HIPHOP chemogenomics database

YKL087C / CYT2

Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1

Zygosity: Homozygous strain

fixedexpanded
Profile for YKL087C / CYT2

Click on Significant Values for Screen Details

Top fitness defect scores for YKL087C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_236	1509-0011	24.50	5.3 µM	4.84E-133		34	2.45E-133
2	SGTC_1635	st007035	16.50	42.4 µM	1.88E-61		30	1.38E-61
3	SGTC_3141	9094609	9.50	49.5 µM	1.16E-21	iron homeostasis	1	1.04E-21
4	SGTC_219	mitoxanthrone	8.39	164.4 µM	2.66E-17	DNA intercalators	12	2.45E-17
5	SGTC_3142	9086498	5.33	49.5 µM	5.05E-8	iron homeostasis	14	4.88E-8
6	SGTC_1364	1653-0028	5.14	37.6 µM	1.42E-7		6	1.38E-7
7	SGTC_2936	9038235	4.88	15.6 µM	5.40E-7		7	5.25E-7
8	SGTC_2757	clomiphene	4.26	4.2 µM	1.04E-5	fatty acid desaturase (OLE1)	29	1.02E-5
9	SGTC_1194	1488-0194	4.24	225.0 µM	1.17E-5		23	1.14E-5
10	SGTC_2103	5378138	4.23	10.0 µM	1.21E-5		9	1.18E-5
11	SGTC_2336	9001819	4.14	198.7 µM	1.74E-5	heme biosynthesis & mitochondrial translocase	47	1.71E-5
12	SGTC_1748	st037455	4.10	53.6 µM	2.07E-5		28	2.03E-5
13	SGTC_1750	st081859	4.07	2.4 µM	2.37E-5	endomembrane recycling	176	2.32E-5
14	SGTC_513	4245-1575	4.04	41.7 µM	2.68E-5	unfolded protein response	110	2.63E-5
15	SGTC_2117	5521686	3.94	200.0 µM	4.09E-5		11	4.01E-5
16	SGTC_2612	dictamnine	3.92	15.5 µM	4.51E-5	RNA pol III & RNase P/MRP	43	4.43E-5
17	SGTC_3281	9140613	3.61	49.5 µM	1.55E-4	RPP1 & pyrimidine depletion	31	1.53E-4
18	SGTC_2223	6903751	3.59	107.6 µM	1.67E-4	superoxide	17	1.65E-4
19	SGTC_15	0266-0086	3.56	27.1 µM	1.87E-4	endomembrane recycling	78	1.84E-4
20	SGTC_3222	9128301	3.52	49.5 µM	2.18E-4	60S ribosome export	37	2.15E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.609	0	YJL166W	QCR8	hom	Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.606	0	YJR120W	YJR120W	hom	Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.603	0	YHR050W-A_p	YHR050W-A_p	hom	Protein of unknown function; identified by expression profiling and mass spectrometry
0.576	1.69E-295	YGL237C	HAP2	hom	Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.554	4.21E-269	YDR375C	BCS1	hom	Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.547	5.62E-261	YDR230W_d	YDR230W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.532	8.33E-245	YPL215W	CBP3	hom	Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.526	2.50E-238	YPL013C	MRPS16	hom	Mitochondrial ribosomal protein of the small subunit
0.526	7.55E-238	YMR035W	IMP2	hom	Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.523	4.48E-235	YEL059C-A	SOM1	hom	Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.522	1.15E-233	YMR151W_d	YIM2_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.518	7.39E-230	YGR183C	QCR9	hom	Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
0.512	1.27E-223	YOR330C	MIP1	hom	Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.504	2.83E-215	YOR065W	CYT1	hom	Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
0.495	2.68E-207	YPL132W	COX11	hom	Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p