YKL182W / FAS1

Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL182W / FAS1

Click on Significant Values for Screen Details ID:SGTC_1|Compound:0258-0098|FD-Score:8.43|P-value:1.69E-10 ID:SGTC_81|Compound:0411-0046|FD-Score:15.10|P-value:1.35E-29 ID:SGTC_103|Compound:0844-0104|FD-Score:12.20|P-value:4.90E-20 ID:SGTC_157|Compound:1348-1695|FD-Score:7.97|P-value:1.41E-9 ID:SGTC_195|Compound:4234-0266|FD-Score:5.52|P-value:1.90E-5 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:57.30|P-value:0 ID:SGTC_266|Compound:formosanin C|FD-Score:11.30|P-value:1.71E-17 ID:SGTC_302|Compound:1094-0063|FD-Score:5.31|P-value:3.66E-5 ID:SGTC_353|Compound:1988-1175|FD-Score:7.16|P-value:4.77E-8 ID:SGTC_366|Compound:0181-0206|FD-Score:5.26|P-value:4.24E-5 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:6.53|P-value:5.58E-7 ID:SGTC_435|Compound:c-PAF|FD-Score:4.92|P-value:1.20E-4 ID:SGTC_455|Compound:paf C-16|FD-Score:5.53|P-value:1.84E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:6.21|P-value:1.80E-6 ID:SGTC_531|Compound:3788-1495|FD-Score:13.30|P-value:2.51E-23 ID:SGTC_628|Compound:1636-0253|FD-Score:4.37|P-value:5.35E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:4.23|P-value:7.79E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:7.25|P-value:3.20E-8 ID:SGTC_966|Compound:1300-0360|FD-Score:4.46|P-value:4.25E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:4.24|P-value:7.63E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:4.27|P-value:6.99E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:4.39|P-value:5.19E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:9.91|P-value:7.94E-14 ID:SGTC_1146|Compound:3579-1023|FD-Score:5.14|P-value:6.24E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:5.53|P-value:1.80E-5 ID:SGTC_1183|Compound:1254-0324|FD-Score:-4.37|P-value:6.53E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:4.59|P-value:3.03E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:4.47|P-value:4.14E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:4.15|P-value:9.54E-4 ID:SGTC_1508|Compound:1080-0625|FD-Score:-4.49|P-value:4.78E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:6.58|P-value:4.64E-7 ID:SGTC_1588|Compound:farnesol|FD-Score:4.58|P-value:3.11E-4 ID:SGTC_1590|Compound:st077108|FD-Score:-4.44|P-value:5.42E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:4.54|P-value:3.43E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:6.69|P-value:3.02E-7 ID:SGTC_1682|Compound:st012968|FD-Score:4.23|P-value:7.83E-4 ID:SGTC_1707|Compound:st032323|FD-Score:4.36|P-value:5.56E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:5.78|P-value:7.93E-6 ID:SGTC_1941|Compound:st076221|FD-Score:6.40|P-value:9.13E-7 ID:SGTC_2050|Compound:5263056|FD-Score:-6.82|P-value:2.50E-7 ID:SGTC_2100|Compound:5377183|FD-Score:4.97|P-value:1.02E-4 ID:SGTC_2244|Compound:6635614|FD-Score:4.46|P-value:4.28E-4 ID:SGTC_2331|Compound:7980721|FD-Score:-4.53|P-value:4.31E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:20.40|P-value:2.45E-52 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:23.80|P-value:5.67E-70 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-4.47|P-value:5.02E-4 ID:SGTC_2601|Compound:securinine|FD-Score:4.93|P-value:1.15E-4 ID:SGTC_2602|Compound:kawain|FD-Score:-5.83|P-value:8.77E-6 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:5.11|P-value:6.73E-5 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:5.83|P-value:6.77E-6 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:4.17|P-value:9.05E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:9.94|P-value:6.97E-14 ID:SGTC_2858|Compound:9025781|FD-Score:6.97|P-value:9.96E-8 ID:SGTC_2997|Compound:9067075|FD-Score:7.92|P-value:1.82E-9 ID:SGTC_3301|Compound:9125354|FD-Score:5.51|P-value:1.93E-5 ID:SGTC_3311|Compound:9131146|FD-Score:6.28|P-value:1.41E-6 ID:SGTC_3350|Compound:9150499|FD-Score:4.61|P-value:2.80E-4 ID:SGTC_1|Compound:0258-0098|FD-Score:8.43|P-value:1.69E-10 ID:SGTC_81|Compound:0411-0046|FD-Score:15.10|P-value:1.35E-29 ID:SGTC_103|Compound:0844-0104|FD-Score:12.20|P-value:4.90E-20 ID:SGTC_157|Compound:1348-1695|FD-Score:7.97|P-value:1.41E-9 ID:SGTC_195|Compound:4234-0266|FD-Score:5.52|P-value:1.90E-5 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:57.30|P-value:0 ID:SGTC_266|Compound:formosanin C|FD-Score:11.30|P-value:1.71E-17 ID:SGTC_302|Compound:1094-0063|FD-Score:5.31|P-value:3.66E-5 ID:SGTC_353|Compound:1988-1175|FD-Score:7.16|P-value:4.77E-8 ID:SGTC_366|Compound:0181-0206|FD-Score:5.26|P-value:4.24E-5 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:6.53|P-value:5.58E-7 ID:SGTC_435|Compound:c-PAF|FD-Score:4.92|P-value:1.20E-4 ID:SGTC_455|Compound:paf C-16|FD-Score:5.53|P-value:1.84E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:6.21|P-value:1.80E-6 ID:SGTC_531|Compound:3788-1495|FD-Score:13.30|P-value:2.51E-23 ID:SGTC_628|Compound:1636-0253|FD-Score:4.37|P-value:5.35E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:4.23|P-value:7.79E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:7.25|P-value:3.20E-8 ID:SGTC_966|Compound:1300-0360|FD-Score:4.46|P-value:4.25E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:4.24|P-value:7.63E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:4.27|P-value:6.99E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:4.39|P-value:5.19E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:9.91|P-value:7.94E-14 ID:SGTC_1146|Compound:3579-1023|FD-Score:5.14|P-value:6.24E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:5.53|P-value:1.80E-5 ID:SGTC_1183|Compound:1254-0324|FD-Score:-4.37|P-value:6.53E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:4.59|P-value:3.03E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:4.47|P-value:4.14E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:4.15|P-value:9.54E-4 ID:SGTC_1508|Compound:1080-0625|FD-Score:-4.49|P-value:4.78E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:6.58|P-value:4.64E-7 ID:SGTC_1588|Compound:farnesol|FD-Score:4.58|P-value:3.11E-4 ID:SGTC_1590|Compound:st077108|FD-Score:-4.44|P-value:5.42E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:4.54|P-value:3.43E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:6.69|P-value:3.02E-7 ID:SGTC_1682|Compound:st012968|FD-Score:4.23|P-value:7.83E-4 ID:SGTC_1707|Compound:st032323|FD-Score:4.36|P-value:5.56E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:5.78|P-value:7.93E-6 ID:SGTC_1941|Compound:st076221|FD-Score:6.40|P-value:9.13E-7 ID:SGTC_2050|Compound:5263056|FD-Score:-6.82|P-value:2.50E-7 ID:SGTC_2100|Compound:5377183|FD-Score:4.97|P-value:1.02E-4 ID:SGTC_2244|Compound:6635614|FD-Score:4.46|P-value:4.28E-4 ID:SGTC_2331|Compound:7980721|FD-Score:-4.53|P-value:4.31E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:20.40|P-value:2.45E-52 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:23.80|P-value:5.67E-70 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-4.47|P-value:5.02E-4 ID:SGTC_2601|Compound:securinine|FD-Score:4.93|P-value:1.15E-4 ID:SGTC_2602|Compound:kawain|FD-Score:-5.83|P-value:8.77E-6 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:5.11|P-value:6.73E-5 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:5.83|P-value:6.77E-6 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:4.17|P-value:9.05E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:9.94|P-value:6.97E-14 ID:SGTC_2858|Compound:9025781|FD-Score:6.97|P-value:9.96E-8 ID:SGTC_2997|Compound:9067075|FD-Score:7.92|P-value:1.82E-9 ID:SGTC_3301|Compound:9125354|FD-Score:5.51|P-value:1.93E-5 ID:SGTC_3311|Compound:9131146|FD-Score:6.28|P-value:1.41E-6 ID:SGTC_3350|Compound:9150499|FD-Score:4.61|P-value:2.80E-4

Top fitness defect scores for YKL182W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_226 1,3-diallylurea 57.30 8.3 mM 0 1 0 50.50
2 SGTC_2567 aureobasidin a 23.80 60.0 nM 5.67E-70 5 4.85E-125 6.86
3 SGTC_2417 cerulenin 20.40 830.0 nM 2.45E-52 1 4.46E-93 11.60
4 SGTC_81 0411-0046 15.10 10.0 µM 1.35E-29 2 6.46E-52 2.84
5 SGTC_531 3788-1495 13.30 174.0 µM 2.51E-23 unfolded protein response 1 1.38E-40 4.37
6 SGTC_103 0844-0104 12.20 51.8 µM 4.90E-20 ERAD & cell cycle 7 1.19E-34 0.32
7 SGTC_266 formosanin C 11.30 406.0 nM 1.71E-17 sphingolipid biosynthesis & PDR1 3 4.55E-30 3.64
8 SGTC_2766 oltipraz 9.94 83.9 µM 6.97E-14 heme requiring 3 1.44E-23 0.28
9 SGTC_1133 3473-0247 9.91 50.0 µM 7.94E-14 RSC complex & mRNA processing 7 1.81E-23 0.03
10 SGTC_1 0258-0098 8.43 57.4 µM 1.69E-10 heme biosynthesis & mitochondrial translocase 2 1.71E-17 0.15
11 SGTC_157 1348-1695 7.97 65.8 µM 1.41E-9 iron homeostasis 14 7.67E-16 0.32
12 SGTC_2997 9067075 7.92 71.4 µM 1.82E-9 unfolded protein response 4 1.21E-15 0.12
13 SGTC_829 1635-0120 7.25 117.0 µM 3.20E-8 DNA damage response 20 2.03E-13 0.14
14 SGTC_353 1988-1175 7.16 58.3 µM 4.77E-8 unfolded protein response 1 4.13E-13 1.87
15 SGTC_2858 9025781 6.97 9.0 µM 9.96E-8 sphingolipid biosynthesis & PDR1 3 1.53E-12 0.65
16 SGTC_1679 4130-1276 6.69 6.7 µM 3.02E-7 Golgi 12 1.11E-11 0.12
17 SGTC_1568 digitonin 6.58 820.0 nM 4.64E-7 sphingolipid biosynthesis & PDR1 3 2.38E-11 0.77
18 SGTC_434 1-hexadecyl-2-methylglycero-3 PC 6.53 8.0 µM 5.58E-7 5 3.30E-11 0.39
19 SGTC_1941 st076221 6.40 61.8 µM 9.13E-7 sphingolipid biosynthesis & PDR1 2 7.92E-11 1.16
20 SGTC_3311 9131146 6.28 2.9 µM 1.41E-6 cell wall 6 1.70E-10 0.55

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.355 4.51E-100 YDR123C INO2 hom Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion
0.338 1.55E-90 YMR222C FSH2 hom Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2
0.262 7.55E-54 YMR211W DML1 het Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
0.249 1.12E-48 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.225 1.31E-39 YDR530C APA2 hom Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication
0.165 5.84E-22 YMR102C_p YMR102C_p hom Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene
0.158 3.22E-20 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.156 1.17E-19 YDR326C YSP2 hom Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication
0.151 1.81E-18 YNL257C SIP3 hom Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication
0.148 8.24E-18 YIL109C SEC24 het Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p
0.143 9.62E-17 YNL026W SAM50 het Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85
0.137 1.81E-15 YCL069W VBA3 hom Permease of basic amino acids in the vacuolar membrane
0.135 3.98E-15 YDR118W APC4 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
0.132 1.97E-14 YOR061W CKA2 hom Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching
0.131 2.23E-14 YBR159W IFA38 hom Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides