YKL089W / MIF2

Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL089W / MIF2

Click on Significant Values for Screen Details ID:SGTC_86|Compound:0417-1630|FD-Score:4.38|P-value:2.08E-6 ID:SGTC_157|Compound:1348-1695|FD-Score:4.04|P-value:1.10E-5 ID:SGTC_192|Compound:3455-0609|FD-Score:-3.41|P-value:2.42E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:6.84|P-value:4.18E-13 ID:SGTC_393|Compound:acivicin|FD-Score:-3.46|P-value:1.97E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:3.28|P-value:2.70E-4 ID:SGTC_505|Compound:gw-5074|FD-Score:2.94|P-value:9.51E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:3.20|P-value:3.72E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-3.18|P-value:5.84E-4 ID:SGTC_981|Compound:1488-1094|FD-Score:-3.42|P-value:2.29E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.73|P-value:6.71E-5 ID:SGTC_1212|Compound:0520-0165|FD-Score:4.68|P-value:4.50E-7 ID:SGTC_1245|Compound:0418-0076|FD-Score:2.98|P-value:8.31E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.17|P-value:4.18E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:5.01|P-value:7.49E-8 ID:SGTC_1313|Compound:1220-0047|FD-Score:-3.65|P-value:9.45E-5 ID:SGTC_1357|Compound:1503-1303|FD-Score:-3.53|P-value:1.49E-4 ID:SGTC_1361|Compound:1608-0041|FD-Score:-3.67|P-value:8.49E-5 ID:SGTC_1413|Compound:3910-0535|FD-Score:2.94|P-value:9.47E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.21|P-value:3.56E-4 ID:SGTC_1634|Compound:st006576|FD-Score:3.72|P-value:4.52E-5 ID:SGTC_1779|Compound:st039427|FD-Score:3.32|P-value:2.36E-4 ID:SGTC_2112|Compound:5488546|FD-Score:3.55|P-value:9.45E-5 ID:SGTC_2285|Compound:7945142|FD-Score:2.99|P-value:8.10E-4 ID:SGTC_2411|Compound:st077133|FD-Score:3.29|P-value:2.64E-4 ID:SGTC_2416|Compound:tofa|FD-Score:-6.10|P-value:1.67E-10 ID:SGTC_2417|Compound:cerulenin|FD-Score:-3.22|P-value:4.88E-4 ID:SGTC_2455|Compound:5377712|FD-Score:-3.75|P-value:6.05E-5 ID:SGTC_2467|Compound:5474540|FD-Score:-3.49|P-value:1.80E-4 ID:SGTC_2481|Compound:5478948|FD-Score:-3.17|P-value:6.06E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.63|P-value:1.01E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:3.26|P-value:3.01E-4 ID:SGTC_2585|Compound:alliin|FD-Score:5.28|P-value:1.53E-8 ID:SGTC_2950|Compound:9072895|FD-Score:-3.42|P-value:2.31E-4 ID:SGTC_3064|Compound:1327-0027|FD-Score:3.13|P-value:4.80E-4 ID:SGTC_3141|Compound:9094609|FD-Score:4.21|P-value:4.80E-6 ID:SGTC_3143|Compound:9086658|FD-Score:3.99|P-value:1.37E-5 ID:SGTC_3147|Compound:9097027|FD-Score:4.38|P-value:2.14E-6 ID:SGTC_3210|Compound:9128157|FD-Score:-3.83|P-value:4.25E-5 ID:SGTC_86|Compound:0417-1630|FD-Score:4.38|P-value:2.08E-6 ID:SGTC_157|Compound:1348-1695|FD-Score:4.04|P-value:1.10E-5 ID:SGTC_192|Compound:3455-0609|FD-Score:-3.41|P-value:2.42E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:6.84|P-value:4.18E-13 ID:SGTC_393|Compound:acivicin|FD-Score:-3.46|P-value:1.97E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:3.28|P-value:2.70E-4 ID:SGTC_505|Compound:gw-5074|FD-Score:2.94|P-value:9.51E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:3.20|P-value:3.72E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-3.18|P-value:5.84E-4 ID:SGTC_981|Compound:1488-1094|FD-Score:-3.42|P-value:2.29E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.73|P-value:6.71E-5 ID:SGTC_1212|Compound:0520-0165|FD-Score:4.68|P-value:4.50E-7 ID:SGTC_1245|Compound:0418-0076|FD-Score:2.98|P-value:8.31E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.17|P-value:4.18E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:5.01|P-value:7.49E-8 ID:SGTC_1313|Compound:1220-0047|FD-Score:-3.65|P-value:9.45E-5 ID:SGTC_1357|Compound:1503-1303|FD-Score:-3.53|P-value:1.49E-4 ID:SGTC_1361|Compound:1608-0041|FD-Score:-3.67|P-value:8.49E-5 ID:SGTC_1413|Compound:3910-0535|FD-Score:2.94|P-value:9.47E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.21|P-value:3.56E-4 ID:SGTC_1634|Compound:st006576|FD-Score:3.72|P-value:4.52E-5 ID:SGTC_1779|Compound:st039427|FD-Score:3.32|P-value:2.36E-4 ID:SGTC_2112|Compound:5488546|FD-Score:3.55|P-value:9.45E-5 ID:SGTC_2285|Compound:7945142|FD-Score:2.99|P-value:8.10E-4 ID:SGTC_2411|Compound:st077133|FD-Score:3.29|P-value:2.64E-4 ID:SGTC_2416|Compound:tofa|FD-Score:-6.10|P-value:1.67E-10 ID:SGTC_2417|Compound:cerulenin|FD-Score:-3.22|P-value:4.88E-4 ID:SGTC_2455|Compound:5377712|FD-Score:-3.75|P-value:6.05E-5 ID:SGTC_2467|Compound:5474540|FD-Score:-3.49|P-value:1.80E-4 ID:SGTC_2481|Compound:5478948|FD-Score:-3.17|P-value:6.06E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.63|P-value:1.01E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:3.26|P-value:3.01E-4 ID:SGTC_2585|Compound:alliin|FD-Score:5.28|P-value:1.53E-8 ID:SGTC_2950|Compound:9072895|FD-Score:-3.42|P-value:2.31E-4 ID:SGTC_3064|Compound:1327-0027|FD-Score:3.13|P-value:4.80E-4 ID:SGTC_3141|Compound:9094609|FD-Score:4.21|P-value:4.80E-6 ID:SGTC_3143|Compound:9086658|FD-Score:3.99|P-value:1.37E-5 ID:SGTC_3147|Compound:9097027|FD-Score:4.38|P-value:2.14E-6 ID:SGTC_3210|Compound:9128157|FD-Score:-3.83|P-value:4.25E-5

Top fitness defect scores for YKL089W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_373 0844-0104 6.84 25.9 µM 4.18E-13 ERAD & cell cycle 2 3.89E-12 0.69
2 SGTC_2585 alliin 5.28 100.0 µM 1.53E-8 4 6.30E-8 0.37
3 SGTC_1298 1079-1278 5.01 263.0 µM 7.49E-8 DNA damage response 1 2.71E-7 0.58
4 SGTC_1212 0520-0165 4.68 585.0 µM 4.50E-7 4 1.42E-6 0.30
5 SGTC_86 0417-1630 4.38 471.9 µM 2.08E-6 ubiquinone biosynthesis & proteasome 23 5.80E-6 0.08
6 SGTC_3147 9097027 4.38 49.5 µM 2.14E-6 4 5.97E-6 0.33
7 SGTC_3141 9094609 4.21 49.5 µM 4.80E-6 iron homeostasis 12 1.25E-5 0.13
8 SGTC_157 1348-1695 4.04 65.8 µM 1.10E-5 iron homeostasis 32 2.70E-5 0.09
9 SGTC_3143 9086658 3.99 49.5 µM 1.37E-5 14 3.30E-5 0.05
10 SGTC_1634 st006576 3.72 41.0 µM 4.52E-5 TSC3-RPN4 9 9.90E-5 0.01
11 SGTC_2112 5488546 3.55 200.0 µM 9.45E-5 TSC3-RPN4 11 1.95E-4 0.06
12 SGTC_1779 st039427 3.32 55.0 µM 2.36E-4 5 4.53E-4 0.02
13 SGTC_2411 st077133 3.29 39.9 µM 2.64E-4 RNA pol III & RNase P/MRP 27 5.02E-4 0.02
14 SGTC_439 amiodarone 3.28 34.4 µM 2.70E-4 NEO1-PIK1 14 5.12E-4 0.08
15 SGTC_2544 sphondin 3.26 68.5 µM 3.01E-4 RNA pol III & RNase P/MRP 48 5.66E-4 0.04
16 SGTC_1528 arabinosylhypoxanthine 3.21 74.6 µM 3.56E-4 15 6.61E-4 2.51E-4
17 SGTC_533 1222-0222 3.20 6.7 µM 3.72E-4 PDR1 17 6.88E-4 0.05
18 SGTC_1258 0671-0043 3.17 57.1 µM 4.18E-4 11 7.66E-4 0.06
19 SGTC_3064 1327-0027 3.13 10.0 µM 4.80E-4 10 8.70E-4 0.16
20 SGTC_2285 7945142 2.99 200.0 µM 8.10E-4 22 0.00141 1.24E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.128 1.19E-13 YGL114W_p YGL114W_p hom Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters
0.125 4.09E-13 YGR123C PPT1 hom Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth
0.118 7.07E-12 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.116 1.74E-11 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.110 1.91E-10 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.108 3.26E-10 YDR242W AMD2 hom Putative amidase
0.107 5.83E-10 YLR202C_d YLR202C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron
0.105 1.15E-9 YOR271C_p FSF1_p hom Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis
0.099 8.04E-9 YDL110C TMA17 hom Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.097 1.81E-8 YER019C-A SBH2 hom Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p
0.097 2.15E-8 YCL012C YCL012C hom Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene
0.093 6.13E-8 YPL082C MOT1 het Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
0.092 9.68E-8 YKR018C YKR018C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication
0.092 1.05E-7 YJR040W GEF1 hom Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
0.091 1.24E-7 YER172C BRR2 het RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD