HIPHOP chemogenomics database

YKL113C / RAD27

5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family

Zygosity: Homozygous strain

fixedexpanded
Profile for YKL113C / RAD27

Click on Significant Values for Screen Details

Top fitness defect scores for YKL113C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2294	7971431	7.79	200.0 µM	7.50E-12		1	3.35E-15
2	SGTC_275	trichlorophene	7.54	5.7 µM	3.26E-11	mitochondrial stress	85	2.36E-14
3	SGTC_2061	5245884	6.55	81.0 µM	6.83E-9	tubulin folding & SWR complex	4	2.79E-11
4	SGTC_712	r015-0009	5.68	80.6 µM	4.40E-7		11	6.88E-9
5	SGTC_1507	4075-3230	5.53	155.0 µM	8.33E-7		4	1.60E-8
6	SGTC_263	alizarin	5.33	107.9 µM	1.95E-6	iron homeostasis	30	4.94E-8
7	SGTC_1434	4073-0095	5.28	163.0 µM	2.37E-6		3	6.35E-8
8	SGTC_1048	3161-0300	5.15	24.5 µM	4.13E-6		6	1.33E-7
9	SGTC_1379	2144-0755	5.04	36.5 µM	6.36E-6		10	2.34E-7
10	SGTC_177	1327-0068	5.01	8.4 µM	7.09E-6		26	2.70E-7
11	SGTC_273	hydroxyurea	4.95	18.1 mM	9.04E-6	heme biosynthesis & mitochondrial translocase	16	3.72E-7
12	SGTC_1375	2910-0797	4.93	186.0 µM	9.71E-6		13	4.09E-7
13	SGTC_1381	2324-0066	4.93	62.1 µM	9.90E-6		14	4.20E-7
14	SGTC_1843	st055809	4.87	45.5 µM	1.24E-5		6	5.65E-7
15	SGTC_1429	4049-0205	4.78	9.5 µM	1.73E-5		2	8.78E-7
16	SGTC_458	nppb	4.74	133.0 µM	2.02E-5		6	1.07E-6
17	SGTC_1834	st055981	4.72	52.8 µM	2.17E-5	RPP1 & pyrimidine depletion	14	1.18E-6
18	SGTC_1149	3909-7961	4.63	23.3 µM	3.05E-5		5	1.85E-6
19	SGTC_1655	st012546	4.46	55.1 µM	5.68E-5		20	4.19E-6
20	SGTC_2085	5307070	4.43	144.1 µM	6.20E-5	superoxide	19	4.69E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.099	8.63E-9	YLR060W	FRS1	het	Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
0.095	3.01E-8	YJL027C_p	YJL027C_p	hom	Putative protein of unknown function
0.095	3.71E-8	YDR115W	YDR115W	hom	Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress
0.088	2.87E-7	YOL098C_p	YOL098C_p	hom	Putative metalloprotease
0.087	3.92E-7	YGL071W	AFT1	hom	Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.086	6.71E-7	YFL036W	RPO41	hom	Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.085	8.97E-7	YMR058W	FET3	hom	Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress
0.078	5.68E-6	YOR309C_d	YOR309C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
0.073	2.47E-5	YBL031W	SHE1	hom	Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function
0.072	3.11E-5	YJL092W	SRS2	hom	DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.072	3.21E-5	YLR184W_d	YLR184W_d	hom	Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.066	1.32E-4	YIL093C	RSM25	hom	Mitochondrial ribosomal protein of the small subunit
0.065	1.67E-4	YDL128W	VCX1	hom	Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter
0.064	2.08E-4	YKR076W	ECM4	hom	Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.064	2.09E-4	YMR138W	CIN4	hom	GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog