YKL173W / SNU114

GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL173W / SNU114

Click on Significant Values for Screen Details ID:SGTC_66|Compound:0109-0187|FD-Score:-3.36|P-value:5.00E-4 ID:SGTC_592|Compound:k241-0073|FD-Score:3.17|P-value:8.19E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:3.38|P-value:3.89E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:3.85|P-value:6.44E-5 ID:SGTC_1023|Compound:4482-3888|FD-Score:4.51|P-value:3.79E-6 ID:SGTC_1062|Compound:mesoridazine|FD-Score:-3.73|P-value:1.26E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:4.14|P-value:1.94E-5 ID:SGTC_1081|Compound:sertraline|FD-Score:3.81|P-value:7.78E-5 ID:SGTC_1089|Compound:terbinafine|FD-Score:-4.20|P-value:1.86E-5 ID:SGTC_1167|Compound:k781-0610|FD-Score:-3.94|P-value:5.59E-5 ID:SGTC_1170|Compound:0310-0127|FD-Score:4.00|P-value:3.58E-5 ID:SGTC_1173|Compound:0443-0020|FD-Score:-3.33|P-value:5.51E-4 ID:SGTC_1297|Compound:1068-0111|FD-Score:4.56|P-value:3.09E-6 ID:SGTC_1388|Compound:3346-4487|FD-Score:-3.55|P-value:2.47E-4 ID:SGTC_1753|Compound:st043925|FD-Score:4.81|P-value:9.36E-7 ID:SGTC_1754|Compound:st042065|FD-Score:-3.60|P-value:2.05E-4 ID:SGTC_1757|Compound:st041620|FD-Score:-5.52|P-value:2.92E-8 ID:SGTC_1764|Compound:st044502|FD-Score:-3.87|P-value:7.25E-5 ID:SGTC_1767|Compound:st044992|FD-Score:-4.35|P-value:9.89E-6 ID:SGTC_1834|Compound:st055981|FD-Score:3.80|P-value:8.05E-5 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.61|P-value:1.70E-4 ID:SGTC_1921|Compound:5740136|FD-Score:5.72|P-value:7.00E-9 ID:SGTC_2053|Compound:5265679|FD-Score:-3.83|P-value:8.42E-5 ID:SGTC_2301|Compound:7769476|FD-Score:3.75|P-value:9.83E-5 ID:SGTC_2302|Compound:7771753|FD-Score:4.04|P-value:3.04E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.58|P-value:2.72E-6 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.79|P-value:9.86E-5 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-3.40|P-value:4.34E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:3.36|P-value:4.21E-4 ID:SGTC_2824|Compound:7998404|FD-Score:5.81|P-value:4.22E-9 ID:SGTC_2834|Compound:9001755|FD-Score:-3.96|P-value:5.03E-5 ID:SGTC_2949|Compound:9072542|FD-Score:-3.87|P-value:7.33E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-6.64|P-value:3.22E-11 ID:SGTC_3065|Compound:9113888|FD-Score:3.30|P-value:5.17E-4 ID:SGTC_3090|Compound:9117696|FD-Score:-3.19|P-value:8.87E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:-3.36|P-value:5.00E-4 ID:SGTC_592|Compound:k241-0073|FD-Score:3.17|P-value:8.19E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:3.38|P-value:3.89E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:3.85|P-value:6.44E-5 ID:SGTC_1023|Compound:4482-3888|FD-Score:4.51|P-value:3.79E-6 ID:SGTC_1062|Compound:mesoridazine|FD-Score:-3.73|P-value:1.26E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:4.14|P-value:1.94E-5 ID:SGTC_1081|Compound:sertraline|FD-Score:3.81|P-value:7.78E-5 ID:SGTC_1089|Compound:terbinafine|FD-Score:-4.20|P-value:1.86E-5 ID:SGTC_1167|Compound:k781-0610|FD-Score:-3.94|P-value:5.59E-5 ID:SGTC_1170|Compound:0310-0127|FD-Score:4.00|P-value:3.58E-5 ID:SGTC_1173|Compound:0443-0020|FD-Score:-3.33|P-value:5.51E-4 ID:SGTC_1297|Compound:1068-0111|FD-Score:4.56|P-value:3.09E-6 ID:SGTC_1388|Compound:3346-4487|FD-Score:-3.55|P-value:2.47E-4 ID:SGTC_1753|Compound:st043925|FD-Score:4.81|P-value:9.36E-7 ID:SGTC_1754|Compound:st042065|FD-Score:-3.60|P-value:2.05E-4 ID:SGTC_1757|Compound:st041620|FD-Score:-5.52|P-value:2.92E-8 ID:SGTC_1764|Compound:st044502|FD-Score:-3.87|P-value:7.25E-5 ID:SGTC_1767|Compound:st044992|FD-Score:-4.35|P-value:9.89E-6 ID:SGTC_1834|Compound:st055981|FD-Score:3.80|P-value:8.05E-5 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.61|P-value:1.70E-4 ID:SGTC_1921|Compound:5740136|FD-Score:5.72|P-value:7.00E-9 ID:SGTC_2053|Compound:5265679|FD-Score:-3.83|P-value:8.42E-5 ID:SGTC_2301|Compound:7769476|FD-Score:3.75|P-value:9.83E-5 ID:SGTC_2302|Compound:7771753|FD-Score:4.04|P-value:3.04E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.58|P-value:2.72E-6 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.79|P-value:9.86E-5 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-3.40|P-value:4.34E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:3.36|P-value:4.21E-4 ID:SGTC_2824|Compound:7998404|FD-Score:5.81|P-value:4.22E-9 ID:SGTC_2834|Compound:9001755|FD-Score:-3.96|P-value:5.03E-5 ID:SGTC_2949|Compound:9072542|FD-Score:-3.87|P-value:7.33E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-6.64|P-value:3.22E-11 ID:SGTC_3065|Compound:9113888|FD-Score:3.30|P-value:5.17E-4 ID:SGTC_3090|Compound:9117696|FD-Score:-3.19|P-value:8.87E-4

Top fitness defect scores for YKL173W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2824 7998404 5.81 52.0 µM 4.22E-9 3 3.11E-9 0.39
2 SGTC_1921 5740136 5.72 50.0 µM 7.00E-9 TRP & mitochondrial translation 5 5.21E-9 0.13
3 SGTC_1753 st043925 4.81 64.0 µM 9.36E-7 1 7.73E-7 0.79
4 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 4.58 3.7 µM 2.72E-6 11 2.29E-6 0.54
5 SGTC_1297 1068-0111 4.56 14.1 µM 3.09E-6 1 2.61E-6 0.39
6 SGTC_1023 4482-3888 4.51 16.0 µM 3.79E-6 TSC3-RPN4 6 3.22E-6 0.19
7 SGTC_1079 idarubicin 4.14 2.4 µM 1.94E-5 DNA damage response 6 1.71E-5 0.41
8 SGTC_2302 7771753 4.04 200.0 µM 3.04E-5 6 2.69E-5 0.06
9 SGTC_1170 0310-0127 4.00 64.8 µM 3.58E-5 8 3.18E-5 0.04
10 SGTC_852 0418-0143 3.85 439.0 µM 6.44E-5 calcium & mitochondrial duress 15 5.79E-5 0.14
11 SGTC_1081 sertraline 3.81 6.0 µM 7.78E-5 60S ribosome export 8 7.02E-5 0.36
12 SGTC_1834 st055981 3.80 52.8 µM 8.05E-5 RPP1 & pyrimidine depletion 9 7.26E-5 0.05
13 SGTC_2301 7769476 3.75 200.0 µM 9.83E-5 14 8.90E-5 0.05
14 SGTC_1890 3',4',7',8'-tetrahydroxyflavone 3.61 14.9 µM 1.70E-4 9 1.55E-4 0.12
15 SGTC_845 0330-0138 3.38 4.5 µM 3.89E-4 11 3.62E-4 0.11
16 SGTC_2754 granisetron 3.36 64.0 µM 4.21E-4 9 3.92E-4 0.29
17 SGTC_3065 9113888 3.30 49.5 µM 5.17E-4 12 4.83E-4 0.01
18 SGTC_592 k241-0073 3.17 46.5 µM 8.19E-4 8 7.72E-4 0.03
19 SGTC_2701 st077767 3.10 14.7 µM 0.00103 19 9.73E-4 0.08
20 SGTC_1783 st039459 3.08 87.5 µM 0.00108 Golgi 18 0.00102 0.20

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.164 1.55E-21 YKL202W_d YKL202W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.132 1.77E-14 YLR314C CDC3 het Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells
0.127 1.37E-13 YLR026C SED5 het cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
0.124 6.37E-13 YOR218C_d YOR218C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W
0.121 1.68E-12 YBL040C ERD2 het HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins
0.116 1.47E-11 YKL117W SBA1 hom Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress
0.105 1.22E-9 YBR193C MED8 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.104 1.32E-9 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.104 1.78E-9 YGL004C RPN14 hom Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
0.103 1.89E-9 YKL083W_d YKL083W_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
0.102 3.49E-9 YBL020W RFT1 het Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein
0.101 3.92E-9 YKL102C_d YKL102C_d hom Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site
0.100 6.25E-9 YER018C SPC25 het Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
0.099 8.39E-9 YFL046W_p FMP32_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.099 1.02E-8 YCR013C_d YCR013C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant