YKL104C / GFA1

Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL104C / GFA1

Click on Significant Values for Screen Details ID:SGTC_146|Compound:3448-8650|FD-Score:3.45|P-value:7.85E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:5.31|P-value:4.86E-7 ID:SGTC_254|Compound:nsc-64875|FD-Score:5.49|P-value:2.12E-7 ID:SGTC_255|Compound:tunicamycin|FD-Score:18.60|P-value:4.02E-67 ID:SGTC_260|Compound:nsc-65238|FD-Score:5.31|P-value:4.88E-7 ID:SGTC_363|Compound:0455-0021|FD-Score:16.90|P-value:7.34E-56 ID:SGTC_467|Compound:ro 31-8220|FD-Score:6.37|P-value:2.01E-9 ID:SGTC_492|Compound:tmb-8|FD-Score:11.90|P-value:1.59E-28 ID:SGTC_655|Compound:k060-0012|FD-Score:3.73|P-value:3.05E-4 ID:SGTC_677|Compound:0929-0063|FD-Score:4.65|P-value:9.18E-6 ID:SGTC_690|Compound:glyoxal|FD-Score:16.60|P-value:1.23E-53 ID:SGTC_765|Compound:1319-0117|FD-Score:3.44|P-value:8.01E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.69|P-value:3.51E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:-3.39|P-value:6.90E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:4.25|P-value:4.57E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:-3.31|P-value:9.17E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:-3.31|P-value:9.10E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:3.93|P-value:1.55E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:8.02|P-value:6.06E-14 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.54|P-value:5.85E-4 ID:SGTC_1664|Compound:st014217|FD-Score:3.57|P-value:5.27E-4 ID:SGTC_1727|Compound:st034309|FD-Score:-3.61|P-value:3.41E-4 ID:SGTC_1737|Compound:st035557|FD-Score:4.61|P-value:1.10E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:4.63|P-value:1.01E-5 ID:SGTC_1902|Compound:st060212|FD-Score:-3.43|P-value:6.15E-4 ID:SGTC_1983|Compound:st071813|FD-Score:4.18|P-value:5.97E-5 ID:SGTC_2016|Compound:5121758|FD-Score:4.01|P-value:1.14E-4 ID:SGTC_2229|Compound:7354935|FD-Score:3.94|P-value:1.49E-4 ID:SGTC_2413|Compound:inermin|FD-Score:3.47|P-value:7.34E-4 ID:SGTC_2458|Compound:5808907|FD-Score:-3.99|P-value:8.79E-5 ID:SGTC_2532|Compound:xanthone|FD-Score:-3.53|P-value:4.45E-4 ID:SGTC_2813|Compound:7971799|FD-Score:4.23|P-value:4.95E-5 ID:SGTC_2993|Compound:9054257|FD-Score:3.42|P-value:8.67E-4 ID:SGTC_3159|Compound:9100096|FD-Score:3.56|P-value:5.37E-4 ID:SGTC_3347|Compound:9188721|FD-Score:21.20|P-value:2.20E-86 ID:SGTC_146|Compound:3448-8650|FD-Score:3.45|P-value:7.85E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:5.31|P-value:4.86E-7 ID:SGTC_254|Compound:nsc-64875|FD-Score:5.49|P-value:2.12E-7 ID:SGTC_255|Compound:tunicamycin|FD-Score:18.60|P-value:4.02E-67 ID:SGTC_260|Compound:nsc-65238|FD-Score:5.31|P-value:4.88E-7 ID:SGTC_363|Compound:0455-0021|FD-Score:16.90|P-value:7.34E-56 ID:SGTC_467|Compound:ro 31-8220|FD-Score:6.37|P-value:2.01E-9 ID:SGTC_492|Compound:tmb-8|FD-Score:11.90|P-value:1.59E-28 ID:SGTC_655|Compound:k060-0012|FD-Score:3.73|P-value:3.05E-4 ID:SGTC_677|Compound:0929-0063|FD-Score:4.65|P-value:9.18E-6 ID:SGTC_690|Compound:glyoxal|FD-Score:16.60|P-value:1.23E-53 ID:SGTC_765|Compound:1319-0117|FD-Score:3.44|P-value:8.01E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.69|P-value:3.51E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:-3.39|P-value:6.90E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:4.25|P-value:4.57E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:-3.31|P-value:9.17E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:-3.31|P-value:9.10E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:3.93|P-value:1.55E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:8.02|P-value:6.06E-14 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.54|P-value:5.85E-4 ID:SGTC_1664|Compound:st014217|FD-Score:3.57|P-value:5.27E-4 ID:SGTC_1727|Compound:st034309|FD-Score:-3.61|P-value:3.41E-4 ID:SGTC_1737|Compound:st035557|FD-Score:4.61|P-value:1.10E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:4.63|P-value:1.01E-5 ID:SGTC_1902|Compound:st060212|FD-Score:-3.43|P-value:6.15E-4 ID:SGTC_1983|Compound:st071813|FD-Score:4.18|P-value:5.97E-5 ID:SGTC_2016|Compound:5121758|FD-Score:4.01|P-value:1.14E-4 ID:SGTC_2229|Compound:7354935|FD-Score:3.94|P-value:1.49E-4 ID:SGTC_2413|Compound:inermin|FD-Score:3.47|P-value:7.34E-4 ID:SGTC_2458|Compound:5808907|FD-Score:-3.99|P-value:8.79E-5 ID:SGTC_2532|Compound:xanthone|FD-Score:-3.53|P-value:4.45E-4 ID:SGTC_2813|Compound:7971799|FD-Score:4.23|P-value:4.95E-5 ID:SGTC_2993|Compound:9054257|FD-Score:3.42|P-value:8.67E-4 ID:SGTC_3159|Compound:9100096|FD-Score:3.56|P-value:5.37E-4 ID:SGTC_3347|Compound:9188721|FD-Score:21.20|P-value:2.20E-86

Top fitness defect scores for YKL104C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3347 9188721 21.20 44.7 µM 2.20E-86 1 4.98E-100 5.76
2 SGTC_255 tunicamycin 18.60 200.5 nM 4.02E-67 unfolded protein response 3 1.07E-77 9.43
3 SGTC_363 0455-0021 16.90 3.1 µM 7.34E-56 azole & statin 1 1.22E-64 3.70
4 SGTC_690 glyoxal 16.60 20.3 µM 1.23E-53 2 4.62E-62 4.87
5 SGTC_492 tmb-8 11.90 126.0 µM 1.59E-28 1 6.18E-33 6.96
6 SGTC_1130 3454-2858 8.02 15.9 µM 6.06E-14 NEO1 3 5.17E-16 1.70
7 SGTC_467 ro 31-8220 6.37 72.8 µM 2.01E-9 calcium & mitochondrial duress 6 9.15E-11 0.29
8 SGTC_254 nsc-64875 5.49 3.8 µM 2.12E-7 ergosterol depletion effects on membrane 2 2.05E-8 1.31
9 SGTC_249 wiskostatin 5.31 11.2 µM 4.86E-7 2 5.36E-8 1.68
10 SGTC_260 nsc-65238 5.31 4.0 µM 4.88E-7 endomembrane recycling 1 5.38E-8 0.62
11 SGTC_677 0929-0063 4.65 136.0 µM 9.18E-6 1 1.63E-6 1.12
12 SGTC_1789 tunicamycin 4.63 25.0 nM 1.01E-5 unfolded protein response 9 1.81E-6 0.08
13 SGTC_1737 st035557 4.61 69.7 µM 1.10E-5 2 2.01E-6 0.08
14 SGTC_924 2046-0044 4.25 21.3 µM 4.57E-5 5 1.05E-5 0.02
15 SGTC_2813 7971799 4.23 71.4 µM 4.95E-5 5 1.15E-5 0.28
16 SGTC_1983 st071813 4.18 46.9 µM 5.97E-5 RPP1 & pyrimidine depletion 5 1.43E-5 0.07
17 SGTC_2016 5121758 4.01 140.0 µM 1.14E-4 redox potentiating 14 3.04E-5 0.11
18 SGTC_2229 7354935 3.94 190.0 µM 1.49E-4 RSC complex & mRNA processing 17 4.13E-5 0.17
19 SGTC_999 4022-0066 3.93 22.7 µM 1.55E-4 Golgi 12 4.32E-5 0.02
20 SGTC_655 k060-0012 3.73 42.0 µM 3.05E-4 9 9.47E-5 0.17

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.427 2.36E-148 YGR190C_d YGR190C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
0.354 2.35E-99 YJR139C HOM6 hom Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
0.331 2.84E-86 YBR243C ALG7 het UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.331 2.98E-86 YGR191W HIP1 het High-affinity histidine permease, also involved in the transport of manganese ions
0.209 1.43E-34 YNL307C MCK1 hom Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family
0.193 1.78E-29 YNR031C SSK2 hom MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress
0.175 1.93E-24 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.174 4.33E-24 YGR170W PSD2 hom Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine
0.172 1.30E-23 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.157 5.05E-20 YOR364W_d YOR364W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C
0.156 8.69E-20 YNL287W SEC21 het Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
0.143 8.12E-17 YKL213C DOA1 hom WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
0.140 3.82E-16 YLR113W HOG1 hom Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress
0.138 1.12E-15 YKR034W DAL80 hom Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication
0.137 1.36E-15 YDL133W SRF1 hom Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients