YKL132C / RMA1

Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL132C / RMA1

Click on Significant Values for Screen Details ID:SGTC_60|Compound:0986-0278|FD-Score:-3.19|P-value:4.19E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:3.35|P-value:1.81E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:3.46|P-value:1.15E-4 ID:SGTC_556|Compound:4058-0063|FD-Score:-2.97|P-value:9.50E-4 ID:SGTC_614|Compound:0462-0418|FD-Score:-3.44|P-value:1.57E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-4.49|P-value:1.26E-6 ID:SGTC_757|Compound:1066-0008|FD-Score:2.93|P-value:8.78E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:3.08|P-value:5.16E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:3.05|P-value:5.78E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:3.77|P-value:3.00E-5 ID:SGTC_1067|Compound:clotrimazole|FD-Score:4.38|P-value:1.63E-6 ID:SGTC_1303|Compound:1160-0052|FD-Score:-3.85|P-value:2.72E-5 ID:SGTC_1355|Compound:1499-0035|FD-Score:3.14|P-value:4.07E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.18|P-value:4.36E-4 ID:SGTC_1885|Compound:st057546|FD-Score:-3.41|P-value:1.76E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.14|P-value:5.15E-4 ID:SGTC_1973|Compound:st070275|FD-Score:3.06|P-value:5.51E-4 ID:SGTC_2015|Compound:5119600|FD-Score:-4.30|P-value:3.28E-6 ID:SGTC_2023|Compound:5144124|FD-Score:3.77|P-value:3.01E-5 ID:SGTC_2364|Compound:9055074|FD-Score:5.96|P-value:1.27E-10 ID:SGTC_2365|Compound:9042475|FD-Score:8.05|P-value:7.27E-18 ID:SGTC_2366|Compound:9070441|FD-Score:3.63|P-value:5.50E-5 ID:SGTC_2428|Compound:5615643|FD-Score:3.04|P-value:6.00E-4 ID:SGTC_2433|Compound:5607874|FD-Score:-3.00|P-value:8.51E-4 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:3.08|P-value:5.14E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.17|P-value:3.61E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:2.94|P-value:8.42E-4 ID:SGTC_2811|Compound:7967822|FD-Score:3.26|P-value:2.57E-4 ID:SGTC_2824|Compound:7998404|FD-Score:4.00|P-value:1.05E-5 ID:SGTC_2920|Compound:7982904|FD-Score:-3.35|P-value:2.24E-4 ID:SGTC_2942|Compound:9052777|FD-Score:3.43|P-value:1.31E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.66|P-value:4.80E-5 ID:SGTC_3179|Compound:9107801|FD-Score:-4.02|P-value:1.23E-5 ID:SGTC_3252|Compound:9135871|FD-Score:-3.19|P-value:4.28E-4 ID:SGTC_60|Compound:0986-0278|FD-Score:-3.19|P-value:4.19E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:3.35|P-value:1.81E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:3.46|P-value:1.15E-4 ID:SGTC_556|Compound:4058-0063|FD-Score:-2.97|P-value:9.50E-4 ID:SGTC_614|Compound:0462-0418|FD-Score:-3.44|P-value:1.57E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-4.49|P-value:1.26E-6 ID:SGTC_757|Compound:1066-0008|FD-Score:2.93|P-value:8.78E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:3.08|P-value:5.16E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:3.05|P-value:5.78E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:3.77|P-value:3.00E-5 ID:SGTC_1067|Compound:clotrimazole|FD-Score:4.38|P-value:1.63E-6 ID:SGTC_1303|Compound:1160-0052|FD-Score:-3.85|P-value:2.72E-5 ID:SGTC_1355|Compound:1499-0035|FD-Score:3.14|P-value:4.07E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.18|P-value:4.36E-4 ID:SGTC_1885|Compound:st057546|FD-Score:-3.41|P-value:1.76E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.14|P-value:5.15E-4 ID:SGTC_1973|Compound:st070275|FD-Score:3.06|P-value:5.51E-4 ID:SGTC_2015|Compound:5119600|FD-Score:-4.30|P-value:3.28E-6 ID:SGTC_2023|Compound:5144124|FD-Score:3.77|P-value:3.01E-5 ID:SGTC_2364|Compound:9055074|FD-Score:5.96|P-value:1.27E-10 ID:SGTC_2365|Compound:9042475|FD-Score:8.05|P-value:7.27E-18 ID:SGTC_2366|Compound:9070441|FD-Score:3.63|P-value:5.50E-5 ID:SGTC_2428|Compound:5615643|FD-Score:3.04|P-value:6.00E-4 ID:SGTC_2433|Compound:5607874|FD-Score:-3.00|P-value:8.51E-4 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:3.08|P-value:5.14E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.17|P-value:3.61E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:2.94|P-value:8.42E-4 ID:SGTC_2811|Compound:7967822|FD-Score:3.26|P-value:2.57E-4 ID:SGTC_2824|Compound:7998404|FD-Score:4.00|P-value:1.05E-5 ID:SGTC_2920|Compound:7982904|FD-Score:-3.35|P-value:2.24E-4 ID:SGTC_2942|Compound:9052777|FD-Score:3.43|P-value:1.31E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.66|P-value:4.80E-5 ID:SGTC_3179|Compound:9107801|FD-Score:-4.02|P-value:1.23E-5 ID:SGTC_3252|Compound:9135871|FD-Score:-3.19|P-value:4.28E-4

Top fitness defect scores for YKL132C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2365 9042475 8.05 200.0 µM 7.27E-18 2 4.16E-16
2 SGTC_2364 9055074 5.96 200.0 µM 1.27E-10 6 1.26E-9
3 SGTC_1067 clotrimazole 4.38 1.4 µM 1.63E-6 azole & statin 30 6.00E-6
4 SGTC_2824 7998404 4.00 52.0 µM 1.05E-5 35 3.17E-5
5 SGTC_998 3996-0085 3.77 177.0 µM 3.00E-5 26 8.15E-5
6 SGTC_2023 5144124 3.77 124.0 µM 3.01E-5 26 8.17E-5
7 SGTC_2949 9072542 3.66 2.2 µM 4.80E-5 TSC3-RPN4 46 1.24E-4
8 SGTC_2366 9070441 3.63 60.6 µM 5.50E-5 39 1.40E-4
9 SGTC_554 4483-2211 3.46 187.0 µM 1.15E-4 29 2.71E-4
10 SGTC_2942 9052777 3.43 62.2 µM 1.31E-4 57 3.05E-4
11 SGTC_553 k007-0256 3.35 45.7 µM 1.81E-4 heme biosynthesis & mitochondrial translocase 43 4.07E-4
12 SGTC_2811 7967822 3.26 32.5 µM 2.57E-4 37 5.57E-4
13 SGTC_2621 lithochol-11-enic acid 3.17 100.0 µM 3.61E-4 68 7.55E-4
14 SGTC_1355 1499-0035 3.14 1.7 µM 4.07E-4 53 8.43E-4
15 SGTC_2613 3',4'-dimethoxydalbergione 3.08 24.6 µM 5.14E-4 61 0.00104
16 SGTC_774 k060-0086 3.08 136.0 µM 5.16E-4 51 0.00104
17 SGTC_1973 st070275 3.06 50.2 µM 5.51E-4 38 0.00110
18 SGTC_963 1269-2853 3.05 151.0 µM 5.78E-4 64 0.00115
19 SGTC_2428 5615643 3.04 7.8 µM 6.00E-4 63 0.00119
20 SGTC_2722 naftopidil 2.94 47.2 µM 8.42E-4 44 0.00162

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.152 9.33E-19 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.149 4.92E-18 YHL006C SHU1 hom Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function
0.141 2.96E-16 YHR039C MSC7 hom Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids
0.136 2.32E-15 YEL056W HAT2 hom Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing
0.131 2.72E-14 YMR019W STB4 hom Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay;
0.130 4.52E-14 YKL168C KKQ8 hom Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication
0.129 6.03E-14 YER011W TIR1 hom Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking
0.126 2.66E-13 YOR010C TIR2 hom Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis
0.125 3.85E-13 YMR210W YMR210W hom Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification
0.123 7.57E-13 YKL220C FRE2 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
0.120 3.09E-12 YBR118W TEF2 hom Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication
0.119 4.44E-12 YER174C GRX4 hom Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5
0.117 1.02E-11 YNL097C-B_p YNL097C-B_p hom Putative protein of unknown function
0.112 9.05E-11 YOL141W PPM2 hom AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
0.109 2.15E-10 YGL134W PCL10 hom Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate