YKL129C / MYO3

One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL129C / MYO3

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:-2.83|P-value:4.36E-4 ID:SGTC_368|Compound:fentichlor|FD-Score:2.83|P-value:3.80E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:2.63|P-value:8.49E-4 ID:SGTC_686|Compound:1319-0111|FD-Score:5.21|P-value:3.05E-10 ID:SGTC_705|Compound:1124-0212|FD-Score:3.59|P-value:9.57E-6 ID:SGTC_740|Compound:k014-0057|FD-Score:3.08|P-value:1.20E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:-2.76|P-value:5.84E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:2.60|P-value:9.84E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.07|P-value:1.27E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.09|P-value:1.18E-4 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:-3.70|P-value:6.46E-6 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:-2.97|P-value:2.35E-4 ID:SGTC_1549|Compound:4'-methoxyflavonol|FD-Score:-2.75|P-value:6.14E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:2.62|P-value:8.86E-4 ID:SGTC_1709|Compound:st033179|FD-Score:-3.24|P-value:6.60E-5 ID:SGTC_1729|Compound:st035615|FD-Score:3.05|P-value:1.42E-4 ID:SGTC_1734|Compound:st037168|FD-Score:3.37|P-value:3.00E-5 ID:SGTC_1760|Compound:st045453|FD-Score:2.70|P-value:6.54E-4 ID:SGTC_1860|Compound:st056228|FD-Score:2.60|P-value:9.80E-4 ID:SGTC_2016|Compound:5121758|FD-Score:-3.48|P-value:2.01E-5 ID:SGTC_2123|Compound:5259487|FD-Score:2.73|P-value:5.70E-4 ID:SGTC_2195|Compound:6536160|FD-Score:-3.22|P-value:7.30E-5 ID:SGTC_2218|Compound:6965428|FD-Score:3.25|P-value:5.32E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.25|P-value:5.39E-5 ID:SGTC_2435|Compound:5706476|FD-Score:3.37|P-value:3.08E-5 ID:SGTC_2456|Compound:5770359|FD-Score:3.34|P-value:3.58E-5 ID:SGTC_2466|Compound:5983409|FD-Score:2.76|P-value:5.06E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:2.73|P-value:5.58E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:2.82|P-value:3.92E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-2.63|P-value:9.63E-4 ID:SGTC_2650|Compound:prasterone|FD-Score:2.95|P-value:2.18E-4 ID:SGTC_2698|Compound:st077697|FD-Score:-3.50|P-value:1.87E-5 ID:SGTC_2782|Compound:7736369|FD-Score:-3.28|P-value:5.43E-5 ID:SGTC_2787|Compound:5284540|FD-Score:2.97|P-value:2.01E-4 ID:SGTC_2789|Compound:5281112|FD-Score:4.28|P-value:1.79E-7 ID:SGTC_2802|Compound:7925815|FD-Score:-2.81|P-value:4.59E-4 ID:SGTC_2816|Compound:7982706|FD-Score:3.17|P-value:7.97E-5 ID:SGTC_2882|Compound:9058044|FD-Score:3.32|P-value:3.81E-5 ID:SGTC_3109|Compound:9122853|FD-Score:2.78|P-value:4.64E-4 ID:SGTC_3310|Compound:9130229|FD-Score:-2.63|P-value:9.90E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:-2.83|P-value:4.36E-4 ID:SGTC_368|Compound:fentichlor|FD-Score:2.83|P-value:3.80E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:2.63|P-value:8.49E-4 ID:SGTC_686|Compound:1319-0111|FD-Score:5.21|P-value:3.05E-10 ID:SGTC_705|Compound:1124-0212|FD-Score:3.59|P-value:9.57E-6 ID:SGTC_740|Compound:k014-0057|FD-Score:3.08|P-value:1.20E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:-2.76|P-value:5.84E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:2.60|P-value:9.84E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.07|P-value:1.27E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.09|P-value:1.18E-4 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:-3.70|P-value:6.46E-6 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:-2.97|P-value:2.35E-4 ID:SGTC_1549|Compound:4'-methoxyflavonol|FD-Score:-2.75|P-value:6.14E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:2.62|P-value:8.86E-4 ID:SGTC_1709|Compound:st033179|FD-Score:-3.24|P-value:6.60E-5 ID:SGTC_1729|Compound:st035615|FD-Score:3.05|P-value:1.42E-4 ID:SGTC_1734|Compound:st037168|FD-Score:3.37|P-value:3.00E-5 ID:SGTC_1760|Compound:st045453|FD-Score:2.70|P-value:6.54E-4 ID:SGTC_1860|Compound:st056228|FD-Score:2.60|P-value:9.80E-4 ID:SGTC_2016|Compound:5121758|FD-Score:-3.48|P-value:2.01E-5 ID:SGTC_2123|Compound:5259487|FD-Score:2.73|P-value:5.70E-4 ID:SGTC_2195|Compound:6536160|FD-Score:-3.22|P-value:7.30E-5 ID:SGTC_2218|Compound:6965428|FD-Score:3.25|P-value:5.32E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.25|P-value:5.39E-5 ID:SGTC_2435|Compound:5706476|FD-Score:3.37|P-value:3.08E-5 ID:SGTC_2456|Compound:5770359|FD-Score:3.34|P-value:3.58E-5 ID:SGTC_2466|Compound:5983409|FD-Score:2.76|P-value:5.06E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:2.73|P-value:5.58E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:2.82|P-value:3.92E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-2.63|P-value:9.63E-4 ID:SGTC_2650|Compound:prasterone|FD-Score:2.95|P-value:2.18E-4 ID:SGTC_2698|Compound:st077697|FD-Score:-3.50|P-value:1.87E-5 ID:SGTC_2782|Compound:7736369|FD-Score:-3.28|P-value:5.43E-5 ID:SGTC_2787|Compound:5284540|FD-Score:2.97|P-value:2.01E-4 ID:SGTC_2789|Compound:5281112|FD-Score:4.28|P-value:1.79E-7 ID:SGTC_2802|Compound:7925815|FD-Score:-2.81|P-value:4.59E-4 ID:SGTC_2816|Compound:7982706|FD-Score:3.17|P-value:7.97E-5 ID:SGTC_2882|Compound:9058044|FD-Score:3.32|P-value:3.81E-5 ID:SGTC_3109|Compound:9122853|FD-Score:2.78|P-value:4.64E-4 ID:SGTC_3310|Compound:9130229|FD-Score:-2.63|P-value:9.90E-4

Top fitness defect scores for YKL129C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_686 1319-0111 5.21 510.0 µM 3.05E-10 60S ribosome export 7 9.65E-8
2 SGTC_2789 5281112 4.28 71.4 µM 1.79E-7 26 9.36E-6
3 SGTC_705 1124-0212 3.59 310.0 µM 9.57E-6 26 1.65E-4
4 SGTC_1734 st037168 3.37 69.4 µM 3.00E-5 47 3.76E-4
5 SGTC_2435 5706476 3.37 10.6 µM 3.08E-5 40 3.82E-4
6 SGTC_2456 5770359 3.34 74.5 µM 3.58E-5 33 4.26E-4
7 SGTC_2882 9058044 3.32 58.4 µM 3.81E-5 31 4.46E-4
8 SGTC_2218 6965428 3.25 193.0 µM 5.32E-5 TSC3-RPN4 19 5.69E-4
9 SGTC_2410 aloe-emodin-8-o-glycoside 3.25 46.3 µM 5.39E-5 mitochondrial processes 65 5.73E-4
10 SGTC_2816 7982706 3.17 58.4 µM 7.97E-5 azole & statin 91 7.61E-4
11 SGTC_1305 1181-0477 3.09 144.0 µM 1.18E-4 47 0.00101
12 SGTC_740 k014-0057 3.08 261.0 µM 1.20E-4 66 0.00102
13 SGTC_1261 0712-0010 3.07 12.7 µM 1.27E-4 RNA processing & uracil transport 60 0.00107
14 SGTC_1729 st035615 3.05 46.0 µM 1.42E-4 71 0.00116
15 SGTC_2787 5284540 2.97 71.4 µM 2.01E-4 73 0.00149
16 SGTC_2650 prasterone 2.95 100.0 µM 2.18E-4 29 0.00158
17 SGTC_368 fentichlor 2.83 4.6 µM 3.80E-4 85 0.00236
18 SGTC_2599 2',3-dihydroxy-4,4',6'-trimethoxychalcone 2.82 100.0 µM 3.92E-4 91 0.00242
19 SGTC_3109 9122853 2.78 49.5 µM 4.64E-4 51 0.00273
20 SGTC_2466 5983409 2.76 37.1 µM 5.06E-4 74 0.00291

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.118 6.29E-12 YDR196C CAB5 het Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies
0.117 1.02E-11 YDR117C TMA64 hom Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity
0.099 8.01E-9 YNL134C YNL134C hom Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
0.098 1.48E-8 YGR093W_p YGR093W_p hom Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
0.094 4.75E-8 YNR019W ARE2 hom Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication
0.089 2.74E-7 YML047W-A_d YML047W-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.087 4.24E-7 YLR098C CHA4 hom DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain
0.086 6.19E-7 YER024W YAT2 hom Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane
0.084 1.01E-6 YNL146W_p YNL146W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene
0.083 1.38E-6 YBR144C_d YBR144C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene
0.082 2.21E-6 YOR202W HIS3 hom Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p
0.081 2.91E-6 YBR222C PCS60 hom Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase
0.078 6.00E-6 YLR262C-A TMA7 hom Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress
0.074 1.73E-5 YKR002W PAP1 het Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping
0.074 1.89E-5 YMR223W UBP8 hom Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B