YKL149C / DBR1

RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL149C / DBR1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:4.83|P-value:7.75E-7 ID:SGTC_71|Compound:0417-1750|FD-Score:4.89|P-value:5.83E-7 ID:SGTC_103|Compound:0844-0104|FD-Score:4.46|P-value:4.62E-6 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:5.00|P-value:3.44E-7 ID:SGTC_257|Compound:daunorubicin|FD-Score:-3.49|P-value:3.81E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.68|P-value:1.85E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.33|P-value:4.41E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:3.18|P-value:7.36E-4 ID:SGTC_512|Compound:prima-1|FD-Score:-4.02|P-value:4.93E-5 ID:SGTC_517|Compound:1365-0151|FD-Score:-4.61|P-value:3.91E-6 ID:SGTC_518|Compound:1021-0119|FD-Score:-5.35|P-value:9.89E-8 ID:SGTC_522|Compound:1273-0010|FD-Score:5.41|P-value:4.01E-8 ID:SGTC_838|Compound:0109-0203|FD-Score:3.58|P-value:1.74E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:4.37|P-value:6.79E-6 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.96|P-value:4.00E-5 ID:SGTC_1318|Compound:1280-3875|FD-Score:3.31|P-value:4.78E-4 ID:SGTC_1498|Compound:4478-3555|FD-Score:3.49|P-value:2.44E-4 ID:SGTC_1728|Compound:st035614|FD-Score:3.68|P-value:1.22E-4 ID:SGTC_1800|Compound:st050079|FD-Score:-3.73|P-value:1.56E-4 ID:SGTC_1801|Compound:st050089|FD-Score:-4.38|P-value:1.11E-5 ID:SGTC_1803|Compound:st050068|FD-Score:-4.36|P-value:1.17E-5 ID:SGTC_1818|Compound:st052136|FD-Score:-4.36|P-value:1.18E-5 ID:SGTC_2001|Compound:4023502|FD-Score:4.48|P-value:4.09E-6 ID:SGTC_2035|Compound:5154463|FD-Score:4.74|P-value:1.21E-6 ID:SGTC_2037|Compound:5169046|FD-Score:3.73|P-value:1.01E-4 ID:SGTC_2038|Compound:4100038|FD-Score:-5.10|P-value:3.65E-7 ID:SGTC_2045|Compound:5106652|FD-Score:4.14|P-value:1.91E-5 ID:SGTC_2111|Compound:5486399|FD-Score:-3.94|P-value:6.80E-5 ID:SGTC_2122|Compound:5258151|FD-Score:-4.25|P-value:1.88E-5 ID:SGTC_2172|Compound:5754775|FD-Score:-4.25|P-value:1.90E-5 ID:SGTC_2186|Compound:6124449|FD-Score:-3.51|P-value:3.48E-4 ID:SGTC_2465|Compound:5809242|FD-Score:3.33|P-value:4.43E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:6.63|P-value:2.55E-11 ID:SGTC_2512|Compound:diallyl trisulfide|FD-Score:3.82|P-value:6.88E-5 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:3.38|P-value:3.66E-4 ID:SGTC_2800|Compound:7887935|FD-Score:4.30|P-value:9.37E-6 ID:SGTC_2956|Compound:9076196|FD-Score:3.97|P-value:3.76E-5 ID:SGTC_2961|Compound:9083290|FD-Score:3.12|P-value:9.12E-4 ID:SGTC_3022|Compound:9087993|FD-Score:3.12|P-value:8.90E-4 ID:SGTC_3166|Compound:9102297|FD-Score:-3.51|P-value:3.49E-4 ID:SGTC_3169|Compound:9102970|FD-Score:3.59|P-value:1.71E-4 ID:SGTC_3184|Compound:9108758|FD-Score:5.04|P-value:2.75E-7 ID:SGTC_6|Compound:0844-0013|FD-Score:4.83|P-value:7.75E-7 ID:SGTC_71|Compound:0417-1750|FD-Score:4.89|P-value:5.83E-7 ID:SGTC_103|Compound:0844-0104|FD-Score:4.46|P-value:4.62E-6 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:5.00|P-value:3.44E-7 ID:SGTC_257|Compound:daunorubicin|FD-Score:-3.49|P-value:3.81E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.68|P-value:1.85E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.33|P-value:4.41E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:3.18|P-value:7.36E-4 ID:SGTC_512|Compound:prima-1|FD-Score:-4.02|P-value:4.93E-5 ID:SGTC_517|Compound:1365-0151|FD-Score:-4.61|P-value:3.91E-6 ID:SGTC_518|Compound:1021-0119|FD-Score:-5.35|P-value:9.89E-8 ID:SGTC_522|Compound:1273-0010|FD-Score:5.41|P-value:4.01E-8 ID:SGTC_838|Compound:0109-0203|FD-Score:3.58|P-value:1.74E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:4.37|P-value:6.79E-6 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.96|P-value:4.00E-5 ID:SGTC_1318|Compound:1280-3875|FD-Score:3.31|P-value:4.78E-4 ID:SGTC_1498|Compound:4478-3555|FD-Score:3.49|P-value:2.44E-4 ID:SGTC_1728|Compound:st035614|FD-Score:3.68|P-value:1.22E-4 ID:SGTC_1800|Compound:st050079|FD-Score:-3.73|P-value:1.56E-4 ID:SGTC_1801|Compound:st050089|FD-Score:-4.38|P-value:1.11E-5 ID:SGTC_1803|Compound:st050068|FD-Score:-4.36|P-value:1.17E-5 ID:SGTC_1818|Compound:st052136|FD-Score:-4.36|P-value:1.18E-5 ID:SGTC_2001|Compound:4023502|FD-Score:4.48|P-value:4.09E-6 ID:SGTC_2035|Compound:5154463|FD-Score:4.74|P-value:1.21E-6 ID:SGTC_2037|Compound:5169046|FD-Score:3.73|P-value:1.01E-4 ID:SGTC_2038|Compound:4100038|FD-Score:-5.10|P-value:3.65E-7 ID:SGTC_2045|Compound:5106652|FD-Score:4.14|P-value:1.91E-5 ID:SGTC_2111|Compound:5486399|FD-Score:-3.94|P-value:6.80E-5 ID:SGTC_2122|Compound:5258151|FD-Score:-4.25|P-value:1.88E-5 ID:SGTC_2172|Compound:5754775|FD-Score:-4.25|P-value:1.90E-5 ID:SGTC_2186|Compound:6124449|FD-Score:-3.51|P-value:3.48E-4 ID:SGTC_2465|Compound:5809242|FD-Score:3.33|P-value:4.43E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:6.63|P-value:2.55E-11 ID:SGTC_2512|Compound:diallyl trisulfide|FD-Score:3.82|P-value:6.88E-5 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:3.38|P-value:3.66E-4 ID:SGTC_2800|Compound:7887935|FD-Score:4.30|P-value:9.37E-6 ID:SGTC_2956|Compound:9076196|FD-Score:3.97|P-value:3.76E-5 ID:SGTC_2961|Compound:9083290|FD-Score:3.12|P-value:9.12E-4 ID:SGTC_3022|Compound:9087993|FD-Score:3.12|P-value:8.90E-4 ID:SGTC_3166|Compound:9102297|FD-Score:-3.51|P-value:3.49E-4 ID:SGTC_3169|Compound:9102970|FD-Score:3.59|P-value:1.71E-4 ID:SGTC_3184|Compound:9108758|FD-Score:5.04|P-value:2.75E-7

Top fitness defect scores for YKL149C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 6.63 3.7 µM 2.55E-11 8 1.68E-11
2 SGTC_522 1273-0010 5.41 11.6 µM 4.01E-8 60S ribosome export 7 3.21E-8
3 SGTC_3184 9108758 5.04 49.5 µM 2.75E-7 1 2.31E-7
4 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 5.00 4.6 µM 3.44E-7 heat shock/prefoldin 22 2.90E-7
5 SGTC_71 0417-1750 4.89 47.8 µM 5.83E-7 Golgi 27 4.98E-7
6 SGTC_6 0844-0013 4.83 19.8 µM 7.75E-7 43 6.68E-7
7 SGTC_2035 5154463 4.74 94.6 µM 1.21E-6 6 1.06E-6
8 SGTC_2001 4023502 4.48 57.7 µM 4.09E-6 mitochondrial processes 8 3.66E-6
9 SGTC_103 0844-0104 4.46 51.8 µM 4.62E-6 ERAD & cell cycle 122 4.15E-6
10 SGTC_978 1416-0512 4.37 87.4 µM 6.79E-6 redox potentiating 24 6.15E-6
11 SGTC_2800 7887935 4.30 81.8 µM 9.37E-6 RPP1 & pyrimidine depletion 22 8.55E-6
12 SGTC_2045 5106652 4.14 78.0 µM 1.91E-5 11 1.77E-5
13 SGTC_2956 9076196 3.97 2.5 µM 3.76E-5 15 3.54E-5
14 SGTC_1298 1079-1278 3.96 263.0 µM 4.00E-5 DNA damage response 16 3.77E-5
15 SGTC_2512 diallyl trisulfide 3.82 49.2 µM 6.88E-5 10 6.56E-5
16 SGTC_2037 5169046 3.73 200.0 µM 1.01E-4 60S ribosome export 16 9.67E-5
17 SGTC_1728 st035614 3.68 33.8 µM 1.22E-4 65 1.18E-4
18 SGTC_3169 9102970 3.59 49.5 µM 1.71E-4 RPP1 & pyrimidine depletion 9 1.66E-4
19 SGTC_838 0109-0203 3.58 71.4 µM 1.74E-4 26 1.69E-4
20 SGTC_1498 4478-3555 3.49 97.6 µM 2.44E-4 10 2.38E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.154 2.47E-19 YPL004C LSP1 hom Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family
0.147 1.22E-17 YDR374C_p YDR374C_p hom Putative protein of unknown function
0.142 1.22E-16 YDR179C CSN9 hom Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling
0.134 6.72E-15 YNL218W MGS1 hom Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
0.124 5.44E-13 YHR021W-A_p ECM12_p hom Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.121 1.77E-12 YGR242W_d YGR242W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C
0.119 4.81E-12 YNL242W ATG2 hom Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.118 5.84E-12 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.116 1.69E-11 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.111 1.11E-10 YKL172W EBP2 het Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.110 1.74E-10 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.110 1.88E-10 YKL201C MNN4 hom Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication
0.109 2.15E-10 YKL161C KDX1 hom Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p
0.109 2.65E-10 YMR080C NAM7 hom ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
0.108 3.51E-10 YKR009C FOX2 hom Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities