YKL146W / AVT3

Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL146W / AVT3

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:5.47|P-value:3.09E-8 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.55|P-value:2.28E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:-6.71|P-value:1.71E-11 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.49|P-value:2.87E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:3.22|P-value:7.08E-4 ID:SGTC_235|Compound:0330-0087|FD-Score:3.38|P-value:4.08E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:3.20|P-value:7.63E-4 ID:SGTC_654|Compound:1124-0271|FD-Score:4.79|P-value:1.05E-6 ID:SGTC_742|Compound:0kpi-0133|FD-Score:3.29|P-value:5.53E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:3.31|P-value:5.19E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:3.15|P-value:8.99E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:4.23|P-value:1.39E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:-3.29|P-value:5.81E-4 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.17|P-value:8.95E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:3.42|P-value:3.48E-4 ID:SGTC_1035|Compound:k081-0041|FD-Score:6.09|P-value:8.47E-10 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.64|P-value:1.54E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.30|P-value:5.49E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:3.13|P-value:9.77E-4 ID:SGTC_1782|Compound:st039423|FD-Score:3.27|P-value:6.07E-4 ID:SGTC_1887|Compound:st057648|FD-Score:3.34|P-value:4.68E-4 ID:SGTC_2170|Compound:morpholine|FD-Score:3.84|P-value:7.11E-5 ID:SGTC_2175|Compound:5807670|FD-Score:-3.24|P-value:6.96E-4 ID:SGTC_2181|Compound:6316240|FD-Score:3.16|P-value:8.67E-4 ID:SGTC_2278|Compound:7948257|FD-Score:-3.36|P-value:4.60E-4 ID:SGTC_2624|Compound:nerol|FD-Score:6.27|P-value:2.83E-10 ID:SGTC_2769|Compound:toremifene|FD-Score:4.38|P-value:7.29E-6 ID:SGTC_2773|Compound:thioridazine|FD-Score:3.17|P-value:8.51E-4 ID:SGTC_2792|Compound:7535235|FD-Score:3.90|P-value:5.59E-5 ID:SGTC_2795|Compound:7780010|FD-Score:3.52|P-value:2.43E-4 ID:SGTC_2820|Compound:7989630|FD-Score:-3.59|P-value:1.98E-4 ID:SGTC_2966|Compound:9084041|FD-Score:8.63|P-value:6.75E-18 ID:SGTC_2970|Compound:9090938|FD-Score:-3.21|P-value:7.62E-4 ID:SGTC_3089|Compound:9117425|FD-Score:3.74|P-value:1.08E-4 ID:SGTC_3207|Compound:9114163|FD-Score:3.16|P-value:8.60E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:5.47|P-value:3.09E-8 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.55|P-value:2.28E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:-6.71|P-value:1.71E-11 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.49|P-value:2.87E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:3.22|P-value:7.08E-4 ID:SGTC_235|Compound:0330-0087|FD-Score:3.38|P-value:4.08E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:3.20|P-value:7.63E-4 ID:SGTC_654|Compound:1124-0271|FD-Score:4.79|P-value:1.05E-6 ID:SGTC_742|Compound:0kpi-0133|FD-Score:3.29|P-value:5.53E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:3.31|P-value:5.19E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:3.15|P-value:8.99E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:4.23|P-value:1.39E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:-3.29|P-value:5.81E-4 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.17|P-value:8.95E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:3.42|P-value:3.48E-4 ID:SGTC_1035|Compound:k081-0041|FD-Score:6.09|P-value:8.47E-10 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.64|P-value:1.54E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.30|P-value:5.49E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:3.13|P-value:9.77E-4 ID:SGTC_1782|Compound:st039423|FD-Score:3.27|P-value:6.07E-4 ID:SGTC_1887|Compound:st057648|FD-Score:3.34|P-value:4.68E-4 ID:SGTC_2170|Compound:morpholine|FD-Score:3.84|P-value:7.11E-5 ID:SGTC_2175|Compound:5807670|FD-Score:-3.24|P-value:6.96E-4 ID:SGTC_2181|Compound:6316240|FD-Score:3.16|P-value:8.67E-4 ID:SGTC_2278|Compound:7948257|FD-Score:-3.36|P-value:4.60E-4 ID:SGTC_2624|Compound:nerol|FD-Score:6.27|P-value:2.83E-10 ID:SGTC_2769|Compound:toremifene|FD-Score:4.38|P-value:7.29E-6 ID:SGTC_2773|Compound:thioridazine|FD-Score:3.17|P-value:8.51E-4 ID:SGTC_2792|Compound:7535235|FD-Score:3.90|P-value:5.59E-5 ID:SGTC_2795|Compound:7780010|FD-Score:3.52|P-value:2.43E-4 ID:SGTC_2820|Compound:7989630|FD-Score:-3.59|P-value:1.98E-4 ID:SGTC_2966|Compound:9084041|FD-Score:8.63|P-value:6.75E-18 ID:SGTC_2970|Compound:9090938|FD-Score:-3.21|P-value:7.62E-4 ID:SGTC_3089|Compound:9117425|FD-Score:3.74|P-value:1.08E-4 ID:SGTC_3207|Compound:9114163|FD-Score:3.16|P-value:8.60E-4

Top fitness defect scores for YKL146W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2966 9084041 8.63 48.0 µM 6.75E-18 2 3.02E-18
2 SGTC_2624 nerol 6.27 100.0 µM 2.83E-10 1 1.86E-10
3 SGTC_1035 k081-0041 6.09 55.2 µM 8.47E-10 Golgi 5 5.71E-10
4 SGTC_6 0844-0013 5.47 19.8 µM 3.09E-8 23 2.25E-8
5 SGTC_654 1124-0271 4.79 80.9 µM 1.05E-6 8 8.26E-7
6 SGTC_2769 toremifene 4.38 3.7 µM 7.29E-6 15 5.96E-6
7 SGTC_931 2812-0076 4.23 566.0 µM 1.39E-5 17 1.16E-5
8 SGTC_2792 7535235 3.90 81.8 µM 5.59E-5 fatty acid desaturase (OLE1) 32 4.77E-5
9 SGTC_2170 morpholine 3.84 200.0 µM 7.11E-5 12 6.10E-5
10 SGTC_3089 9117425 3.74 49.5 µM 1.08E-4 31 9.35E-5
11 SGTC_1039 k821-0043 3.64 94.0 µM 1.54E-4 Golgi 38 1.34E-4
12 SGTC_2795 7780010 3.52 54.5 µM 2.43E-4 50 2.14E-4
13 SGTC_1025 k007-0731 3.42 70.0 µM 3.48E-4 30 3.08E-4
14 SGTC_235 0330-0087 3.38 5.0 mM 4.08E-4 TSC3-RPN4 46 3.63E-4
15 SGTC_1887 st057648 3.34 70.8 µM 4.68E-4 32 4.17E-4
16 SGTC_781 4335-2806 3.31 94.4 µM 5.19E-4 41 4.64E-4
17 SGTC_1045 1348-1485 3.30 165.0 µM 5.49E-4 65 4.91E-4
18 SGTC_742 0kpi-0133 3.29 140.0 µM 5.53E-4 ERG2 40 4.95E-4
19 SGTC_1782 st039423 3.27 80.6 µM 6.07E-4 49 5.44E-4
20 SGTC_154 3389-1638 3.22 511.1 µM 7.08E-4 51 6.36E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.127 1.68E-13 YHR085W IPI1 het Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene
0.121 1.65E-12 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.113 4.51E-11 YKR053C YSR3 hom Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication
0.113 5.09E-11 YDR453C TSA2 hom Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress
0.112 8.74E-11 YGR198W YPP1 het Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
0.106 8.57E-10 YKR069W MET1 hom S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis
0.103 2.15E-9 YLR319C BUD6 hom Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate
0.103 2.23E-9 YPL192C PRM3 hom Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body
0.100 6.46E-9 YKL164C PIR1 hom O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication
0.097 2.15E-8 YKL187C FAT3 hom Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies
0.095 3.02E-8 YPR151C SUE1 hom Mitochondrial protein required for degradation of unstable forms of cytochrome c
0.094 4.31E-8 YKR043C SHB17 hom Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus
0.093 6.07E-8 YBR147W RTC2 hom Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter
0.093 7.39E-8 YDR051C DET1 hom Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
0.092 8.99E-8 YDL034W_d YDL034W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene