YKL081W / TEF4

Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL081W / TEF4

Click on Significant Values for Screen Details ID:SGTC_219|Compound:mitoxanthrone|FD-Score:4.52|P-value:6.19E-6 ID:SGTC_524|Compound:r073-0010|FD-Score:-4.48|P-value:6.86E-6 ID:SGTC_533|Compound:1222-0222|FD-Score:3.43|P-value:4.69E-4 ID:SGTC_569|Compound:4130-2812|FD-Score:3.70|P-value:1.79E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:-3.38|P-value:5.09E-4 ID:SGTC_835|Compound:0312-0010|FD-Score:3.70|P-value:1.76E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:-5.03|P-value:5.17E-7 ID:SGTC_866|Compound:0495-0101|FD-Score:4.10|P-value:3.77E-5 ID:SGTC_871|Compound:0574-0004|FD-Score:-4.86|P-value:1.19E-6 ID:SGTC_872|Compound:0588-0139|FD-Score:-3.44|P-value:4.13E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:4.15|P-value:3.02E-5 ID:SGTC_883|Compound:0850-0245|FD-Score:4.49|P-value:7.19E-6 ID:SGTC_1050|Compound:3232-1156|FD-Score:3.77|P-value:1.36E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:5.60|P-value:3.05E-8 ID:SGTC_1056|Compound:3448-1962|FD-Score:4.26|P-value:1.96E-5 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:3.70|P-value:1.76E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:-9.31|P-value:8.39E-20 ID:SGTC_1090|Compound:efavirenz|FD-Score:-3.52|P-value:3.13E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:3.85|P-value:9.89E-5 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.23|P-value:8.98E-4 ID:SGTC_1222|Compound:0366-0074|FD-Score:-3.37|P-value:5.17E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:3.81|P-value:1.15E-4 ID:SGTC_1673|Compound:st016611|FD-Score:3.49|P-value:3.77E-4 ID:SGTC_1786|Compound:berberine|FD-Score:3.33|P-value:6.54E-4 ID:SGTC_1904|Compound:st060225|FD-Score:4.07|P-value:4.16E-5 ID:SGTC_1906|Compound:st060206|FD-Score:-3.66|P-value:1.90E-4 ID:SGTC_1966|Compound:st070145|FD-Score:3.49|P-value:3.71E-4 ID:SGTC_2107|Compound:5363541|FD-Score:-3.53|P-value:2.96E-4 ID:SGTC_2121|Compound:5257820|FD-Score:-4.19|P-value:2.37E-5 ID:SGTC_2461|Compound:5755302|FD-Score:-3.79|P-value:1.17E-4 ID:SGTC_2486|Compound:5283202|FD-Score:4.04|P-value:4.77E-5 ID:SGTC_2623|Compound:humulene|FD-Score:3.24|P-value:8.72E-4 ID:SGTC_2628|Compound:lathosterol|FD-Score:3.23|P-value:8.99E-4 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:3.33|P-value:6.54E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-4.59|P-value:4.06E-6 ID:SGTC_2782|Compound:7736369|FD-Score:-3.29|P-value:6.94E-4 ID:SGTC_2783|Compound:7738165|FD-Score:-3.59|P-value:2.41E-4 ID:SGTC_2788|Compound:5282734|FD-Score:-8.58|P-value:4.30E-17 ID:SGTC_2805|Compound:7950783|FD-Score:4.56|P-value:5.32E-6 ID:SGTC_2834|Compound:9001755|FD-Score:-3.70|P-value:1.60E-4 ID:SGTC_2850|Compound:9015429|FD-Score:3.24|P-value:8.73E-4 ID:SGTC_2853|Compound:9015042|FD-Score:5.52|P-value:4.75E-8 ID:SGTC_2855|Compound:9016525|FD-Score:6.69|P-value:4.84E-11 ID:SGTC_2931|Compound:9014674|FD-Score:3.35|P-value:6.11E-4 ID:SGTC_2932|Compound:9016711|FD-Score:-3.32|P-value:6.32E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-4.21|P-value:2.16E-5 ID:SGTC_2961|Compound:9083290|FD-Score:5.46|P-value:6.47E-8 ID:SGTC_2988|Compound:9073640|FD-Score:-3.43|P-value:4.22E-4 ID:SGTC_2992|Compound:9074591|FD-Score:6.34|P-value:4.47E-10 ID:SGTC_2997|Compound:9067075|FD-Score:-3.59|P-value:2.41E-4 ID:SGTC_3036|Compound:9090181|FD-Score:3.53|P-value:3.21E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:4.52|P-value:6.19E-6 ID:SGTC_524|Compound:r073-0010|FD-Score:-4.48|P-value:6.86E-6 ID:SGTC_533|Compound:1222-0222|FD-Score:3.43|P-value:4.69E-4 ID:SGTC_569|Compound:4130-2812|FD-Score:3.70|P-value:1.79E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:-3.38|P-value:5.09E-4 ID:SGTC_835|Compound:0312-0010|FD-Score:3.70|P-value:1.76E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:-5.03|P-value:5.17E-7 ID:SGTC_866|Compound:0495-0101|FD-Score:4.10|P-value:3.77E-5 ID:SGTC_871|Compound:0574-0004|FD-Score:-4.86|P-value:1.19E-6 ID:SGTC_872|Compound:0588-0139|FD-Score:-3.44|P-value:4.13E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:4.15|P-value:3.02E-5 ID:SGTC_883|Compound:0850-0245|FD-Score:4.49|P-value:7.19E-6 ID:SGTC_1050|Compound:3232-1156|FD-Score:3.77|P-value:1.36E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:5.60|P-value:3.05E-8 ID:SGTC_1056|Compound:3448-1962|FD-Score:4.26|P-value:1.96E-5 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:3.70|P-value:1.76E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:-9.31|P-value:8.39E-20 ID:SGTC_1090|Compound:efavirenz|FD-Score:-3.52|P-value:3.13E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:3.85|P-value:9.89E-5 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.23|P-value:8.98E-4 ID:SGTC_1222|Compound:0366-0074|FD-Score:-3.37|P-value:5.17E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:3.81|P-value:1.15E-4 ID:SGTC_1673|Compound:st016611|FD-Score:3.49|P-value:3.77E-4 ID:SGTC_1786|Compound:berberine|FD-Score:3.33|P-value:6.54E-4 ID:SGTC_1904|Compound:st060225|FD-Score:4.07|P-value:4.16E-5 ID:SGTC_1906|Compound:st060206|FD-Score:-3.66|P-value:1.90E-4 ID:SGTC_1966|Compound:st070145|FD-Score:3.49|P-value:3.71E-4 ID:SGTC_2107|Compound:5363541|FD-Score:-3.53|P-value:2.96E-4 ID:SGTC_2121|Compound:5257820|FD-Score:-4.19|P-value:2.37E-5 ID:SGTC_2461|Compound:5755302|FD-Score:-3.79|P-value:1.17E-4 ID:SGTC_2486|Compound:5283202|FD-Score:4.04|P-value:4.77E-5 ID:SGTC_2623|Compound:humulene|FD-Score:3.24|P-value:8.72E-4 ID:SGTC_2628|Compound:lathosterol|FD-Score:3.23|P-value:8.99E-4 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:3.33|P-value:6.54E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-4.59|P-value:4.06E-6 ID:SGTC_2782|Compound:7736369|FD-Score:-3.29|P-value:6.94E-4 ID:SGTC_2783|Compound:7738165|FD-Score:-3.59|P-value:2.41E-4 ID:SGTC_2788|Compound:5282734|FD-Score:-8.58|P-value:4.30E-17 ID:SGTC_2805|Compound:7950783|FD-Score:4.56|P-value:5.32E-6 ID:SGTC_2834|Compound:9001755|FD-Score:-3.70|P-value:1.60E-4 ID:SGTC_2850|Compound:9015429|FD-Score:3.24|P-value:8.73E-4 ID:SGTC_2853|Compound:9015042|FD-Score:5.52|P-value:4.75E-8 ID:SGTC_2855|Compound:9016525|FD-Score:6.69|P-value:4.84E-11 ID:SGTC_2931|Compound:9014674|FD-Score:3.35|P-value:6.11E-4 ID:SGTC_2932|Compound:9016711|FD-Score:-3.32|P-value:6.32E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-4.21|P-value:2.16E-5 ID:SGTC_2961|Compound:9083290|FD-Score:5.46|P-value:6.47E-8 ID:SGTC_2988|Compound:9073640|FD-Score:-3.43|P-value:4.22E-4 ID:SGTC_2992|Compound:9074591|FD-Score:6.34|P-value:4.47E-10 ID:SGTC_2997|Compound:9067075|FD-Score:-3.59|P-value:2.41E-4 ID:SGTC_3036|Compound:9090181|FD-Score:3.53|P-value:3.21E-4

Top fitness defect scores for YKL081W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2855 9016525 6.69 58.4 µM 4.84E-11 2 1.11E-11
2 SGTC_2992 9074591 6.34 71.4 µM 4.47E-10 2 1.18E-10
3 SGTC_1053 3366-3204 5.60 134.0 µM 3.05E-8 cell wall 12 1.07E-8
4 SGTC_2853 9015042 5.52 58.4 µM 4.75E-8 8 1.71E-8
5 SGTC_2961 9083290 5.46 32.5 µM 6.47E-8 2 2.37E-8
6 SGTC_2805 7950783 4.56 58.4 µM 5.32E-6 15 2.60E-6
7 SGTC_219 mitoxanthrone 4.52 164.4 µM 6.19E-6 DNA intercalators 39 3.06E-6
8 SGTC_883 0850-0245 4.49 24.6 µM 7.19E-6 cell wall 22 3.58E-6
9 SGTC_1056 3448-1962 4.26 115.0 µM 1.96E-5 amide catabolism 18 1.04E-5
10 SGTC_879 0831-1030 4.15 162.0 µM 3.02E-5 44 1.65E-5
11 SGTC_866 0495-0101 4.10 262.0 µM 3.77E-5 60S ribosome export 15 2.09E-5
12 SGTC_1904 st060225 4.07 78.0 µM 4.16E-5 16 2.33E-5
13 SGTC_2486 5283202 4.04 22.4 µM 4.77E-5 33 2.69E-5
14 SGTC_1110 3-hydroxy-3'-methoxyflavone 3.85 1.2 µM 9.89E-5 iron homeostasis 48 5.84E-5
15 SGTC_1227 3909-8010 3.81 46.6 µM 1.15E-4 23 6.84E-5
16 SGTC_1050 3232-1156 3.77 69.3 µM 1.36E-4 RSC complex & mRNA processing 22 8.17E-5
17 SGTC_835 0312-0010 3.70 67.2 µM 1.76E-4 13 1.08E-4
18 SGTC_1071 trifluoperazine 3.70 6.3 µM 1.76E-4 31 1.08E-4
19 SGTC_569 4130-2812 3.70 160.0 µM 1.79E-4 amide catabolism 10 1.10E-4
20 SGTC_3036 9090181 3.53 49.5 µM 3.21E-4 10 2.04E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.128 9.59E-14 YOL041C NOP12 hom Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p
0.099 8.97E-9 YKL090W CUE2 hom Protein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination
0.086 5.57E-7 YLR186W EMG1 het Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity
0.086 6.36E-7 YNR050C LYS9 hom Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112
0.086 6.43E-7 YNL232W CSL4 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
0.083 1.51E-6 YMR040W YET2 hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein
0.082 2.22E-6 YOL102C TPT1 het tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide
0.081 2.68E-6 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.080 3.21E-6 YDR085C AFR1 hom Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication
0.077 7.01E-6 YPL249C-A RPL36B hom Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication
0.077 8.44E-6 YPR140W TAZ1 hom Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; human ortholog tafazzin is implicated in Barth syndrome
0.076 1.02E-5 YPL020C ULP1 het Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions
0.076 1.18E-5 YKL202W_d YKL202W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.075 1.57E-5 YIL128W MET18 hom Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19
0.074 1.80E-5 YIR017C MET28 hom Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism