YKL145W / RPT1

One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL145W / RPT1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:10.70|P-value:3.45E-19 ID:SGTC_138|Compound:0850-0259|FD-Score:6.83|P-value:6.50E-9 ID:SGTC_163|Compound:1327-0036|FD-Score:-4.70|P-value:4.49E-5 ID:SGTC_197|Compound:4544-0043|FD-Score:4.77|P-value:3.56E-5 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.96|P-value:1.84E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:4.06|P-value:3.68E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:3.77|P-value:8.56E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:6.71|P-value:1.18E-8 ID:SGTC_466|Compound:geldanamycin|FD-Score:5.76|P-value:8.01E-7 ID:SGTC_550|Compound:4123-0385|FD-Score:4.52|P-value:8.36E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:4.95|P-value:1.88E-5 ID:SGTC_618|Compound:k072-0232|FD-Score:-3.83|P-value:7.04E-4 ID:SGTC_657|Compound:3534-0529|FD-Score:3.72|P-value:9.70E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:4.18|P-value:2.50E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:4.61|P-value:6.17E-5 ID:SGTC_908|Compound:3448-7491|FD-Score:-4.60|P-value:6.33E-5 ID:SGTC_909|Compound:3448-9333|FD-Score:-3.83|P-value:7.03E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:3.81|P-value:7.61E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:8.91|P-value:5.82E-14 ID:SGTC_923|Compound:1900-2434|FD-Score:-3.77|P-value:8.32E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:4.17|P-value:2.57E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:3.71|P-value:9.99E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:-3.88|P-value:6.12E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.48|P-value:9.75E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:4.27|P-value:1.91E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:4.16|P-value:2.69E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:4.18|P-value:2.54E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:4.54|P-value:7.79E-5 ID:SGTC_1510|Compound:1123-0200|FD-Score:4.40|P-value:1.23E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:4.10|P-value:3.20E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:8.03|P-value:1.16E-11 ID:SGTC_1605|Compound:st000082|FD-Score:-4.57|P-value:7.12E-5 ID:SGTC_1672|Compound:st016598|FD-Score:5.43|P-value:3.04E-6 ID:SGTC_1700|Compound:st030840|FD-Score:4.74|P-value:3.91E-5 ID:SGTC_1754|Compound:st042065|FD-Score:5.21|P-value:7.11E-6 ID:SGTC_1800|Compound:st050079|FD-Score:8.06|P-value:9.61E-12 ID:SGTC_1803|Compound:st050068|FD-Score:4.90|P-value:2.22E-5 ID:SGTC_1818|Compound:st052136|FD-Score:6.76|P-value:9.12E-9 ID:SGTC_1827|Compound:berberine|FD-Score:-5.67|P-value:1.16E-6 ID:SGTC_1829|Compound:berberine|FD-Score:-8.28|P-value:2.72E-12 ID:SGTC_1836|Compound:st055642|FD-Score:5.60|P-value:1.57E-6 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.55|P-value:7.64E-5 ID:SGTC_1845|Compound:st056753|FD-Score:4.16|P-value:2.64E-4 ID:SGTC_1959|Compound:st077091|FD-Score:4.34|P-value:1.51E-4 ID:SGTC_1987|Compound:st072290|FD-Score:4.92|P-value:2.07E-5 ID:SGTC_2090|Compound:5301565|FD-Score:-3.79|P-value:7.95E-4 ID:SGTC_2096|Compound:5468907|FD-Score:3.92|P-value:5.43E-4 ID:SGTC_2100|Compound:5377183|FD-Score:-3.80|P-value:7.64E-4 ID:SGTC_2101|Compound:5377231|FD-Score:4.42|P-value:1.17E-4 ID:SGTC_2321|Compound:7390090|FD-Score:5.83|P-value:6.03E-7 ID:SGTC_2322|Compound:7413319|FD-Score:4.16|P-value:2.68E-4 ID:SGTC_2328|Compound:6201408|FD-Score:-3.82|P-value:7.27E-4 ID:SGTC_2445|Compound:5742597|FD-Score:3.92|P-value:5.59E-4 ID:SGTC_2477|Compound:5967686|FD-Score:4.57|P-value:7.17E-5 ID:SGTC_2511|Compound:berberine|FD-Score:-3.82|P-value:7.36E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-4.27|P-value:1.84E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:7.48|P-value:2.31E-10 ID:SGTC_2619|Compound:obliquin|FD-Score:5.04|P-value:1.37E-5 ID:SGTC_2739|Compound:tacrine|FD-Score:-4.11|P-value:3.11E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:6.92|P-value:4.21E-9 ID:SGTC_2756|Compound:carbadox|FD-Score:-4.63|P-value:5.77E-5 ID:SGTC_2800|Compound:7887935|FD-Score:3.86|P-value:6.62E-4 ID:SGTC_2912|Compound:7964298|FD-Score:11.00|P-value:3.57E-20 ID:SGTC_3000|Compound:9075062|FD-Score:-4.70|P-value:4.43E-5 ID:SGTC_3009|Compound:9080819|FD-Score:4.17|P-value:2.59E-4 ID:SGTC_3037|Compound:9091026|FD-Score:5.86|P-value:5.43E-7 ID:SGTC_3097|Compound:9117246|FD-Score:4.73|P-value:4.13E-5 ID:SGTC_3138|Compound:9097216|FD-Score:4.05|P-value:3.74E-4 ID:SGTC_3151|Compound:9099821|FD-Score:-3.71|P-value:9.90E-4 ID:SGTC_3203|Compound:9113543|FD-Score:5.22|P-value:6.88E-6 ID:SGTC_3221|Compound:9128209|FD-Score:4.64|P-value:5.65E-5 ID:SGTC_3281|Compound:9140613|FD-Score:4.75|P-value:3.78E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:10.70|P-value:3.45E-19 ID:SGTC_138|Compound:0850-0259|FD-Score:6.83|P-value:6.50E-9 ID:SGTC_163|Compound:1327-0036|FD-Score:-4.70|P-value:4.49E-5 ID:SGTC_197|Compound:4544-0043|FD-Score:4.77|P-value:3.56E-5 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.96|P-value:1.84E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:4.06|P-value:3.68E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:3.77|P-value:8.56E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:6.71|P-value:1.18E-8 ID:SGTC_466|Compound:geldanamycin|FD-Score:5.76|P-value:8.01E-7 ID:SGTC_550|Compound:4123-0385|FD-Score:4.52|P-value:8.36E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:4.95|P-value:1.88E-5 ID:SGTC_618|Compound:k072-0232|FD-Score:-3.83|P-value:7.04E-4 ID:SGTC_657|Compound:3534-0529|FD-Score:3.72|P-value:9.70E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:4.18|P-value:2.50E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:4.61|P-value:6.17E-5 ID:SGTC_908|Compound:3448-7491|FD-Score:-4.60|P-value:6.33E-5 ID:SGTC_909|Compound:3448-9333|FD-Score:-3.83|P-value:7.03E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:3.81|P-value:7.61E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:8.91|P-value:5.82E-14 ID:SGTC_923|Compound:1900-2434|FD-Score:-3.77|P-value:8.32E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:4.17|P-value:2.57E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:3.71|P-value:9.99E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:-3.88|P-value:6.12E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.48|P-value:9.75E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:4.27|P-value:1.91E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:4.16|P-value:2.69E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:4.18|P-value:2.54E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:4.54|P-value:7.79E-5 ID:SGTC_1510|Compound:1123-0200|FD-Score:4.40|P-value:1.23E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:4.10|P-value:3.20E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:8.03|P-value:1.16E-11 ID:SGTC_1605|Compound:st000082|FD-Score:-4.57|P-value:7.12E-5 ID:SGTC_1672|Compound:st016598|FD-Score:5.43|P-value:3.04E-6 ID:SGTC_1700|Compound:st030840|FD-Score:4.74|P-value:3.91E-5 ID:SGTC_1754|Compound:st042065|FD-Score:5.21|P-value:7.11E-6 ID:SGTC_1800|Compound:st050079|FD-Score:8.06|P-value:9.61E-12 ID:SGTC_1803|Compound:st050068|FD-Score:4.90|P-value:2.22E-5 ID:SGTC_1818|Compound:st052136|FD-Score:6.76|P-value:9.12E-9 ID:SGTC_1827|Compound:berberine|FD-Score:-5.67|P-value:1.16E-6 ID:SGTC_1829|Compound:berberine|FD-Score:-8.28|P-value:2.72E-12 ID:SGTC_1836|Compound:st055642|FD-Score:5.60|P-value:1.57E-6 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.55|P-value:7.64E-5 ID:SGTC_1845|Compound:st056753|FD-Score:4.16|P-value:2.64E-4 ID:SGTC_1959|Compound:st077091|FD-Score:4.34|P-value:1.51E-4 ID:SGTC_1987|Compound:st072290|FD-Score:4.92|P-value:2.07E-5 ID:SGTC_2090|Compound:5301565|FD-Score:-3.79|P-value:7.95E-4 ID:SGTC_2096|Compound:5468907|FD-Score:3.92|P-value:5.43E-4 ID:SGTC_2100|Compound:5377183|FD-Score:-3.80|P-value:7.64E-4 ID:SGTC_2101|Compound:5377231|FD-Score:4.42|P-value:1.17E-4 ID:SGTC_2321|Compound:7390090|FD-Score:5.83|P-value:6.03E-7 ID:SGTC_2322|Compound:7413319|FD-Score:4.16|P-value:2.68E-4 ID:SGTC_2328|Compound:6201408|FD-Score:-3.82|P-value:7.27E-4 ID:SGTC_2445|Compound:5742597|FD-Score:3.92|P-value:5.59E-4 ID:SGTC_2477|Compound:5967686|FD-Score:4.57|P-value:7.17E-5 ID:SGTC_2511|Compound:berberine|FD-Score:-3.82|P-value:7.36E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-4.27|P-value:1.84E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:7.48|P-value:2.31E-10 ID:SGTC_2619|Compound:obliquin|FD-Score:5.04|P-value:1.37E-5 ID:SGTC_2739|Compound:tacrine|FD-Score:-4.11|P-value:3.11E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:6.92|P-value:4.21E-9 ID:SGTC_2756|Compound:carbadox|FD-Score:-4.63|P-value:5.77E-5 ID:SGTC_2800|Compound:7887935|FD-Score:3.86|P-value:6.62E-4 ID:SGTC_2912|Compound:7964298|FD-Score:11.00|P-value:3.57E-20 ID:SGTC_3000|Compound:9075062|FD-Score:-4.70|P-value:4.43E-5 ID:SGTC_3009|Compound:9080819|FD-Score:4.17|P-value:2.59E-4 ID:SGTC_3037|Compound:9091026|FD-Score:5.86|P-value:5.43E-7 ID:SGTC_3097|Compound:9117246|FD-Score:4.73|P-value:4.13E-5 ID:SGTC_3138|Compound:9097216|FD-Score:4.05|P-value:3.74E-4 ID:SGTC_3151|Compound:9099821|FD-Score:-3.71|P-value:9.90E-4 ID:SGTC_3203|Compound:9113543|FD-Score:5.22|P-value:6.88E-6 ID:SGTC_3221|Compound:9128209|FD-Score:4.64|P-value:5.65E-5 ID:SGTC_3281|Compound:9140613|FD-Score:4.75|P-value:3.78E-5

Top fitness defect scores for YKL145W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2912 7964298 11.00 10.9 µM 3.57E-20 cell wall 11 3.01E-28 0.41
2 SGTC_58 0957-0393 10.70 9.0 µM 3.45E-19 cell wall 8 7.83E-27 0.55
3 SGTC_916 4466-0038 8.91 42.0 nM 5.82E-14 cell wall 9 2.51E-19 1.72
4 SGTC_1800 st050079 8.06 29.1 µM 9.61E-12 NEO1-PIK1 5 3.79E-16 1.51
5 SGTC_1597 7-benzyloxygramine 8.03 47.5 µM 1.16E-11 calcium & mitochondrial duress 22 4.96E-16 0.30
6 SGTC_2584 androsterone 7.48 48.7 µM 2.31E-10 cell wall 5 3.59E-14 0.26
7 SGTC_2753 fluvastatin 6.92 17.1 µM 4.21E-9 azole & statin 1 2.30E-12 1.24
8 SGTC_138 0850-0259 6.83 40.9 µM 6.50E-9 cell wall 24 4.27E-12 0.11
9 SGTC_1818 st052136 6.76 56.3 µM 9.12E-9 NEO1-PIK1 5 6.93E-12 0.15
10 SGTC_445 e6 berbamine 6.71 66.0 µM 1.18E-8 NEO1-PIK1 12 1.00E-11 0.38
11 SGTC_3037 9091026 5.86 49.5 µM 5.43E-7 NEO1-PIK1 12 2.37E-9 0.25
12 SGTC_2321 7390090 5.83 138.8 µM 6.03E-7 2 2.76E-9 1.61
13 SGTC_466 geldanamycin 5.76 89.2 µM 8.01E-7 RPP1 & pyrimidine depletion 2 4.13E-9 2.09
14 SGTC_1836 st055642 5.60 81.0 µM 1.57E-6 1 1.07E-8 1.11
15 SGTC_1672 st016598 5.43 6.5 µM 3.04E-6 endomembrane recycling 7 2.76E-8 0.07
16 SGTC_3203 9113543 5.22 49.5 µM 6.88E-6 3 8.84E-8 0.19
17 SGTC_1754 st042065 5.21 16.1 µM 7.11E-6 NEO1-PIK1 17 9.27E-8 0.07
18 SGTC_2619 obliquin 5.04 87.9 µM 1.37E-5 cell wall 24 2.35E-7 0.03
19 SGTC_221 trifluoperazine 4.96 10.3 µM 1.84E-5 NEO1-PIK1 21 3.59E-7 0.14
20 SGTC_586 0957-0265 4.95 8.2 µM 1.88E-5 cell wall 29 3.69E-7 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.310 1.31E-75 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.295 1.84E-68 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.286 4.50E-64 YBL061C SKT5 hom Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
0.252 9.86E-50 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.251 2.01E-49 YNL233W BNI4 hom Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p
0.251 2.67E-49 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.244 8.90E-47 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.238 1.54E-44 YPL066W RGL1 hom Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
0.234 4.37E-43 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.226 2.78E-40 YPL115C BEM3 hom Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.223 6.74E-39 YJL081C ARP4 het Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.222 7.96E-39 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.210 7.10E-35 YDR389W SAC7 hom GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate
0.210 7.22E-35 YOR236W DFR1 het Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
0.210 9.98E-35 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C