YKL150W / MCR1

Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL150W / MCR1

Click on Significant Values for Screen Details ID:SGTC_77|Compound:0828-0286|FD-Score:3.50|P-value:7.35E-4 ID:SGTC_398|Compound:bronopol|FD-Score:-3.91|P-value:1.25E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:-3.95|P-value:1.11E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:4.03|P-value:1.22E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:5.52|P-value:2.26E-7 ID:SGTC_1016|Compound:4226-1401|FD-Score:4.20|P-value:6.50E-5 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.55|P-value:6.22E-4 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:-3.46|P-value:5.97E-4 ID:SGTC_1295|Compound:1013-0063|FD-Score:3.43|P-value:9.11E-4 ID:SGTC_1444|Compound:4112-3581|FD-Score:3.76|P-value:3.16E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:3.44|P-value:8.74E-4 ID:SGTC_1913|Compound:st060213|FD-Score:-5.80|P-value:3.21E-8 ID:SGTC_2278|Compound:7948257|FD-Score:-3.64|P-value:3.30E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.43|P-value:9.10E-4 ID:SGTC_2296|Compound:7971656|FD-Score:-3.63|P-value:3.33E-4 ID:SGTC_2415|Compound:st077333|FD-Score:-4.10|P-value:6.20E-5 ID:SGTC_2434|Compound:5670830|FD-Score:3.56|P-value:5.96E-4 ID:SGTC_2484|Compound:5809818|FD-Score:5.20|P-value:1.02E-6 ID:SGTC_2803|Compound:7707263|FD-Score:4.96|P-value:2.93E-6 ID:SGTC_2890|Compound:9046729|FD-Score:-3.47|P-value:5.63E-4 ID:SGTC_2956|Compound:9076196|FD-Score:4.36|P-value:3.57E-5 ID:SGTC_2967|Compound:9089538|FD-Score:-4.00|P-value:9.13E-5 ID:SGTC_2968|Compound:9090663|FD-Score:-4.02|P-value:8.43E-5 ID:SGTC_3212|Compound:9128307|FD-Score:3.63|P-value:4.81E-4 ID:SGTC_3243|Compound:9134147|FD-Score:3.41|P-value:9.73E-4 ID:SGTC_3300|Compound:9124615|FD-Score:-5.23|P-value:5.30E-7 ID:SGTC_77|Compound:0828-0286|FD-Score:3.50|P-value:7.35E-4 ID:SGTC_398|Compound:bronopol|FD-Score:-3.91|P-value:1.25E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:-3.95|P-value:1.11E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:4.03|P-value:1.22E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:5.52|P-value:2.26E-7 ID:SGTC_1016|Compound:4226-1401|FD-Score:4.20|P-value:6.50E-5 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.55|P-value:6.22E-4 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:-3.46|P-value:5.97E-4 ID:SGTC_1295|Compound:1013-0063|FD-Score:3.43|P-value:9.11E-4 ID:SGTC_1444|Compound:4112-3581|FD-Score:3.76|P-value:3.16E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:3.44|P-value:8.74E-4 ID:SGTC_1913|Compound:st060213|FD-Score:-5.80|P-value:3.21E-8 ID:SGTC_2278|Compound:7948257|FD-Score:-3.64|P-value:3.30E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.43|P-value:9.10E-4 ID:SGTC_2296|Compound:7971656|FD-Score:-3.63|P-value:3.33E-4 ID:SGTC_2415|Compound:st077333|FD-Score:-4.10|P-value:6.20E-5 ID:SGTC_2434|Compound:5670830|FD-Score:3.56|P-value:5.96E-4 ID:SGTC_2484|Compound:5809818|FD-Score:5.20|P-value:1.02E-6 ID:SGTC_2803|Compound:7707263|FD-Score:4.96|P-value:2.93E-6 ID:SGTC_2890|Compound:9046729|FD-Score:-3.47|P-value:5.63E-4 ID:SGTC_2956|Compound:9076196|FD-Score:4.36|P-value:3.57E-5 ID:SGTC_2967|Compound:9089538|FD-Score:-4.00|P-value:9.13E-5 ID:SGTC_2968|Compound:9090663|FD-Score:-4.02|P-value:8.43E-5 ID:SGTC_3212|Compound:9128307|FD-Score:3.63|P-value:4.81E-4 ID:SGTC_3243|Compound:9134147|FD-Score:3.41|P-value:9.73E-4 ID:SGTC_3300|Compound:9124615|FD-Score:-5.23|P-value:5.30E-7

Top fitness defect scores for YKL150W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_902 1120-0002 5.52 2.1 µM 2.26E-7 azole & statin 22 1.69E-8
2 SGTC_2484 5809818 5.20 12.0 µM 1.02E-6 6 1.00E-7
3 SGTC_2803 7707263 4.96 71.4 µM 2.93E-6 16 3.47E-7
4 SGTC_2956 9076196 4.36 2.5 µM 3.57E-5 9 6.61E-6
5 SGTC_1016 4226-1401 4.20 38.0 µM 6.50E-5 RNA pol III & RNase P/MRP 14 1.34E-5
6 SGTC_747 3448-8087 4.03 38.7 µM 1.22E-4 23 2.82E-5
7 SGTC_1444 4112-3581 3.76 28.0 µM 3.16E-4 13 8.61E-5
8 SGTC_3212 9128307 3.63 49.5 µM 4.81E-4 62 1.41E-4
9 SGTC_2434 5670830 3.56 169.9 µM 5.96E-4 37 1.82E-4
10 SGTC_1077 5-fluorocytosine 3.55 377.0 nM 6.22E-4 exosome 28 1.92E-4
11 SGTC_77 0828-0286 3.50 12.5 µM 7.35E-4 TSC3-RPN4 57 2.33E-4
12 SGTC_1527 coumarin 3.44 137.0 µM 8.74E-4 39 2.86E-4
13 SGTC_2293 7624732 3.43 200.0 µM 9.10E-4 41 3.00E-4
14 SGTC_1295 1013-0063 3.43 9.8 µM 9.11E-4 28 3.00E-4
15 SGTC_3243 9134147 3.41 49.5 µM 9.73E-4 8 3.25E-4
16 SGTC_1901 st060216 3.32 58.6 µM 0.00127 43 4.45E-4
17 SGTC_2330 7979373 3.30 200.0 µM 0.00138 60S ribosome export 57 4.88E-4
18 SGTC_1573 oleylamine 3.27 1.1 µM 0.00149 60 5.37E-4
19 SGTC_2581 avocatin a 3.24 64.0 µM 0.00166 sphingolipid biosynthesis & PDR1 79 6.07E-4
20 SGTC_423 5-fluorouracil 3.22 457.0 µM 0.00172 exosome 53 6.34E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.160 1.50E-20 YAL062W GDH3 hom NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication
0.116 1.63E-11 YKL172W EBP2 het Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.112 7.79E-11 YPL167C REV3 hom Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
0.110 1.97E-10 YFL028C CAF16 hom Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator
0.096 2.74E-8 YLR187W SKG3 hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication
0.091 1.50E-7 YBR228W SLX1 hom Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p
0.089 2.15E-7 YKL149C DBR1 hom RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition
0.086 5.88E-7 YNL087W TCB2 hom ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud
0.084 1.04E-6 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.084 1.15E-6 YOR354C MSC6 hom Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.083 1.30E-6 YPR142C_d YPR142C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
0.080 3.63E-6 YHL003C LAG1 hom Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress
0.080 3.61E-6 YBL103C RTG3 hom Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways
0.078 6.06E-6 YGR034W RPL26B hom Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication
0.077 7.56E-6 YPR044C_d OPI11_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO