P-value:9.18E-5 ID:SGTC_2481|Compound:5478948|FD-Score:3.77|P-value:3.50E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:-3.52|P-value:2.36E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.95|P-value:1.84E-4 ID:SGTC_2786|Compound:7743617|FD-Score:3.75|P-value:3.77E-4 ID:SGTC_2827|Compound:7993847|FD-Score:3.66|P-value:4.98E-4 ID:SGTC_2995|Compound:9067767|FD-Score:5.62|P-value:1.39E-7 ID:SGTC_3155|Compound:9099265|FD-Score:6.67|P-value:4.34E-10 ID:SGTC_3194|Compound:9111903|FD-Score:4.72|P-value:8.98E-6 ID:SGTC_3261|Compound:9137475|FD-Score:-3.48|P-value:2.69E-4 ID:SGTC_3291|Compound:9121177|FD-Score:-3.12|P-value:9.25E-4 ID:SGTC_3295|Compound:9122362|FD-Score:5.00|P-value:2.60E-6 ID:SGTC_39|Compound:0986-0283|FD-Score:-3.86|P-value:6.89E-5 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:3.61|P-value:5.91E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-3.68|P-value:1.35E-4 ID:SGTC_110|Compound:0850-0284|FD-Score:-3.36|P-value:4.09E-4 ID:SGTC_111|Compound:0083-0039|FD-Score:-3.79|P-value:8.99E-5 ID:SGTC_112|Compound:phenothiazine|FD-Score:3.69|P-value:4.61E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:-3.55|P-value:2.15E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:3.57|P-value:6.79E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:-4.30|P-value:1.16E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:3.48|P-value:9.13E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:5.37|P-value:4.78E-7 ID:SGTC_440|Compound:fluspirilene|FD-Score:5.48|P-value:2.78E-7 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.83|P-value:7.71E-5 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.48|P-value:2.76E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:4.09|P-value:1.09E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:3.82|P-value:2.88E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:-3.36|P-value:4.16E-4 ID:SGTC_861|Compound:0443-0273|FD-Score:-3.23|P-value:6.33E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-8.60|P-value:5.25E-17 ID:SGTC_894|Compound:0960-0238|FD-Score:-4.67|P-value:2.31E-6 ID:SGTC_896|Compound:0964-0007|FD-Score:-4.90|P-value:7.85E-7 ID:SGTC_1087|Compound:pterostilbene|FD-Score:-3.76|P-value:9.84E-5 ID:SGTC_1091|Compound:telmisartan|FD-Score:-3.21|P-value:6.88E-4 ID:SGTC_1322|Compound:1348-1070|FD-Score:-3.18|P-value:7.45E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:4.55|P-value:1.84E-5 ID:SGTC_1450|Compound:4112-4260|FD-Score:-3.37|P-value:3.99E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.16|P-value:8.11E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.20|P-value:6.94E-4 ID:SGTC_1813|Compound:st051246|FD-Score:-3.27|P-value:5.58E-4 ID:SGTC_1938|Compound:st074717|FD-Score:-3.16|P-value:8.09E-4 ID:SGTC_2175|Compound:5807670|FD-Score:-3.22|P-value:6.60E-4 ID:SGTC_2350|Compound:9023895|FD-Score:-3.78|P-value:9.18E-5 ID:SGTC_2481|Compound:5478948|FD-Score:3.77|P-value:3.50E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:-3.52|P-value:2.36E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.95|P-value:1.84E-4 ID:SGTC_2786|Compound:7743617|FD-Score:3.75|P-value:3.77E-4 ID:SGTC_2827|Compound:7993847|FD-Score:3.66|P-value:4.98E-4 ID:SGTC_2995|Compound:9067767|FD-Score:5.62|P-value:1.39E-7 ID:SGTC_3155|Compound:9099265|FD-Score:6.67|P-value:4.34E-10 ID:SGTC_3194|Compound:9111903|FD-Score:4.72|P-value:8.98E-6 ID:SGTC_3261|Compound:9137475|FD-Score:-3.48|P-value:2.69E-4 ID:SGTC_3291|Compound:9121177|FD-Score:-3.12|P-value:9.25E-4 ID:SGTC_3295|Compound:9122362|FD-Score:5.00|P-value:2.60E-6

Top fitness defect scores for YKL080W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3155 9099265 6.67 49.5 µM 4.34E-10 3 1.28E-11
2 SGTC_2995 9067767 5.62 71.4 µM 1.39E-7 6 9.67E-9
3 SGTC_440 fluspirilene 5.48 35.1 µM 2.78E-7 2 2.15E-8
4 SGTC_275 trichlorophene 5.37 5.7 µM 4.78E-7 mitochondrial stress 146 4.03E-8
5 SGTC_3295 9122362 5.00 53.6 µM 2.60E-6 4 2.84E-7
6 SGTC_3194 9111903 4.72 49.5 µM 8.98E-6 11 1.19E-6
7 SGTC_1380 2291-0730 4.55 164.0 µM 1.84E-5 12 2.72E-6
8 SGTC_625 k213-0058 4.09 121.0 µM 1.09E-4 translation 97 2.13E-5
9 SGTC_2603 homopterocarpin 3.95 100.0 µM 1.84E-4 38 3.92E-5
10 SGTC_633 0437-0093 3.82 97.7 µM 2.88E-4 DNA intercalators 15 6.60E-5
11 SGTC_2481 5478948 3.77 45.2 µM 3.50E-4 PDR1 41 8.27E-5
12 SGTC_2786 7743617 3.75 45.5 µM 3.77E-4 33 9.01E-5
13 SGTC_112 phenothiazine 3.69 287.2 µM 4.61E-4 unfolded protein response 17 1.14E-4
14 SGTC_2827 7993847 3.66 71.4 µM 4.98E-4 35 1.25E-4
15 SGTC_102 gallobenzophenone 3.61 83.6 µM 5.91E-4 redox potentiating 53 1.52E-4
16 SGTC_134 0269-0018 3.57 6.9 µM 6.79E-4 153 1.79E-4
17 SGTC_236 1509-0011 3.48 5.3 µM 9.13E-4 419 2.52E-4
18 SGTC_593 1203-0218 3.44 61.7 µM 0.00102 20 2.86E-4
19 SGTC_1902 st060212 3.44 70.5 µM 0.00102 21 2.88E-4
20 SGTC_627 1391-0720 3.44 175.0 µM 0.00103 28 2.89E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.156 9.51E-20 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.154 3.16E-19 YGL107C RMD9 hom Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes
0.151 1.23E-18 YBL080C PET112 hom Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog
0.143 9.40E-17 YDR231C COX20 hom Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase
0.142 1.37E-16 YIL070C MAM33 hom Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R
0.141 1.80E-16 YML129C COX14 hom Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes
0.139 6.44E-16 YPR088C SRP54 het Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain
0.137 1.77E-15 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.136 2.52E-15 YDR377W ATP17 hom Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
0.134 6.33E-15 YJL046W AIM22 hom Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss
0.132 1.35E-14 YNL252C MRPL17 hom Mitochondrial ribosomal protein of the large subunit
0.132 1.59E-14 YOR200W_d YOR200W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c
0.131 2.09E-14 YHL004W MRP4 hom Mitochondrial ribosomal protein of the small subunit
0.131 2.26E-14 YDR079W PET100 hom Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme
0.130 3.58E-14 YKL068W-A_p YKL068W-A_p hom Putative protein of unknown function; identified by homology to Ashbya gossypii