YKL113C / RAD27

5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL113C / RAD27

Click on Significant Values for Screen Details ID:SGTC_94|Compound:0334-0113|FD-Score:3.66|P-value:7.55E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:5.01|P-value:7.09E-6 ID:SGTC_260|Compound:nsc-65238|FD-Score:3.72|P-value:6.38E-4 ID:SGTC_263|Compound:alizarin|FD-Score:5.33|P-value:1.95E-6 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.95|P-value:9.04E-6 ID:SGTC_275|Compound:trichlorophene|FD-Score:7.54|P-value:3.26E-11 ID:SGTC_352|Compound:k048-0007|FD-Score:-3.79|P-value:5.15E-4 ID:SGTC_458|Compound:nppb|FD-Score:4.74|P-value:2.02E-5 ID:SGTC_574|Compound:1315-0397|FD-Score:3.67|P-value:7.46E-4 ID:SGTC_712|Compound:r015-0009|FD-Score:5.68|P-value:4.40E-7 ID:SGTC_792|Compound:4534-1957|FD-Score:3.88|P-value:3.84E-4 ID:SGTC_856|Compound:0433-0016|FD-Score:3.60|P-value:9.03E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:5.15|P-value:4.13E-6 ID:SGTC_1149|Compound:3909-7961|FD-Score:4.63|P-value:3.05E-5 ID:SGTC_1181|Compound:1222-0449|FD-Score:4.17|P-value:1.53E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:3.97|P-value:2.95E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:-6.46|P-value:1.08E-8 ID:SGTC_1375|Compound:2910-0797|FD-Score:4.93|P-value:9.71E-6 ID:SGTC_1379|Compound:2144-0755|FD-Score:5.04|P-value:6.36E-6 ID:SGTC_1381|Compound:2324-0066|FD-Score:4.93|P-value:9.90E-6 ID:SGTC_1429|Compound:4049-0205|FD-Score:4.78|P-value:1.73E-5 ID:SGTC_1434|Compound:4073-0095|FD-Score:5.28|P-value:2.37E-6 ID:SGTC_1479|Compound:0549-0158|FD-Score:4.26|P-value:1.12E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:5.53|P-value:8.33E-7 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:3.95|P-value:3.13E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:-4.65|P-value:2.86E-5 ID:SGTC_1554|Compound:st069299|FD-Score:4.24|P-value:1.18E-4 ID:SGTC_1555|Compound:citronellol|FD-Score:3.69|P-value:6.96E-4 ID:SGTC_1629|Compound:st005085|FD-Score:-3.70|P-value:6.69E-4 ID:SGTC_1655|Compound:st012546|FD-Score:4.46|P-value:5.68E-5 ID:SGTC_1656|Compound:st012921|FD-Score:3.66|P-value:7.64E-4 ID:SGTC_1834|Compound:st055981|FD-Score:4.72|P-value:2.17E-5 ID:SGTC_1843|Compound:st055809|FD-Score:4.87|P-value:1.24E-5 ID:SGTC_1921|Compound:5740136|FD-Score:-3.84|P-value:4.37E-4 ID:SGTC_1939|Compound:st074455|FD-Score:-3.87|P-value:4.02E-4 ID:SGTC_2054|Compound:5233173|FD-Score:3.88|P-value:3.87E-4 ID:SGTC_2061|Compound:5245884|FD-Score:6.55|P-value:6.83E-9 ID:SGTC_2085|Compound:5307070|FD-Score:4.43|P-value:6.20E-5 ID:SGTC_2105|Compound:5350118|FD-Score:3.90|P-value:3.63E-4 ID:SGTC_2174|Compound:5790901|FD-Score:4.05|P-value:2.22E-4 ID:SGTC_2190|Compound:6049624|FD-Score:3.67|P-value:7.34E-4 ID:SGTC_2294|Compound:7971431|FD-Score:7.79|P-value:7.50E-12 ID:SGTC_2988|Compound:9073640|FD-Score:3.69|P-value:6.94E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-4.28|P-value:1.05E-4 ID:SGTC_3189|Compound:9110682|FD-Score:3.86|P-value:4.17E-4 ID:SGTC_94|Compound:0334-0113|FD-Score:3.66|P-value:7.55E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:5.01|P-value:7.09E-6 ID:SGTC_260|Compound:nsc-65238|FD-Score:3.72|P-value:6.38E-4 ID:SGTC_263|Compound:alizarin|FD-Score:5.33|P-value:1.95E-6 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.95|P-value:9.04E-6 ID:SGTC_275|Compound:trichlorophene|FD-Score:7.54|P-value:3.26E-11 ID:SGTC_352|Compound:k048-0007|FD-Score:-3.79|P-value:5.15E-4 ID:SGTC_458|Compound:nppb|FD-Score:4.74|P-value:2.02E-5 ID:SGTC_574|Compound:1315-0397|FD-Score:3.67|P-value:7.46E-4 ID:SGTC_712|Compound:r015-0009|FD-Score:5.68|P-value:4.40E-7 ID:SGTC_792|Compound:4534-1957|FD-Score:3.88|P-value:3.84E-4 ID:SGTC_856|Compound:0433-0016|FD-Score:3.60|P-value:9.03E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:5.15|P-value:4.13E-6 ID:SGTC_1149|Compound:3909-7961|FD-Score:4.63|P-value:3.05E-5 ID:SGTC_1181|Compound:1222-0449|FD-Score:4.17|P-value:1.53E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:3.97|P-value:2.95E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:-6.46|P-value:1.08E-8 ID:SGTC_1375|Compound:2910-0797|FD-Score:4.93|P-value:9.71E-6 ID:SGTC_1379|Compound:2144-0755|FD-Score:5.04|P-value:6.36E-6 ID:SGTC_1381|Compound:2324-0066|FD-Score:4.93|P-value:9.90E-6 ID:SGTC_1429|Compound:4049-0205|FD-Score:4.78|P-value:1.73E-5 ID:SGTC_1434|Compound:4073-0095|FD-Score:5.28|P-value:2.37E-6 ID:SGTC_1479|Compound:0549-0158|FD-Score:4.26|P-value:1.12E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:5.53|P-value:8.33E-7 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:3.95|P-value:3.13E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:-4.65|P-value:2.86E-5 ID:SGTC_1554|Compound:st069299|FD-Score:4.24|P-value:1.18E-4 ID:SGTC_1555|Compound:citronellol|FD-Score:3.69|P-value:6.96E-4 ID:SGTC_1629|Compound:st005085|FD-Score:-3.70|P-value:6.69E-4 ID:SGTC_1655|Compound:st012546|FD-Score:4.46|P-value:5.68E-5 ID:SGTC_1656|Compound:st012921|FD-Score:3.66|P-value:7.64E-4 ID:SGTC_1834|Compound:st055981|FD-Score:4.72|P-value:2.17E-5 ID:SGTC_1843|Compound:st055809|FD-Score:4.87|P-value:1.24E-5 ID:SGTC_1921|Compound:5740136|FD-Score:-3.84|P-value:4.37E-4 ID:SGTC_1939|Compound:st074455|FD-Score:-3.87|P-value:4.02E-4 ID:SGTC_2054|Compound:5233173|FD-Score:3.88|P-value:3.87E-4 ID:SGTC_2061|Compound:5245884|FD-Score:6.55|P-value:6.83E-9 ID:SGTC_2085|Compound:5307070|FD-Score:4.43|P-value:6.20E-5 ID:SGTC_2105|Compound:5350118|FD-Score:3.90|P-value:3.63E-4 ID:SGTC_2174|Compound:5790901|FD-Score:4.05|P-value:2.22E-4 ID:SGTC_2190|Compound:6049624|FD-Score:3.67|P-value:7.34E-4 ID:SGTC_2294|Compound:7971431|FD-Score:7.79|P-value:7.50E-12 ID:SGTC_2988|Compound:9073640|FD-Score:3.69|P-value:6.94E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-4.28|P-value:1.05E-4 ID:SGTC_3189|Compound:9110682|FD-Score:3.86|P-value:4.17E-4

Top fitness defect scores for YKL113C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2294 7971431 7.79 200.0 µM 7.50E-12 1 3.35E-15
2 SGTC_275 trichlorophene 7.54 5.7 µM 3.26E-11 mitochondrial stress 85 2.36E-14
3 SGTC_2061 5245884 6.55 81.0 µM 6.83E-9 tubulin folding & SWR complex 4 2.79E-11
4 SGTC_712 r015-0009 5.68 80.6 µM 4.40E-7 11 6.88E-9
5 SGTC_1507 4075-3230 5.53 155.0 µM 8.33E-7 4 1.60E-8
6 SGTC_263 alizarin 5.33 107.9 µM 1.95E-6 iron homeostasis 30 4.94E-8
7 SGTC_1434 4073-0095 5.28 163.0 µM 2.37E-6 3 6.35E-8
8 SGTC_1048 3161-0300 5.15 24.5 µM 4.13E-6 6 1.33E-7
9 SGTC_1379 2144-0755 5.04 36.5 µM 6.36E-6 10 2.34E-7
10 SGTC_177 1327-0068 5.01 8.4 µM 7.09E-6 26 2.70E-7
11 SGTC_273 hydroxyurea 4.95 18.1 mM 9.04E-6 heme biosynthesis & mitochondrial translocase 16 3.72E-7
12 SGTC_1375 2910-0797 4.93 186.0 µM 9.71E-6 13 4.09E-7
13 SGTC_1381 2324-0066 4.93 62.1 µM 9.90E-6 14 4.20E-7
14 SGTC_1843 st055809 4.87 45.5 µM 1.24E-5 6 5.65E-7
15 SGTC_1429 4049-0205 4.78 9.5 µM 1.73E-5 2 8.78E-7
16 SGTC_458 nppb 4.74 133.0 µM 2.02E-5 6 1.07E-6
17 SGTC_1834 st055981 4.72 52.8 µM 2.17E-5 RPP1 & pyrimidine depletion 14 1.18E-6
18 SGTC_1149 3909-7961 4.63 23.3 µM 3.05E-5 5 1.85E-6
19 SGTC_1655 st012546 4.46 55.1 µM 5.68E-5 20 4.19E-6
20 SGTC_2085 5307070 4.43 144.1 µM 6.20E-5 superoxide 19 4.69E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.099 8.63E-9 YLR060W FRS1 het Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
0.095 3.01E-8 YJL027C_p YJL027C_p hom Putative protein of unknown function
0.095 3.71E-8 YDR115W YDR115W hom Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress
0.088 2.87E-7 YOL098C_p YOL098C_p hom Putative metalloprotease
0.087 3.92E-7 YGL071W AFT1 hom Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.086 6.71E-7 YFL036W RPO41 hom Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.085 8.97E-7 YMR058W FET3 hom Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress
0.078 5.68E-6 YOR309C_d YOR309C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
0.073 2.47E-5 YBL031W SHE1 hom Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function
0.072 3.11E-5 YJL092W SRS2 hom DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.072 3.21E-5 YLR184W_d YLR184W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.066 1.32E-4 YIL093C RSM25 hom Mitochondrial ribosomal protein of the small subunit
0.065 1.67E-4 YDL128W VCX1 hom Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter
0.064 2.08E-4 YKR076W ECM4 hom Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.064 2.09E-4 YMR138W CIN4 hom GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog