YKL095W / YJU2

Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL095W / YJU2

Click on Significant Values for Screen Details ID:SGTC_83|Compound:0170-0301|FD-Score:-3.15|P-value:4.74E-4 ID:SGTC_92|Compound:0063-0024|FD-Score:3.60|P-value:8.52E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:-5.29|P-value:1.49E-8 ID:SGTC_168|Compound:3970-0862|FD-Score:3.58|P-value:9.29E-5 ID:SGTC_306|Compound:1672-3436|FD-Score:-3.07|P-value:6.36E-4 ID:SGTC_491|Compound:tetrandrine|FD-Score:-3.69|P-value:5.53E-5 ID:SGTC_579|Compound:4491-1009|FD-Score:3.49|P-value:1.34E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:-3.58|P-value:8.52E-5 ID:SGTC_828|Compound:2074-0507|FD-Score:2.99|P-value:9.02E-4 ID:SGTC_896|Compound:0964-0007|FD-Score:-3.06|P-value:6.68E-4 ID:SGTC_918|Compound:glabranin|FD-Score:3.47|P-value:1.49E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:-3.55|P-value:9.77E-5 ID:SGTC_1001|Compound:4048-4355|FD-Score:3.29|P-value:2.92E-4 ID:SGTC_1078|Compound:idebenone|FD-Score:3.02|P-value:8.26E-4 ID:SGTC_1109|Compound:tnp00067|FD-Score:-3.43|P-value:1.63E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:3.21|P-value:4.03E-4 ID:SGTC_1143|Compound:3454-2408|FD-Score:3.15|P-value:5.03E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:3.45|P-value:1.57E-4 ID:SGTC_1324|Compound:1418-0026|FD-Score:3.08|P-value:6.51E-4 ID:SGTC_1419|Compound:3993-2473|FD-Score:4.72|P-value:4.02E-7 ID:SGTC_1428|Compound:4049-0204|FD-Score:-4.05|P-value:1.09E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.41|P-value:1.83E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:-3.20|P-value:3.88E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:3.15|P-value:5.00E-4 ID:SGTC_1602|Compound:st001878|FD-Score:3.44|P-value:1.65E-4 ID:SGTC_1720|Compound:st037298|FD-Score:4.92|P-value:1.37E-7 ID:SGTC_1721|Compound:st082152|FD-Score:6.62|P-value:2.20E-12 ID:SGTC_1727|Compound:st034309|FD-Score:-3.52|P-value:1.13E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:3.68|P-value:6.02E-5 ID:SGTC_1876|Compound:myriocin|FD-Score:3.39|P-value:2.02E-4 ID:SGTC_1899|Compound:st063254|FD-Score:3.05|P-value:7.41E-4 ID:SGTC_1977|Compound:st070720|FD-Score:3.69|P-value:5.93E-5 ID:SGTC_2164|Compound:5685496|FD-Score:4.45|P-value:1.64E-6 ID:SGTC_2172|Compound:5754775|FD-Score:3.31|P-value:2.72E-4 ID:SGTC_2286|Compound:7945697|FD-Score:3.27|P-value:3.17E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:4.07|P-value:1.08E-5 ID:SGTC_3072|Compound:9116419|FD-Score:3.23|P-value:3.78E-4 ID:SGTC_3077|Compound:9117319|FD-Score:-4.14|P-value:7.10E-6 ID:SGTC_3083|Compound:9117465|FD-Score:-3.31|P-value:2.57E-4 ID:SGTC_3089|Compound:9117425|FD-Score:-3.16|P-value:4.59E-4 ID:SGTC_3333|Compound:9141820|FD-Score:3.47|P-value:1.46E-4 ID:SGTC_83|Compound:0170-0301|FD-Score:-3.15|P-value:4.74E-4 ID:SGTC_92|Compound:0063-0024|FD-Score:3.60|P-value:8.52E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:-5.29|P-value:1.49E-8 ID:SGTC_168|Compound:3970-0862|FD-Score:3.58|P-value:9.29E-5 ID:SGTC_306|Compound:1672-3436|FD-Score:-3.07|P-value:6.36E-4 ID:SGTC_491|Compound:tetrandrine|FD-Score:-3.69|P-value:5.53E-5 ID:SGTC_579|Compound:4491-1009|FD-Score:3.49|P-value:1.34E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:-3.58|P-value:8.52E-5 ID:SGTC_828|Compound:2074-0507|FD-Score:2.99|P-value:9.02E-4 ID:SGTC_896|Compound:0964-0007|FD-Score:-3.06|P-value:6.68E-4 ID:SGTC_918|Compound:glabranin|FD-Score:3.47|P-value:1.49E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:-3.55|P-value:9.77E-5 ID:SGTC_1001|Compound:4048-4355|FD-Score:3.29|P-value:2.92E-4 ID:SGTC_1078|Compound:idebenone|FD-Score:3.02|P-value:8.26E-4 ID:SGTC_1109|Compound:tnp00067|FD-Score:-3.43|P-value:1.63E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:3.21|P-value:4.03E-4 ID:SGTC_1143|Compound:3454-2408|FD-Score:3.15|P-value:5.03E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:3.45|P-value:1.57E-4 ID:SGTC_1324|Compound:1418-0026|FD-Score:3.08|P-value:6.51E-4 ID:SGTC_1419|Compound:3993-2473|FD-Score:4.72|P-value:4.02E-7 ID:SGTC_1428|Compound:4049-0204|FD-Score:-4.05|P-value:1.09E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.41|P-value:1.83E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:-3.20|P-value:3.88E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:3.15|P-value:5.00E-4 ID:SGTC_1602|Compound:st001878|FD-Score:3.44|P-value:1.65E-4 ID:SGTC_1720|Compound:st037298|FD-Score:4.92|P-value:1.37E-7 ID:SGTC_1721|Compound:st082152|FD-Score:6.62|P-value:2.20E-12 ID:SGTC_1727|Compound:st034309|FD-Score:-3.52|P-value:1.13E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:3.68|P-value:6.02E-5 ID:SGTC_1876|Compound:myriocin|FD-Score:3.39|P-value:2.02E-4 ID:SGTC_1899|Compound:st063254|FD-Score:3.05|P-value:7.41E-4 ID:SGTC_1977|Compound:st070720|FD-Score:3.69|P-value:5.93E-5 ID:SGTC_2164|Compound:5685496|FD-Score:4.45|P-value:1.64E-6 ID:SGTC_2172|Compound:5754775|FD-Score:3.31|P-value:2.72E-4 ID:SGTC_2286|Compound:7945697|FD-Score:3.27|P-value:3.17E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:4.07|P-value:1.08E-5 ID:SGTC_3072|Compound:9116419|FD-Score:3.23|P-value:3.78E-4 ID:SGTC_3077|Compound:9117319|FD-Score:-4.14|P-value:7.10E-6 ID:SGTC_3083|Compound:9117465|FD-Score:-3.31|P-value:2.57E-4 ID:SGTC_3089|Compound:9117425|FD-Score:-3.16|P-value:4.59E-4 ID:SGTC_3333|Compound:9141820|FD-Score:3.47|P-value:1.46E-4

Top fitness defect scores for YKL095W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1721 st082152 6.62 9.3 µM 2.20E-12 60S ribosome export 2 1.74E-11 0.12
2 SGTC_1720 st037298 4.92 9.5 µM 1.37E-7 3 4.33E-7 0.07
3 SGTC_1419 3993-2473 4.72 62.6 µM 4.02E-7 1 1.16E-6 0.35
4 SGTC_2164 5685496 4.45 200.0 µM 1.64E-6 6 4.20E-6 0.33
5 SGTC_2588 indole-3-carbinol 4.07 100.0 µM 1.08E-5 6 2.38E-5 0.03
6 SGTC_1977 st070720 3.69 65.3 µM 5.93E-5 3 1.14E-4 0.82
7 SGTC_1869 3',7'-dimethoxyflavone 3.68 40.3 µM 6.02E-5 3 1.15E-4 0.09
8 SGTC_92 0063-0024 3.60 660.9 µM 8.52E-5 2 1.59E-4 0.17
9 SGTC_168 3970-0862 3.58 530.5 µM 9.29E-5 57 1.72E-4 0.06
10 SGTC_579 4491-1009 3.49 110.0 µM 1.34E-4 60S ribosome export 11 2.40E-4 0.10
11 SGTC_3333 9141820 3.47 12.1 µM 1.46E-4 10 2.60E-4 0.10
12 SGTC_918 glabranin 3.47 770.7 nM 1.49E-4 12 2.65E-4 0.18
13 SGTC_1269 0831-0796 3.45 3.0 µM 1.57E-4 60S ribosome export 10 2.79E-4 0.07
14 SGTC_1602 st001878 3.44 87.6 µM 1.65E-4 12 2.92E-4 0.00
15 SGTC_1495 4469-0029 3.41 237.0 µM 1.83E-4 9 3.20E-4 0.18
16 SGTC_1876 myriocin 3.39 605.0 nM 2.02E-4 12 3.52E-4 0.04
17 SGTC_2172 5754775 3.31 163.9 µM 2.72E-4 16 4.61E-4 0.03
18 SGTC_1001 4048-4355 3.29 44.4 µM 2.92E-4 copper-dependent oxidative stress 19 4.94E-4 0.24
19 SGTC_2286 7945697 3.27 125.0 µM 3.17E-4 20 5.32E-4 0.37
20 SGTC_3072 9116419 3.23 49.5 µM 3.78E-4 ERG2 10 6.25E-4 0.07

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.144 5.34E-17 YGR030C POP6 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.136 2.72E-15 YGR212W SLI1 hom N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
0.103 1.98E-9 YOR190W SPR1 hom Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance
0.102 2.72E-9 YDR071C PAA1 hom Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication
0.102 2.89E-9 YHL041W_d YHL041W_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
0.102 3.32E-9 YBL004W UTP20 het Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
0.102 3.54E-9 YBR125C PTC4 hom Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity
0.101 4.55E-9 YLR195C NMT1 het N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
0.099 1.05E-8 YPL033C SRL4 hom Protein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p
0.098 1.28E-8 YBR155W CNS1 het TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
0.096 2.75E-8 YDL153C SAS10 het Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance
0.095 3.13E-8 YDR235W PRP42 het U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats
0.093 5.99E-8 YKL152C GPM1 het Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis
0.093 6.82E-8 YER043C SAH1 het S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor
0.092 1.07E-7 YMR296C LCB1 het Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine