YKL142W / MRP8

Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL142W / MRP8

Click on Significant Values for Screen Details ID:SGTC_50|Compound:0929-0078|FD-Score:4.34|P-value:1.14E-5 ID:SGTC_139|Compound:0099-0334|FD-Score:4.63|P-value:3.12E-6 ID:SGTC_151|Compound:3013-0144|FD-Score:3.71|P-value:1.45E-4 ID:SGTC_181|Compound:1447-1644|FD-Score:-4.40|P-value:1.27E-5 ID:SGTC_263|Compound:alizarin|FD-Score:4.19|P-value:2.17E-5 ID:SGTC_305|Compound:1682-2595|FD-Score:-3.28|P-value:8.73E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.77|P-value:1.53E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.68|P-value:1.58E-4 ID:SGTC_575|Compound:k072-0246|FD-Score:3.78|P-value:1.09E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:4.77|P-value:1.65E-6 ID:SGTC_788|Compound:1182-0090|FD-Score:4.13|P-value:2.72E-5 ID:SGTC_792|Compound:4534-1957|FD-Score:3.60|P-value:2.12E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:-3.70|P-value:1.99E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-4.53|P-value:7.13E-6 ID:SGTC_930|Compound:2810-3379|FD-Score:-3.43|P-value:5.17E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:-3.87|P-value:1.07E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:-3.55|P-value:3.46E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:3.21|P-value:8.19E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:3.96|P-value:5.50E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.27|P-value:6.73E-4 ID:SGTC_1384|Compound:3089-0840|FD-Score:-3.31|P-value:7.89E-4 ID:SGTC_1480|Compound:1070-0044|FD-Score:3.77|P-value:1.15E-4 ID:SGTC_1619|Compound:st003326|FD-Score:4.83|P-value:1.22E-6 ID:SGTC_1752|Compound:st044819|FD-Score:3.17|P-value:9.55E-4 ID:SGTC_1780|Compound:st038448|FD-Score:3.30|P-value:6.25E-4 ID:SGTC_1807|Compound:st050092|FD-Score:-3.40|P-value:5.85E-4 ID:SGTC_1922|Compound:5578187|FD-Score:4.16|P-value:2.44E-5 ID:SGTC_2067|Compound:5224629|FD-Score:-4.16|P-value:3.44E-5 ID:SGTC_2073|Compound:5212005|FD-Score:-3.70|P-value:1.99E-4 ID:SGTC_2080|Compound:5212524|FD-Score:-3.96|P-value:7.43E-5 ID:SGTC_2102|Compound:5377816|FD-Score:4.83|P-value:1.22E-6 ID:SGTC_2149|Compound:5554786|FD-Score:4.84|P-value:1.17E-6 ID:SGTC_2166|Compound:5933061|FD-Score:-3.95|P-value:7.82E-5 ID:SGTC_2238|Compound:6671620|FD-Score:4.11|P-value:3.00E-5 ID:SGTC_2393|Compound:9031399|FD-Score:-4.78|P-value:2.34E-6 ID:SGTC_2399|Compound:5884133|FD-Score:4.33|P-value:1.16E-5 ID:SGTC_2402|Compound:5553605|FD-Score:7.03|P-value:3.91E-12 ID:SGTC_2414|Compound:st077232|FD-Score:-3.29|P-value:8.31E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:3.55|P-value:2.60E-4 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:3.36|P-value:4.98E-4 ID:SGTC_2709|Compound:st078560|FD-Score:-3.79|P-value:1.42E-4 ID:SGTC_2725|Compound:haloperidol|FD-Score:3.81|P-value:9.72E-5 ID:SGTC_2755|Compound:lomerizine|FD-Score:4.24|P-value:1.72E-5 ID:SGTC_2903|Compound:5923464|FD-Score:3.36|P-value:4.94E-4 ID:SGTC_2938|Compound:9059746|FD-Score:-6.45|P-value:2.79E-10 ID:SGTC_2984|Compound:9022109|FD-Score:3.25|P-value:7.40E-4 ID:SGTC_3052|Compound:9092717|FD-Score:3.18|P-value:9.29E-4 ID:SGTC_3112|Compound:9123419|FD-Score:3.41|P-value:4.19E-4 ID:SGTC_3145|Compound:9085516|FD-Score:7.40|P-value:3.11E-13 ID:SGTC_3158|Compound:9100007|FD-Score:5.12|P-value:3.06E-7 ID:SGTC_3162|Compound:9099653|FD-Score:-3.53|P-value:3.75E-4 ID:SGTC_3168|Compound:9103419|FD-Score:-3.24|P-value:9.78E-4 ID:SGTC_3200|Compound:9114385|FD-Score:3.40|P-value:4.42E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:4.34|P-value:1.14E-5 ID:SGTC_139|Compound:0099-0334|FD-Score:4.63|P-value:3.12E-6 ID:SGTC_151|Compound:3013-0144|FD-Score:3.71|P-value:1.45E-4 ID:SGTC_181|Compound:1447-1644|FD-Score:-4.40|P-value:1.27E-5 ID:SGTC_263|Compound:alizarin|FD-Score:4.19|P-value:2.17E-5 ID:SGTC_305|Compound:1682-2595|FD-Score:-3.28|P-value:8.73E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.77|P-value:1.53E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.68|P-value:1.58E-4 ID:SGTC_575|Compound:k072-0246|FD-Score:3.78|P-value:1.09E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:4.77|P-value:1.65E-6 ID:SGTC_788|Compound:1182-0090|FD-Score:4.13|P-value:2.72E-5 ID:SGTC_792|Compound:4534-1957|FD-Score:3.60|P-value:2.12E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:-3.70|P-value:1.99E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-4.53|P-value:7.13E-6 ID:SGTC_930|Compound:2810-3379|FD-Score:-3.43|P-value:5.17E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:-3.87|P-value:1.07E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:-3.55|P-value:3.46E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:3.21|P-value:8.19E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:3.96|P-value:5.50E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.27|P-value:6.73E-4 ID:SGTC_1384|Compound:3089-0840|FD-Score:-3.31|P-value:7.89E-4 ID:SGTC_1480|Compound:1070-0044|FD-Score:3.77|P-value:1.15E-4 ID:SGTC_1619|Compound:st003326|FD-Score:4.83|P-value:1.22E-6 ID:SGTC_1752|Compound:st044819|FD-Score:3.17|P-value:9.55E-4 ID:SGTC_1780|Compound:st038448|FD-Score:3.30|P-value:6.25E-4 ID:SGTC_1807|Compound:st050092|FD-Score:-3.40|P-value:5.85E-4 ID:SGTC_1922|Compound:5578187|FD-Score:4.16|P-value:2.44E-5 ID:SGTC_2067|Compound:5224629|FD-Score:-4.16|P-value:3.44E-5 ID:SGTC_2073|Compound:5212005|FD-Score:-3.70|P-value:1.99E-4 ID:SGTC_2080|Compound:5212524|FD-Score:-3.96|P-value:7.43E-5 ID:SGTC_2102|Compound:5377816|FD-Score:4.83|P-value:1.22E-6 ID:SGTC_2149|Compound:5554786|FD-Score:4.84|P-value:1.17E-6 ID:SGTC_2166|Compound:5933061|FD-Score:-3.95|P-value:7.82E-5 ID:SGTC_2238|Compound:6671620|FD-Score:4.11|P-value:3.00E-5 ID:SGTC_2393|Compound:9031399|FD-Score:-4.78|P-value:2.34E-6 ID:SGTC_2399|Compound:5884133|FD-Score:4.33|P-value:1.16E-5 ID:SGTC_2402|Compound:5553605|FD-Score:7.03|P-value:3.91E-12 ID:SGTC_2414|Compound:st077232|FD-Score:-3.29|P-value:8.31E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:3.55|P-value:2.60E-4 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:3.36|P-value:4.98E-4 ID:SGTC_2709|Compound:st078560|FD-Score:-3.79|P-value:1.42E-4 ID:SGTC_2725|Compound:haloperidol|FD-Score:3.81|P-value:9.72E-5 ID:SGTC_2755|Compound:lomerizine|FD-Score:4.24|P-value:1.72E-5 ID:SGTC_2903|Compound:5923464|FD-Score:3.36|P-value:4.94E-4 ID:SGTC_2938|Compound:9059746|FD-Score:-6.45|P-value:2.79E-10 ID:SGTC_2984|Compound:9022109|FD-Score:3.25|P-value:7.40E-4 ID:SGTC_3052|Compound:9092717|FD-Score:3.18|P-value:9.29E-4 ID:SGTC_3112|Compound:9123419|FD-Score:3.41|P-value:4.19E-4 ID:SGTC_3145|Compound:9085516|FD-Score:7.40|P-value:3.11E-13 ID:SGTC_3158|Compound:9100007|FD-Score:5.12|P-value:3.06E-7 ID:SGTC_3162|Compound:9099653|FD-Score:-3.53|P-value:3.75E-4 ID:SGTC_3168|Compound:9103419|FD-Score:-3.24|P-value:9.78E-4 ID:SGTC_3200|Compound:9114385|FD-Score:3.40|P-value:4.42E-4

Top fitness defect scores for YKL142W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3145 9085516 7.40 49.5 µM 3.11E-13 fatty acid desaturase (OLE1) 2 6.85E-14
2 SGTC_2402 5553605 7.03 200.0 µM 3.91E-12 2 1.01E-12
3 SGTC_3158 9100007 5.12 49.5 µM 3.06E-7 7 1.56E-7
4 SGTC_2149 5554786 4.84 194.8 µM 1.17E-6 cell wall signaling 19 6.46E-7
5 SGTC_1619 st003326 4.83 88.7 µM 1.22E-6 60S ribosome export 6 6.75E-7
6 SGTC_2102 5377816 4.83 72.8 µM 1.22E-6 9 6.78E-7
7 SGTC_704 1310-0270 4.77 142.0 µM 1.65E-6 20 9.32E-7
8 SGTC_139 0099-0334 4.63 718.6 µM 3.12E-6 14 1.82E-6
9 SGTC_50 0929-0078 4.34 56.4 µM 1.14E-5 35 7.19E-6
10 SGTC_2399 5884133 4.33 200.0 µM 1.16E-5 cell wall signaling 58 7.33E-6
11 SGTC_2755 lomerizine 4.24 42.7 µM 1.72E-5 21 1.11E-5
12 SGTC_263 alizarin 4.19 107.9 µM 2.17E-5 iron homeostasis 51 1.42E-5
13 SGTC_1922 5578187 4.16 25.0 µM 2.44E-5 TRP & mitochondrial translation 24 1.60E-5
14 SGTC_788 1182-0090 4.13 188.0 µM 2.72E-5 endomembrane recycling 73 1.80E-5
15 SGTC_2238 6671620 4.11 73.2 µM 3.00E-5 25 2.00E-5
16 SGTC_1063 disulfiram 3.96 1.8 µM 5.50E-5 23 3.79E-5
17 SGTC_2725 haloperidol 3.81 53.2 µM 9.72E-5 plasma membrane duress 37 6.91E-5
18 SGTC_575 k072-0246 3.78 11.9 µM 1.09E-4 24 7.82E-5
19 SGTC_1480 1070-0044 3.77 41.2 µM 1.15E-4 24 8.24E-5
20 SGTC_151 3013-0144 3.71 2.3 µM 1.45E-4 iron homeostasis 38 1.06E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.099 9.02E-9 YCR014C POL4 hom DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta
0.093 6.50E-8 YLR432W IMD3 hom Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed
0.093 7.34E-8 YOR287C RRP36 het Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit
0.091 1.11E-7 YLR051C FCF2 het Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon
0.089 2.08E-7 YLR361C DCR2 hom Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START
0.080 3.48E-6 YJL028W YJL028W hom Protein of unknown function; may interact with ribosomes, based on co-purification experiments
0.080 3.60E-6 YBL084C CDC27 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.078 5.31E-6 YOR343C_d YOR343C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.078 6.75E-6 YFR038W IRC5 hom Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci
0.078 6.87E-6 YGR126W_p YGR126W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
0.076 1.11E-5 YMR213W CEF1 het Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p
0.074 1.74E-5 YPL199C_p YPL199C_p hom Putative protein of unknown function, predicted to be palmitoylated
0.074 1.99E-5 YNL209W SSB2 hom Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication
0.072 2.94E-5 YDR270W CCC2 hom Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism
0.072 3.38E-5 YKL079W SMY1 hom Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins