YKL116C / PRR1

Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL116C / PRR1

Click on Significant Values for Screen Details ID:SGTC_107|Compound:0358-0022|FD-Score:-2.84|P-value:7.76E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:3.68|P-value:1.20E-5 ID:SGTC_131|Compound:0141-0277|FD-Score:4.70|P-value:3.73E-8 ID:SGTC_364|Compound:0083-0097|FD-Score:2.78|P-value:6.65E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:2.74|P-value:7.81E-4 ID:SGTC_393|Compound:acivicin|FD-Score:-2.90|P-value:6.18E-4 ID:SGTC_462|Compound:ag-879|FD-Score:5.91|P-value:7.17E-12 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:3.72|P-value:9.39E-6 ID:SGTC_489|Compound:paxilline|FD-Score:-3.16|P-value:2.13E-4 ID:SGTC_490|Compound:phenamil|FD-Score:-3.72|P-value:1.64E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:8.30|P-value:1.52E-21 ID:SGTC_516|Compound:3992-2719|FD-Score:-3.47|P-value:5.33E-5 ID:SGTC_556|Compound:4058-0063|FD-Score:-3.96|P-value:4.73E-6 ID:SGTC_741|Compound:4582-0002|FD-Score:-3.03|P-value:3.75E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.64|P-value:2.40E-5 ID:SGTC_865|Compound:0488-0499|FD-Score:4.07|P-value:1.47E-6 ID:SGTC_875|Compound:0709-0210|FD-Score:2.85|P-value:5.03E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:2.74|P-value:7.63E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.39|P-value:4.85E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.72|P-value:3.24E-8 ID:SGTC_1100|Compound:amiodarone|FD-Score:2.72|P-value:8.53E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.52|P-value:2.07E-7 ID:SGTC_1143|Compound:3454-2408|FD-Score:3.31|P-value:7.06E-5 ID:SGTC_1146|Compound:3579-1023|FD-Score:3.54|P-value:2.32E-5 ID:SGTC_1153|Compound:4055-0057|FD-Score:3.88|P-value:4.17E-6 ID:SGTC_1206|Compound:3978-0017|FD-Score:3.38|P-value:5.00E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.70|P-value:1.06E-5 ID:SGTC_1284|Compound:0929-0043|FD-Score:2.80|P-value:6.03E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.07|P-value:3.10E-4 ID:SGTC_1746|Compound:st038117|FD-Score:-3.10|P-value:2.77E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-4.46|P-value:2.87E-7 ID:SGTC_1907|Compound:st060210|FD-Score:2.75|P-value:7.39E-4 ID:SGTC_1943|Compound:st076331|FD-Score:-3.19|P-value:1.88E-4 ID:SGTC_1947|Compound:st076332|FD-Score:-5.35|P-value:9.61E-10 ID:SGTC_2023|Compound:5144124|FD-Score:-3.06|P-value:3.24E-4 ID:SGTC_2125|Compound:5246583|FD-Score:2.82|P-value:5.65E-4 ID:SGTC_2255|Compound:7941087|FD-Score:2.81|P-value:5.79E-4 ID:SGTC_2259|Compound:7964009|FD-Score:2.89|P-value:4.23E-4 ID:SGTC_2305|Compound:7443522|FD-Score:2.73|P-value:8.08E-4 ID:SGTC_2352|Compound:9027995|FD-Score:-3.58|P-value:3.19E-5 ID:SGTC_2428|Compound:5615643|FD-Score:3.19|P-value:1.18E-4 ID:SGTC_2429|Compound:5902844|FD-Score:2.92|P-value:3.83E-4 ID:SGTC_2436|Compound:5633522|FD-Score:3.73|P-value:9.16E-6 ID:SGTC_2569|Compound:tetrandrine|FD-Score:2.70|P-value:9.18E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:4.50|P-value:1.27E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:-2.78|P-value:9.80E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:2.80|P-value:6.08E-4 ID:SGTC_2623|Compound:humulene|FD-Score:2.82|P-value:5.69E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-4.70|P-value:7.07E-8 ID:SGTC_2630|Compound:antimycin a|FD-Score:-4.56|P-value:1.65E-7 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:10.20|P-value:3.09E-31 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:4.49|P-value:1.30E-7 ID:SGTC_2867|Compound:9039098|FD-Score:-3.20|P-value:1.77E-4 ID:SGTC_2980|Compound:9014342|FD-Score:3.04|P-value:2.27E-4 ID:SGTC_3207|Compound:9114163|FD-Score:3.05|P-value:2.18E-4 ID:SGTC_3212|Compound:9128307|FD-Score:5.31|P-value:5.99E-10 ID:SGTC_3313|Compound:9133968|FD-Score:2.80|P-value:6.12E-4 ID:SGTC_3314|Compound:9135860|FD-Score:3.81|P-value:6.08E-6 ID:SGTC_3329|Compound:9141233|FD-Score:2.90|P-value:4.11E-4 ID:SGTC_3333|Compound:9141820|FD-Score:-3.77|P-value:1.28E-5 ID:SGTC_107|Compound:0358-0022|FD-Score:-2.84|P-value:7.76E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:3.68|P-value:1.20E-5 ID:SGTC_131|Compound:0141-0277|FD-Score:4.70|P-value:3.73E-8 ID:SGTC_364|Compound:0083-0097|FD-Score:2.78|P-value:6.65E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:2.74|P-value:7.81E-4 ID:SGTC_393|Compound:acivicin|FD-Score:-2.90|P-value:6.18E-4 ID:SGTC_462|Compound:ag-879|FD-Score:5.91|P-value:7.17E-12 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:3.72|P-value:9.39E-6 ID:SGTC_489|Compound:paxilline|FD-Score:-3.16|P-value:2.13E-4 ID:SGTC_490|Compound:phenamil|FD-Score:-3.72|P-value:1.64E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:8.30|P-value:1.52E-21 ID:SGTC_516|Compound:3992-2719|FD-Score:-3.47|P-value:5.33E-5 ID:SGTC_556|Compound:4058-0063|FD-Score:-3.96|P-value:4.73E-6 ID:SGTC_741|Compound:4582-0002|FD-Score:-3.03|P-value:3.75E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.64|P-value:2.40E-5 ID:SGTC_865|Compound:0488-0499|FD-Score:4.07|P-value:1.47E-6 ID:SGTC_875|Compound:0709-0210|FD-Score:2.85|P-value:5.03E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:2.74|P-value:7.63E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.39|P-value:4.85E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.72|P-value:3.24E-8 ID:SGTC_1100|Compound:amiodarone|FD-Score:2.72|P-value:8.53E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.52|P-value:2.07E-7 ID:SGTC_1143|Compound:3454-2408|FD-Score:3.31|P-value:7.06E-5 ID:SGTC_1146|Compound:3579-1023|FD-Score:3.54|P-value:2.32E-5 ID:SGTC_1153|Compound:4055-0057|FD-Score:3.88|P-value:4.17E-6 ID:SGTC_1206|Compound:3978-0017|FD-Score:3.38|P-value:5.00E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.70|P-value:1.06E-5 ID:SGTC_1284|Compound:0929-0043|FD-Score:2.80|P-value:6.03E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.07|P-value:3.10E-4 ID:SGTC_1746|Compound:st038117|FD-Score:-3.10|P-value:2.77E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-4.46|P-value:2.87E-7 ID:SGTC_1907|Compound:st060210|FD-Score:2.75|P-value:7.39E-4 ID:SGTC_1943|Compound:st076331|FD-Score:-3.19|P-value:1.88E-4 ID:SGTC_1947|Compound:st076332|FD-Score:-5.35|P-value:9.61E-10 ID:SGTC_2023|Compound:5144124|FD-Score:-3.06|P-value:3.24E-4 ID:SGTC_2125|Compound:5246583|FD-Score:2.82|P-value:5.65E-4 ID:SGTC_2255|Compound:7941087|FD-Score:2.81|P-value:5.79E-4 ID:SGTC_2259|Compound:7964009|FD-Score:2.89|P-value:4.23E-4 ID:SGTC_2305|Compound:7443522|FD-Score:2.73|P-value:8.08E-4 ID:SGTC_2352|Compound:9027995|FD-Score:-3.58|P-value:3.19E-5 ID:SGTC_2428|Compound:5615643|FD-Score:3.19|P-value:1.18E-4 ID:SGTC_2429|Compound:5902844|FD-Score:2.92|P-value:3.83E-4 ID:SGTC_2436|Compound:5633522|FD-Score:3.73|P-value:9.16E-6 ID:SGTC_2569|Compound:tetrandrine|FD-Score:2.70|P-value:9.18E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:4.50|P-value:1.27E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:-2.78|P-value:9.80E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:2.80|P-value:6.08E-4 ID:SGTC_2623|Compound:humulene|FD-Score:2.82|P-value:5.69E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-4.70|P-value:7.07E-8 ID:SGTC_2630|Compound:antimycin a|FD-Score:-4.56|P-value:1.65E-7 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:10.20|P-value:3.09E-31 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:4.49|P-value:1.30E-7 ID:SGTC_2867|Compound:9039098|FD-Score:-3.20|P-value:1.77E-4 ID:SGTC_2980|Compound:9014342|FD-Score:3.04|P-value:2.27E-4 ID:SGTC_3207|Compound:9114163|FD-Score:3.05|P-value:2.18E-4 ID:SGTC_3212|Compound:9128307|FD-Score:5.31|P-value:5.99E-10 ID:SGTC_3313|Compound:9133968|FD-Score:2.80|P-value:6.12E-4 ID:SGTC_3314|Compound:9135860|FD-Score:3.81|P-value:6.08E-6 ID:SGTC_3329|Compound:9141233|FD-Score:2.90|P-value:4.11E-4 ID:SGTC_3333|Compound:9141820|FD-Score:-3.77|P-value:1.28E-5

Top fitness defect scores for YKL116C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 10.20 100.0 µM 3.09E-31 2 1.60E-24
2 SGTC_514 4466-0038 8.30 2.6 nM 1.52E-21 cell wall 34 5.36E-17
3 SGTC_462 ag-879 5.91 158.0 µM 7.17E-12 5 1.73E-9
4 SGTC_3212 9128307 5.31 49.5 µM 5.99E-10 22 5.38E-8
5 SGTC_1083 didemethylchlorpromazine sulfoxide 4.72 707.4 nM 3.24E-8 redox potentiating 50 1.19E-6
6 SGTC_131 0141-0277 4.70 33.9 µM 3.73E-8 19 1.33E-6
7 SGTC_2578 7,8-dihydroxyflavone 4.50 64.4 µM 1.27E-7 23 3.46E-6
8 SGTC_2632 3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone 4.49 100.0 µM 1.30E-7 11 3.52E-6
9 SGTC_865 0488-0499 4.07 301.0 µM 1.47E-6 15 2.31E-5
10 SGTC_1153 4055-0057 3.88 267.0 µM 4.17E-6 27 5.22E-5
11 SGTC_3314 9135860 3.81 59.4 µM 6.08E-6 21 6.99E-5
12 SGTC_2436 5633522 3.73 8.1 µM 9.16E-6 22 9.62E-5
13 SGTC_471 1-acyl-paf 3.72 4.0 µM 9.39E-6 DNA intercalators 39 9.81E-5
14 SGTC_1239 0337-0584 3.70 35.4 µM 1.06E-5 unfolded protein response 68 1.07E-4
15 SGTC_113 0099-0221 3.68 2.5 µM 1.20E-5 33 1.19E-4
16 SGTC_1146 3579-1023 3.54 7.0 µM 2.32E-5 24 1.98E-4
17 SGTC_916 4466-0038 3.39 42.0 nM 4.85E-5 cell wall 101 3.52E-4
18 SGTC_1206 3978-0017 3.38 132.0 µM 5.00E-5 ERG2 58 3.61E-4
19 SGTC_1143 3454-2408 3.31 5.1 µM 7.06E-5 60S ribosome export 10 4.72E-4
20 SGTC_2428 5615643 3.19 7.8 µM 1.18E-4 54 7.03E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.249 1.29E-48 YMR036C MIH1 hom Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.206 1.51E-33 YGR211W ZPR1 het Essential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress
0.200 9.71E-32 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.196 2.38E-30 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.190 1.43E-28 YDR121W DPB4 hom Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
0.180 9.20E-26 YNL045W LAP2 hom Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
0.153 5.17E-19 YNR003C RPC34 het RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex
0.152 7.28E-19 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.151 1.55E-18 YHL003C LAG1 hom Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress
0.150 2.01E-18 YIL046W-A_p YIL046W-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.149 3.57E-18 YPL263C KEL3 hom Cytoplasmic protein of unknown function
0.146 2.13E-17 YJR130C STR2 hom Cystathionine gamma-synthase, converts cysteine into cystathionine
0.142 1.64E-16 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.140 4.65E-16 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.138 1.21E-15 YLL012W YEH1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes