YKL156W / RPS27A

Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL156W / RPS27A

Click on Significant Values for Screen Details ID:SGTC_260|Compound:nsc-65238|FD-Score:4.09|P-value:4.27E-5 ID:SGTC_365|Compound:0121-0052|FD-Score:5.07|P-value:5.33E-7 ID:SGTC_430|Compound:0958-0115|FD-Score:3.23|P-value:9.61E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-3.70|P-value:1.67E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:3.62|P-value:2.48E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:6.04|P-value:3.14E-9 ID:SGTC_518|Compound:1021-0119|FD-Score:-5.11|P-value:3.88E-7 ID:SGTC_519|Compound:k048-0011|FD-Score:-3.80|P-value:1.17E-4 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.63|P-value:2.45E-4 ID:SGTC_1080|Compound:nisoldipine|FD-Score:3.69|P-value:1.92E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.26|P-value:8.74E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:5.85|P-value:8.88E-9 ID:SGTC_1259|Compound:0671-0132|FD-Score:4.09|P-value:4.16E-5 ID:SGTC_1270|Compound:0831-1170|FD-Score:-3.32|P-value:6.56E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:4.58|P-value:5.22E-6 ID:SGTC_1773|Compound:st048542|FD-Score:-3.40|P-value:4.93E-4 ID:SGTC_1776|Compound:st049249|FD-Score:-3.59|P-value:2.55E-4 ID:SGTC_1797|Compound:st048695|FD-Score:3.69|P-value:1.95E-4 ID:SGTC_1833|Compound:st055984|FD-Score:4.00|P-value:6.12E-5 ID:SGTC_1842|Compound:st055362|FD-Score:4.36|P-value:1.38E-5 ID:SGTC_2019|Compound:4016004|FD-Score:4.05|P-value:5.04E-5 ID:SGTC_2329|Compound:6138490|FD-Score:-6.41|P-value:2.86E-10 ID:SGTC_2416|Compound:tofa|FD-Score:3.65|P-value:2.28E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:3.81|P-value:1.25E-4 ID:SGTC_2558|Compound:carylophyllene oxide|FD-Score:3.35|P-value:6.51E-4 ID:SGTC_2561|Compound:acetosyringone|FD-Score:4.00|P-value:6.08E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.32|P-value:1.66E-5 ID:SGTC_2693|Compound:st077581|FD-Score:-4.73|P-value:2.32E-6 ID:SGTC_2993|Compound:9054257|FD-Score:3.59|P-value:2.83E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-3.25|P-value:8.22E-4 ID:SGTC_3208|Compound:9114115|FD-Score:-3.69|P-value:1.76E-4 ID:SGTC_3211|Compound:9128781|FD-Score:4.23|P-value:2.42E-5 ID:SGTC_260|Compound:nsc-65238|FD-Score:4.09|P-value:4.27E-5 ID:SGTC_365|Compound:0121-0052|FD-Score:5.07|P-value:5.33E-7 ID:SGTC_430|Compound:0958-0115|FD-Score:3.23|P-value:9.61E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-3.70|P-value:1.67E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:3.62|P-value:2.48E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:6.04|P-value:3.14E-9 ID:SGTC_518|Compound:1021-0119|FD-Score:-5.11|P-value:3.88E-7 ID:SGTC_519|Compound:k048-0011|FD-Score:-3.80|P-value:1.17E-4 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.63|P-value:2.45E-4 ID:SGTC_1080|Compound:nisoldipine|FD-Score:3.69|P-value:1.92E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.26|P-value:8.74E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:5.85|P-value:8.88E-9 ID:SGTC_1259|Compound:0671-0132|FD-Score:4.09|P-value:4.16E-5 ID:SGTC_1270|Compound:0831-1170|FD-Score:-3.32|P-value:6.56E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:4.58|P-value:5.22E-6 ID:SGTC_1773|Compound:st048542|FD-Score:-3.40|P-value:4.93E-4 ID:SGTC_1776|Compound:st049249|FD-Score:-3.59|P-value:2.55E-4 ID:SGTC_1797|Compound:st048695|FD-Score:3.69|P-value:1.95E-4 ID:SGTC_1833|Compound:st055984|FD-Score:4.00|P-value:6.12E-5 ID:SGTC_1842|Compound:st055362|FD-Score:4.36|P-value:1.38E-5 ID:SGTC_2019|Compound:4016004|FD-Score:4.05|P-value:5.04E-5 ID:SGTC_2329|Compound:6138490|FD-Score:-6.41|P-value:2.86E-10 ID:SGTC_2416|Compound:tofa|FD-Score:3.65|P-value:2.28E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:3.81|P-value:1.25E-4 ID:SGTC_2558|Compound:carylophyllene oxide|FD-Score:3.35|P-value:6.51E-4 ID:SGTC_2561|Compound:acetosyringone|FD-Score:4.00|P-value:6.08E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.32|P-value:1.66E-5 ID:SGTC_2693|Compound:st077581|FD-Score:-4.73|P-value:2.32E-6 ID:SGTC_2993|Compound:9054257|FD-Score:3.59|P-value:2.83E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-3.25|P-value:8.22E-4 ID:SGTC_3208|Compound:9114115|FD-Score:-3.69|P-value:1.76E-4 ID:SGTC_3211|Compound:9128781|FD-Score:4.23|P-value:2.42E-5

Top fitness defect scores for YKL156W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_517 1365-0151 6.04 113.0 µM 3.14E-9 10 7.87E-10
2 SGTC_1087 pterostilbene 5.85 9.1 µM 8.88E-9 5 2.41E-9
3 SGTC_365 0121-0052 5.07 13.6 µM 5.33E-7 mitochondrial response to ROS 31 1.97E-7
4 SGTC_1369 1866-0067 4.58 208.0 µM 5.22E-6 RPP1 & pyrimidine depletion 23 2.28E-6
5 SGTC_1842 st055362 4.36 61.2 µM 1.38E-5 6 6.48E-6
6 SGTC_2688 kanamycin b 4.32 96.7 µM 1.66E-5 calcium & mitochondrial duress 320 7.91E-6
7 SGTC_3211 9128781 4.23 49.5 µM 2.42E-5 47 1.19E-5
8 SGTC_1259 0671-0132 4.09 43.0 µM 4.16E-5 22 2.13E-5
9 SGTC_260 nsc-65238 4.09 4.0 µM 4.27E-5 endomembrane recycling 14 2.19E-5
10 SGTC_2019 4016004 4.05 108.0 µM 5.04E-5 31 2.61E-5
11 SGTC_2561 acetosyringone 4.00 100.0 µM 6.08E-5 22 3.19E-5
12 SGTC_1833 st055984 4.00 71.0 µM 6.12E-5 16 3.22E-5
13 SGTC_2495 cafestol 3.81 54.5 µM 1.25E-4 Golgi 27 6.95E-5
14 SGTC_1080 nisoldipine 3.69 24.4 µM 1.92E-4 TSC3-RPN4 36 1.10E-4
15 SGTC_1797 st048695 3.69 56.8 µM 1.95E-4 NEO1 76 1.12E-4
16 SGTC_2416 tofa 3.65 880.0 nM 2.28E-4 sphingolipid biosynthesis & PDR1 62 1.32E-4
17 SGTC_1077 5-fluorocytosine 3.63 377.0 nM 2.45E-4 exosome 25 1.43E-4
18 SGTC_515 4092-1064 3.62 102.0 µM 2.48E-4 plasma membrane duress 68 1.45E-4
19 SGTC_2993 9054257 3.59 71.4 µM 2.83E-4 fatty acid desaturase (OLE1) 56 1.67E-4
20 SGTC_2558 carylophyllene oxide 3.35 100.0 µM 6.51E-4 37 4.08E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.129 6.48E-14 YNR020C ATP23 hom Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p
0.129 6.62E-14 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.123 6.96E-13 YBR174C_d YBR174C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective
0.122 1.32E-12 YDL162C_d YDL162C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly
0.121 2.35E-12 YDR101C ARX1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex
0.119 5.18E-12 YER180C-A SLO1 hom Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO
0.109 2.52E-10 YLR406C RPL31B hom Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication
0.107 4.46E-10 YKL033W TTI1 het Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies
0.103 2.18E-9 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.103 2.56E-9 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.101 5.18E-9 YKR028W SAP190 hom Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
0.101 5.23E-9 YER069W ARG5,6 hom Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
0.095 3.01E-8 YOR108W LEU9 hom Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication
0.095 3.15E-8 YPR140W TAZ1 hom Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; human ortholog tafazzin is implicated in Barth syndrome
0.094 4.35E-8 YER076C_p YER076C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization