YKL085W / MDH1

Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL085W / MDH1

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:3.98|P-value:4.82E-5 ID:SGTC_86|Compound:0417-1630|FD-Score:3.30|P-value:6.20E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.28|P-value:6.55E-4 ID:SGTC_222|Compound:nsc-310342|FD-Score:3.36|P-value:4.98E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:3.37|P-value:4.84E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-3.25|P-value:6.99E-4 ID:SGTC_891|Compound:0929-0073|FD-Score:-3.19|P-value:8.58E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:5.16|P-value:2.12E-7 ID:SGTC_938|Compound:3126-1755|FD-Score:-3.54|P-value:2.51E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:3.74|P-value:1.27E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:3.62|P-value:1.94E-4 ID:SGTC_984|Compound:1492-0255|FD-Score:3.62|P-value:1.97E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:-3.55|P-value:2.49E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.34|P-value:1.05E-5 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.26|P-value:7.12E-4 ID:SGTC_1711|Compound:st032263|FD-Score:4.57|P-value:3.81E-6 ID:SGTC_1747|Compound:st037496|FD-Score:4.32|P-value:1.15E-5 ID:SGTC_1755|Compound:st041960|FD-Score:-3.19|P-value:8.65E-4 ID:SGTC_2052|Compound:5265182|FD-Score:-3.40|P-value:4.22E-4 ID:SGTC_2067|Compound:5224629|FD-Score:3.36|P-value:4.96E-4 ID:SGTC_2223|Compound:6903751|FD-Score:3.25|P-value:7.32E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-3.88|P-value:7.11E-5 ID:SGTC_2415|Compound:st077333|FD-Score:-3.29|P-value:6.16E-4 ID:SGTC_2416|Compound:tofa|FD-Score:4.04|P-value:3.73E-5 ID:SGTC_2465|Compound:5809242|FD-Score:3.83|P-value:8.67E-5 ID:SGTC_2474|Compound:5321570|FD-Score:-3.38|P-value:4.49E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:-6.10|P-value:1.10E-9 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-5.00|P-value:4.73E-7 ID:SGTC_2961|Compound:9083290|FD-Score:4.87|P-value:9.07E-7 ID:SGTC_3019|Compound:9081971|FD-Score:3.21|P-value:8.40E-4 ID:SGTC_3206|Compound:9114350|FD-Score:3.54|P-value:2.62E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:3.98|P-value:4.82E-5 ID:SGTC_86|Compound:0417-1630|FD-Score:3.30|P-value:6.20E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.28|P-value:6.55E-4 ID:SGTC_222|Compound:nsc-310342|FD-Score:3.36|P-value:4.98E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:3.37|P-value:4.84E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-3.25|P-value:6.99E-4 ID:SGTC_891|Compound:0929-0073|FD-Score:-3.19|P-value:8.58E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:5.16|P-value:2.12E-7 ID:SGTC_938|Compound:3126-1755|FD-Score:-3.54|P-value:2.51E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:3.74|P-value:1.27E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:3.62|P-value:1.94E-4 ID:SGTC_984|Compound:1492-0255|FD-Score:3.62|P-value:1.97E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:-3.55|P-value:2.49E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.34|P-value:1.05E-5 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.26|P-value:7.12E-4 ID:SGTC_1711|Compound:st032263|FD-Score:4.57|P-value:3.81E-6 ID:SGTC_1747|Compound:st037496|FD-Score:4.32|P-value:1.15E-5 ID:SGTC_1755|Compound:st041960|FD-Score:-3.19|P-value:8.65E-4 ID:SGTC_2052|Compound:5265182|FD-Score:-3.40|P-value:4.22E-4 ID:SGTC_2067|Compound:5224629|FD-Score:3.36|P-value:4.96E-4 ID:SGTC_2223|Compound:6903751|FD-Score:3.25|P-value:7.32E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-3.88|P-value:7.11E-5 ID:SGTC_2415|Compound:st077333|FD-Score:-3.29|P-value:6.16E-4 ID:SGTC_2416|Compound:tofa|FD-Score:4.04|P-value:3.73E-5 ID:SGTC_2465|Compound:5809242|FD-Score:3.83|P-value:8.67E-5 ID:SGTC_2474|Compound:5321570|FD-Score:-3.38|P-value:4.49E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:-6.10|P-value:1.10E-9 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-5.00|P-value:4.73E-7 ID:SGTC_2961|Compound:9083290|FD-Score:4.87|P-value:9.07E-7 ID:SGTC_3019|Compound:9081971|FD-Score:3.21|P-value:8.40E-4 ID:SGTC_3206|Compound:9114350|FD-Score:3.54|P-value:2.62E-4

Top fitness defect scores for YKL085W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_930 2810-3379 5.16 265.0 µM 2.12E-7 2 1.22E-7
2 SGTC_2961 9083290 4.87 32.5 µM 9.07E-7 8 5.50E-7
3 SGTC_1711 st032263 4.57 62.8 µM 3.81E-6 9 2.45E-6
4 SGTC_1133 3473-0247 4.34 50.0 µM 1.05E-5 RSC complex & mRNA processing 35 7.01E-6
5 SGTC_1747 st037496 4.32 66.6 µM 1.15E-5 10 7.74E-6
6 SGTC_2416 tofa 4.04 880.0 nM 3.73E-5 sphingolipid biosynthesis & PDR1 46 2.62E-5
7 SGTC_36 0335-0881 3.98 14.2 µM 4.82E-5 ubiquinone biosynthesis & proteasome 118 3.42E-5
8 SGTC_2465 5809242 3.83 33.2 µM 8.67E-5 3 6.30E-5
9 SGTC_972 1319-0219 3.74 195.0 µM 1.27E-4 37 9.32E-5
10 SGTC_974 1326-0144 3.62 138.0 µM 1.94E-4 ERG2 54 1.46E-4
11 SGTC_984 1492-0255 3.62 2.9 µM 1.97E-4 SWF1 & branched chain AA biosynthesis 37 1.48E-4
12 SGTC_3206 9114350 3.54 49.5 µM 2.62E-4 29 1.99E-4
13 SGTC_359 0081-0058 3.37 15.9 µM 4.84E-4 60S ribosome export 73 3.76E-4
14 SGTC_2067 5224629 3.36 34.1 µM 4.96E-4 42 3.86E-4
15 SGTC_222 nsc-310342 3.36 10.7 µM 4.98E-4 7 3.87E-4
16 SGTC_86 0417-1630 3.30 471.9 µM 6.20E-4 ubiquinone biosynthesis & proteasome 196 4.86E-4
17 SGTC_158 k038-0087 3.28 26.4 µM 6.55E-4 ubiquinone biosynthesis & proteasome 189 5.15E-4
18 SGTC_1151 3909-8316 3.26 15.3 µM 7.12E-4 44 5.61E-4
19 SGTC_2223 6903751 3.25 107.6 µM 7.32E-4 superoxide 23 5.78E-4
20 SGTC_3019 9081971 3.21 49.5 µM 8.40E-4 34 6.66E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.196 1.80E-30 YKR028W SAP190 hom Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
0.195 4.89E-30 YGR255C COQ6 hom Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
0.193 2.32E-29 YML110C COQ5 hom 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes
0.184 7.63E-27 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.156 1.29E-19 YNR041C COQ2 hom Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis
0.148 6.35E-18 YML034W SRC1 hom Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance
0.144 5.85E-17 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.143 1.03E-16 YDR204W COQ4 hom Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.141 2.59E-16 YMR119W-A_d YMR119W-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.139 5.75E-16 YER041W YEN1 hom Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p
0.139 7.22E-16 YML034C-A_d YML034C-A_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.138 1.01E-15 YPR064W_d YPR064W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.137 1.68E-15 YJL072C PSF2 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.136 3.24E-15 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.136 3.58E-15 YLR202C_d YLR202C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron