YKL135C / APL2

Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL135C / APL2

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:5.46|P-value:2.19E-6 ID:SGTC_70|Compound:0141-0289|FD-Score:3.78|P-value:7.29E-4 ID:SGTC_84|Compound:0443-0269|FD-Score:-4.10|P-value:2.81E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:7.44|P-value:2.00E-10 ID:SGTC_201|Compound:4262-0159|FD-Score:7.91|P-value:1.47E-11 ID:SGTC_207|Compound:4185-0010|FD-Score:13.20|P-value:9.03E-29 ID:SGTC_252|Compound:miconazole|FD-Score:9.47|P-value:8.38E-16 ID:SGTC_254|Compound:nsc-64875|FD-Score:12.10|P-value:8.78E-25 ID:SGTC_262|Compound:fenpropimorph|FD-Score:6.61|P-value:1.37E-8 ID:SGTC_308|Compound:4092-1169|FD-Score:4.34|P-value:1.30E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:9.86|P-value:5.70E-17 ID:SGTC_320|Compound:4092-0846|FD-Score:8.61|P-value:2.18E-13 ID:SGTC_363|Compound:0455-0021|FD-Score:4.02|P-value:3.60E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:-3.71|P-value:9.07E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-8.21|P-value:2.62E-12 ID:SGTC_459|Compound:nigericin|FD-Score:6.40|P-value:3.67E-8 ID:SGTC_502|Compound:l-744,832|FD-Score:4.88|P-value:2.07E-5 ID:SGTC_515|Compound:4092-1064|FD-Score:7.16|P-value:8.69E-10 ID:SGTC_568|Compound:1193-0097|FD-Score:4.27|P-value:1.64E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:14.20|P-value:2.37E-33 ID:SGTC_689|Compound:0833-0039|FD-Score:6.67|P-value:1.03E-8 ID:SGTC_768|Compound:0384-0033|FD-Score:16.90|P-value:4.89E-46 ID:SGTC_771|Compound:0250-0039|FD-Score:4.44|P-value:9.34E-5 ID:SGTC_788|Compound:1182-0090|FD-Score:10.20|P-value:3.39E-18 ID:SGTC_902|Compound:1120-0002|FD-Score:3.75|P-value:8.02E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-3.71|P-value:9.18E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:4.28|P-value:1.59E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:4.26|P-value:1.70E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:5.74|P-value:6.96E-7 ID:SGTC_1057|Compound:3448-5381|FD-Score:4.52|P-value:7.10E-5 ID:SGTC_1072|Compound:oxiconazole|FD-Score:6.46|P-value:2.81E-8 ID:SGTC_1233|Compound:0325-0408|FD-Score:4.71|P-value:3.79E-5 ID:SGTC_1308|Compound:1187-1617|FD-Score:4.33|P-value:1.35E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.71|P-value:8.99E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:4.01|P-value:3.74E-4 ID:SGTC_1547|Compound:harmine|FD-Score:3.91|P-value:4.99E-4 ID:SGTC_1687|Compound:st020778|FD-Score:6.67|P-value:1.00E-8 ID:SGTC_1712|Compound:st032288|FD-Score:4.86|P-value:2.15E-5 ID:SGTC_1745|Compound:st081495|FD-Score:4.42|P-value:1.00E-4 ID:SGTC_1750|Compound:st081859|FD-Score:22.60|P-value:6.96E-81 ID:SGTC_1797|Compound:st048695|FD-Score:5.83|P-value:4.81E-7 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.94|P-value:4.57E-4 ID:SGTC_1930|Compound:st074634|FD-Score:4.28|P-value:1.60E-4 ID:SGTC_2006|Compound:4011487|FD-Score:12.60|P-value:2.33E-26 ID:SGTC_2020|Compound:4017018|FD-Score:-3.70|P-value:9.39E-4 ID:SGTC_2021|Compound:4019055|FD-Score:5.61|P-value:1.19E-6 ID:SGTC_2036|Compound:5160441|FD-Score:4.67|P-value:4.30E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:6.07|P-value:1.63E-7 ID:SGTC_2275|Compound:7947463|FD-Score:4.02|P-value:3.65E-4 ID:SGTC_2378|Compound:9074213|FD-Score:-4.40|P-value:1.08E-4 ID:SGTC_2389|Compound:9091542|FD-Score:3.94|P-value:4.62E-4 ID:SGTC_2470|Compound:5664817|FD-Score:3.96|P-value:4.36E-4 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:10.40|P-value:1.04E-18 ID:SGTC_2586|Compound:curcumin|FD-Score:5.22|P-value:5.57E-6 ID:SGTC_2602|Compound:kawain|FD-Score:-3.81|P-value:6.75E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:7.80|P-value:2.73E-11 ID:SGTC_2776|Compound:terbinafine|FD-Score:3.94|P-value:4.64E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:5.18|P-value:6.53E-6 ID:SGTC_2790|Compound:5423970|FD-Score:5.37|P-value:3.12E-6 ID:SGTC_2830|Compound:7992007|FD-Score:3.90|P-value:5.18E-4 ID:SGTC_2831|Compound:7999036|FD-Score:-4.00|P-value:3.85E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-5.05|P-value:1.08E-5 ID:SGTC_3018|Compound:9082602|FD-Score:6.20|P-value:9.25E-8 ID:SGTC_3308|Compound:9128332|FD-Score:13.90|P-value:9.65E-32 ID:SGTC_3324|Compound:9138759|FD-Score:-3.97|P-value:4.26E-4 ID:SGTC_3325|Compound:9138760|FD-Score:5.55|P-value:1.52E-6 ID:SGTC_3350|Compound:9150499|FD-Score:4.57|P-value:6.08E-5 ID:SGTC_15|Compound:0266-0086|FD-Score:5.46|P-value:2.19E-6 ID:SGTC_70|Compound:0141-0289|FD-Score:3.78|P-value:7.29E-4 ID:SGTC_84|Compound:0443-0269|FD-Score:-4.10|P-value:2.81E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:7.44|P-value:2.00E-10 ID:SGTC_201|Compound:4262-0159|FD-Score:7.91|P-value:1.47E-11 ID:SGTC_207|Compound:4185-0010|FD-Score:13.20|P-value:9.03E-29 ID:SGTC_252|Compound:miconazole|FD-Score:9.47|P-value:8.38E-16 ID:SGTC_254|Compound:nsc-64875|FD-Score:12.10|P-value:8.78E-25 ID:SGTC_262|Compound:fenpropimorph|FD-Score:6.61|P-value:1.37E-8 ID:SGTC_308|Compound:4092-1169|FD-Score:4.34|P-value:1.30E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:9.86|P-value:5.70E-17 ID:SGTC_320|Compound:4092-0846|FD-Score:8.61|P-value:2.18E-13 ID:SGTC_363|Compound:0455-0021|FD-Score:4.02|P-value:3.60E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:-3.71|P-value:9.07E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-8.21|P-value:2.62E-12 ID:SGTC_459|Compound:nigericin|FD-Score:6.40|P-value:3.67E-8 ID:SGTC_502|Compound:l-744,832|FD-Score:4.88|P-value:2.07E-5 ID:SGTC_515|Compound:4092-1064|FD-Score:7.16|P-value:8.69E-10 ID:SGTC_568|Compound:1193-0097|FD-Score:4.27|P-value:1.64E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:14.20|P-value:2.37E-33 ID:SGTC_689|Compound:0833-0039|FD-Score:6.67|P-value:1.03E-8 ID:SGTC_768|Compound:0384-0033|FD-Score:16.90|P-value:4.89E-46 ID:SGTC_771|Compound:0250-0039|FD-Score:4.44|P-value:9.34E-5 ID:SGTC_788|Compound:1182-0090|FD-Score:10.20|P-value:3.39E-18 ID:SGTC_902|Compound:1120-0002|FD-Score:3.75|P-value:8.02E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-3.71|P-value:9.18E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:4.28|P-value:1.59E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:4.26|P-value:1.70E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:5.74|P-value:6.96E-7 ID:SGTC_1057|Compound:3448-5381|FD-Score:4.52|P-value:7.10E-5 ID:SGTC_1072|Compound:oxiconazole|FD-Score:6.46|P-value:2.81E-8 ID:SGTC_1233|Compound:0325-0408|FD-Score:4.71|P-value:3.79E-5 ID:SGTC_1308|Compound:1187-1617|FD-Score:4.33|P-value:1.35E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.71|P-value:8.99E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:4.01|P-value:3.74E-4 ID:SGTC_1547|Compound:harmine|FD-Score:3.91|P-value:4.99E-4 ID:SGTC_1687|Compound:st020778|FD-Score:6.67|P-value:1.00E-8 ID:SGTC_1712|Compound:st032288|FD-Score:4.86|P-value:2.15E-5 ID:SGTC_1745|Compound:st081495|FD-Score:4.42|P-value:1.00E-4 ID:SGTC_1750|Compound:st081859|FD-Score:22.60|P-value:6.96E-81 ID:SGTC_1797|Compound:st048695|FD-Score:5.83|P-value:4.81E-7 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.94|P-value:4.57E-4 ID:SGTC_1930|Compound:st074634|FD-Score:4.28|P-value:1.60E-4 ID:SGTC_2006|Compound:4011487|FD-Score:12.60|P-value:2.33E-26 ID:SGTC_2020|Compound:4017018|FD-Score:-3.70|P-value:9.39E-4 ID:SGTC_2021|Compound:4019055|FD-Score:5.61|P-value:1.19E-6 ID:SGTC_2036|Compound:5160441|FD-Score:4.67|P-value:4.30E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:6.07|P-value:1.63E-7 ID:SGTC_2275|Compound:7947463|FD-Score:4.02|P-value:3.65E-4 ID:SGTC_2378|Compound:9074213|FD-Score:-4.40|P-value:1.08E-4 ID:SGTC_2389|Compound:9091542|FD-Score:3.94|P-value:4.62E-4 ID:SGTC_2470|Compound:5664817|FD-Score:3.96|P-value:4.36E-4 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:10.40|P-value:1.04E-18 ID:SGTC_2586|Compound:curcumin|FD-Score:5.22|P-value:5.57E-6 ID:SGTC_2602|Compound:kawain|FD-Score:-3.81|P-value:6.75E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:7.80|P-value:2.73E-11 ID:SGTC_2776|Compound:terbinafine|FD-Score:3.94|P-value:4.64E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:5.18|P-value:6.53E-6 ID:SGTC_2790|Compound:5423970|FD-Score:5.37|P-value:3.12E-6 ID:SGTC_2830|Compound:7992007|FD-Score:3.90|P-value:5.18E-4 ID:SGTC_2831|Compound:7999036|FD-Score:-4.00|P-value:3.85E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-5.05|P-value:1.08E-5 ID:SGTC_3018|Compound:9082602|FD-Score:6.20|P-value:9.25E-8 ID:SGTC_3308|Compound:9128332|FD-Score:13.90|P-value:9.65E-32 ID:SGTC_3324|Compound:9138759|FD-Score:-3.97|P-value:4.26E-4 ID:SGTC_3325|Compound:9138760|FD-Score:5.55|P-value:1.52E-6 ID:SGTC_3350|Compound:9150499|FD-Score:4.57|P-value:6.08E-5

Top fitness defect scores for YKL135C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1750 st081859 22.60 2.4 µM 6.96E-81 endomembrane recycling 10 1.71E-113
2 SGTC_768 0384-0033 16.90 77.3 µM 4.89E-46 fatty acid desaturase (OLE1) 6 3.00E-64
3 SGTC_649 1123-0311 14.20 367.0 µM 2.37E-33 endomembrane recycling 5 2.46E-46
4 SGTC_3308 9128332 13.90 17.6 µM 9.65E-32 plasma membrane duress 14 4.61E-44
5 SGTC_207 4185-0010 13.20 4.5 µM 9.03E-29 endomembrane recycling 10 7.21E-40
6 SGTC_2006 4011487 12.60 200.0 µM 2.33E-26 endomembrane recycling 6 1.82E-36
7 SGTC_254 nsc-64875 12.10 3.8 µM 8.78E-25 ergosterol depletion effects on membrane 6 3.05E-34
8 SGTC_2565 isoliquiritigenin 10.40 100.0 µM 1.04E-18 2 1.12E-25
9 SGTC_788 1182-0090 10.20 188.0 µM 3.39E-18 endomembrane recycling 12 5.94E-25
10 SGTC_312 4092-0653 9.86 6.8 µM 5.70E-17 ergosterol depletion effects on membrane 13 3.16E-23
11 SGTC_252 miconazole 9.47 1.3 nM 8.38E-16 ergosterol depletion effects on membrane 4 1.39E-21
12 SGTC_320 4092-0846 8.61 5.3 µM 2.18E-13 ergosterol depletion effects on membrane 6 3.51E-18
13 SGTC_201 4262-0159 7.91 12.8 µM 1.47E-11 ergosterol depletion effects on membrane 11 1.31E-15
14 SGTC_2619 obliquin 7.80 87.9 µM 2.73E-11 cell wall 46 3.13E-15
15 SGTC_178 4092-0806 7.44 87.5 µM 2.00E-10 ergosterol depletion effects on membrane 22 5.14E-14
16 SGTC_515 4092-1064 7.16 102.0 µM 8.69E-10 plasma membrane duress 21 4.04E-13
17 SGTC_1687 st020778 6.67 55.4 µM 1.00E-8 94 1.25E-11
18 SGTC_689 0833-0039 6.67 98.0 µM 1.03E-8 ergosterol depletion effects on membrane 12 1.30E-11
19 SGTC_262 fenpropimorph 6.61 62.5 µM 1.37E-8 ergosterol depletion effects on membrane 9 1.94E-11
20 SGTC_1072 oxiconazole 6.46 14.9 nM 2.81E-8 ergosterol depletion effects on membrane 20 5.30E-11

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.592 2.91E-316 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.563 6.41E-280 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.523 1.45E-235 YHL019C APM2 hom Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.513 3.01E-225 YLR169W_d YLR169W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.498 2.17E-209 YMR071C TVP18 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
0.481 1.52E-193 YJL207C LAA1 hom AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene
0.472 2.65E-185 YLR171W_d YLR171W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.456 1.30E-171 YER031C YPT31 hom Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication
0.442 1.77E-160 YBL010C_p YBL010C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
0.436 6.52E-156 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.406 1.98E-133 YDL226C GCS1 hom ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
0.396 1.57E-126 YKL136W_d YKL136W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C
0.383 1.30E-117 YJL204C RCY1 hom F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
0.338 2.89E-90 YLR350W ORM2 hom Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress
0.334 5.12E-88 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene