YKL151C

NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL151C / YKL151C

Click on Significant Values for Screen Details ID:SGTC_55|Compound:0767-0285|FD-Score:-2.93|P-value:6.23E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:2.78|P-value:8.78E-4 ID:SGTC_469|Compound:w7|FD-Score:-3.81|P-value:1.27E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.24|P-value:1.80E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:2.96|P-value:4.29E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.52|P-value:3.79E-5 ID:SGTC_1290|Compound:0986-0249|FD-Score:-2.92|P-value:6.46E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:3.24|P-value:1.33E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.01|P-value:4.53E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.00|P-value:3.60E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.53|P-value:3.63E-5 ID:SGTC_1684|Compound:st024538|FD-Score:3.42|P-value:6.08E-5 ID:SGTC_1696|Compound:st025591|FD-Score:2.89|P-value:5.73E-4 ID:SGTC_1728|Compound:st035614|FD-Score:2.96|P-value:4.35E-4 ID:SGTC_1916|Compound:st067606|FD-Score:5.51|P-value:3.31E-10 ID:SGTC_1919|Compound:st067113|FD-Score:5.01|P-value:9.93E-9 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.42|P-value:5.27E-7 ID:SGTC_2053|Compound:5265679|FD-Score:-3.27|P-value:1.54E-4 ID:SGTC_2134|Compound:5324836|FD-Score:3.35|P-value:8.14E-5 ID:SGTC_2135|Compound:5326629|FD-Score:-3.33|P-value:1.18E-4 ID:SGTC_2357|Compound:9067248|FD-Score:-3.62|P-value:3.24E-5 ID:SGTC_2374|Compound:9071832|FD-Score:2.76|P-value:9.29E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.55|P-value:4.45E-5 ID:SGTC_2440|Compound:5628896|FD-Score:3.62|P-value:2.35E-5 ID:SGTC_2532|Compound:xanthone|FD-Score:2.75|P-value:9.72E-4 ID:SGTC_2600|Compound:tangeritin|FD-Score:-3.55|P-value:4.47E-5 ID:SGTC_2620|Compound:irigenin|FD-Score:2.88|P-value:5.97E-4 ID:SGTC_2803|Compound:7707263|FD-Score:2.96|P-value:4.26E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-2.96|P-value:5.53E-4 ID:SGTC_2889|Compound:9059656|FD-Score:2.87|P-value:6.20E-4 ID:SGTC_2939|Compound:9044578|FD-Score:-4.73|P-value:8.44E-8 ID:SGTC_2940|Compound:9060638|FD-Score:-3.19|P-value:2.22E-4 ID:SGTC_2970|Compound:9090938|FD-Score:4.39|P-value:4.19E-7 ID:SGTC_2972|Compound:9093619|FD-Score:-3.44|P-value:7.52E-5 ID:SGTC_2978|Compound:9011784|FD-Score:2.85|P-value:6.74E-4 ID:SGTC_3054|Compound:9094397|FD-Score:-2.92|P-value:6.42E-4 ID:SGTC_3055|Compound:9094636|FD-Score:-2.94|P-value:5.97E-4 ID:SGTC_3191|Compound:9110352|FD-Score:-3.99|P-value:5.25E-6 ID:SGTC_3195|Compound:9113082|FD-Score:4.15|P-value:1.61E-6 ID:SGTC_3202|Compound:9113761|FD-Score:3.79|P-value:1.04E-5 ID:SGTC_3211|Compound:9128781|FD-Score:5.39|P-value:7.82E-10 ID:SGTC_3283|Compound:9109332|FD-Score:-4.64|P-value:1.45E-7 ID:SGTC_55|Compound:0767-0285|FD-Score:-2.93|P-value:6.23E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:2.78|P-value:8.78E-4 ID:SGTC_469|Compound:w7|FD-Score:-3.81|P-value:1.27E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.24|P-value:1.80E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:2.96|P-value:4.29E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.52|P-value:3.79E-5 ID:SGTC_1290|Compound:0986-0249|FD-Score:-2.92|P-value:6.46E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:3.24|P-value:1.33E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.01|P-value:4.53E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.00|P-value:3.60E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.53|P-value:3.63E-5 ID:SGTC_1684|Compound:st024538|FD-Score:3.42|P-value:6.08E-5 ID:SGTC_1696|Compound:st025591|FD-Score:2.89|P-value:5.73E-4 ID:SGTC_1728|Compound:st035614|FD-Score:2.96|P-value:4.35E-4 ID:SGTC_1916|Compound:st067606|FD-Score:5.51|P-value:3.31E-10 ID:SGTC_1919|Compound:st067113|FD-Score:5.01|P-value:9.93E-9 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.42|P-value:5.27E-7 ID:SGTC_2053|Compound:5265679|FD-Score:-3.27|P-value:1.54E-4 ID:SGTC_2134|Compound:5324836|FD-Score:3.35|P-value:8.14E-5 ID:SGTC_2135|Compound:5326629|FD-Score:-3.33|P-value:1.18E-4 ID:SGTC_2357|Compound:9067248|FD-Score:-3.62|P-value:3.24E-5 ID:SGTC_2374|Compound:9071832|FD-Score:2.76|P-value:9.29E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.55|P-value:4.45E-5 ID:SGTC_2440|Compound:5628896|FD-Score:3.62|P-value:2.35E-5 ID:SGTC_2532|Compound:xanthone|FD-Score:2.75|P-value:9.72E-4 ID:SGTC_2600|Compound:tangeritin|FD-Score:-3.55|P-value:4.47E-5 ID:SGTC_2620|Compound:irigenin|FD-Score:2.88|P-value:5.97E-4 ID:SGTC_2803|Compound:7707263|FD-Score:2.96|P-value:4.26E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-2.96|P-value:5.53E-4 ID:SGTC_2889|Compound:9059656|FD-Score:2.87|P-value:6.20E-4 ID:SGTC_2939|Compound:9044578|FD-Score:-4.73|P-value:8.44E-8 ID:SGTC_2940|Compound:9060638|FD-Score:-3.19|P-value:2.22E-4 ID:SGTC_2970|Compound:9090938|FD-Score:4.39|P-value:4.19E-7 ID:SGTC_2972|Compound:9093619|FD-Score:-3.44|P-value:7.52E-5 ID:SGTC_2978|Compound:9011784|FD-Score:2.85|P-value:6.74E-4 ID:SGTC_3054|Compound:9094397|FD-Score:-2.92|P-value:6.42E-4 ID:SGTC_3055|Compound:9094636|FD-Score:-2.94|P-value:5.97E-4 ID:SGTC_3191|Compound:9110352|FD-Score:-3.99|P-value:5.25E-6 ID:SGTC_3195|Compound:9113082|FD-Score:4.15|P-value:1.61E-6 ID:SGTC_3202|Compound:9113761|FD-Score:3.79|P-value:1.04E-5 ID:SGTC_3211|Compound:9128781|FD-Score:5.39|P-value:7.82E-10 ID:SGTC_3283|Compound:9109332|FD-Score:-4.64|P-value:1.45E-7

Top fitness defect scores for YKL151C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1916 st067606 5.51 49.2 µM 3.31E-10 12 1.76E-8
2 SGTC_3211 9128781 5.39 49.5 µM 7.82E-10 26 3.53E-8
3 SGTC_1919 st067113 5.01 52.3 µM 9.93E-9 TSC3-RPN4 15 2.74E-7
4 SGTC_2970 9090938 4.39 41.9 µM 4.19E-7 RNA processing & uracil transport 26 5.62E-6
5 SGTC_3195 9113082 4.15 49.5 µM 1.61E-6 15 1.67E-5
6 SGTC_3202 9113761 3.79 49.5 µM 1.04E-5 52 7.52E-5
7 SGTC_2440 5628896 3.62 157.8 µM 2.35E-5 32 1.46E-4
8 SGTC_1568 digitonin 3.53 820.0 nM 3.63E-5 sphingolipid biosynthesis & PDR1 46 2.07E-4
9 SGTC_1284 0929-0043 3.52 16.0 µM 3.79E-5 37 2.15E-4
10 SGTC_1684 st024538 3.42 14.6 µM 6.08E-5 17 3.15E-4
11 SGTC_2134 5324836 3.35 1.5 µM 8.14E-5 30 3.99E-4
12 SGTC_1291 0993-0025 3.24 5.8 µM 1.33E-4 26 5.95E-4
13 SGTC_1566 6,7-dimethoxy-4-ethylcoumarin 3.00 85.4 µM 3.60E-4 70 0.00133
14 SGTC_2803 7707263 2.96 71.4 µM 4.26E-4 71 0.00153
15 SGTC_1034 k072-0165 2.96 12.7 µM 4.29E-4 Golgi 69 0.00153
16 SGTC_1728 st035614 2.96 33.8 µM 4.35E-4 117 0.00155
17 SGTC_1696 st025591 2.89 34.8 µM 5.73E-4 SWF1 & branched chain AA biosynthesis 79 0.00194
18 SGTC_2620 irigenin 2.88 100.0 µM 5.97E-4 93 0.00200
19 SGTC_2889 9059656 2.87 52.0 µM 6.20E-4 43 0.00207
20 SGTC_2978 9011784 2.85 43.0 µM 6.74E-4 100 0.00221

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.217 6.46E-37 YER069W ARG5,6 hom Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
0.154 2.37E-19 YOR345C_d YOR345C_d hom Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
0.154 3.23E-19 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.148 8.61E-18 YJL068C YJL068C hom Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D
0.145 3.35E-17 YOL140W ARG8 hom Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
0.144 6.24E-17 YER068C-A_d YER068C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.142 1.67E-16 YBL068W PRS4 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication
0.122 1.50E-12 YMR020W FMS1 hom Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis
0.119 4.73E-12 YGR122C-A_d YGR122C-A_d hom Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W
0.119 4.78E-12 YLR118C YLR118C hom Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS
0.118 5.91E-12 YLR178C TFS1 hom Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-binding protein (PEBP) family member; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress
0.118 7.11E-12 YER060W-A FCY22 hom Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
0.118 8.67E-12 YEL004W YEA4 hom Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER
0.113 5.14E-11 YJL211C_d YJL211C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2
0.113 5.90E-11 YKL117W SBA1 hom Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress