YKL084W / HOT13

Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL084W / HOT13

Click on Significant Values for Screen Details ID:SGTC_76|Compound:0433-0009|FD-Score:-4.25|P-value:1.88E-5 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:6.57|P-value:6.63E-11 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:3.90|P-value:6.76E-5 ID:SGTC_799|Compound:1310-0557|FD-Score:3.52|P-value:2.78E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:3.20|P-value:8.45E-4 ID:SGTC_889|Compound:0919-1524|FD-Score:-3.31|P-value:6.66E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:5.29|P-value:1.17E-7 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.19|P-value:8.87E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.33|P-value:5.41E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:-4.03|P-value:4.67E-5 ID:SGTC_1663|Compound:st014140|FD-Score:3.42|P-value:4.02E-4 ID:SGTC_1751|Compound:st044839|FD-Score:-3.52|P-value:3.17E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.32|P-value:5.69E-4 ID:SGTC_1888|Compound:azatryptophan|FD-Score:3.27|P-value:6.65E-4 ID:SGTC_1923|Compound:st058458|FD-Score:3.60|P-value:2.09E-4 ID:SGTC_1930|Compound:st074634|FD-Score:3.76|P-value:1.15E-4 ID:SGTC_1967|Compound:st070177|FD-Score:3.42|P-value:3.99E-4 ID:SGTC_2045|Compound:5106652|FD-Score:-3.56|P-value:2.77E-4 ID:SGTC_2185|Compound:6113692|FD-Score:3.82|P-value:9.04E-5 ID:SGTC_2343|Compound:7990716|FD-Score:3.79|P-value:1.02E-4 ID:SGTC_2348|Compound:9022543|FD-Score:4.97|P-value:5.71E-7 ID:SGTC_2400|Compound:5203494|FD-Score:6.01|P-value:2.13E-9 ID:SGTC_2433|Compound:5607874|FD-Score:-3.50|P-value:3.52E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:3.71|P-value:1.41E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:-3.31|P-value:6.78E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:-3.45|P-value:4.14E-4 ID:SGTC_2773|Compound:thioridazine|FD-Score:3.96|P-value:5.20E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:4.70|P-value:2.14E-6 ID:SGTC_2939|Compound:9044578|FD-Score:3.25|P-value:7.22E-4 ID:SGTC_3205|Compound:9113483|FD-Score:3.45|P-value:3.60E-4 ID:SGTC_76|Compound:0433-0009|FD-Score:-4.25|P-value:1.88E-5 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:6.57|P-value:6.63E-11 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:3.90|P-value:6.76E-5 ID:SGTC_799|Compound:1310-0557|FD-Score:3.52|P-value:2.78E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:3.20|P-value:8.45E-4 ID:SGTC_889|Compound:0919-1524|FD-Score:-3.31|P-value:6.66E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:5.29|P-value:1.17E-7 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.19|P-value:8.87E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.33|P-value:5.41E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:-4.03|P-value:4.67E-5 ID:SGTC_1663|Compound:st014140|FD-Score:3.42|P-value:4.02E-4 ID:SGTC_1751|Compound:st044839|FD-Score:-3.52|P-value:3.17E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.32|P-value:5.69E-4 ID:SGTC_1888|Compound:azatryptophan|FD-Score:3.27|P-value:6.65E-4 ID:SGTC_1923|Compound:st058458|FD-Score:3.60|P-value:2.09E-4 ID:SGTC_1930|Compound:st074634|FD-Score:3.76|P-value:1.15E-4 ID:SGTC_1967|Compound:st070177|FD-Score:3.42|P-value:3.99E-4 ID:SGTC_2045|Compound:5106652|FD-Score:-3.56|P-value:2.77E-4 ID:SGTC_2185|Compound:6113692|FD-Score:3.82|P-value:9.04E-5 ID:SGTC_2343|Compound:7990716|FD-Score:3.79|P-value:1.02E-4 ID:SGTC_2348|Compound:9022543|FD-Score:4.97|P-value:5.71E-7 ID:SGTC_2400|Compound:5203494|FD-Score:6.01|P-value:2.13E-9 ID:SGTC_2433|Compound:5607874|FD-Score:-3.50|P-value:3.52E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:3.71|P-value:1.41E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:-3.31|P-value:6.78E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:-3.45|P-value:4.14E-4 ID:SGTC_2773|Compound:thioridazine|FD-Score:3.96|P-value:5.20E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:4.70|P-value:2.14E-6 ID:SGTC_2939|Compound:9044578|FD-Score:3.25|P-value:7.22E-4 ID:SGTC_3205|Compound:9113483|FD-Score:3.45|P-value:3.60E-4

Top fitness defect scores for YKL084W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_219 mitoxanthrone 6.57 164.4 µM 6.63E-11 DNA intercalators 18 2.49E-11
2 SGTC_2400 5203494 6.01 178.7 µM 2.13E-9 RSC complex & mRNA processing 7 9.47E-10
3 SGTC_943 3253-1379 5.29 105.0 µM 1.17E-7 9 6.26E-8
4 SGTC_2348 9022543 4.97 33.4 µM 5.71E-7 mitochondrial processes 13 3.30E-7
5 SGTC_2777 climbazole 4.70 1.5 µM 2.14E-6 iron homeostasis 24 1.32E-6
6 SGTC_2773 thioridazine 3.96 9.1 µM 5.20E-5 9 3.71E-5
7 SGTC_239 5hr heat shock (37°C) + mitomycin C 3.90 819.4 µM 6.76E-5 heat shock/prefoldin 37 4.87E-5
8 SGTC_2185 6113692 3.82 200.0 µM 9.04E-5 ergosterol biosynthesis 20 6.61E-5
9 SGTC_2343 7990716 3.79 14.8 µM 1.02E-4 23 7.52E-5
10 SGTC_1930 st074634 3.76 34.3 µM 1.15E-4 RNA pol III & RNase P/MRP 41 8.49E-5
11 SGTC_2552 juarezic acid 3.71 87.0 µM 1.41E-4 TSC3-RPN4 49 1.05E-4
12 SGTC_1923 st058458 3.60 19.1 µM 2.09E-4 45 1.58E-4
13 SGTC_799 1310-0557 3.52 151.0 µM 2.78E-4 superoxide 39 2.14E-4
14 SGTC_3205 9113483 3.45 49.5 µM 3.60E-4 50 2.79E-4
15 SGTC_1967 st070177 3.42 51.4 µM 3.99E-4 29 3.11E-4
16 SGTC_1663 st014140 3.42 55.4 µM 4.02E-4 60S ribosome export 29 3.14E-4
17 SGTC_1357 1503-1303 3.33 39.5 µM 5.41E-4 66 4.28E-4
18 SGTC_1785 bathophenanthroline 3.32 24.0 µM 5.69E-4 heme requiring 80 4.52E-4
19 SGTC_1888 azatryptophan 3.27 97.5 µM 6.65E-4 39 5.31E-4
20 SGTC_2939 9044578 3.25 71.4 µM 7.22E-4 66 5.78E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.199 2.86E-31 YDL140C RPO21 het RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
0.120 2.85E-12 YDR406W PDR15 hom Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element
0.119 4.50E-12 YDR164C SEC1 het Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function
0.107 5.69E-10 YMR224C MRE11 hom Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress
0.104 1.44E-9 YPL262W FUM1 hom Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
0.099 8.04E-9 YNL250W RAD50 hom Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress
0.097 1.81E-8 YKL198C PTK1 hom Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein
0.097 1.98E-8 YGR005C TFG2 het TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30
0.094 5.60E-8 YFR032C_p RRT5_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation
0.093 6.12E-8 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.092 8.50E-8 YFR032C-A RPL29 hom Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog
0.091 1.11E-7 YGL128C CWC23 het Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p
0.089 2.11E-7 YEL011W GLC3 hom Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.089 2.48E-7 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.089 2.64E-7 YMR044W IOC4 hom Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing