5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family
Zygosity: Homozygous strain
fixedexpanded
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Top fitness defect scores for YKL113C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.099 | 8.63E-9 | YLR060W | FRS1 | het | Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control |
0.095 | 3.01E-8 | YJL027C_p | YJL027C_p | hom | Putative protein of unknown function |
0.095 | 3.71E-8 | YDR115W | YDR115W | hom | Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress |
0.088 | 2.87E-7 | YOL098C_p | YOL098C_p | hom | Putative metalloprotease |
0.087 | 3.92E-7 | YGL071W | AFT1 | hom | Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress |
0.086 | 6.71E-7 | YFL036W | RPO41 | hom | Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation |
0.085 | 8.97E-7 | YMR058W | FET3 | hom | Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress |
0.078 | 5.68E-6 | YOR309C_d | YOR309C_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 |
0.073 | 2.47E-5 | YBL031W | SHE1 | hom | Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function |
0.072 | 3.11E-5 | YJL092W | SRS2 | hom | DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability |
0.072 | 3.21E-5 | YLR184W_d | YLR184W_d | hom | Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data |
0.066 | 1.32E-4 | YIL093C | RSM25 | hom | Mitochondrial ribosomal protein of the small subunit |
0.065 | 1.67E-4 | YDL128W | VCX1 | hom | Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter |
0.064 | 2.08E-4 | YKR076W | ECM4 | hom | Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm |
0.064 | 2.09E-4 | YMR138W | CIN4 | hom | GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog |