YKL172W / EBP2

Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL172W / EBP2

Click on Significant Values for Screen Details ID:SGTC_118|Compound:0083-0097|FD-Score:3.91|P-value:3.45E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.93|P-value:3.22E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:4.89|P-value:1.11E-5 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:5.05|P-value:5.88E-6 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:12.70|P-value:1.45E-28 ID:SGTC_589|Compound:1227-0070|FD-Score:4.13|P-value:1.67E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:6.37|P-value:1.62E-8 ID:SGTC_615|Compound:k087-0205|FD-Score:4.61|P-value:3.19E-5 ID:SGTC_691|Compound:1548-0686|FD-Score:3.67|P-value:7.30E-4 ID:SGTC_906|Compound:1165-0455|FD-Score:4.57|P-value:3.65E-5 ID:SGTC_1074|Compound:carmofur|FD-Score:3.59|P-value:9.19E-4 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:5.55|P-value:7.42E-7 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:4.22|P-value:1.27E-4 ID:SGTC_2316|Compound:5844804|FD-Score:-3.88|P-value:3.70E-4 ID:SGTC_2430|Compound:5571206|FD-Score:-5.32|P-value:1.86E-6 ID:SGTC_2435|Compound:5706476|FD-Score:-3.99|P-value:2.59E-4 ID:SGTC_2794|Compound:7785382|FD-Score:-4.02|P-value:2.36E-4 ID:SGTC_2898|Compound:9056270|FD-Score:-4.29|P-value:9.47E-5 ID:SGTC_3297|Compound:9117502|FD-Score:-3.62|P-value:8.21E-4 ID:SGTC_3313|Compound:9133968|FD-Score:-4.53|P-value:3.95E-5 ID:SGTC_3314|Compound:9135860|FD-Score:-4.82|P-value:1.37E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:3.91|P-value:3.45E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.93|P-value:3.22E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:4.89|P-value:1.11E-5 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:5.05|P-value:5.88E-6 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:12.70|P-value:1.45E-28 ID:SGTC_589|Compound:1227-0070|FD-Score:4.13|P-value:1.67E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:6.37|P-value:1.62E-8 ID:SGTC_615|Compound:k087-0205|FD-Score:4.61|P-value:3.19E-5 ID:SGTC_691|Compound:1548-0686|FD-Score:3.67|P-value:7.30E-4 ID:SGTC_906|Compound:1165-0455|FD-Score:4.57|P-value:3.65E-5 ID:SGTC_1074|Compound:carmofur|FD-Score:3.59|P-value:9.19E-4 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:5.55|P-value:7.42E-7 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:4.22|P-value:1.27E-4 ID:SGTC_2316|Compound:5844804|FD-Score:-3.88|P-value:3.70E-4 ID:SGTC_2430|Compound:5571206|FD-Score:-5.32|P-value:1.86E-6 ID:SGTC_2435|Compound:5706476|FD-Score:-3.99|P-value:2.59E-4 ID:SGTC_2794|Compound:7785382|FD-Score:-4.02|P-value:2.36E-4 ID:SGTC_2898|Compound:9056270|FD-Score:-4.29|P-value:9.47E-5 ID:SGTC_3297|Compound:9117502|FD-Score:-3.62|P-value:8.21E-4 ID:SGTC_3313|Compound:9133968|FD-Score:-4.53|P-value:3.95E-5 ID:SGTC_3314|Compound:9135860|FD-Score:-4.82|P-value:1.37E-5

Top fitness defect scores for YKL172W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_423 5-fluorouracil 12.70 457.0 µM 1.45E-28 exosome 1 3.30E-37 2.32
2 SGTC_610 4215-0184 6.37 155.0 µM 1.62E-8 exosome 12 9.64E-11 0.71
3 SGTC_1077 5-fluorocytosine 5.55 377.0 nM 7.42E-7 exosome 12 1.47E-8 0.02
4 SGTC_261 5-fluorouridine 5.05 15.4 µM 5.88E-6 exosome 6 2.22E-7 0.37
5 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 4.89 4.6 µM 1.11E-5 heat shock/prefoldin 15 5.13E-7 0.06
6 SGTC_615 k087-0205 4.61 167.0 µM 3.19E-5 1 2.04E-6 0.56
7 SGTC_906 1165-0455 4.57 526.0 µM 3.65E-5 2 2.43E-6 0.78
8 SGTC_1928 4'-methoxychalcone 4.22 40.3 µM 1.27E-4 2 1.24E-5 0.62
9 SGTC_589 1227-0070 4.13 34.1 µM 1.67E-4 8 1.78E-5 0.14
10 SGTC_241 5hr heat shock (37°C) + cantharidin 3.93 155.4 µM 3.22E-4 cell wall signaling 15 4.19E-5 0.04
11 SGTC_118 0083-0097 3.91 135.3 µM 3.45E-4 translation 18 4.59E-5 0.03
12 SGTC_691 1548-0686 3.67 185.0 µM 7.30E-4 14 1.22E-4 0.05
13 SGTC_1074 carmofur 3.59 3.8 µM 9.19E-4 exosome 33 1.64E-4 0.03
14 SGTC_1467 k284-1082 3.47 293.0 µM 0.00131 13 2.60E-4 0.58
15 SGTC_611 3453-1652 3.46 23.7 µM 0.00134 31 2.68E-4 0.16
16 SGTC_1199 1638-0010 3.27 4.5 µM 0.00227 endomembrane recycling 9 5.35E-4 0.06
17 SGTC_590 k048-0076 3.16 22.4 µM 0.00303 RSC & ERG11 20 7.76E-4 0.16
18 SGTC_6 0844-0013 3.11 19.8 µM 0.00352 24 9.42E-4 0.00
19 SGTC_2484 5809818 3.09 12.0 µM 0.00368 8 9.98E-4 0.13
20 SGTC_830 4076-0286 3.08 57.8 µM 0.00382 translation 15 0.00105 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.417 2.79E-141 YGR195W SKI6 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.387 5.60E-120 YPL043W NOP4 het Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
0.380 9.80E-116 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.370 1.84E-109 YDL111C RRP42 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
0.340 2.25E-91 YOL021C DIS3 het Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
0.319 2.19E-80 YGR158C MTR3 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
0.291 1.29E-66 YPR143W RRP15 het Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
0.290 7.18E-66 YDR280W RRP45 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
0.277 5.94E-60 YPR142C_d YPR142C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
0.248 4.13E-48 YKL021C MAK11 het Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats
0.246 2.04E-47 YGR095C RRP46 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
0.206 1.77E-33 YDR060W MAK21 het Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
0.182 3.05E-26 YNL061W NOP2 het Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
0.167 1.87E-22 YNL002C RLP7 het Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
0.166 3.52E-22 YNL232W CSL4 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)