YKL087C / CYT2

Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL087C / CYT2

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:3.56|P-value:1.87E-4 ID:SGTC_47|Compound:0149-0135|FD-Score:3.36|P-value:3.89E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:8.39|P-value:2.66E-17 ID:SGTC_222|Compound:nsc-310342|FD-Score:3.38|P-value:3.72E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:24.50|P-value:4.84E-133 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.30|P-value:4.90E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:-3.30|P-value:4.81E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:4.04|P-value:2.68E-5 ID:SGTC_632|Compound:0781-2353|FD-Score:-3.55|P-value:1.96E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:3.50|P-value:2.33E-4 ID:SGTC_775|Compound:4577-0580|FD-Score:-3.31|P-value:4.64E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:-3.17|P-value:7.84E-4 ID:SGTC_1194|Compound:1488-0194|FD-Score:4.24|P-value:1.17E-5 ID:SGTC_1354|Compound:1498-1681|FD-Score:3.13|P-value:8.88E-4 ID:SGTC_1364|Compound:1653-0028|FD-Score:5.14|P-value:1.42E-7 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.28|P-value:5.23E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.18|P-value:7.42E-4 ID:SGTC_1635|Compound:st007035|FD-Score:16.50|P-value:1.88E-61 ID:SGTC_1748|Compound:st037455|FD-Score:4.10|P-value:2.07E-5 ID:SGTC_1750|Compound:st081859|FD-Score:4.07|P-value:2.37E-5 ID:SGTC_2069|Compound:5228469|FD-Score:3.51|P-value:2.28E-4 ID:SGTC_2103|Compound:5378138|FD-Score:4.23|P-value:1.21E-5 ID:SGTC_2117|Compound:5521686|FD-Score:3.94|P-value:4.09E-5 ID:SGTC_2223|Compound:6903751|FD-Score:3.59|P-value:1.67E-4 ID:SGTC_2336|Compound:9001819|FD-Score:4.14|P-value:1.74E-5 ID:SGTC_2338|Compound:7999974|FD-Score:-3.37|P-value:3.74E-4 ID:SGTC_2511|Compound:berberine|FD-Score:-3.13|P-value:8.86E-4 ID:SGTC_2521|Compound:angolensin|FD-Score:3.21|P-value:6.78E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:3.35|P-value:4.14E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.92|P-value:4.51E-5 ID:SGTC_2757|Compound:clomiphene|FD-Score:4.26|P-value:1.04E-5 ID:SGTC_2823|Compound:7987624|FD-Score:-4.70|P-value:1.30E-6 ID:SGTC_2826|Compound:7992541|FD-Score:-4.23|P-value:1.19E-5 ID:SGTC_2911|Compound:7942208|FD-Score:3.31|P-value:4.70E-4 ID:SGTC_2933|Compound:9026794|FD-Score:3.28|P-value:5.27E-4 ID:SGTC_2936|Compound:9038235|FD-Score:4.88|P-value:5.40E-7 ID:SGTC_2943|Compound:9068529|FD-Score:3.17|P-value:7.66E-4 ID:SGTC_3141|Compound:9094609|FD-Score:9.50|P-value:1.16E-21 ID:SGTC_3142|Compound:9086498|FD-Score:5.33|P-value:5.05E-8 ID:SGTC_3144|Compound:9087057|FD-Score:-6.57|P-value:2.66E-11 ID:SGTC_3160|Compound:9100216|FD-Score:-4.65|P-value:1.74E-6 ID:SGTC_3165|Compound:9101544|FD-Score:-3.81|P-value:6.97E-5 ID:SGTC_3187|Compound:9109394|FD-Score:-3.18|P-value:7.49E-4 ID:SGTC_3222|Compound:9128301|FD-Score:3.52|P-value:2.18E-4 ID:SGTC_3267|Compound:9137951|FD-Score:-4.06|P-value:2.51E-5 ID:SGTC_3281|Compound:9140613|FD-Score:3.61|P-value:1.55E-4 ID:SGTC_3343|Compound:9148379|FD-Score:3.26|P-value:5.67E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:3.56|P-value:1.87E-4 ID:SGTC_47|Compound:0149-0135|FD-Score:3.36|P-value:3.89E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:8.39|P-value:2.66E-17 ID:SGTC_222|Compound:nsc-310342|FD-Score:3.38|P-value:3.72E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:24.50|P-value:4.84E-133 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.30|P-value:4.90E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:-3.30|P-value:4.81E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:4.04|P-value:2.68E-5 ID:SGTC_632|Compound:0781-2353|FD-Score:-3.55|P-value:1.96E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:3.50|P-value:2.33E-4 ID:SGTC_775|Compound:4577-0580|FD-Score:-3.31|P-value:4.64E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:-3.17|P-value:7.84E-4 ID:SGTC_1194|Compound:1488-0194|FD-Score:4.24|P-value:1.17E-5 ID:SGTC_1354|Compound:1498-1681|FD-Score:3.13|P-value:8.88E-4 ID:SGTC_1364|Compound:1653-0028|FD-Score:5.14|P-value:1.42E-7 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.28|P-value:5.23E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.18|P-value:7.42E-4 ID:SGTC_1635|Compound:st007035|FD-Score:16.50|P-value:1.88E-61 ID:SGTC_1748|Compound:st037455|FD-Score:4.10|P-value:2.07E-5 ID:SGTC_1750|Compound:st081859|FD-Score:4.07|P-value:2.37E-5 ID:SGTC_2069|Compound:5228469|FD-Score:3.51|P-value:2.28E-4 ID:SGTC_2103|Compound:5378138|FD-Score:4.23|P-value:1.21E-5 ID:SGTC_2117|Compound:5521686|FD-Score:3.94|P-value:4.09E-5 ID:SGTC_2223|Compound:6903751|FD-Score:3.59|P-value:1.67E-4 ID:SGTC_2336|Compound:9001819|FD-Score:4.14|P-value:1.74E-5 ID:SGTC_2338|Compound:7999974|FD-Score:-3.37|P-value:3.74E-4 ID:SGTC_2511|Compound:berberine|FD-Score:-3.13|P-value:8.86E-4 ID:SGTC_2521|Compound:angolensin|FD-Score:3.21|P-value:6.78E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:3.35|P-value:4.14E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.92|P-value:4.51E-5 ID:SGTC_2757|Compound:clomiphene|FD-Score:4.26|P-value:1.04E-5 ID:SGTC_2823|Compound:7987624|FD-Score:-4.70|P-value:1.30E-6 ID:SGTC_2826|Compound:7992541|FD-Score:-4.23|P-value:1.19E-5 ID:SGTC_2911|Compound:7942208|FD-Score:3.31|P-value:4.70E-4 ID:SGTC_2933|Compound:9026794|FD-Score:3.28|P-value:5.27E-4 ID:SGTC_2936|Compound:9038235|FD-Score:4.88|P-value:5.40E-7 ID:SGTC_2943|Compound:9068529|FD-Score:3.17|P-value:7.66E-4 ID:SGTC_3141|Compound:9094609|FD-Score:9.50|P-value:1.16E-21 ID:SGTC_3142|Compound:9086498|FD-Score:5.33|P-value:5.05E-8 ID:SGTC_3144|Compound:9087057|FD-Score:-6.57|P-value:2.66E-11 ID:SGTC_3160|Compound:9100216|FD-Score:-4.65|P-value:1.74E-6 ID:SGTC_3165|Compound:9101544|FD-Score:-3.81|P-value:6.97E-5 ID:SGTC_3187|Compound:9109394|FD-Score:-3.18|P-value:7.49E-4 ID:SGTC_3222|Compound:9128301|FD-Score:3.52|P-value:2.18E-4 ID:SGTC_3267|Compound:9137951|FD-Score:-4.06|P-value:2.51E-5 ID:SGTC_3281|Compound:9140613|FD-Score:3.61|P-value:1.55E-4 ID:SGTC_3343|Compound:9148379|FD-Score:3.26|P-value:5.67E-4

Top fitness defect scores for YKL087C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 24.50 5.3 µM 4.84E-133 34 2.45E-133
2 SGTC_1635 st007035 16.50 42.4 µM 1.88E-61 30 1.38E-61
3 SGTC_3141 9094609 9.50 49.5 µM 1.16E-21 iron homeostasis 1 1.04E-21
4 SGTC_219 mitoxanthrone 8.39 164.4 µM 2.66E-17 DNA intercalators 12 2.45E-17
5 SGTC_3142 9086498 5.33 49.5 µM 5.05E-8 iron homeostasis 14 4.88E-8
6 SGTC_1364 1653-0028 5.14 37.6 µM 1.42E-7 6 1.38E-7
7 SGTC_2936 9038235 4.88 15.6 µM 5.40E-7 7 5.25E-7
8 SGTC_2757 clomiphene 4.26 4.2 µM 1.04E-5 fatty acid desaturase (OLE1) 29 1.02E-5
9 SGTC_1194 1488-0194 4.24 225.0 µM 1.17E-5 23 1.14E-5
10 SGTC_2103 5378138 4.23 10.0 µM 1.21E-5 9 1.18E-5
11 SGTC_2336 9001819 4.14 198.7 µM 1.74E-5 heme biosynthesis & mitochondrial translocase 47 1.71E-5
12 SGTC_1748 st037455 4.10 53.6 µM 2.07E-5 28 2.03E-5
13 SGTC_1750 st081859 4.07 2.4 µM 2.37E-5 endomembrane recycling 176 2.32E-5
14 SGTC_513 4245-1575 4.04 41.7 µM 2.68E-5 unfolded protein response 110 2.63E-5
15 SGTC_2117 5521686 3.94 200.0 µM 4.09E-5 11 4.01E-5
16 SGTC_2612 dictamnine 3.92 15.5 µM 4.51E-5 RNA pol III & RNase P/MRP 43 4.43E-5
17 SGTC_3281 9140613 3.61 49.5 µM 1.55E-4 RPP1 & pyrimidine depletion 31 1.53E-4
18 SGTC_2223 6903751 3.59 107.6 µM 1.67E-4 superoxide 17 1.65E-4
19 SGTC_15 0266-0086 3.56 27.1 µM 1.87E-4 endomembrane recycling 78 1.84E-4
20 SGTC_3222 9128301 3.52 49.5 µM 2.18E-4 60S ribosome export 37 2.15E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.609 0 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.606 0 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.603 0 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.576 1.69E-295 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.554 4.21E-269 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.547 5.62E-261 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.532 8.33E-245 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.526 2.50E-238 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.526 7.55E-238 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.523 4.48E-235 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.522 1.15E-233 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.518 7.39E-230 YGR183C QCR9 hom Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
0.512 1.27E-223 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.504 2.83E-215 YOR065W CYT1 hom Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
0.495 2.68E-207 YPL132W COX11 hom Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p