YKL125W / RRN3

Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL125W / RRN3

Click on Significant Values for Screen Details ID:SGTC_200|Compound:k038-0079|FD-Score:3.31|P-value:8.41E-4 ID:SGTC_288|Compound:1315-0019|FD-Score:3.61|P-value:3.11E-4 ID:SGTC_317|Compound:4495-0002|FD-Score:4.25|P-value:2.73E-5 ID:SGTC_610|Compound:4215-0184|FD-Score:-4.44|P-value:1.12E-5 ID:SGTC_1027|Compound:k015-0025|FD-Score:4.14|P-value:4.22E-5 ID:SGTC_1034|Compound:k072-0165|FD-Score:-3.91|P-value:9.18E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-5.22|P-value:3.14E-7 ID:SGTC_1037|Compound:k284-1091|FD-Score:3.29|P-value:9.26E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.39|P-value:6.67E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:5.09|P-value:6.68E-7 ID:SGTC_1180|Compound:1193-0155|FD-Score:3.72|P-value:2.09E-4 ID:SGTC_1182|Compound:1222-0449|FD-Score:3.34|P-value:7.71E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:7.08|P-value:8.70E-12 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.34|P-value:7.77E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:3.48|P-value:4.85E-4 ID:SGTC_1250|Compound:0485-0349|FD-Score:4.72|P-value:3.81E-6 ID:SGTC_1380|Compound:2291-0730|FD-Score:3.68|P-value:2.39E-4 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.62|P-value:3.00E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:4.30|P-value:2.24E-5 ID:SGTC_1638|Compound:st008370|FD-Score:3.31|P-value:8.60E-4 ID:SGTC_1777|Compound:st045864|FD-Score:3.92|P-value:1.00E-4 ID:SGTC_2070|Compound:5230947|FD-Score:3.43|P-value:5.78E-4 ID:SGTC_2374|Compound:9071832|FD-Score:-3.75|P-value:1.65E-4 ID:SGTC_2470|Compound:5664817|FD-Score:4.04|P-value:6.42E-5 ID:SGTC_2618|Compound:avocadyne|FD-Score:4.74|P-value:3.44E-6 ID:SGTC_3062|Compound:9077494|FD-Score:-5.23|P-value:2.97E-7 ID:SGTC_3067|Compound:9117431|FD-Score:3.28|P-value:9.33E-4 ID:SGTC_3073|Compound:9114972|FD-Score:-4.56|P-value:6.72E-6 ID:SGTC_3189|Compound:9110682|FD-Score:-4.61|P-value:5.29E-6 ID:SGTC_3350|Compound:9150499|FD-Score:5.28|P-value:2.74E-7 ID:SGTC_200|Compound:k038-0079|FD-Score:3.31|P-value:8.41E-4 ID:SGTC_288|Compound:1315-0019|FD-Score:3.61|P-value:3.11E-4 ID:SGTC_317|Compound:4495-0002|FD-Score:4.25|P-value:2.73E-5 ID:SGTC_610|Compound:4215-0184|FD-Score:-4.44|P-value:1.12E-5 ID:SGTC_1027|Compound:k015-0025|FD-Score:4.14|P-value:4.22E-5 ID:SGTC_1034|Compound:k072-0165|FD-Score:-3.91|P-value:9.18E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-5.22|P-value:3.14E-7 ID:SGTC_1037|Compound:k284-1091|FD-Score:3.29|P-value:9.26E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.39|P-value:6.67E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:5.09|P-value:6.68E-7 ID:SGTC_1180|Compound:1193-0155|FD-Score:3.72|P-value:2.09E-4 ID:SGTC_1182|Compound:1222-0449|FD-Score:3.34|P-value:7.71E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:7.08|P-value:8.70E-12 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.34|P-value:7.77E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:3.48|P-value:4.85E-4 ID:SGTC_1250|Compound:0485-0349|FD-Score:4.72|P-value:3.81E-6 ID:SGTC_1380|Compound:2291-0730|FD-Score:3.68|P-value:2.39E-4 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.62|P-value:3.00E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:4.30|P-value:2.24E-5 ID:SGTC_1638|Compound:st008370|FD-Score:3.31|P-value:8.60E-4 ID:SGTC_1777|Compound:st045864|FD-Score:3.92|P-value:1.00E-4 ID:SGTC_2070|Compound:5230947|FD-Score:3.43|P-value:5.78E-4 ID:SGTC_2374|Compound:9071832|FD-Score:-3.75|P-value:1.65E-4 ID:SGTC_2470|Compound:5664817|FD-Score:4.04|P-value:6.42E-5 ID:SGTC_2618|Compound:avocadyne|FD-Score:4.74|P-value:3.44E-6 ID:SGTC_3062|Compound:9077494|FD-Score:-5.23|P-value:2.97E-7 ID:SGTC_3067|Compound:9117431|FD-Score:3.28|P-value:9.33E-4 ID:SGTC_3073|Compound:9114972|FD-Score:-4.56|P-value:6.72E-6 ID:SGTC_3189|Compound:9110682|FD-Score:-4.61|P-value:5.29E-6 ID:SGTC_3350|Compound:9150499|FD-Score:5.28|P-value:2.74E-7

Top fitness defect scores for YKL125W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1242 0402-0103 7.08 60.1 µM 8.70E-12 1 7.24E-13 2.05
2 SGTC_3350 9150499 5.28 3.6 µM 2.74E-7 cell wall 6 6.60E-8 0.10
3 SGTC_1051 3253-3903 5.09 78.0 µM 6.68E-7 2 1.77E-7 1.02
4 SGTC_2618 avocadyne 4.74 43.4 µM 3.44E-6 1 1.08E-6 0.69
5 SGTC_1250 0485-0349 4.72 40.2 µM 3.81E-6 6 1.20E-6 0.10
6 SGTC_1513 0337-0559 4.30 19.0 µM 2.24E-5 5 8.46E-6 0.23
7 SGTC_317 4495-0002 4.25 170.0 µM 2.73E-5 3 1.05E-5 0.36
8 SGTC_1027 k015-0025 4.14 8.7 µM 4.22E-5 4 1.70E-5 0.16
9 SGTC_2470 5664817 4.04 21.7 µM 6.42E-5 4 2.70E-5 0.35
10 SGTC_1777 st045864 3.92 34.8 µM 1.00E-4 3 4.41E-5 0.36
11 SGTC_1180 1193-0155 3.72 285.0 µM 2.09E-4 3 9.91E-5 0.23
12 SGTC_1380 2291-0730 3.68 164.0 µM 2.39E-4 6 1.15E-4 0.04
13 SGTC_1455 4262-0120 3.62 131.0 µM 3.00E-4 plasma membrane duress 7 1.48E-4 0.01
14 SGTC_288 1315-0019 3.61 35.1 µM 3.11E-4 RSC & ERG11 29 1.54E-4 0.07
15 SGTC_1248 0469-0687 3.48 58.6 µM 4.85E-4 11 2.51E-4 0.00
16 SGTC_2070 5230947 3.43 200.0 µM 5.78E-4 4 3.04E-4 0.06
17 SGTC_1047 2043-3044 3.39 82.0 µM 6.67E-4 15 3.55E-4 0.10
18 SGTC_1182 1222-0449 3.34 220.0 µM 7.71E-4 5 4.17E-4 0.08
19 SGTC_1247 0448-0043 3.34 5.8 µM 7.77E-4 ERG2 18 4.21E-4 0.16
20 SGTC_200 k038-0079 3.31 37.5 µM 8.41E-4 ubiquinone biosynthesis & proteasome 19 4.59E-4 0.08

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.125 3.70E-13 YNL161W CBK1 het Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
0.120 3.53E-12 YKR080W MTD1 hom NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline
0.103 2.27E-9 YMR288W HSH155 het U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155
0.097 1.80E-8 YDR297W SUR2 hom Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis
0.095 3.93E-8 YPR032W SRO7 hom Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication
0.085 8.26E-7 YCR087W_d YCR087W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene
0.083 1.31E-6 YGR185C TYS1 het Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress
0.083 1.47E-6 YIL079C AIR1 hom Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication
0.080 3.47E-6 YKL088W CAB3 het Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC
0.080 3.51E-6 YBR193C MED8 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.080 3.62E-6 YGL011C SCL1 het Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
0.080 3.95E-6 YKL083W_d YKL083W_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
0.078 6.45E-6 YCR013C_d YCR013C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant
0.077 7.15E-6 YBL004W UTP20 het Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
0.077 8.70E-6 YLR174W IDP2 hom Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication