HIPHOP chemogenomics database

YKL133C_p

Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype)

Zygosity: Homozygous strain

fixedexpanded
Profile for YKL133C / YKL133C

Click on Significant Values for Screen Details

Top fitness defect scores for YKL133C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_527	4476-4215	6.62	268.0 µM	3.16E-10	PDR1	9	1.79E-11
2	SGTC_1002	4049-0206	6.53	172.0 µM	5.31E-10		3	3.25E-11
3	SGTC_1912	st060209	6.35	90.8 µM	1.54E-9		9	1.09E-10
4	SGTC_2566	crystal violet carbinol	5.88	226.0 nM	1.96E-8		10	1.99E-9
5	SGTC_2834	9001755	5.37	58.4 µM	2.71E-7		11	3.98E-8
6	SGTC_2969	9091408	5.08	28.7 µM	1.05E-6		9	1.87E-7
7	SGTC_154	3389-1638	4.77	511.1 µM	4.20E-6		11	9.02E-7
8	SGTC_540	1187-0821	4.72	17.3 µM	5.22E-6		7	1.16E-6
9	SGTC_1436	4092-1007	4.71	8.3 µM	5.51E-6		4	1.23E-6
10	SGTC_532	1548-0774	4.55	215.0 µM	1.11E-5		8	2.74E-6
11	SGTC_1144	3486-0183	4.41	12.5 µM	1.91E-5		16	5.07E-6
12	SGTC_881	0833-0569	4.21	170.0 µM	4.37E-5		19	1.30E-5
13	SGTC_770	0389-1121	4.15	67.2 µM	5.34E-5	RPP1 & pyrimidine depletion	23	1.63E-5
14	SGTC_2056	5235370	4.15	200.0 µM	5.48E-5		32	1.68E-5
15	SGTC_2089	5281248	4.11	129.7 µM	6.33E-5	superoxide	18	1.98E-5
16	SGTC_2401	5128683	3.87	200.0 µM	1.53E-4		43	5.40E-5
17	SGTC_3159	9100096	3.85	49.5 µM	1.67E-4		21	5.97E-5
18	SGTC_1249	0481-0015	3.79	10.2 µM	2.08E-4		28	7.67E-5
19	SGTC_994	3910-0338	3.73	138.0 µM	2.57E-4	calcium & mitochondrial duress	55	9.76E-5
20	SGTC_785	1270-0111	3.65	65.3 µM	3.30E-4		39	1.29E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.117	1.22E-11	YIR029W	DAL2	hom	Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
0.105	1.25E-9	YNL137C	NAM9	het	Mitochondrial ribosomal component of the small subunit
0.093	7.40E-8	YAL020C	ATS1	hom	Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle
0.088	3.56E-7	YNL292W	PUS4	hom	Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype)
0.086	5.88E-7	YHR017W	YSC83	hom	Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83
0.086	6.34E-7	YPL061W	ALD6	hom	Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress
0.084	1.19E-6	YOR028C	CIN5	hom	Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication
0.084	1.20E-6	YOR348C	PUT4	hom	Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells
0.082	1.74E-6	YOR233W	KIN4	hom	Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication
0.081	2.79E-6	YMR199W	CLN1	hom	G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p)
0.080	3.69E-6	YLR004C	THI73	hom	Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs
0.077	8.21E-6	YBR079C	RPG1	het	eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation
0.075	1.30E-5	YNL102W	POL1	het	Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis
0.072	3.35E-5	YGR224W	AZR1	hom	Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole
0.071	3.94E-5	YJL082W	IML2	hom	Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication